Although Cherokee is known to show highly flexible clausal word order, the principles that predict speakers' preferences among possible orders are not extensively described. This article presents a new description of the grammatical properties that predict clausal word order in spoken Cherokee, based on a corpus study of word-order variation. Our results show that the placement of nominal expressions relative to verbs, and the relative order of nominal expressions within a clause, are determined in a probabilistic way by the cumulative interaction of several factors: REFERENTIAL ACCESSIBILITY, CONTRAST, AND THEMATIC ROLE. The findings suggest that thematic properties may have a greater word-order role than generally assumed in languages with nonconfigurational and/or polysynthetic properties.