The effects of supplements of protein meal or sorghum grain given to young cattle for 140 days during their first winter and spring post-weaning, were recorded over 560 days for cattle grazing on low quality pastures in subtropical Australia. The supplements were offered to steers in four treatment groups, replicated three times, at the following rates per head to late spring: (a) mineral mix (M) + cotton-seed hulls, 90g/day (control), (b) M + protein meal, 600 g/day (protein daily), (c) as for (b) but at 2·1 kg/3·5 days (protein twice-weekly), or (d) M + sorghum grain, 560 g/day (sorghum). For the next 130 days, to late summer, only supplement (c) was continued while the remaining three groups were not supplemented. None of the supplements was offered for the final 290 days of the experiment when the steers grazed an improved pasture.

At the end of the first spring, the live weight of the steers supplemented daily and twice-weekly with protein was respectively 229 and 216 kg. These were significantly (p < 0·01) heavier than the sorghum-supplemented (174 kg) or control steers (160 kg). At the end of the first summer, the steers given supplements of protein meal twiceweekly, or daily, remained significantly heavier (265, 250 kg respectively) than sorghum-supplemented or control steers (209, 198 kg). By the second summer, the steers previously given protein supplements were still heavier than the steers from the control group (357 v. 317 kg, respectively).

In steers which were offered the protein supplement during winter and spring, samples of blood taken at the end of spring had lower (P < 0·01) plasma ratios of glycine to branched-chain amino acids (valine, leucine and isoleucine) and a lower ratio of methionine: valine, than in the steers which were not offered the supplement. These ratios were significantly (P < 0·05) correlated with live-weight change over the preceding 140 days. On this basis it was considered that the improved growth of the steers offered the protein supplements was associated with an increased availability of specific essential amino acids, which may have initiated in the steers an increased appetite for pasture D.M.

Following the measurement of tritiated water (TOH) spaces, 31 cattle were slaughtered and chemically analysed in this study. They included several breeds, both females and castrate males, and were of varied nutritional history. Their body-fat content ranged from 4 to 21% of fasted live weight.

Total body water (including the water in the gut contents) was reliably estimated from TOH space, measured after allowing an overnight 16 h waterless fast for TOH equilibration. Following this regime, residual D.M. in the gut contents amounted to 1·75% of fasted live weight. The relationships of body fat to body weight, and body fat to body water when both were expressed as percentages of body weight, were too variable to be used in any predictive fashion. Equations were derived, using fasted live weight, allowing the accurate estimation in vivo of the quantities of the chemical components in the whole body (i.e. total body minus D.M. in gut contents).

It was demonstrated that the sum of total body water and total body fat constituted virtually 80% of total body tissues, and that total body protein closely approximated 80% of the fat-free dry matter, in cattle varying widely in body condition. These relationships constitute the physiological basis of the equations presented.

Comparable principles appear to apply to sheep, and a range of other mammalian species.

In a study with chicks, the metabolizable energy (ME) values of two maize and two wheat offal samples were estimated using regression procedures in which the ME of the fishmeal supplement, common to all diets, was first calculated.

From chemical analysis, the wheat offal samples would have been expected to yield at least as much ME as oats, but results fell short of expectation by as much as a quarter in the case of one sample of wheat middlings. The significance of these findings is discussed.

By comparison with previously determined ME values on the same samples fed to sheep, the chicken tended to metabolize more of the maize energy and less of the wheat offal energy.

The wool-severing properties of 2·8% aqueous solution of calcium thioglycollate were increased by 76% by dissolving the depilatory agent in a KCl-NaOH buffer of pH 12·5. The optimum Ca-thioglycollate concentration for defleecing was 3·6%. One ml of this solution defleeced an area of 12·1 cma.

A comparison of five buffers and water as solvents for 3·6% Ca-thioglycollate indicated that the area of skin defleeced was directly related to solution pH and the stubble remaining appeared to be inversely related to solution pH. A KCl-NaOH buffer of pH 12·0 provided the optimum relationship between area defleeced (10·2 cm2/ml applied) and stubble (ca. 2 mm wool) remaining.

Single site applications of 0·1–1·0 ml of defleecing agent at skin level were compared and it was shown that the application of 0·1 ml aliquots would require 320 ml solution to defleece an average-sized sheep.

Preliminary evaluation of 6% formaldehyde buffered to pH 6 and a carbowax-citric acid solution of pH 2 showed that protection of fibres against dissolution by Cathioglycollate solutions can be sustained for 21 days. Pre-treatment with these compounds could therefore produce a protected band of wool immediately above the proposed site of wool dissolution.

The practical implications of these findings are discussed with regard to a wool harvesting programme.

The forage potential of Andropogon gayanus (gamba grass) was assessed in cutting experiments on a low fertility, acid Ultisol in Colombia. This grass recently introduced from Africa outyielded Hyparrhenia rufa, Panicum maximum, Brachiaria decumbens and Digitaria decumbena in various mixtures made up of a single grass and a single legume. A. gayanua performed well as a companion grass with the legumes Styloaanthes guianensis, Stylosanthes capitata and Centroaema sp., while B. decumbena suppressed the legumes planted in these trials during the 2nd year following establishment.

Milk production of cows grazing Rhodes grass without the addition of grain supplements can be increased by feeding casein protected from deamination in the rumen with formaldehyde (Stobbs, Minson & McLeod 1977; Flores, Stobbs & Minson, 1979). No increase in milk production was achieved when unprotected casein was fed, indicating that production on a tropical grass was limited by the quantity of amino acids absorbed from the small intestines (Stobbs et al. 1977). Formaldehydetreated casein (formal-casein) increased by 5% the milk production of cows grazing ryegrass in New Zealand when an energy supplement was also fed but had no effect oh milk yield when only ryegrass was fed (Wilson, 1970). In a review of this subject Clark (1975) reported that pen studies showed post-ruminal supplementation with casein increased milk production from 1 to 4 kg/day. The result with ryegrass in the absence of an energy supplement is therefore unexpected and warrants further study.

It is now possible to synchronize the cycle of ewes at all times of the year (Gordon, 1975). Conception rates approaching those obtained following natural service have been reported following double (Colas & Brice, 1970; Colas et al. 1973; Barlow, Pryce-Jones & Reed, 1974; Gordon, 1975) or single (Smith, Boland & Gordon, 1978) set-time inseminations. In France, synchronization of ewes' oestrous cycle is based on the use of a Cronolone pessary (Colas, 1975), while field trials conducted in Britain have used the 60 mg Medroxy progesterone acetate (MAP) sponge (Barlow et al. 1974). Robinson et al. (1967) in an evaluation of different progestagen treatments found MAP to be less predictable than Cronolone. The purpose of the present experiment therefore was to evaluate the use of a 30 mg Cronolone or a 60 mg MAP intravaginal treatment when used in conjunction with fixed-time inseminations. In addition, two dose levels of pregnant mare serum gonadotrophin (PMSG) were compared.

The addition of superphosphate increases the legume content of grass-legume pastures and the growth rate of beef cattle (Shaw, 1978). It has generally been assumed that the increase in beef production following the application of superphosphate is related directly to greater amounts of legume in the pasture consumed (Norman, 1970; Shaw, 1978) but there is no direct evidence on this subject. This paper reports a study of the influence of small quantities of superphosphate on preference for legume by cattle grazing Stylosanthes-native grass pastures.

Estimates of the degradability of protein in the rumen are essential for the application of new systems which have been suggested for the evaluation of the protein requirements of ruminants (Agricultural Research Council, 1980). The effective percentage degradability (P) of protein supplements in the rumen is dependent not only on the course of degradation of the protein particles in the rumen, but also on the time distribution of their stay in the rumen, and will decrease if there is an increase in the rate of passage of the particles. It was shown by Ørskov & McDonald (1979) that if the percentage protein disappearance (p) from samples incubated for time t is described by the equation and if k is the fractional rate of passage from the rumen, then the effective degradability can be calculated as . The calculation depends on the equation for p remaining valid from t =0 (time of ingestion) until a time when all the particles have passed beyond the rumen. It has been found that for some protein supplements, linseed meal for example, the equation for p does not hold true for low values of t. The present note proposes a modification to the formula for effective degradability so that it may remain valid under these circumstances.

Research has shown that it is now possible to control the oestrous cycle of ewes with a high degree of precision (Robinson et al. 1967; Gordon, 1975; Boland, Kelleher & Gordon, 1978). There is general agreement that a high concentration of progestagen, followed by rapid withdrawal and adequate ovarian stimulation is necessary for acceptable fertility (Robinson et al. 1967; Gordon, 1975; Colas, 1975). Application of artificial insemination in France has involved the use of a 40–45 mg Cronolone sponge (Cognie, Mariana & Thimonier, 1970; Colas et al. 1973) while controlled breeding in sheep in Ireland has been used in conjunction with a 30 mg Cronolone pessary (Gordon, 1975; Smith, Boland & Gordon, 1978, 1981). The present experiment was designed to compare the relative effectiveness of two doses of Cronolone (30 or 45 mg) when administered in conjunction with two quantities(375 or 750 i.u.) of pregnant mare serum gonadotrophin (PMSG), given by intramuscular injection.

Several plants are cultivated for medicinal and aromatic purposes. Foxglove (Digitalis lanata) has been introduced into India from Western Europe and is cultivated for medicinal use. The leaves of D. lanata contain various glycosides, namely digoxin, gitoxin and gitalin, which are of medicinal value. This crop is cultivated throughout the year but not much information is available about

seasonal influence on the glycoside content. Hence, attempts are made to assess the effect of seasonal variation on total glycoside content and to arrive at an optimum season for harvesting the crop.