Hostname: page-component-5d59c44645-dknvm Total loading time: 0 Render date: 2024-03-01T13:43:46.246Z Has data issue: false hasContentIssue false

A contribution to the taxonomy of the genus Rinodina (Physciaceae, lichenized Ascomycotina) using combined ITS and mtSSU rDNA data

Published online by Cambridge University Press:  03 August 2010

M. G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereschenkivs'ka 2, 01601 Kyiv, Ukraine. Email:
Martin GRUBE
Institute of Plant Sciences, Karl-Franzens-Universität Graz, Holteigasse 6, A-8010 Graz, Austria.
Institute of Plant Sciences, Karl-Franzens-Universität Graz, Holteigasse 6, A-8010 Graz, Austria.


To test the phylogenetic position of phenotypically peculiar species in the Physciaceae we generated 47 new sequences (26 of nrITS region and 21 of mtSSU rDNA) from 19 crustose taxa of Physciaceae mainly from the genus Rinodina. Phylogenetic analysis confirmed the Buellia and Physcia groups. The analysis revealed a considerable variability of characters traditionally used for classification, especially in the delimitation of the genera Buellia and Rinodina. While ascus types agree well with the distinction of the Buellia and Physcia groups, none of the other traditional characters, including excipulum type and ascospore thickening, were consistent within subclades of the Physcia group. We suggest that both excipulum type and ascospore characters are rather dynamic in the evolution of Rinodina species and only appear consistent in morphologically more complex foliose and fruticose groups, which are characterized by thallus characters not present in the crustose groups. Two recent taxonomic changes are supported by molecular characters: Endohyalina insularis (syn. ‘Rinodina’ insularis) and Rinodina lindingeri (syn. ‘Buellia’ lindingeri). In addition Rinodina parvula (syn. ‘Buellia’ parvula) is reinstated. New records for Endohyalina brandii, E. diederichii, E. insularis and Rinodina albana are presented.

Research Article
Copyright © British Lichen Society 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)


Arnold, F. (1896) Lichenologische Ausflüge in Tirol. XXVIII. Wolkenstein. Verhandlungen der Kaiserlich-Königlichen Zoologisch-Botanischen Gesellschaft in Wien 46: 112122.Google Scholar
Calatayud, V., Atienza, V. & Barreno, E. (1995) Lichenicolous fungi from the Iberian Peninsula and the Canary Islands. Mycotaxon 55: 363382.Google Scholar
Crespo, A., Blanco, O. & Hawksworth, D. L. (2001) The potential of mitochondrial DNA for establishing phylogeny and stabilising generic concepts in the parmelioid lichens. Taxon 50: 807819.Google Scholar
Cubero, O. F., Crespo, A., Fatehi, J. & Bridge, P. D. (1999) DNA extraction and PCR amplificationmethod suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Systematics and Evolution. 217: 243249.Google Scholar
Erichsen, C. F. E. (1926) Beiträge zur Lichenenflora von Teneriffa. Hedwigia 66: 275282.Google Scholar
Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes – application for the identification of mycorrhizae and rusts. Molecular Ecology 2: 113118.Google Scholar
Giavarini, V., James, P. W. & Purvis, O. W. (2009) Rinodina. In The Lichens of Great Britain and Ireland (Smith, C. W., Aptroot, A., Coppins, B. J., Fletcher, A., Gilbert, O. L., James, P. W. & Wolseley, P. A., eds): 812825. London: British Lichen Society.Google Scholar
Giralt, M. (2001) The lichen genera Rinodina and Rinodinella (lichenized Ascomycetes, Physciaceae) in the Iberian Peninsula. Bibliotheca Lichenologica 79: 1160.Google Scholar
Giralt, M. & Matzer, M. (1994) The corticolous species of the genus Rinodina with biatorine and lecideine apothecia in southern Europe and Macaronesia. Lichenologist 26: 319332.Google Scholar
Giralt, M. & Mayrhofer, H. (1994) Four corticolous species of the genus Rinodina (lichenized Ascomycetes, Physciaceae) containing atranorin in southern Europe and adjacent regions. Nova Hedwigia 59: 129142.Google Scholar
Giralt, M. & Mayrhofer, H. (1995) Some corticolous and lignicolous species of the genus Rinodina (lichenized Ascomycetes, Physciaceae) lacking secondary lichen compounds and vegetative propagules in southern Europe and adjacent regions. Bibliotheca Lichenologica 57: 127160.Google Scholar
Giralt, M., van den Boom, P. P. G. & Elix, J. A. (2010a) ‘Buellia’ lindingeri and Rinodina hallii (Physciaceae), two closely related species. Bryologist 113: 99105.Google Scholar
Giralt, M., van den Boom, P. P. G. & Elix, J. A. (2010b) Endohyalina, the genus in the Physciaceae to accommodate the species of the Rinodina ericina-group. Mycological Progress 9: 3748.Google Scholar
Grube, M. & Arup, U. (2001) Molecular and morphological evolution in the Physciaceae (Lecanorales, lichenized Ascomycotina), with special emphasis on the genus Rinodina. Lichenologist 33: 6372.Google Scholar
Hafellner, J. (1979) Karschia Revision einer Sammelgattung an der Grenze von lichenisierten und nicht lichenisierten Ascomyceten. Beihefte zur Nova Hedwigia 62: 1248.Google Scholar
Hafellner, J. (1995) Bemerkenswerte Funde von Flechten und lichenicolen Pilzen auf makaronesischen Inseln III. Einige bisher auf den Kanarischen Inseln übersehene lecanorale Arten. Linzer Biologische Beiträge 27: 489505.Google Scholar
Hafellner, J. & Mayrhofer, H. (2007) A contribution to the knowledge of lichenicolous fungi and lichens occurring in New Zealand. Bibliotheca Lichenologica 95: 225266.Google Scholar
Hafellner, J., Mayrhofer, H & Poelt, J. (1979) Die Gattungen der Flechtenfamilie Physciaceae. Herzogia 5: 3979.Google Scholar
Hafellner, J., Petutschnig, W., Taurer-Zeiner, C. & Mayrhofer, H. (2005) Zur Flechtendiversität in den Gurktaler Alpen (Österreich: Kärnten, Steiermark und Salzburg). Herzogia 18: 79138.Google Scholar
Halici, M. G., John, V. & Aksoy, A. (2005) Lichens of Erciyes Mountain (Kayseri, Turkey). Flora Mediterranea 15: 567580.Google Scholar
Helms, G., Friedl, T. & Rambold, G. (2003) Phylogenetic relationships of the Physciaceae inferred from rDNA sequence data and selected phenotypic characters. Mycologia 95: 10781099.Google Scholar
Hitch, C. (2006) New, rare and interesting lichens. British Lichen Society Bulletin 99: 3446.Google Scholar
Hofmann, P., Wittmann, H., Türk, R. & Breuss, O. (1993) Die Flechten und Flechtenparasiten von Osttirol (Österreich) – ein erster Überblick. Herzogia 9: 837879.Google Scholar
Huelsenbeck, J. P. & Ronquist, F. (2001) MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754755.Google Scholar
Kaschik, M. (2006) Taxonomic studies on saxicolous species of the genus Rinodina (lichenized Ascomycetes, Physciaceae) in the southern hemisphere with emphasis in Australia and New Zealand. Bibliotheca Lichenologica 93: 1162.Google Scholar
Kirk, P. M., Cannon, P. F., Minter, D. W. & Stalpers, J. A., (Eds) (2008) Ainsworth & Bisby's Dictionary of the Fungi, 10th edn. Wallingford, UK: CABI International.Google Scholar
Kotlov, Yu. V. (2008) Rinodina (Ach.) Gray. In Handbook of the Lichens of Russia, Volume 10 (Golubkova, N. S., ed.): 309360. St. Petersburg: Nauka (In Russian).Google Scholar
Marbach, B. (2000) Corticole und lignicole Arten der Flechtengattung Buellia sensu lato in den Subtropen und Tropen. Bibliotheca Lichenologica 74: 1384.Google Scholar
Matzer, M. & Mayrhofer, H. (1994) The saxicolous Rinodina teichophila and three closely related species from the southern hemisphere (Physciaceae, lichenized Ascomycetes). Acta Botanica Fennica 150: 109120.Google Scholar
Matzer, M. & Mayrhofer, H. (1996) Saxicolous species of the genus Rinodina (lichenized Ascomycetes, Physciaceae) in southern Africa. Bothalia 26: 1130.Google Scholar
Matzer, M., Mayrhofer, H. & Elix, J. A. (1998) Rinodina peloleuca (Physciaceae), a maritime lichen with a distinctive austral distribution. New Zealand Journal of Botany 36: 175188.Google Scholar
Mayrhofer, H. (1982) Ascosporen und die Evolution der Flechtenfamilie Physciaceae. Journal of the Hattori Botanical Laboratory 52: 313321.Google Scholar
Mayrhofer, H. (1983) The saxicolous species of Rinodina in New Zealand. Lichenologist 15: 267282.Google Scholar
Mayrhofer, H. (1984a) Die saxicolen Arten der Flechtengattung Rinodina und Rinodinella in der Alten Welt. Journal of the Hattori Botanical Laboratory 55: 327493.Google Scholar
Mayrhofer, H. (1984b) The saxicolous species of Dimelaena, Rinodina and Rinodinella in Australia. Beihefte zur Nova Hedwigia 79: 511536.Google Scholar
Mayrhofer, H. & Lambauer, M. (2004) Additional lichen records from New Zealand 41. Saxicolous and lichenicolous species of the genus Rinodina. Australasian Lichenology 54: 2832.Google Scholar
Mayrhofer, H. & Moberg, R. (2002) Rinodina. Nordic Lichen Flora 2: 4169, 7273, 8287 and 100115.Google Scholar
Mayrhofer, H. & Poelt, J. (1979) Die saxicolen Arten der Flechtengattung Rinodina in Europa. Bibliotheca Lichenologica 12: 1186.Google Scholar
Mayrhofer, H. & Sheard, J. W. (2007) Rinodina archaea (Physciaceae, lichenized Ascomycetes) and related species. Bibliotheca Lichenologica 96: 229246.Google Scholar
Mayrhofer, H., Scheidegger, C. & Sheard, J. W. (1990) Rinodina lecanorina and Rinodina luridata, two closely related species on calciferous rocks. Bibliotheca Lichenologica 38: 335356.Google Scholar
Mayrhofer, H., Sheard, J. W., Grassler, M. C. & Elix, J. A. (2001) Rinodina intermedia Bagl. (Physciaceae, lichenized Ascomycetes): a well characterized species with submuriform ascospores. Bryologist 104: 456463.Google Scholar
Molina, M. C., Crespo, A., Blanco, O., Hladun, N. & Hawksworth, D. L. (2002) Molecular phylogeny and status of Diploicia and Diplotomma, with observations on Diploicia subcanescens and Diplotomma rivas-martinezii. Lichenologist 34: 509519.Google Scholar
Myllys, L., Tehler, A. & Lohtander, K. (2001) ß-tubulin, ITS and group I intron sequences challenge the species pair concept in Physcia aipolia and P. caesia. Mycologia 93: 335343.Google Scholar
Navarro-Rosinés, P. & Hladun, N. L. (1990) Flora liquénica de las rocas carbonatadas del Valle de Núria (Pirineos, Cataluña). Actas del II Coloquio Internacional de Botánica Pirenaico-Cantábrica. Monografias del Instituto Pirenaico de Ecología 5: 7583.Google Scholar
Orange, A., James, P. W. & White, F. J. (2001) Microchemical Methods for the Identification of Lichens. London: British Lichen Society.Google Scholar
Page, R. D. M. (1996) TreeView: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12(4): 357358.Google Scholar
Poelt, J. & Mayrhofer, H. (1979) Studien über Ascosporen-Typen der Flechtengattung Rinodina. Beihefte zur Sydowia 8: 312331.Google Scholar
Rambold, G., Mayrhofer, H. & Matzer, M. (1994) On the ascus types in the Physciaceae (Lecanorales). Plant Systematics and Evolution 192: 3140.Google Scholar
Ropin, K. & Mayrhofer, H. (1993) Zur Kenntnis corticoler Arten der Flechtengattung Rinodina in den Ostalpen und angrenzenden Gebieten. Herzogia 9: 779835.Google Scholar
Ropin, K. & Mayrhofer, H. (1995) Über corticole Arten der Gattung Rinodina (Physciaceae) mit grauem Epihymenium. Bibliotheca Lichenologica 58: 361382.Google Scholar
Scheidegger, C. (1993) A revision of European saxicolous species of the genus Buellia de Not. and formerly included genera. Lichenologist 25: 315364.Google Scholar
Sheard, J. W. (2004) Rinodina. In Lichen Flora of the Greater Sonoran Desert Region, Volume 2 (Nash, T. H. III, Ryan, B. D., Diederich, P., Gries, C. & Bungartz, F., eds): 467502. Tempe, Arizona: Lichens Unlimited, Arizona State University.Google Scholar
Sheard, J. W. & Mayrhofer, H. (2002) New species of Rinodina (Physciaceae, lichenized Ascomycetes) from western North America. Bryologist 105: 645672.Google Scholar
Swofford, D. L. (2002) PAUP. Phylogenetic Analysis Using Parsimony. Version 4.0. Sunderland, Mass.: Sinauer Associates.Google Scholar
Triebel, D., Rambold, G. & Nash, T. H. III (1991) On lichenicolous fungi from continental North America. Mycotaxon 42: 263296.Google Scholar
Wedin, M., Baloch, E. & Grube, M. (2002) Parsimony analyses of mtSSU and nrITS sequences reveal the natural relationships of the lichen families Physciaceae and Caliciaceae. Taxon 51: 655660.Google Scholar
White, T. J., Bruns, T. D., Lee, S., Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal DNA genes for phylogenies. In PCR Protocols: A Guide to Methods and Applications (Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J., eds): 315322. San Diego: Academic Press.Google Scholar
Wirth, V. (1995) Flechtenflora, 2. Auflage. Stuttgart: Ulmer.Google Scholar
Zahlbruckner, A. (1926) Lichenes (Flechten). B. Spezieller Teil. In Die natürlichen Pflanzenfamilien, Band. 8, 2.Auflage. (Engler, A., ed.): 61270. Leipzig: Engelmann Verlag.Google Scholar
Zoller, S., Scheidegger, C. & Sperisen, C. (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming Ascomycetes. Lichenologist 31: 511516.Google Scholar