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A Pro106 to Ala Substitution is Associated with Resistance to Glyphosate in Annual Bluegrass (Poa annua)

  • Robert B. Cross (a1), Lambert B. McCarty (a1), Nishanth Tharayil (a1), J. Scott McElroy (a2), Shu Chen (a2), Patrick E. McCullough (a3), Brian A. Powell (a4) and William C. Bridges (a5)...
Abstract

Glyphosate is used in the transition zone to control annual bluegrass in fully dormant warm-season grasses. A suspected resistant (R) biotype of annual bluegrass was identified on a golf course in South Carolina after at least 10 consecutive years of glyphosate application. Greenhouse bioassays revealed the R biotype was 4.4-fold resistant to glyphosate compared with a standard susceptible (S) biotype. Further studies were conducted to investigate the mechanism conferring glyphosate resistance in the R biotype. Leaf discs of both biotypes accumulated shikimate in response to increasing glyphosate concentration, but the glyphosate concentration resulting in 50% EPSP synthase inhibition as a result of shikimate accumulation (I 50) was 4.2-fold higher in the R biotype compared with the S biotype. At the whole plant level, similar levels of shikimate accumulation were observed between biotypes at 6 and 24 h after treatment (HAT) with glyphosate, but greater shikimate accumulation occurred in the S biotype at 72, 120, and 168 HAT. Shikimate levels decreased in the R biotype after 72 HAT. There were no differences in 14C-glyphosate absorption between biotypes. However, more 14C-glyphosate translocated out of the treated leaf in the R biotype and into root tissues over time compared with the S biotype. Partial sequencing of the EPSP synthase gene revealed a point mutation that resulted in an Ala substitution at Pro106. Although other mechanisms may contribute to glyphosate resistance, these results confirm a Pro106 to Ala substitution is associated with resistance to glyphosate in the R annual bluegrass biotype.

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Corresponding author
Corresponding author's E-mail: bmccrty@clemson.edu
References
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Alarcón-Reverte R, Garcia A, Urzua J, Fischer AJ (2013) Resistance to glyphosate in junglerice (Echinochloa colona) from California. Weed Sci. 61:4854
Baerson SR, Rodriguez DJ, Tran M, Feng Y, Biest NA, Dill GM (2002) Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:12651275
Binkholder KM, Fresenburg BS, Teuton TC, Xiong X, Smeda RJ (2011) Selection of glyphosate-resistant annual bluegrass (Poa annua) on a golf course. Weed Sci. 59:286289
Bostamam Y, Malone JM, Dolman FC, Boutsalis P, Preston C (2012) Rigid ryegrass (Lolium rigidum) populations containing a target site mutation in EPSPS and reduced glyphosate translocation are more resistant to glyphosate. Weed Sci. 60:474479
Bradshaw LD, Padgette SR, Kimball SL, Wells BH (1997) Perspectives on glyphosate resistance. Weed Technol. 11:189198
Brosnan JT, Breeden JK, Mueller TC (2012) A glyphosate-resistant biotype of annual bluegrass in Tennessee. Weed Sci. 60:97100
Collavo A, Sattin M (2012) Resistance to glyphosate in Lolium rigidum selected in Italian perennial crops: bioevaluation, management and molecular bases of target-site resistance. Weed Res. 52:1624
Coupland D, Caseley JC (1979) Presence of 14C activity in root exudates and guttation fluid from Agropyron repens treated with 14C-labeled glyphosate. New Phytol. 83:1722
Cromartie TH, Polge ND (2000) An improved assay for shikimic acid and its use as a monitor for the activity of sulfosate. Proc Weed Sci Soc Am. 40:291
Darmency H, Gasquez J (1983) Esterase polymorphism and growth form differentiation in Poa annua L. New Phytol. 95:289297
Ellis WM (1973) The breeding system and variation in populations of Poa annua L. Evolution. 27:656662
Eschenburg S, Healy ML, Priestman MA, Lushington GH, Schönbrunn E (2002) How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli . Planta. 216:129135
Feng PCC, Tran M, Chiu T, Sammons RD, Heck GR, CaJacob CA (2004) Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci. 52:498505
Franz JE, Mao JA, Sikorski JA (1997) Glyphosate: A Unique Global Herbicide. Washington, DC American Chemical Society. 653 p
Funke T, Yang Y, Han H, Healy-Fried M, Olesen S, Becker A, Schönbrunn E (2009) Structural basis of glyphosate resistance resulting from the double mutation Thr197 → Ile and Pro101 → Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli . J Biol Chem. 284:98549860
Gaines TA, Zhang W, Wang D, Bukun B, Chisholm ST, Shaner DL, Nissen SJ, Patzoldt WL, Tranel PJ, Culpepper AS, Grey TL, Webster TM, Vencill WK, Sammons RD, Jiang J, Preston C, Leach JE, Westra P (2010) Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc Natl Acad Sci USA. 107:10291034
Ge X, d'Avignon DA, Ackerman JJH, Sammons RD (2010) Rapid vacuolar sequestration: the horseweed glyphosate resistance mechanism. Pest Manag Sci. 66:345348
Geiger DR, Bestman HD (1990) Self-limitation of herbicide mobility by phytotoxic action. Weed Sci. 38:324329
González-Torralva F, Gil-Humanes J, Barro F, Brants I, De Prado R (2012a) Target site mutation and reduced translocation are present in a glyphosate-resistant Lolium rigidum Lam. biotype from Spain. Plant Physiol Biochem. 58:1622
González-Torralva F, Rojano-Delgado AM, de Castro MDL, Mülleder N, De Prado R (2012b) Two non-target mechanisms are involved in glyphosate-resistant horseweed (Conzya Canadensis L. Cronq.) biotypes. J Plant Physiol. 169:16731679
Healy-Fried ML, Funke T, Priestman MA, Han H, Schönbrunn E (2007) Structural basis of glyphosate tolerance resulting from mutations of Pro101 in Escherichia coli 5-enolpyruvylshikimate-3-phosate synthase. J Biol Chem. 282:32,94932,955
Heap I (2014) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.com. Accessed October 7, 2014
Herrmann KM, Weaver LM (1999) The shikimate pathway. Ann Rev Plant Physiol Plant Mol Biol. 50:473503
Jasieniuk M, Ahmad R, Sherwood AM, Firestone JL, Perez-Jones A, Lanini WT, Mallory-Smith C, Stednick Z (2008) Glyphosate-resistant Italian ryegrass (Lolium multiflorum) in California: distribution, response to glyphosate, and molecular evidence for an altered target enzyme. Weed Sci. 56:496502
Kaundun SS, Zelaya IA, Dale RP, Lycett AJ, Carter P, Sharples KR, McIndoe E (2008) Importance of the P106S target-site mutation in conferring resistance to glyphosate in a goosegrass (Eleusine indica) population from the Philippines. Weed Sci. 56:637646
Lorraine-Colwill DF, Powles SB, Hawkes TR, Preston C (2001) Inheritance of evolved glyphosate resistance in Lolium rigidum Gaud. Theor Appl Gen. 102:545550
Mao Q, Huff DR (2012) The evolutionary origin of Poa annua L. Crop Sci. 52:19101922
McElroy JS, Flessner ML, Wang Z, Dane F, Walker RH, Wehtje GR (2013) A Trp574 to Leu amino acid substitution in the ALS gene of annual bluegrass (Poa annua) is associated with resistance to ALS-inhibiting herbicides. Weed Sci. 61:2125
McElroy JS, Walker RH, van Santen E (2002) Patterns of variation in Poa annua populations as revealed by canonical discriminant analysis of life history traits. Crop Sci. 42:513517
Michitte P, De Prado R, Espinoza N, Ruiz-Santaella JP, Gauvrit C (2007) Mechanisms of resistance to glyphosate in a ryegrass (Lolium multiflorum) biotype from Chile. Weed Sci. 55:435440
Norsworthy JK, Smith KL, Scott RC, Gbur EE (2007) Consultant perspectives on weed management needs in Arkansas cotton. Weed Tech. 21:825831
Perez-Jones A, Park KW, Polge N, Colquhoun J, Mallory-Smith CA (2007) Investigating the mechanisms of glyphosate resistance in Lolium multiflorum . Planta. 226:395404
Powles SB, Yu Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol. 61:317347
Pratley J, Baines P, Eberbach P, Incerti M, Broster J (1996) Glyphsoate resistance in annual ryegass. Pages 126p in Virgona J, Michalk D, eds. Proceedings of the 11th annual conference of the Grassland Society of New South Wales. Wagga Wagga, Australia Grassland Society of NSW
Rodrigues JJV, Worsham AD, Corbin FT (1982) Exudation of glyphosate from wheat (Triticum aestivum) plants and its effects on interplanted corn (Zea mays) and soybeans (Glycine max). Weed Sci. 30:316320
Shaner DL, Nadler-Hassar T, Henry WB, Koger CH (2005) A rapid in vivo shikimate accumulation assay with excised leaf discs. Weed Sci. 53:769774
Steinrücken HC, Amrhein N (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase. Biochem Biophys Res Commun. 94:12071212
Wakelin AM, Lorraine-Colwill DF, Preston C (2004) Glyphosate resistance in four different populations of Lolium rigidum is associated with reduced translocation of glyphosate to meristematic zones. Weed Res. 44:453459
Wakelin AM, Preston C (2006) A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res. 46:432440
Yu Q, Cairns A, Powles S (2007) Glyphosate, paraquat and ACCase multiple herbicide resistance evolved in a Lolium rigidum biotype. Planta. 225:499513
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Weed Science
  • ISSN: 0043-1745
  • EISSN: 1550-2759
  • URL: /core/journals/weed-science
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