To save content items to your account,
please confirm that you agree to abide by our usage policies.
If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account.
Find out more about saving content to .
To save content items to your Kindle, first ensure no-reply@cambridge.org
is added to your Approved Personal Document E-mail List under your Personal Document Settings
on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part
of your Kindle email address below.
Find out more about saving to your Kindle.
Note you can select to save to either the @free.kindle.com or @kindle.com variations.
‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi.
‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.
Hormone exposure in utero affects male- and female-typical behavior in animals, and these effects may persist in the next generation. Prenatal exposure to diethylstilbestrol (DES), a potent estrogen and endocrine disruptor, has been associated with a tendency toward greater heterosexual behavior in women, but the association in the next generation has not been studied. We evaluated the associations of maternal prenatal DES exposure with sexual behavior, sexual identity, and gender identity in 982 female offspring participating in the National Cancer Institute’s DES Third Generation Study, a cohort born to mothers who were prenatally exposed and unexposed to DES. Odds ratio (OR) and 95% confidence intervals (CIs) were estimated from logistic regression models that included birth year. The ORs were 0.71 (CI 0.46–1.1) for DES in relation to non-heterosexual compared with heterosexual behavior, and 0.99 (CI 0.55–1.8) for non-heterosexual identity, compared with heterosexual identity. Results were similar after additional adjustment for education. Only three individuals reported a gender identity distinct from what was reported by the mother at cohort inception, preventing meaningful quantitative analysis of DES and gender identity. These data do not provide evidence of differences in sexual behavior and sexual identity in female offspring of mothers with and without prenatal exposure to DES.
This study employs a longitudinal network approach to investigate the dynamic relationships between COVID-19-related stressors and depressive symptoms among Canadian adults and to explore any sex and age differences in these associations.
Methods
The study utilised data from the Canadian Longitudinal Study on Ageing (CLSA), a large, national, long-term study of Canadian adults aged 45 years and older. Depressive symptoms were measured using the Centre for Epidemiologic Studies Depression Scale (CES-D), and COVID-19-related stressors were evaluated using a standardised stress inventory adapted for the pandemic context. The cross-lagged panel network analysis (CLPN) was employed to examine the temporal relationships and dynamic interactions between depressive symptoms and COVID-19-related stressors.
Results
Significant variations in network structures and strengths were identified across demographic groups. Individuals aged between 45 and 65 years and females exhibited stronger connections between COVID-19-related stressors and depressive symptoms. Central symptoms such as “feeling unhappy” were consistent across groups, while “feeling depressed” was more central among males and “increased verbal or physical conflict” among females. Additionally, health-related stressors and family separation emerged as critical bridge symptoms for males and individuals under 65 years, respectively.
Conclusions
Both cross-sectional and longitudinal relationships, and directionality between COVID-19-related stressors and depressive symptoms across sex and age groups were identified. The findings of the study highlight that dedicated mental health intervention and prevention efforts are warranted to ameliorate the negative impact of stressors on depressive symptoms.
Parasite transmission can be disrupted when their free-living larval stages are consumed by non-host organisms. Yet, the contribution of benthic scrapers to this process remains insufficiently explored. Here, we experimentally assessed the ability of the North American pulmonate snail Physa acuta to reduce the abundance of free-living digenean larvae – cercariae of Diplostomum sp. and Trichobilharzia sp., and adolescariae of Notocotylus sp. – and evaluated how this effect is modulated by snail body size and colonisation by other organisms. Larval consumption by P. acuta occurred in all treatments and was highest for settled Notocotylus sp. adolescariae, particularly among larger individuals. The extent of larval reduction varied with infection by digenean metacercariae (xiphidiometacercariae), which either enhanced or inhibited feeding depending on parasite identity. It also varied with colonisation by Chaetogaster limnaei limnaei, whose presence increased the ingestion of planktonic cercariae, likely due to the combined feeding activity of the snail and its commensal oligochaete. Most snails harboured metacercariae, indicating that P. acuta frequently functions as a second intermediate host in its non-native range. Our findings highlight the dual ecological role of P. acuta – both as a consumer of free-living parasite stages and as a competent host. This trophic interaction may disrupt parasite transmission while providing nutritional benefits that support the ecological success and spread of this non-native species. Conversely, by serving as a host, P. acuta may facilitate the persistence and dissemination of parasitic taxa in invaded ecosystems.
Mental health awareness efforts are increasing, especially for ADHD. There is growing evidence that such efforts may also cause unnecessary self-diagnosis and worsening symptoms for some disorders; however, there are no validated approaches to avoid these potential harms without reducing the awareness efforts themselves. We developed a multifaceted intervention, called nocebo education. The intervention was based on the principles of the nocebo effect, where negative expectations may cause symptom misattribution and worsening. We tested whether teaching about the nocebo effect could mitigate the potential false self-diagnosis and symptom worsening from ADHD awareness.
Methods
In a double-blind randomized controlled trial with a week-long follow-up (NCT06638411), 215 healthy young adults (77% women) were randomized to participate in a group workshop on either ADHD awareness, ADHD combined with nocebo education, or control (sleep). We measured changes in self-diagnosis and ADHD symptoms immediately after the workshop (self-diagnosis), and 1-week later (self-diagnosis and symptoms).
Results
ADHD group reported substantially higher self-diagnosis scores immediately $ ({\beta}_{\mathrm{standardised}}=0.80\;\left[0.58,1.02\right],p<.001 $) and 1 week after the workshop $ (\beta =0.50\;\left[0.28,0.72\right],p<.001 $) compared to controls. These effects persisted despite no changes in reported symptoms. Nocebo education halved the false self-diagnosis scores immediately after the workshop ($ \beta =0.45\;\left[0.23,0.67\right],p<.001 $) and eliminated the false self-diagnosis entirely at follow-up $ (\beta =0.16\;\left[-0.06,0.38\right],p=.08 $).
Conclusions
We show that being exposed to ADHD awareness reliably increases false self-diagnosis among healthy young adults for at least one week; a brief nocebo education intervention is efficacious in substantially reducing and later eliminating it. Nocebo education is a promising adjunct for balanced awareness efforts that could be applied in various contexts.
Besnoitia besnoiti is an apicomplexan parasite, the causal agent of bovine besnoitiosis. This disease is characterised by cyst formation in the skin and mucosa. During early infection, fast proliferating tachyzoites invade and replicate within host endothelial cells. In non-excitable cells, extracellular signals activate the inositol-triphosphate/calcium (InsP3/Ca2+) pathway, which depends on phospholipase C (PLC) activation, inducing an increase in IP3 levels, followed by intracellular Ca2+ release. Despite the understanding of Ca2+ signalling, this process in B. besnoiti tachyzoites is unclear. This work aimed to study Ca2+ dynamics during B. besnoiti infection in bovine umbilical vein endothelial cells (BUVEC) and the role of the InsP3/Ca2+ pathway during B. besnoiti infection. Ca2+ dynamics during tachyzoite replication were determined in B. besnoiti-infected BUVEC loaded with fluo-4-AM. The role of InsP3/Ca2+ signalling for parasite invasion was evaluated by treatments with Ca2+ chelators (BAPTA, EGTA) or PLC inhibitors (U73122, D609). PLC activation was studied in fluo-4-loaded free tachyzoites using the PLC activator m-3M3FBS, in the presence or absence of PLC inhibitors. Current data show an infection-driven increase in total Ca2+ signals in B. besnoiti-infected BUVEC over time. BAPTA, but not EGTA, treatments of tachyzoites affected their invasion, reducing infection rates by 85.4 ± 9.3%, suggesting that intracellular Ca2+ sources are necessary for B. besnoiti invasion. In line, treatments with U73122 and D609 reduced infection rates by 79.3 ± 9.4% and 49.7 ± 8.9%, respectively, demonstrating that PLC participation is required for host cell infection. Finally, m-3M3FBS treatments induced a PLC-independent Ca2+ flux in B. besnoiti tachyzoites.
The genus Aphelenchoides Fischer, 1894, encompasses nearly 200 species with significant ecological and economic importance, yet its taxonomy remains complex due to morphological similarities among species and limited molecular data. In this study, we describe a new species, Aphelenchoides vinhphucensis sp. n., collected from the rhizosphere of rice (Oryza sativa L.) in Vinh Phuc Province, Vietnam. The new species is distinguished by its morphological characteristics, including a slender stylet (9.6–12.7 μm), distinct lateral fields with four lines, a conical tail with a pointed mucro, and a well-developed post-uterine sac. Males feature three pairs of caudal papillae and arcuate spicules. Scanning electron microscopy provided detailed insights into surface features, complementing light microscopy observations. Molecular analyses of the D2-D3 regions of 28S rRNA and 18S rRNA supported the distinct phylogenetic placement of Aphelenchoides vinhphucensis sp. n., differentiating it from closely related species. Bayesian phylogenetic analysis confirmed its divergence, contributing to our understanding of biodiversity of the genus Aphelenchoides. This integrative approach highlights the importance of combining classical morphological methods with modern molecular tools for accurate species identification. The discovery of Aphelenchoides vinhphucensis sp. n. underscores the nematode diversity in Vietnam, especially those associated with rice cultivation.
In LA County, contact precautions for methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus require 7.3 million gowns annually generating 506 tons of plastic waste and 1.73 million kilograms of carbon dioxide equivalents, which cause the loss of 4.07 disability-adjusted life-years. Unintended consequences of gown use necessitates exploration of infection prevention alternatives.
This study aims to provide an overview of evidence on factors affecting Mediterranean diet (MD) adherence across socio-ecological levels (individual, interpersonal and environmental) in Mediterranean countries, which can be target points for future interventions to promote MD adherence.
Design:
A systematic review was conducted following the PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) guidelines and registered in the Prospero database (CRD42020189337). Literature was searched in PubMed, Web of Science and PsycINFO.
Setting:
The MD is one of the healthiest dietary patterns, reducing risk of chronic disease while promoting better health outcomes. However, adherence to the MD remains challenging, even in Mediterranean countries.
Participants:
Healthy adults aged 18 years and older, living in a Mediterranean country.
Results:
A total of thirty-seven cross-sectional studies were included, with 190 to 13 262 participants. Most studies (30/37) were conducted in European Mediterranean countries, primarily Italy (n 14), Spain (n 9) and Greece (n 6). All studies involved community-based samples; two studies included only women. Individual-level determinants were the most frequently examined. Higher socio-economic status, regular breakfast consumption, being unemployed, a job seeker or retired were linked to better MD adherence. Socio-cognitive and interpersonal factors were underexplored. At the environmental level, COVID-19 confinement boosted adherence, whereas the effects of economic crises were inconsistent. Effect sizes were mostly very small to small, and findings are based on low-quality studies.
Conclusions:
This systematic review highlighted several socio-economic and environmental factors potentially influencing MD adherence. However, more robust research is needed to better understand socio-cognitive and ecological factors.
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a serious disease and increasingly prevalent in children. MASLD is associated with health consequences such as type 2 diabetes and CVD. While vitamin E is a potent antioxidant that has been proposed to improve liver function and cardiometabolic health including liver markers, lipid profile, glycaemic control and anthropometric measurements. A comprehensive search was conducted up to March 2025. Data on anthropometric measures, liver enzymes (alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transferase (GGT)), glycaemic indices (fasting blood sugar (FBS), insulin, homeostatic model assessment for insulin resistance (HOMA-IR)), lipid profiles (total cholesterol (TC), TAG, LDL-cholesterol, HDL-cholesterol) and serum vitamin E levels were extracted. Statistical analyses were performed using a random-effects model. Eleven randomised controlled trials involving 665 participants were included in this study. Vitamin E significantly reduced ALT (weighted mean difference (WMD) = −5·23 U/L; 95 % CI: −7·72, −2·75; P < 0·001) and AST (WMD = −3·00 U/L; 95 % CI: −4·59, −1·41; P < 0·001), reflecting improved liver function. It also decreased TC (WMD = −5·77 mg/dl; 95 % CI: −11·46, −0·09; P = 0·04) and HOMA-IR (WMD = −0·82; 95 % CI: −1·28, −0·37; P < 0·001), while significantly increasing serum vitamin E levels (WMD = 9·16 mg/l; 95 % CI: 3·29, 15·03; P = 0·002). No significant changes were observed in the BMI, GGT, FBS, insulin, LDL-cholesterol, HDL-cholesterol or TAG levels. Vitamin E supplementation in paediatric MASLD appears to favourably influence key liver enzymes such as ALT, AST and certain metabolic factors including TC, and HOMA-IR levels, supporting its potential role as adjunctive therapy.
The aim of this study was to examine the potential mediating role of intuitive eating in the relationship between food insecurity and adherence to the Mediterranean diet. A total of 1039 adults aged between 20 and 64 years living in Niğde, Türkiye, were evaluated using a questionnaire. The questionnaire included items on participants’ general characteristics, dietary habits, the Household Food Security Survey Module – Short Form, the Mediterranean Diet Adherence Screener and the Intuitive Eating Scale-2. Intuitive eating was found to play a partial mediating role in the relationship between food insecurity and adherence to the Mediterranean diet. Food insecurity was directly and negatively associated with adherence to the Mediterranean diet, and this association remained significant even after the inclusion of intuitive eating as a variable in the model. In conclusion, food insecurity was found to negatively affect adherence to the Mediterranean diet not only directly but also indirectly by weakening intuitive eating skills. Interventions aimed at promoting intuitive eating may help mitigate unhealthy dietary behaviours associated with food insecurity; however, improving food access and living conditions remains essential for a long-term solution.
To provide an up-to-date review of existing and current literature in the field of radiological and nuclear disasters to support the needs of research applications for health care and public health preparedness and response.
Methods
A systematic literature search using 4 databases to identify articles which included a multitude of topics relevant to preparedness for nuclear and radiological disasters. One hundred articles that met inclusion criteria were summarized into 7 themes addressing medical and health care preparedness for nuclear and radiological events.
Results
The review generated evidence supporting and defining various measures health care and government entities can take to improve nuclear and radiological disaster readiness and responsiveness in health systems. Strengthening preventive measures and policies, prehospital and hospital mechanisms, training and education, regional collaboration, communication, and infrastructure support were the main gaps identified.
Conclusions
An overarching concern regarding the inadequacies of the modern health care system’s radiological disaster preparedness was a clear-cut conclusion from the literature. The major challenges and proposed solutions for public safety to the growing threat of radiologic disasters were identified.
Primary care serves as the foundation of a well-functioning healthcare system and is critical for ensuring equitable access, early disease management, and cost-effective service delivery. In China, reforming primary-level healthcare institutions has become a national priority to achieve universal health coverage. Understanding the service users’ perspectives is essential to align primary care services with population health needs and improving trust in grassroots healthcare providers.
Aim:
To evaluate satisfaction and unmet service needs among primary care users in Jiangsu Province, China.
Methods:
A cross-sectional survey using structured questionnaires was given to 424 residents in urban and rural Jiangsu Province to measure satisfaction with primary hospitals, doctors, service preferences, and perceived gaps. Data was analyzed using descriptive statistics and chi-square tests to identify regional differences.
Results:
The primary care utilisation rate among respondents was 73.3%. While 75.2% reported satisfaction with medical expenses at primary-level facilities, significant urban-rural and regional differences were observed in service awareness, health policy knowledge, and perceived quality of doctor-patient communication. Primary care doctors received the highest ratings for using “easy-to-understand language”(mean score 4.13 ± 0.821) but lower scores for “professional competence” and “treatment effectiveness” (both 3.91). Rural inhabitants expressed high demand for services like management of common diseases (65.8%) and routine health examinations (52.4%), but highlighted shortages in advanced diagnostic tools (e.g., CT scans, endoscopy). Most inhabitants (67.2%) felt that primary care providers require improvement, particularly in clinical competency and communication.
Conclusion:
The findings highlight areas for targeted policy interventions to improve primary care service delivery and capacity-building of primary care doctors in Jiangsu Province.