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Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens

Published online by Cambridge University Press:  05 July 2011

B. Zhang ,
X. Yang ,
Y. Guo  and
F. Long
B. Zhang
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China
X. Yang
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China State Key Laboratory of Biological Feed Engineering, Beijing Dabeinong Technology Group Co. Ltd, Beijing, China
Y. Guo*
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China
F. Long
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China
*
†E-mail: guoym9899@yahoo.com.cn
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Abstract

The effect of dietary lipids and Clostridium butyricum supplementation on circulating lipids and the transcription of selected genes involved in lipid metabolism were investigated in broiler chickens. One hundred and sixty-day-old broilers (Arbor Acres) were divided into four treatment groups in a 2 × 2 factorial arrangement and fed four diets with two lipid sources (soybean oil or fish oil) and two levels of C. butyricum (0 or 5 g/kg) for a period of 6 weeks. Serum concentrations of total cholesterol and low-density lipoprotein cholesterol were lower (P < 0.05) in broilers fed diets containing fish oil than in those fed diets containing soybean oil. Fish oil significantly reduced (P < 0.05) hepatic fatty acid synthase and lipoprotein lipase (LPL) activities in abdominal fat. In contrast, fish oil significantly increased (P < 0.05) breast muscle LPL activity and hepatic peroxisome proliferator-activated receptor-α and LPL gene expression. The addition of C. butyricum significantly increased (P < 0.05) LPL activity in abdominal fat and liver-type fatty acid-binding protein gene expression in jejunal mucosa. The results of this study indicated that the reduced abdominal fat in broilers fed fish oil as observed may be due to augmented hepatic fatty acid catabolism and lower hepatic fat synthesis. The increased intramuscular fat content in breast muscle of broilers fed C. butyricum as described may be the result of enhanced fatty acid uptake.

Keywords

Clostridium butyricumfish oillipid-related genes expressionbroiler chicken
Type
Full Paper
Information
animal , Volume 5 , Issue 12 , 10 November 2011 , pp. 1909 - 1915
Copyright
Copyright © The Animal Consortium 2011

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Footnotes

a

Both authors have contributed equally.

References

An, BK, Banno, C, Xia, ZS, Tanaka, K, Ohtani, S 1997. Effects of dietary fat sources on lipid metabolism in growing chicks (Gallus domesticus). Comparative Biochemistry and Physiology B – Biochemistry and Molecular Biology 116, 119–125.Google ScholarPubMed
Back, DW, Goldman, MJ, Fisch, JE 1986. The fatty acid synthase gene in avian liver. Two mRNAs are expressed and regulated in parallel by feeding, primarily at the level of transcription. Journal of Biological Chemistry 261, 4190–4197.CrossRefGoogle ScholarPubMed
Bradford, MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Brown, MS, Goldstein, JL 1997. The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89, 331–340.CrossRefGoogle ScholarPubMed
Castillo, M, Amalik, F, Linares, A, Garcia-Peregrin, E 2000. Fish oil reduces cholesterol and arachidonic acid levels in plasma and lipoproteins from hypercholesterolemic chicks. Molecular and Cellular Biochemistry 210, 121–130.CrossRefGoogle ScholarPubMed
Corcoran, MP, Lamon-Fava, S, Fielding, RA 2007. Skeletal muscle lipid deposition and insulin resistance: effect of dietary fatty acids and exercise. American Journal of Clinical Nutrition 85, 662–677.Google ScholarPubMed
Daval, S, Lagarrigue, S, Douaire, M 2000. Messenger RNA levels and transcription rates of hepatic lipogenesis genes in genetically lean and fat chickens. Genetics Selection Evolution 32, 521–531.CrossRefGoogle ScholarPubMed
Endo, T, Nakano, M, Shimizu, S, Fukushima, M, Miyoshi, S 1999. Effects of a probiotic on the lipid metabolism of cocks fed on a cholesterol-enriched diet. Bioscience, Biotechnology and Biochemistry 63, 1569–1575.CrossRefGoogle ScholarPubMed
Fébel, H, Mézes, M, Pálfy, T, Hermán, A, Gundel, J, Lugasi, A, Balogh, K, Kocsis, I, Blázovics, A 2008. Effect of dietary fatty acid pattern on growth, body fat composition and antioxidant parameters in broilers. Journal of Animal Physiology and Animal Nutrition 92, 369–376.CrossRefGoogle ScholarPubMed
Goldberg, IJ 1996. Lipoprotein lipase and lipolysis: central roles in lipoprotein metabolism and atherogenesis. Journal of Lipid Research 37, 693–707.CrossRefGoogle ScholarPubMed
O'Hea, EK, Leveille, GA 1968. Lipogenesis in isolated adipose tissue of the domestic chick (Gallus domesticus). Comparative Biochemistry and Physiology 26, 111–120.CrossRefGoogle ScholarPubMed
He, X, Yang, X, Guo, Y 2007. Effects of different dietary oil sources on immune function in cyclophosphamide immunosuppressed chickens. Animal Feed Science and Technology 139, 186–200.CrossRefGoogle Scholar
Hillgartner, FB, Salati, LM, Goodridge, AG 1995. Physiological and molecular mechanisms involved in nutritional regulation of fatty acid synthesis. Physiological Reviews 75, 47–76.CrossRefGoogle ScholarPubMed
Hulan, HW, Ackman, RG, Ratnayake, WM, Proudfoot, FG 1989. Omega-3 fatty acid levels and general performance of commercial broilers fed practical levels of redfish meal. Poultry Science 68, 153–162.CrossRefGoogle ScholarPubMed
Isono, A, Katsuno, T, Sato, T, Nakagawa, T, Kato, Y, Sato, N, Seo, G, Suzuki, Y, Saito, Y 2007. Clostridium butyricum TO-A culture supernatant downregulates TLR4 in human colonic epithelial cells. Digestive Diseases and Sciences 52, 2963–2971.CrossRefGoogle ScholarPubMed
Ito, I, Hayashi, T, Iguchi, A, Endo, H, Nakao, M, Kato, S, Nabeshima, T, Ogura, Y 1997. Effects of administration of clostridium butyricum to patients receiving long-term feeding. Japanese Journal of Geriatrics 34, 298–304.Google Scholar
Kersten, S 2001. Mechanisms of nutritional and hormonal regulation of lipogenesis. EMBO Reports 2, 282–286.CrossRefGoogle ScholarPubMed
Kim, HJ, Takahashi, M, Ezaki, O 1999. Fish oil feeding decreases mature sterol regulatory element-binding protein 1 (SREBP-1) by down-regulation of SREBP-1c mRNA in mouse liver. A possible mechanism for down-regulation of lipogenic enzyme mRNAs. Journal of Biological Chemistry 274, 25892–25898.CrossRefGoogle ScholarPubMed
Kim, JB, Sarraf, P, Wright, M, Yao, KM, Mueller, E, Solanes, G, Lowell, BB, Spiegelman, BM 1998. Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1. Journal of Clinical Investigation 101, 1–9.CrossRefGoogle ScholarPubMed
Krämer, DK, Al-Khalili, L, Guigas, B, Leng, Y, Garcia-Roves, PM, Krook, A 2007. Role of AMP kinase and PPARδ in the regulation of lipid and glucose metabolism in human skeletal muscle. Journal of Biological Chemistry 282, 19313–19320.CrossRefGoogle ScholarPubMed
Meng, H, Li, H, Zhao, JG, Gu, ZL 2005. Differential expression of peroxisome proliferator-activated receptors alpha and gamma gene in various chicken tissues. Domestic Animal Endocrinology 28, 105–110.CrossRefGoogle ScholarPubMed
Moibi, JA, Ekpe, ED, Christopherson, RJ 2000. Acetyl-CoA carboxylase and fatty acid synthase activity and immunodetectable protein in adipose tissues of ruminants: effect of temperature and feeding level. Journal of Animal Science 78, 2383–2392.CrossRefGoogle ScholarPubMed
Mossab, A, Lessire, M, Guillaumin, S, Kouba, M, Mourot, J, Peiniau, P, Hermier, D 2002. Effect of dietary fats on hepatic lipid metabolism in the growing turkey. Comparative Biochemistry and Physiology B – Biochemistry and Molecular Biology 132, 473–483.CrossRefGoogle ScholarPubMed
Nakatani, T, Tsuboyama-Kasaoka, N, Takahashi, M, Miura, S, Ezaki, O 2002. Mechanism for peroxisome proliferator-activated receptor-α activator-induced up-regulation of UCP2 mRNA in rodent hepatocytes. Journal of Biological Chemistry 277, 9562–9569.CrossRefGoogle ScholarPubMed
Ponte, PIP, Prates, JAM, Crespo, JP, Crespo, DG, Mourão, JL, Alves, SP, Bessa, RJB, Chaveiro-Soares, MA, Gama, LT, Ferreira, LMA, Fontes, CMGA 2008. Restricting the intake of a cereal-based feed in free-range-pastured poultry: effects on performance and meat quality. Poultry Science 87, 2032–2042.CrossRefGoogle ScholarPubMed
Prows, DR, Murphy, EJ, Schroeder, F 1995. Intestinal and liver fatty acid binding proteins differentially affect fatty acid uptake and esterification in L-cells. Lipids 30, 907–910.CrossRefGoogle ScholarPubMed
Ren, B, Thelen, AP, Peters, JM, Gonzalez, FJ, Jump, DB 1997. Polyunsaturated fatty acid suppression of hepatic fatty acid synthase and S14 gene expression does not require peroxisome proliferator-activated receptor α. Journal of Biological Chemistry 272, 26827–26832.CrossRefGoogle Scholar
Ribeiro, A, Mangeney, M, Cardot, P, Loriette, C, Rayssiguier, Y, Chambaz, J, Bereziat, G 1991. Effect of dietary fish oil and corn-oil on lipid-metabolism and apolipoprotein gene-expression by rat-liver. European Journal of Biochemistry 196, 499–507.CrossRefGoogle ScholarPubMed
Richieri, GV, Ogata, RT, Kleinfeld, AM 1994. Equilibrium constants for the binding of fatty acids with fatty acid- binding proteins from adipocyte, intestine, heart, and liver measured with the fluorescent probe ADIFAB. Journal of Biological Chemistry 269, 23918–23930.CrossRefGoogle ScholarPubMed
Sanz, M, Lopez-Bote, CJ, Menoyo, D, Bautista, JM 2000. Abdominal fat deposition and fatty acid synthesis are lower and β-oxidation is higher in broiler chickens fed diets containing unsaturated rather than saturated fat. Journal of Nutrition 130, 3034–3037.CrossRefGoogle ScholarPubMed
Seki, H, Shiohara, M, Matsumura, T, Miyagawa, N, Tanaka, M, Komiyama, A, Kurata, S 2003. Prevention of antibiotic-associated diarrhea in children by Clostridium butyricum MIYAIRI. Pediatrics International 45, 86–90.CrossRefGoogle ScholarPubMed
Vassileva, G, Huwyler, L, Poirier, K, Agellon, LB, Toth, MJ 2000. The intestinal fatty acid binding protein is not essential for dietary fat absorption in mice. FASEB Journal 14, 2040–2046.CrossRefGoogle Scholar
Voshol, PJ, Jong, MC, Dahlmans, VEH, Kratky, D, Levak-Frank, S, Zechner, R, Romijn, JA, Havekes, LM 2001. In muscle-specific lipoprotein lipase-overexpressing mice, muscle triglyceride content is increased without inhibition of insulin-stimulated whole-body and muscle-specific glucose uptake. Diabetes 50, 2585–2590.CrossRefGoogle ScholarPubMed
Wong, SH, Nestel, PJ, Trimble, RP 1984. The adaptive effects of dietary fish and safflower oil on lipid and lipoprotein metabolism in perfused rat liver. Biochimica et Biophysica Acta – Lipids and Lipid Metabolism 792, 103–109.CrossRefGoogle ScholarPubMed
Xu, J, Nakamura, MT, Cho, HP, Clarke, SD 1999. Sterol regulatory element binding protein-1 expression is suppressed by dietary polyunsaturated fatty acids. A mechanism for the coordinate suppression of lipogenic genes by polyunsaturated fats. Journal of Biological Chemistry 274, 23577–23583.CrossRefGoogle ScholarPubMed
Yang, X, Zhang, B, Guo, Y, Jiao, P, Long, F 2010. Effects of dietary lipids and Clostridium butyricum on fat deposition and meat quality of broiler chickens. Poultry Science 89, 254–260.CrossRefGoogle ScholarPubMed
Yu, YH, Wang, PH, Cheng, WTK, Mersmann, HJ, Wu, SC, Ding, ST 2010. Porcine peroxisome proliferator-activated receptor {delta} mediates the lipolytic effects of dietary fish oil to reduce body fat deposition. Journal of Animal Science 88, 2009–2018.CrossRefGoogle ScholarPubMed