A significant number of lambs born each year in Australia die within 72 h of birth. Periods of high wind, combined with rain and low temperatures can lead to marked increases in the mortality level. Under these climatic conditions mortality levels may be reduced with the provision of shelter. This study used contact loggers to compare interactions between ewes with twin lambs across two shelter types (Hessian and shrubs), while also comparing ewes with single and twin lambs in a single shelter type (Hessian). The contact loggers record the time of the initial contact (within approximately 4 to 5 m) between collared animals and the duration of each contact. Contact levels between ewes immediately after lambing were only 10% of the initial levels (1 h/day). For single-born lambs, lambs averaged 11 h contact per day with their mother, while for twinborn lambs, each lamb averaged 9.25 h/day with its mother and 14.7 h/day with its sibling. The level of contact between ewes and each of their offspring in the Hessian was 24% lower (P < 0.05) for ewes with twin lambs than with singles. For ewes with twin lambs the level of contact was 17% lower (P < 0.05) in the Hessian shelter compared with shrub shelter. We conclude that shelter type and birth number can affect the level of contact between ewes and their offspring.

Housing conditions for horses impose various levels of confinement, which may compromise welfare. Lying behaviour and activity can be used as welfare indicators for domestic animals and rebound behaviour suggests a build-up of motivation resulting from deprivation. The objective of this study was to determine if activity and lying behaviour of horses are affected by housing conditions and to investigate the occurrence of rebound behaviour after release from confinement. Eight horses were subjected, in pairs, to each of four experimental treatments; paddock (P), fully stabled (FS), partly stabled (PS) and yard (Y). Each horse received 6 days acclimatisation prior to the 24 h recording period. Time spent in lying and activity were electronically recorded using a tilt switch and motion sensor connected to a data logger worn on the horse’s left foreleg. Time spent active during the first 5 min of release from stable to paddock in the PS treatment (days 1 and 5) and at the same time of day in the P treatment was used as a measure of rebound behaviour. Effect of housing conditions on total time spent active was highly significant (FS = 123 s, PS = 158 s, Y = 377 s, P = 779 s, P < 0.001). Housing conditions did not significantly affect total time spent lying (P = 0.646). Horses were significantly more active, compared with baseline paddock behaviour, on release from stabling on both days 1 (P = 0.006) and 5 (P = 0.025) of PS treatment. These results suggest that activity patterns of horses, but not lying behaviour, are affected by the housing conditions tested and that rebound activity occurs in horses after a period of confinement.

For many years, the breeding value estimation for Swedish riding horses has been based on results from Riding Horse Quality Tests (RHQTs) of 4-year-olds only. Traits tested are conformation, gaits and jumping ability. An integrated index including competition results is under development to both get as reliable proofs as possible and increases the credibility of the indexes among breeders, trainers and riders. The objectives of this study were to investigate the suitability of competition data for use in genetic evaluations of horses and to examine how well young horse performance agrees with performance later in life. Competition results in dressage and show jumping for almost 40 000 horses from the beginning of the 1960s until 2006 were available. For RHQT data of 14 000 horses judged between 1988 and 2007 were used. Genetic parameters were estimated for accumulated competition results defined for different age groups (4 to 6 years of age, 4 to 9 years of age and lifetime), and for different birth year groups. Genetic correlations were estimated between results at RHQT and competitions with a multi-trait animal model. Heritabilities were higher for show jumping than dressage and increased with increasing age of the horse and amount of information. For dressage, heritabilities increased from 0.11 for the youngest group to 0.16 for lifetime results. For show jumping corresponding values increased from 0.24 to 0.28. Genetic correlations between competition results for the different age groups were highly positive (0.84 to 1.00), as were those between jumping traits at RHQT and competition results in show jumping (0.87 to 0.89). For dressage-related traits as 4-year-old and dressage competition results the estimated genetic correlations were between 0.47 and 0.77. We suggest that lifetime results from competitions should be integrated into the genetic evaluation system. However, genetic parameters showed that traits had changed during the over 35-year period covered due to the development of the sport, which needs to be considered in future genetic evaluations.

In response to environmental threats, numerous indicators have been developed to assess the impact of livestock farming systems on the environment. Some of them, notably those based on management practices have been reported to have low accuracy. This paper reports the results of a study aimed at assessing whether accuracy can be increased at a reasonable cost by mixing individual indicators into models. We focused on proxy indicators representing an alternative to the direct impact measurement on two grassland bird species, the lapwing Vanellus vanellus and the redshank Tringa totanus. Models were developed using stepwise selection procedures or Bayesian model averaging (BMA). Sensitivity, specificity, and probability of correctly ranking fields (area under the curve, AUC) were estimated for each individual indicator or model from observational data measured on 252 grazed plots during 2 years. The cost of implementation of each model was computed as a function of the number and types of input variables. Among all management indicators, 50% had an AUC lower than or equal to 0.50 and thus were not better than a random decision. Independently of the statistical procedure, models combining management indicators were always more accurate than individual indicators for lapwings only. In redshanks, models based either on BMA or some selection procedures were non-informative. Higher accuracy could be reached, for both species, with model mixing management and habitat indicators. However, this increase in accuracy was also associated with an increase in model cost. Models derived by BMA were more expensive and slightly less accurate than those derived with selection procedures. Analysing trade-offs between accuracy and cost of indicators opens promising application perspectives as time consuming and expensive indicators are likely to be of low practical utility.

A total of 24 castrated male Creole were used for digestibility studies on three sugarcane (SC) products: final molasses, SC juice and ground SC. Digestibility of macronutrients were determined in three consecutive experimental trials between 35 and 60 kg BW. The first trial measured the effect of a constant rate of incorporation of those SC products in a control diet (23% on a dry matter (DM) basis) on digestibility of energy and nutrients. The second and the third trials were designed to determine the effects of increasing rates of inclusion of SC juice and ground SC in the control diet on the digestibility of nutrients and energy. The DM content of molasses, SC juice and ground SC were 74.3%, 19.3% and 25.8%, respectively. Free sugar contents were 73.8%, 81.6% and 51.5% of DM for molasses, SC juice and ground SC, respectively. In contrast with molasses and SC juice, NDF content of ground SC was very high (40.3% DM). Energy digestibility coefficients (DC) were 99.0% and 83.6% for SC juice and molasses, respectively. For ground SC, amounts and composition of intakes differed from feed allowance as a consequence of chewing-then-spitting out most of the fibrous contents of ground SC. Expressed as a percentage of energy intake and energy allowance, DC of energy for ground SC were 68.6% and 31.9%, respectively. On an intake basis, digestible energy (DE) and metabolisable energy (ME) values were higher for SC juice (17.3 and 17.2 MJ/kg DM, respectively) than for molasses (13.1 and 12.6 MJ/kg DM, respectively) or ground SC (12.3 and 11.8 MJ/kg DM, respectively). On an allowance basis, the corresponding values for ground SC were only 5.7 and 5.6 MJ/kg DM, respectively. The gradual inclusion rate of SC juice in the diet up to 66% resulted in a linear increase of the DC of the diet organic matter and energy (P < 0.001) by 0.10% per 1% in SC juice DM. On the other hand, the partial substitution of the control diet by ground SC up to 74% resulted in a linear (P < 0.05) reduction of digestibility of nutrients and energy. Each 1% increase of ground SC incorporation rate resulted in a 0.13% and 0.61% linear decrease of DC of the energy, expressed as a percentage of energy intake or energy allowance, respectively. In conclusion, our study provides updated energy values of SC products usable as energy sources for feeding pigs.

For some time now prebiotics have been proposed to improve health by stimulation of beneficial bacteria in the intestine of humans and animals. The current study is aiming to show effects of feeding of either 2% inulin or 2% lactulose in milk replacer on performance and intestinal morphology of male Holstein–Friesian calves. After 20 weeks of feeding inulin led to significantly higher daily weight gains than lactulose while control animals ranged between the experimental feedings. Ingestion of milk replacer was reduced in lactulose treated animals. Additionally differences of villus height in jejunum (P = 0.07) and ileum (P = 0.03) could be found with an increase for lactulose treated animals and a decrease for inulin treated animals. In ileum the density of proliferative epithelial cells tended to be lower in inulin treated and higher in lactulose treated animals (P = 0.08). Both inulin and lactulose tended to decrease the quantity of goblet cells in the tips of ileal villi (P = 0.07). Both prebiotics can affect performance and intestinal morphology of calves and may as such affect animal health. But effects differ between substances.

In order to assess the relative importance of genomic imprinting for the genetic variation of traits economically relevant for pork production, a data set containing 21 209 records from Large White pigs was analysed. A total of 33 traits for growth, carcass composition and meat quality were investigated. All traits were recorded between 1997 and 2006 at a test station in Switzerland and the pedigree included 15 747 ancestors. A model with two genetic effects for each animal was applied: the first corresponds to a paternal and the second to a maternal expression pattern of imprinted genes. The imprinting variance was estimated as the sum of both corresponding genetic variances per animal minus twice the covariance. The null hypothesis of no imprinting was tested by a restricted maximum likelihood ratio test with two degrees of freedom. Genomic imprinting significantly contributed to the genetic variance of 19 traits. The proportion of the total additive genetic variance that could be attributed to genomic imprinting was of the order between 5% and 19%.

Avian embryogenesis can be manipulated by alteration of the temperature during incubation of the brooding egg. Investigations in turkeys showed that a higher temperature during early embryogenesis positively affects the myogenesis accompanied with a higher muscle fibre number (MFN). The aim of this study was to transfer this result to broiler and to investigate if an alteration of the temperature also affects the meat quality after slaughter of the birds. Therefore brooding eggs of the Cobb 500 broiler genetic were either incubated at 37.5°C during the whole incubation period (at normal temperature (NT)), or at 38.5°C during embryonic day (ED) 7 to 10 (at high temperature (HT)). After hatch the chicks were sexed and reared up to an age of 36 days in an experimental stable. Growth and feed conversion properties were determined during this period. After slaughter different meat quality characteristics as well as the muscle microstructure were analysed. The hatch rate and chick weight did not differ between the broiler of the NT and HT group. After 36 days the final body weights and the cumulative feed conversion rates were not different in the NT and HT groups. No differing results were obtained with regard to the slaughter, breast and leg weights of the NT and HT animals. Considering the gender of the animals no differences in the slaughter characteristics could be determined although the carcass and breast weights of the HT cocks were tendentially higher. The muscle fibre areas and MFNs in the breast muscles of the NT and HT cocks did not differ significantly and were in the range of the HT hens. Only the NT hens had significantly larger muscle fibres and less MFN than the other animals. With regard to the meat quality characteristics no clear differences of the pH, electrical conductivity (EC) and colour (L*a*b*) values were found. The L*a*b* values in the investigated breast muscles of all broilers usually increased during ageing. The increase of the incubation temperature had no impact on the hatch, growth, slaughter and meat quality characteristics of the broiler except for the tendentially higher carcass and breast weights of the HT cocks. However, the decrease of the fibre areas in the HT hens is an interesting effect of using a higher incubation temperature, which needs to be considered when implicating further investigations.

Miniature pigs have been recognized as valuable experimental animals in medical research. However, porcine models related to gene knockout of human diseases are not widely available. The objective of this study was to establish Mx1-Cre pigs using somatic cell nuclear transfer. In this study, we created transgenic pigs using somatic cell nuclear transfer (SCNT). Transfer of 210, 230, 250 and 215 zygotes to four surrogates produced 10 piglets. The Cre recombinase expression in transgenic pigs was studied using reverse transcriptase (RT)-PCR and immunohistochemistry. Mx1-Cre swine were shown to harbor the Cre gene in their genomic DNA using the PCR. In conclusion, Mx1-Cre transgenic piglets were successfully produced by SCNT. These transgenic swine, in conjunction with inducible systems for controlling Cre expression and function, are likely to have a profound impact on the study of human diseases.