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Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi I relationships and genetic diversity

Published online by Cambridge University Press:  03 May 2019

César Gómez-Hernández ,
Sergio D. Pérez ,
Karine Rezende-Oliveira ,
Cecilia G. Barbosa ,
Eliane Lages-Silva ,
Luis Eduardo Ramírez  and
Juan David Ramírez Open the ORCID record for Juan David Ramírez [Opens in a new window]
César Gómez-Hernández
Affiliation:
Laboratorio de Parasitologia, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil
Sergio D. Pérez
Affiliation:
Grupo de Investigaciones Microbiológicas-UR (GIMUR), Programa de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Bogotá, Colombia
Karine Rezende-Oliveira
Affiliation:
Laboratorio de Ciências Biomédicas, Universidade Federal de Uberlândia, Campus do Pontal, Ituiutaba, Minas Gerais, Brazil
Cecilia G. Barbosa
Affiliation:
Laboratorio de Parasitologia, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil
Eliane Lages-Silva
Affiliation:
Laboratorio de Parasitologia, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil
Luis Eduardo Ramírez
Affiliation:
Laboratorio de Parasitologia, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil
Juan David Ramírez*
Affiliation:
Grupo de Investigaciones Microbiológicas-UR (GIMUR), Programa de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad del Rosario, Bogotá, Colombia
*
Author for correspondence: Juan David Ramírez, E-mail: juand.ramirez@urosario.edu.co
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Abstract

Chagas Disease is a zoonosis caused by the parasite Trypanosoma cruzi. Several high-resolution markers have subdivided T. cruzi taxon into at least seven lineages or Discrete Typing Units (DTUs) (TcI-TcVI and TcBat). Trypanosoma cruzi I is the most diverse and geographically widespread DTU. Recently a TcI genotype related to domestic cycles was proposed and named as TcIDOM. Herein, we combined traditional markers and housekeeping genes and applied a Multispecies Coalescent method to explore intra-TcI relationships, lineage boundaries and genetic diversity in a random set of isolates and DNA sequences retrieved from Genbank from different countries in the Americas. We found further evidence supporting TcIDOM as an independent and emerging genotype of TcI at least in Colombia and Venezuela. We also found evidence of high phylogenetic incongruence between parasite's gene trees (including introgression) and embedded species trees, and a lack of genetic structure among geography and hosts, illustrating the complex dynamics and epidemiology of TcI across the Americas. These findings provide novel insights into T. cruzi systematics and epidemiology and support the need to assess parasite diversity and lineage boundaries through hypothesis testing using different approaches to those traditionally employed, including the Bayesian Multispecies coalescent method.

Keywords

Chagas diseasediscrete typing unitgenotypehousekeeping geneslineagemultispecies coalescent

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Type
Research Article
Information
Parasitology , Volume 146 , Issue 8 , July 2019 , pp. 1063 - 1074
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Copyright © Cambridge University Press 2019 

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Footnotes

*

These authors made equal contributions.

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