Skip to main content
    • Aa
    • Aa

ALS-Resistant Smallflower Umbrella Sedge (Cyperus difformis) in Arkansas Rice: Physiological and Molecular Basis of Resistance

  • Parsa Tehranchian (a1), Dilpreet S. Riar (a1), Jason K. Norsworthy (a1), Vijay Nandula (a2), Scott McElroy (a3), Shu Chen (a3) and Robert C. Scott (a4)...

Smallflower umbrella sedge is a problematic weed in direct-seeded rice in the midsouthern United States. It recently has evolved resistance to the acetolactate synthase (ALS) –inhibiting herbicide halosulfuron in Arkansas rice. Studies were conducted (1) to determine if the resistant biotype is cross resistant to other ALS-inhibiting herbicides, (2) to evaluate alternative herbicide control options, and (3) to determine the mechanism of resistance. Whole-plant bioassay revealed that halosulfuron-resistant plants were not controlled by bispyribac–sodium, imazamox, and penoxsulam at the labeled field rate of each herbicide. The level of resistance to these herbicides, based on the lethal dose needed to kill 50% of plants (LD50) was ≥ 15-fold compared to a susceptible biotype. Both biotypes were controlled >96% with bentazon and propanil and ≤ 23% with quinclorac, thiobencarb, and 2,4-D. Hence, effective control measures exist; albeit, the number of herbicide options appear limited. Based on in vitro ALS enzyme assays, altered target site is the mechanism of resistance to halosulfuron and imazamox. Massively parallel sequencing with the use of the Illumina HiSeq detected an amino acid substitution of Pro197-to-His in the resistant biotype that is consistent with ALS-inhibiting herbicide resistance in other weed species.

Corresponding author
Corresponding author's E-mail:
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

A Bräutigam , U Gowik (2010) What can next generation sequencing do for you? Next generation sequencing as a valuable tool in plant research. Plant Biol. 12:831841

L Galon , LE Panozzo , JA Noldin , G Concenço , CP Tarouco , EA Ferreira , D Agostinetto , AA Silva , FA Ferreira (2008) Herbicide resistance of Cyperus difformis to ALS-inhibitors in paddy rice of Santa Catarina. Planta Daninha. 26:419427

BJ Haas , et al. 2013. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nature Prot. 8:14941512

YI Kuk , KH Kim , OD Kwon , DJ Lee , NR Burgos , S Jung , JO Guh (2004) Cross-resistance pattern and alternative herbicides for Cyperus difformis resistant to sulfonylurea herbicides in Korea. Pest Manag Sci. 60:8594

H McWilliam , W Li , M Uludag , S Squizzato , YM Park , N Buso , AP Cowley , R Lopez (2013) Analysis tools web services from the EMBL-EBI. Nucl Acids Res. 41:W597W600

A Merotto Jr., M Jasieniuk , MD Osuna , F Vidotto , A Ferrero , AJ Fischer (2009) Cross-resistance to herbicides of five ALS-inhibiting groups and sequencing of the ALS gene in Cyperus difformis L. J Agric Food Chem. 57:13891398

VK Nandula , CG Messersmith (2000) Mechanism of wild oat (Avena fatua L.) resistance to imazamethabenz-methyl. Pestic Biochem Physiol. 68:148155

MD Osuna , F Vidotto , AJ Fischer , DE Bayer , R De Prado , A Ferrero (2002) Cross resistance to bispyribac–sodium and bensulfuron-methyl in Echinochloa phyllopogon and Cyperus difformis . Pestic Biochem Physiol. 73:917

SB Powles , Q Yu (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol. 61:317347

DS Riar , JK Norsworthy , JA Bond , MT Bararpour , MJ Wilson , RC Scott (2012) Resistance of Echinochloa crus-galli populations to acetolactate synthase-inhibiting herbicides. Int J Agron. 2012:893953

DS Riar , JK Norsworthy , V Srivastava , V Nandula , JA Bond , RC Scott (2013) Physiological and molecular basis of acetolactate synthase-inhibiting herbicide resistance in barnyardgrass (Echinochloa crus-galli). J Agric Food Chem. 61:278289

B Siminszky (2006) Plant cytochrome P450-mediated herbicide metabolism. Phytochem Rev. 5:445458

PJ Tranel , TR Wright (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci. 50:700712

H Yasuor , MD Osuna , A Ortiz , NE Saldaín , JW Eckert , AJ Fischer (2009) Mechanism of resistance to penoxsulam in late watergrass [Echinochloa phyllopogon (Stapf) Koss.]. Agric Food Chem. 57:36533660

JS Yuan , PJ Tranel , CN. Stewart Jr. (2007) Non-target-site herbicide resistance: a family business. Trends Plant Sci. 12:613

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Weed Science
  • ISSN: 0043-1745
  • EISSN: 1550-2759
  • URL: /core/journals/weed-science
Please enter your name
Please enter a valid email address
Who would you like to send this to? *



Full text views

Total number of HTML views: 0
Total number of PDF views: 10 *
Loading metrics...

Abstract views

Total abstract views: 72 *
Loading metrics...

* Views captured on Cambridge Core between 20th January 2017 - 22nd September 2017. This data will be updated every 24 hours.