Our objective was to determine if the chlorsulfuron-induced reduction in assimilate export from leaves can be attributed to a shortage of carbohydrates. Treated canola leaves showed no reduction in carbon fixation or carbohydrate production during the first 24 h, but they exuded only 17 to 27% of the amount of sucrose exuded by corresponding control leaves. Exposure of the leaves to higher concentrations of CO2 (500 vs. 350 μl L−1) resulted in greater net carbon exchange and higher starch content, but failed to overcome the reduction in sucrose export, presumably because of increased carbon allocation to starch.

To determine if cell damage could account for chlorsulfuron inhibition of assimilate transport, the ultrastructure of companion cells and surrounding mesophyll cells was examined following chlorsulfuron treatment. Six hours after treatment of canola leaves, thylakoids in companion cells were swollen and chloroplast structures in mesophyll cells were disorganized. Three days after treatment, the treated leaves contained more starch granules in companion cells than corresponding control leaves, presumably as a result of reduced assimilate export. We conclude that the inhibition of assimilate export in chlorsulfuron-treated canola leaves is associated, at least in part, with the ultrastructural changes described, particularly those in companion cells.

Microsomes (100,000 g pellet containing mixed membrane fractions but primarily endoplasmic reticulum) were isolated from shoots of corn, shattercane, and woolly cupgrass grown from naphthalic anhydride treated or untreated seed to determine if metabolism of bentazon, nicosulfuron, and primisulfuron could be demonstrated in the preparations. Corn is tolerant of all three herbicides, shattercane is tolerant of bentazon, and woolly cupgrass is tolerant of bentazon and primisulfuron. Naphthalic anhydride treatment was required for detectable bentazon, nicosulfuron, and primisulfuron hydroxylation in corn microsomes and for bentazon hydroxylation in woolly cupgrass microsomes. Bentazon hydroxylation was low, but detectable, in microsomes from shattercane shoots without naphthalic anhydride treatment. Naphthalic anhydride-treated corn microsomes hydroxylated 292, 120, and 52 pmol mg−1 protein min−1 of bentazon, nicosulfuron, and primisulfuron, respectively. Primisulfuron (19 pmol mg−1 protein min−1), but not nicosulfuron, was hydroxylated in woolly cupgrass microsomes. Neither nicosulfuron nor primisulfuron was hydroxylated in shattercane microsomes. Bentazon and primisulfuron inhibited nicosulfuron hydroxylation in corn microsomes. Bentazon, but not nicosulfuron, also inhibited primisulfuron hydroxylation in the corn microsomes. This indicates that the three herbicides can interact at the same cytochrome P-450(s) in corn. Primisulfuron hydroxylation was not inhibited by either bentazon or nicosulfuron in woolly cupgrass microsomes. This suggests that the cytochrome P-450(s) for primisulfuron hydroxylation are different between corn and woolly cupgrass. Also, bentazon hydroxylation in corn and shattercane microsomes was inhibited by the cytochrome P-450 inhibitor tetcyclasis, while that in woolly cupgrass was not. Again, this suggests a difference in the cytochrome P-450(s) responsible for bentazon metabolism among the species. Although absolute conclusions comparing in vitro microsomal activities to whole plant herbicide tolerance cannot be made because it is unknown whether the same cytochrome P-450(s) are studied in microsomes from naphthalic anhydride-treated tissue as are responsible for in vivo herbicide metabolism, there was a broad correlation between metabolism of a particular herbicide in microsomes of a species and the species' tolerance of that herbicide.

Greenhouse and field experiments were conducted to study competition between wheat and rigid ryegrass or between wheat and cowcockle using additive series and growth analysis. Wheat was the dominant competitor with either weed. One wheat plant was as competitive as 1 1 or 19 rigid ryegrass plants in greenhouse and field experiments, respectively. One wheat plant was as competitive as three to 24 cowcockle plants, depending on environmental conditions. Dry weight of roots, leaves, stems, and spikes or capsules responded similarly to the effects of competition between wheat and rigid ryegrass or cowcockle. Shoot dry weight was the easiest, fastest, and least expensive component to measure competition. Growth analyses of individual plants showed that wheat had a greater leaf area, shoot and root dry weight, and absolute growth rate than rigid ryegrass or cowcockle, particularly early in the season. A range of 120 to 240 wheat plants m−2 can minimize rigid ryegrass or cowcockle competition and achieve an acceptable grain yield in semiarid areas in Morocco.

To predict weed emergence and help farmers make weed management decisions, we constructed a mathematical model of seed germination for green and redroot pigweed based on temperature and water potential (moisture) and expressing cumulative germination in terms of thermal time (degree days). Empirical observations indicated green pigweed germinated at a lower base temperature than redroot pigweed but the germination rate of redroot pigweed is much faster as mean temperature increases. Moisture limitation delayed seed germination until 23.8 C (green pigweed) or 27.9 (redroot pigweed); thereafter, germination was independent of water potential as mean temperatures approached germination optima. Our germination model, based on a cumulative normal distribution function, accounted for 80 to 95% of the variation in seed germination and accurately predicted that redroot pigweed would have a faster germination rate than green pigweed. However, the model predicted that redroot pigweed would germinate before green pigweed (in thermal time) and was generally less accurate during the early period of seed germination. The model also predicted that moisture limitation would increase, rather than delay, seed germination. These errors were related to the mathematical function chosen and analyses used, but an explicit interaction term for water potential and temperature is also needed to produce an accurate model. We also tested the effect of mean temperature on shoot elongation (emergence) and described the relationship by a linear model. Base temperatures for shoot elongation were higher than for seed germination. Shoot elongation began at 15.6 and 14.4 C for green and redroot pigweed, respectively; they increased linearly with temperature until the optimum of 27.9 C was reached. Elongation was dependent on completion of the rate-limiting step of radicle emergence and was sensitive to temperature but not moisture; hence, elongation was sensitive to a much smaller temperature range. Beyond mathematical changes, we are testing our model in the field and need to link it to ecophysiological, genetic, and spatially explicit population processes for it to be useful in decision support for weed management.

Field studies were conducted at the Main Experiment Station, Fayetteville, AR, in 1994 and 1995 to evaluate the effect of postemergence herbicides applied at late-season timings on sicklepod seed production and viability. AC 263,222, dicamba, glyphosate, glufosinate, and paraquat were applied at five rates (zero; labeled rate; one-half, one-fourth, and one-eighth the labeled rate) to three sicklepod growth stages (bud formation, flowering to 9-cm pods, and 15- to 30-cm pods). The greatest reduction in seed production occurred when herbicides were applied at the flowering to 9-cm pod growth stage. All herbicides applied at one-half the labeled rate to sicklepod at bud formation and at the flowering to 9-cm pod growth stage reduced seed production greater than 80%, except glufosinate. Regardless of herbicide, rate, or application timing, viability of remaining seed was ≥ 90% with all treatments.

Redroot pigweed is a common weed in sorghum fields throughout the southcentral United States including Kansas. In 1994 and 1995, field studies were conducted at two sites near Manhattan, KS, to determine the influence of redroot pigweed densities and times of emergence on sorghum yield and yield components. Redroot pigweed was sown at densities of 0.5, 1, 2, 4, and 12 plants meter−1 of row within a 25-cm band over the sorghum row at planting and at the three- to four-leaf stage of sorghum. A rectangular hyperbola was used to describe the relationship between crop yield loss and weed density. Because of the instability of both coefficients I (percentage yield loss at low weed density) and A (percentage yield loss at high weed density), our results do not support the use of a model based exclusively on weed number to estimate sorghum yield loss across all locations within a region. A quadratic polynomial equation that accounts for the time of weed emergence relative to the crop growth stage is suggested as an alternative method to estimate sorghum yield loss. At the densities studied, the time of pigweed emergence relative to the sorghum leaf stage was critical for the outcome of sorghum-pigweed competition. Significant sorghum yield losses occurred only when pigweed emerged before the 5.5-leaf stage of sorghum. An examination of yield components suggested that the yield loss was a result of a reduction in number of seeds per head.

Proso millet occurs both as a crop and a weed in North America. In 1970, an olive-black seeded biotype called ‘wild proso millet’ was found as an aggressive weed in row crops in Minnesota and Wisconsin and has since spread over a large area. We used Random Amplified Polymorphic DNA (RAPD) to assess genetic relationships among biotypes, measure genetic variation within wild proso millet across its range, and detect hybridization between wild proso millet and crop biotypes of proso millet. We found 97 RAPD genotypes among 398 individuals: 69 wild proso millet genotypes, 26 crop and crop-like weed genotypes, and two hybrid genotypes. Five RAPD markers consistently differentiated wild proso millet from crop cultivars and crop-like weeds. About 10% of the genotypes had at least one marker of the other type, suggesting possible hybridization between wild proso millet and crop biotypes. Most genotypes occurred in only one or two of the over 100 populations tested. The most widespread wild proso millet genotype occurred in 12 populations distributed in North Dakota, Minnesota, Illinois, and Wisconsin. More genetic variation exists among populations of wild proso millet than expected for a plant that presumably experienced a severe genetic bottleneck only 20 generations ago. Hypermutation rates and crossing between wild proso millet and crop cultivars could not account for the degree of genetic variation found in wild proso millet. The pattern of genetic variation among wild proso millet populations suggests multiple introductions of wild proso millet to North America.

Exotic annual weeds on western U.S. rangelands owe at least part of their invasion success to prolific seed production. Reproductive output is determined largely by timing of flowering in relation to soil moisture, light, and competing vegetation. This study investigated the influence of temperature (including vernalization), photoperiod, and intraspecific competition on reproductive phenology in three populations (two varieties, typica and bracbypappa) of common crupina. Monthly plantings at four densities were grown in field plots through two consecutive cycles, and the date each plant reached bud and flowering stage was recorded. Development of greenhouse-grown plants from the same populations was compared with those grown outside for 4, 6, 8, and 10 wk of cold induction. Reproductive development in common crupina was influenced by all factors investigated: temperature, photoperiod, density, and vernalization. Conditions fostering the shortest thermal time from emergence to floral induction were a period of cold temperatures/short photoperiod as seedlings followed by warming temperatures and lengthening photoperiod. Both varieties of common crupina responded quantitatively to vernalization, but lack of vernalization caused a greater delay of flowering in var. brachypappa than in var. typica. Vernalization contributed to convergence of spring flowering among the August to December plantings, but earlier emergence and lower plant density greatly increased final plant size and fecundity. Lack of vernalization, high temperatures, and shortening day length delayed flowering of spring/summer emergents, increasing the probability of succumbing to drought before producing seed.

Thermal time models for predicting phenological development require an estimate of base temperature, an attribute not previously defined for common crupina, a Mediterranean winter annual introduced in western North America. The stage of seed germination was selected for estimating base temperature, because facilities were available for experiments over a range of constant temperatures and base temperature is relatively constant throughout the life cycle in other species. Achenes from three populations of common crupina, including two varieties, typica and brachypappa, were produced under uniform conditions. Cumulative germination was recorded at 12 h intervals for achenes in darkness and optimum moisture at 23 constant temperatures from 4 to 17 C. The time course of germination was best described by a logistic growth curve from which time to 50% germination was estimated. A parabolic model provided the best fit in a regression of germination rate (reciprocal of time to 50%) against the temperature gradient, yielding base and optimum temperatures of 1 and 10.5 C, respectively. Bootstrap confidence intervals indicated no significant difference in base and optimum temperatures in germination between the two varieties nor between two populations of var. typica of common crupina introduced in the northwestern United States.

Plumeless thistle is a European Asteraceae naturalized in Argentina, where it is a troublesome pasture and range weed. A European weevil (Rhinocyllus conicus) was imported for biocontrol of this thistle. Oviposition patterns, egg to adult survival, and the weevil's effect on the production of achenes by thistles were monitored over 2 yr. The oviposition period did not begin simultaneously in all thistle populations, but finished in late November. Egg to larvae survival was 63% and egg to adult survival was approximately 50%. No important egg predators were detected. The weevils destroyed 80% of the propagules per capitulum and showed a slight tendency to adapt to the plumeless thistle cycle.

Field studies were conducted at Morden, Manitoba, in 1994 and 1995 to investigate the effect of crop growth stages on canola and sunflower tolerance to sublethal doses of thifensulfuron:tribenuron (2:1). Thifensulfuron:tribenuron at doses of 0, 0.23, 0.45, 0.9, 1.8, and 3.6 g ai ha−1 plus a nonionic surfactant at 0.5% v/v were applied to canola and sunflower at the two- to three-leaf, four- to five-leaf, and six- to seven-leaf stages. Crop leaf stage at the time of application affected tolerance of both crops to thifensulfuron:tribenuron. Crop injury was lowest, and flowering, seed yield, and seed oil content were least affected when low doses of thifensulfuron:tribenuron were applied at the two- to three-leaf stage. At the highest dose, there was little practical difference among growth stages since yield of both crops was severely reduced. Producers with drift-affected canola or sunflower can expect less effect on crop yield when thifensulfuron:tribenuron injury occurs during early crop growth.

Green kyllinga is a relatively new weed species in well-irrigated turfs in the southwest US. To understand the germination requirements of green kyllinga, the effects of planting depth, temperature, pH, and osmotic potential were determined. Green kyllinga emergence was sensitive to planting depth. At 21 days after planting (DAP), emergence of green kyllinga was 60% when planted at the soil surface but only 20% at 0.5 cm and less than 5% at 1 cm. Germination occurred at temperatures between 17 and 30 C but was notably better between 20 and 24 C. Germination was uniform from pH 5.5 to 9.5. Germination decreased between the water potentials −0.1 to −0.6 MPa, although germination was high at water potentials found in irrigated turf. The environmental conditions found in nonstressed turf areas in Arizona will likely support the establishment of green kyllinga. The efficacy of herbicides for the control of green kyllinga was also determined. Control of kyllinga (95% or greater) was obtained with preemergence applications of oxadiazon, dithiopyr, and metolachlor, and with postemergence applications of DSMA, halosulfuron-methyl, and imazaquin.

Glyphosate applied at rates of 0, 0.3, 0.6, 1.1, 2.2, 3.4, 4.5, 6.7, 9.0, and 13.4 kg ai ha−1 was evaluated for the control of greenhouse-grown Erythroxylum novogranatense var. novogranatense and E. coca var. coca plants. Glyphosate reduced live weight of both species, 6 wk after treatment, at rates as low as 0.6 kg ha−1, but was more effective in reducing live weight and regrowth on E. novogranatense than E. coca. Glyphosate at 1.1 kg ha−1 killed 100% of E. novogranatense plants 5 mo after treatment. Glyphosate rates of 2.2 kg ha−1 and higher reduced regrowth of E. coca plants 65 to 100%, independent of leaf age at time of spraying. Control of regrowth persisted for over 17 mo after treatment, with 37% of E. coca plants dying at rates of 13.4 kg ha−1 between 6 mo and 1 yr after treatment. Glyphosate, applied up to 6.7 kg ha−1, had no significant effect on regrowth of E. coca when plants were defoliated 24 h before treatment. Glyphosate at 2.2 kg ha−1 effectively killed E. novogranatense plants and reduced leaf production of E. coca plants.

Economic decision rules for postemergence herbicide control of barnyardgrass in corn were developed. Damage and control functions that formed the basis of an economic model were estimated. Barnyardgrass density and time of emergence relative to the crop were fundamental to calculate the damage function. The control function described barnyardgrass dry weight response to variable doses of two herbicides. Both the biologist's and economist's weed control decision rules, derived from the economic model, were influenced by time of weed emergence relative to the crop, corn yield, and price. Inclusion of time of weed emergence relative to the crop improved our interpretative ability of derived decision rules. The biologist's threshold weed density was more sensitive to changes in parameter values than the economist's optimal herbicide dose strategy. Herbicide use with recommended label dose was greater than either the economically optimal or the biologically effective doses. Use of the biologically effective dose for postemergence weed control decisions was cost efficient and could be of practical significance to corn growers.

Research was conducted in 1994 and 1995 to evaluate the field mobility of flumetsulam in three soils of varied texture and organic matter content but constant pH (pH = 6.0 ± 0.1). Flumetsulam was monitored to a depth of 122 cm at 28, 56, and 84 days after treatment (DAT). Flumetsulam concentrations were determined by cotton bioassay, with separate standard curves for various soil–depth combinations. Following a preemergence application of flumetsulam at 224 g ai ha−1, the herbicide was primarily limited to the upper 8 cm of soil, regardless of soil type, year, or DAT. Exceptions to this typically occurred following substantial rainfall amounts early in the season. Beyond 28 DAT, no significant concentrations of flumetsulam were detected below 15 cm. Results from this research suggest that leaching is not a significant route of flumetsulam dissipation in the field.

Field research was conducted in 1994 and 1995 to monitor the dissipation of flumetsulam in five Mississippi soils for a period of 16 wk. These soils allowed evaluation of flumetsulam persistence across three soil series with a relatively constant soil pH of 6.0, as well as across three soils of varied pH from 6.0 to 7.6 within a constant soil series. Bioavailability was determined with a rice root length bioassay. Predicted half-lives ranged from 20 to 26 d in 1994 and from 23 to 46 d in 1995. Persistence decreased with increasing cumulative rainfall and decreasing organic matter content (within a range of 1.2 to 3.5%). Soil pH had no measurable effect on flumetsulam persistence.

Laboratory studies were conducted to evaluate flumetsulam adsorption and mobility in seven Mississippi soils of different organic matter content, pH, and texture. Adsorption isotherms were determined for all soils using a 1:1 (soil: water) technique. In six of seven soils, Freundlich n constants were close to unity, suggesting a partitioning-like adsorption mechanism for flumetsulam. Mobility was examined using packed soil columns. 14C-flumetsulam recoveries in leachate ranged from 1 to 70% and were influenced by both organic matter content and soil pH. However, the effects of organic matter content and soil pH were not independent. Consequently, clear relationships between flumetsulam mobility and either organic matter content or soil pH were not established across all soils. However, among soils of similar pH (7.5 ± 0.3), mobility decreased linearly (R2 = 0.75) as organic matter content increased from 0.7 to 3.6%. Across soils with similar organic matter content (3.9 ± 0.3%), mobility increased linearly (R2 = 0.98) as soil pH increased from 5.3 to 7.2. Net adsorption constants (Kd) provided a more accurate assessment of flumetsulam mobility across all soils than Koc.

Activity, adsorption, mobility, and field persistence of one emulsifiable concentrate (EC) and three microencapsulated (ME) formulations of alachlor were studied with petri dish bioassay, based on root response of oats grown in sand or soil. Both bioassays indicated that activity of all formulations was increased with increasing herbicide concentration. EC-alachlor in sand showed the highest activity, while ME-alachlorL and ME-alachlorA, the lowest; the activity of ME-alachlorC was intermediate. In silty clay loam soil, EC-alachlor had the highest activity, while ME-alachlorL had the lowest; and ME-alachlorA showed intermediate activity and was similar to that of ME-alachlorC More alachlor was adsorbed on the soil or remained encapsulated (not biologically available) after ME-alachlorL and ME-alachorA application compared to EC-alachlor. An intermediate amount of alachlor was adsorbed or remained inside the capsules when the ME-alachlorC formulation was applied. Greater amounts of alachlor were leached through a silty clay loam after EC-alachlor application compared to the three ME-alachlor formulations. Biologically available alachlor was not detected below 15 or 10 cm after application of EC- and ME-alachlor formulations, respectively. All alachlor formulations applied alone or in mixture with atrazine showed similar field persistence. Biologically available alachlor was not detected in the 0- to 10-cm soil depth 30 d after their application. All alachlor formulations applied alone gave excellent control of redroot pigweed and black nightshade, but only partial control of jimsonweed. None of the herbicide treatments showed any detrimental effect on corn, and all of them increased corn yield to the level of weed-free control.