We perform two-way coupled direct numerical simulation of particle-laden flow in an open channel at a friction Reynolds number ($Re_{\tau }$) of 5186, which exhibits many characteristics of high-Reynolds-number wall-bounded turbulence, such as the distinct separation of scales in the inner and outer layers. Three representative cases, an unladen case and low- and high-Stokes-number particle-laden cases, are performed to investigate the turbulent modification by particles. To this end, we compare several statistical quantities to understand the particle effect on momentum exchange and interphasial energy transfer. The modulation of large-scale motions (LSMs) and very-large-scale motions (VLSMs) are analysed using spectral information, and we find that the LSMs and VLSMs are generally weakened in the inner and outer layers, which is qualitatively different from similar simulations at lower Reynolds numbers ($Re_{\tau } \approx 500$). The spatial structures are investigated with correlation analysis, and inclined VLSMs are observed in the near-wall region, with decreased inclination angles by particles. The particles tend to widen and shorten the spanwise and streamwise extent of coherent structures, respectively. Furthermore, we find that the vorticity vector displays a preferential alignment with the eigenvector corresponding to the intermediate eigenvalue of the strain-rate tensor, independent of the particle Stokes number.

Experiments were conducted to study the transition and flow development in a laminar separation bubble (LSB) formed on an aerofoil. The effects of a wide range of free-stream turbulence intensity ($0.15\,\%< Tu<6.26\,\%$) and streamwise integral length scale ($4.6\ {\rm mm}<\varLambda _{u}<17.2\ {\rm mm}$) are considered. The co-existence of modal instability due to the LSB and non-modal instability caused by streaks generated by free-stream turbulence is observed. The flow field is measured using hot-wire anemometry, which showed that the presence of streaks in the boundary layer modifies the mean-flow topology of the bubble. These changes in the mean flow field result in the modification of the convective disturbance growth, where an increase in turbulence intensity is found to dampen the growth of the modal instability. For a relatively fixed level of $Tu$, the variation of $\varLambda _{u}$ has modest effects. However, a slight advancement of the nonlinear growth of disturbances and eventual breakdown with the decrease in $\varLambda _{u}$ is observed. The data show that the streamwise growth of the disturbance energy is exponential for the lowest levels of free-stream turbulence and gradually becomes algebraic as the level of free-stream turbulence increases. Once a critical turbulence intensity is reached, there is enough energy in the boundary layer to suppress the laminar separation bubble, resulting in the non-modal instability taking over the transition process. Linear stability analysis is conducted in the fore position of the LSB. It accurately models incipient disturbance growth, unstable frequencies and eigenfunctions for configurations subjected to turbulence intensity levels up to 3 %, showing that the mean-flow modification due to the non-modal instability dampens the modal instability.