Introduction
Recent interest in zeaxanthin began with the discovery that it is a major carotenoid in the retinal pigment of the eyeReference Bone, Landrum and Tarsis1. The macula region of the eye has a particularly high concentration of pigment that is almost 1 mm within the macula and is visibly discernable as a yellow spot in the central retinaReference Landrum and Bone2. That is, the concentration of the macular pigment is three orders of magnitude above that in normal serum. Several workers have now shown that macular pigment is composed principally of three isometric carotenoids, lutein, zeaxanthin and meso-zeaxanthin (MZ), present in the approximate proportions 2:1:1 in the retinaReference Bone, Landrum and Tarsis1–Reference Bone, Landrum, Hime, Cains and Zamor4. Landrum & BoneReference Landrum and Bone2 found these pigments represented 72% of the total carotenoid content in the eye and other carotenoids are only present in very much smaller amounts. For example, Handelman and colleagues detected β-cryptoxanthin present but β-carotene, which is predominant in serum, was less than 1%Reference Handelman, Snodderly, Adler, Russett and Dratz5. These workers also noted that there were differences in the ratios of lutein and zeaxanthin in the central and peripheral retina, with zeaxanthin two times more concentrated than lutein in the fovea but lutein more dominant in the parafoveal regionReference Handelman, Snodderly, Adler, Russett and Dratz5, Reference O'Connell, Neelam, Nolan, Au Eong and Beatty6.
The stereochemical isomerism for both lutein and zeaxanthin poses interesting questions concerning potential functions of the different forms that are present. In fact macular lutein is composed solely of the most abundant natural stereoisomer ((3R,3′R,6′R)-β-ɛ-carotene-3,3′-diol) but zeaxanthin has been demonstrated to be primarily composed of two stereoisomers in the retina, the 3R,3′R form and the 3R,3′S (MZ) form (Fig. 1)Reference Landrum and Bone2, Reference Bone, Landrum, Hime, Cains and Zamor4. Evidence suggests that 3R,3′R-zeaxanthin is the major natural form and this is the only form found to exist in higher plants and most shellfish but all three optical isomers of zeaxanthin are found in the shrimp carapace, depot fat tissues in turtles and in the integument of twenty species of fishReference Maoka, Arai, Sinuzu and Matsuno7. No MZ was detected in hens' egg yolk. As the MZ-containing foods are minor components of human diets, it is interesting to speculate why MZ should be a major form in macular pigment and what is the possible source of MZ in retinal tissue. Bone et al. Reference Bone, Landrum, Hime, Cains and Zamor4 and othersReference Khachik, de Moura, Zhao, Aebischer and Bernstein8, Reference Bone, Landrum, Friedes, Gomez and Kilburn9 suggested that dietary lutein may be the source of MZ in the eye and this has recently been confirmed in monkeysReference Johnson, Neuringer, Russell, Schalch and Snodderly10. In the present paper it is proposed to (1) review the foods known to provide lutein and zeaxanthin, (2) relate the amounts of the xanthophyll pigments present in human diets with the concentrations found in human plasma, (3) examine the influence of different food and xanthophyll supplements on plasma concentrations and (4) examine plasma concentrations of xanthophyll carotenoids in studies of patients with macular disease and matched controls or where attempts have been made to relate plasma concentrations to macular pigment density.
The main xanthophyll carotenoids found in the macula. (A) Zeaxanthin (3R,3′R-dihydroxy-β,β-carotene); (B) meso-zeaxanthin (3R,3′S-dihydroxy-β,β-carotene); (C) lutein (3R,3′R,6′R-dihydroxy-β,ɛ-carotene).
There is as yet no direct evidence that either lutein or zeaxanthin will protect against macular disease either by prevention or treatmentReference Trumbo and Ellwood11. However, the direct relationship between dosing with the different xanthophyll carotenoids and increases in macular pigment densityReference Landrum, Bone, Joa, Kilburn, Moore and Sprague12–Reference Hammond, Johnson, Russell, Krinsky, Yeum, Edwards and Snodderly14 may be evidence that dietary intake and plasma concentrations of xanthophyll carotenoids are inversely related to the risk of macular disease. Very recently a specific binding protein (GSTP1) for zeaxanthin has been isolated from human retinasReference Bhosale, Larson, Frederick, Southwick, Thulin and Bernstein15. Dietary (3R,3′R)-zeaxanthin displayed high affinity for the protein with an apparent dissociation constant (K d) of 0·33 μm as also did (3R,3′S-meso)-zeaxanthin with only slightly less affinity (K d 0·52 μm) but (3R,3′R,6′R)-lutein displayed no affinity. The binding protein is a glutathione-S-transferase (GST). GST has been shown to exist in twelve different classes and within each class many sub-isoforms can exist. In man only one subclass of the Pi isoform is known to be expressed in human tissue, GSTP1. GSTP1 is widely expressed in human epithelial tissue and known to be in the retina but its cellular localisation was unknown until this reportReference Bhosale, Larson, Frederick, Southwick, Thulin and Bernstein15. Using immunocytochemical techniques, the authors showed that high levels of GSTP1 are specifically localised in the parafoveal plexiform layers of the human macula and correlate well with the xanthophyll pigment distribution reported by Snodderly et al. Reference Snodderly, Auran and Delori16. The localisation of GSTP1 suggests that its primary role is to take up zeaxanthin from the blood. However, GSTP1 may possibly have other activities particularly since one of the isomers, (3R,3′S-meso)-zeaxanthin, is not normally present in blood. Another GST isoform, GSTA3-3, can catalyse a double-bond shift reaction in steroid biochemistryReference Johansson and Mannervik17. GSTP1 may be involved in analogous activity to convert (3R,3′R,6′R)-lutein to (3R,3′S-meso)-zeaxanthin as now shown to take place in monkeysReference Johnson, Neuringer, Russell, Schalch and Snodderly10.
The identification and isolation of a specific binding protein for the two zeaxanthin diastereoisomers opens the possibility that activity-altering polymorphisms on the gene for GSTP1 may alter its binding properties, enzyme activity, interaction with intracellular mediators, etc, all of which may influence the risk of macular diseaseReference Bhosale, Larson, Frederick, Southwick, Thulin and Bernstein15. In such a situation, the availability of the non-dietary form of zeaxanthin, namely (3R,3′S-meso)-zeaxanthin, may be a timely addition to armoury of supplements to reduce the risk of macular disease.
The aetiology of macular disease is still only poorly understood and both genetic and environmental factors may be involved. One environmental factor that may be especially important is exposure to sunlight and history of exposure to blue light has been associated with an increased risk of macular diseaseReference Taylor, West, Munoz, Rosenthal, Bressler and Bressler18. The retina is highly active metabolically and has a higher blood flow than other tissuesReference Sickel19. High metabolic activity and the simultaneous presence of light and O2 will generate reactive oxygen species which in the absence of suitable antioxidants can damage PUFA that are rich in the photoreceptor outer segmentsReference Schalch, Emerit and Chance20. The macula in particular may be intermittently exposed to high light intensity and the high concentration of carotenoids may exert a protective role against oxidative damageReference Handelman, Dratz, Reay and Van Kuijk3. Carotenoids are potent quenchers of singlet oxygenReference Foote and Denny21 and lipid radicalsReference Burton and Ingold22, Reference Chopra, Willson, Thurnham, Canfield, Olsonand and Krinsky23. Lutein and zeaxanthin both absorb blue light with a maximum wavelength of about 450 nm and their location in Henle's fibre layer, just in front of the photoreceptors, is appropriate to this filter action. The importance of carotenoids in protecting the eye has been shown in macaque monkeys raised on a carotenoid-free diet for 5 yearsReference Malinow, Feeney-Burns, Peterson, Klein and Neuringer24, where there was a loss of macular pigment and more drusen and other indicators of photic damage were found. One of the first intervention studies which found evidence that zeaxanthin might protect the retina against light was reported by Dorey et al. Reference Dorey, Toyoda, Thompson, Garnett, Sapunzatkis, Craft, Nichols and Cheng25. Quails were fed a carotenoid-free diet or with zeaxanthin (5 mg/kg) for 3 months. Birds were then exposed to intermittent white light for 28 h to induce photic damage to the retina. After 14 h in the dark, eyes were excised to determine zeaxanthin in the retina and to measure the number of apoptotic cells. The number of apoptotic rod and cone photoreceptor cells was significantly lower in the treated compared with the control birds. Furthermore those retinas containing more zeaxanthin, as assessed by HPLC, seemed better protected than those with less. Other xanthophyll-intervention studies to increase macular pigmentation are discussed later in the present review.
Dietary intake and bioavailability of xanthophylls
The predominant xanthophyll pigment in the diet is lutein and relatively high concentrations occur in dark green vegetables together with the carotenes, especially β-carotene. The intake of lutein (and zeaxanthin) in the USA is between 1 and 3 mg/d; white individuals tend to be nearer the bottom and blacks nearer the top of the intake spectrumReference Ciulla, Curran-Celentano, Cooper, Hammond, Danis, Pratt, Ricardi and Filloon26–Reference Mares-Perlman, Fisher, Klein, Palta, Block, Millen and Wright28. Analysis of survey data from US National Health Interviews between 1987 and 1992 suggests there was a significant decline in the consumption of dark green leafy vegetables that reduced lutein (and by proxy zeaxanthin) intakes particularly in white womenReference Nebeling, Forman, Graubard and Snyder29. The content of zeaxanthin in the diet is usually much lower than either β-carotene or lutein and one recent estimate based on National Health and Nutrition Examination Survey (NHANES) data has suggested that the lutein:zeaxanthin ratio in the US diet is 5:1Reference Hartmann, Thurmann, Spitzer, Schalch, Manner and Cohn30. Because of the low amounts of zeaxanthin in the diet, there are far fewer studies that have assessed its bioavailability than there are of lutein. However, as the structure of the two xanthophyll carotenoids is very similar, studies on lutein bioavailability may assist in understanding zeaxanthin bioavailability.
Bioavailability of lutein in vegetables
Several studies have examined the plasma responses in lutein and sometimes zeaxanthin concentrations to dietary and supplemental lutein (Table 1). The first experiment shown in the tableReference van het Hof, Brouwer, West, Haddeman, Steegers-Theunissen, van Dusseldorp, Weststrate, Eskes and Hautvast31 illustrates plasma responses to lutein in a low- and high-vegetable diet, and to a third group receiving a low-vegetable diet and a lutein supplement. The authors reported that dietary lutein was absorbed 67% as efficiently as the supplemental lutein. Zeaxanthin was not assessed as intakes were not known but it should also be noted that plasma zeaxanthin concentrations increased more in the high vegetable group than the supplement group. The lutein supplement is reported as containing 1·4% zeaxanthin, i.e. approximately 126 μg/d. In contrast, if the amount of zeaxanthin in vegetables is about 20% of the lutein, then there could be about 2 mg zeaxanthin/d in the high-vegetable diet.
Changes in plasma carotenoid concentrations in subjects in industrialised countries following consumption of vegetable foods with or without pure lutein
TP, tomato puree.
* P < 0·05.
† Zeaxanthin in meals eaten was not quantified, so change in concentration of zeaxanthin recorded as a percentage of baseline plasma concentration.
‡ For experimental details and sources of zeaxanthin, see Table 3 for more information.
§ Vegex lutein esters mainly palmitate (0S30; Quest International Ireland Ltd, Dublin, Republic of Ireland).
‖ Low-vegetable diet described as being comparable with the average vegetable intake of the Dutch population.
¶ Crystalline lutein from marigold flowers containing 5% zeaxanthin (FloraGLO; Kemin Industries, Inc., Des Moines, IA, USA).
†† All-trans-lutein purified from marigold flowers contained 1·4% all-trans-zeaxanthin (Kemin Industries, Inc.).
In the second experiment shown in Table 1Reference Castenmiller, West, Linssen, van het Hof and Voragen32, the authors tested the effects of the different processing methods on the bioavailability of lutein in spinach. However, none of the processing methods influenced relative bioavailability that ranged between 45 and 54% of the supplemental lutein in the different groups. Thus bioavailability of lutein in spinach (50%) and in other green vegetables (67%) was of a similar magnitude in the two studies. However, the plasma response to the pure lutein supplement in the Castenmiller et al. study was two times higher than that reported by van het Hof et al. Reference van het Hof, Brouwer, West, Haddeman, Steegers-Theunissen, van Dusseldorp, Weststrate, Eskes and Hautvast31 (0·116 compared with 0·053 μmol/l per mg lutein, respectively), even though the supplemental lutein was from the same supplier and in both studies it was incorporated in the same carrier (a salad dressing). Thus it would appear that lutein absorption from a supplement taken with a vegetable-containing meal is impaired by the presence of the vegetables.
The study of Roodenburg et al. Reference Roodenburg, Leenen, van het Hof, Weststrate and Tijburg33 showed the importance of dietary fat for lutein absorption. Although the authors found that 3 g fat was satisfactory for α- and β-carotene absorption (data not shown), there was a three-fold difference in lutein absorption between diets containing 3 and 36 g/meal. However, differences in fat intake are unlikely to explain any of the differences in the results in the other studies in Table 1 as all had a minimum of 30 g fat/meal but the plasma response to supplemental lutein in this experiment, even in the high-fat group, was relatively poor (0·048 μmol/l per mg lutein) and comparable with the study by van het Hof et al. Reference van het Hof, Brouwer, West, Haddeman, Steegers-Theunissen, van Dusseldorp, Weststrate, Eskes and Hautvast31. Also comparable with that study, volunteers received supplemental lutein together with 160 g vegetables. The vegetables contained almost no carotenoids (lutein < 0·02 mg/meal) but appeared to impair the plasma lutein response to the supplement.
The last study in Table 1 looked at possible interference in lutein absorption by lycopene in tomato purée or as the pure compound. Although chylomicron carotenoid responsesReference Tyssandier, Cardinault, Caris-Veyrat, Amiot, Grolier, Bouteloup, Azais-Braesco and Borel34 did suggest that supplements of lutein or lycopene decreased chylomicron lycopene or lutein respectively there was no evidence of interactions in the plasma carotenoid responses 3 weeks after dietary supplementation (Table 1). The relative plasma lutein responses to spinach (about 12 mg lutein/meal) alone or with tomato paste of supplemental lycopene were 0·075, 0·062 and 0·063 μmol/l per mg respectively. In contrast the lutein response to supplemental lutein given with tomato paste (0·085 μmol/l per mg) was 37% higher than that when the lutein was given as spinach with tomato paste (0·062 μmol/l per mg). The higher response of supplemental lutein demonstrates the higher relative bioavailability of supplemental as opposed to food lutein but it was still not as high as when lutein was given without any vegetable food in the meal (0·116 μmol/l per mg)Reference Castenmiller, West, Linssen, van het Hof and Voragen32. These data therefore suggest that the presence of vegetables with or without carotenoids and including tomato paste depresses the plasma response to supplemental lutein.
In conclusion, although van het Hof et al. Reference van het Hof, Brouwer, West, Haddeman, Steegers-Theunissen, van Dusseldorp, Weststrate, Eskes and Hautvast31 reported that the relative bioavailability of lutein from mixed vegetables was only 67% that of supplemental lutein, the response they obtained for supplemental lutein with vegetables (0·053 μmol/l per mg) was low when compared with that obtained by Castenmiller et al. Reference Castenmiller, West, Linssen, van het Hof and Voragen32 (0·116 μmol/l per mg) who provided lutein as a supplement without food carotenoids. None of the responses in the other two Dutch papersReference van het Hof, Brouwer, West, Haddeman, Steegers-Theunissen, van Dusseldorp, Weststrate, Eskes and Hautvast31, Reference Roodenburg, Leenen, van het Hof, Weststrate and Tijburg33 achieved even half the response of Castenmiller et al. Reference Castenmiller, West, Linssen, van het Hof and Voragen32 and even though those from the French study were somewhat higher, they too confirm that the response to supplemental lutein is depressed by food carotenoids.
Bioavailability of lutein and zeaxanthin from eggs
Six studies have assessed plasma responses to egg lutein and three for zeaxanthin, to give some information on bioavailability (Table 2). In the study by Handelman et al. Reference Handelman, Nightingale, Lichtenstein, Schaefer and Blumberg35 the equivalent of 1·3 eggs was fed daily with two different fats (maize oil and beef tallow as two-thirds of the total dietary fat). Analyses of egg yolks by the authors indicated the amounts of lutein (0·38 mg) and zeaxanthin (0·28 mg) fed daily. After about 32 d the increases in plasma lutein and zeaxanthin in the recipients in the tallow- and maize oil-fed groups respectively were 0·094 and 0·134 (μmol/l lutein) and 0·068 and 0·056 (μmol/l zeaxanthin). There might have been a slightly better lutein response in the maize oil than the tallow group but the mean responses were calculated to assess the increases of the two carotenoids per mg dose. The average plasma responses for lutein (0·3 μmol/l per mg) and zeaxanthin (0·221 μmol/l per mg) were more than double any of the responses by lutein-in-oil groups in the studies described in Table 1. Unfortunately, there was no lutein-in-oil control group with which to compare the egg-lutein response. The latter is desirable because of the large variation in results both between and within laboratories. However, the apparently high bioavailability of the yolk xanthophylls is probably due to the small amount of xanthophyll supplement (0·66 mg/d) thoroughly solublised in the high content of egg phospholipid. By comparison, the amounts of pure lutein given in the vegetable-feeding studies ranged from 6 to 12 mg/d.
Changes of plasma xanthophyll concentrations after the consumption of eggs
* P < 0·05, ** P < 0·01, *** P < 0·001.
† Final minus baseline concentrations expressed as μmol/l and as μmol/l per mg daily intake of respective carotenoids.
‡ Commercial chicken eggs purchased in autumn 1997 in the Boston metropolitan area. Analyses done on eggs classified as large, except one which was extra large.
§ Chickens were fed marigold extract, and xanthophyll esters in egg were > 85% lutein and zeaxanthin was the other main component.
‖ Only described as vitamin powder.
¶ Obtained from Cognis, Nutrition and Health.
†† Provided by Kemin Industries Inc. (Des Moines, IA, USA).
‡‡ Responses calculated from difference between egg and placebo arms of the trial after lutein and zeaxanthin combined and averages of males and females, hyper- and hypocholesterol groups calculated.
The second study shown in Table 2 also lacked a pure lutein control group with which to assess relative bioavailabilityReference Surai, Macpherson, Speake and Sparks36. The authors recruited two groups of subjects to which they fed one control or one lutein-enriched egg per d for 8 weeks. The mean lutein content of the control eggs was about half that of the eggs used by Handelman and colleagues and the amount of xanthophyll given per d was about one-third, and there was no change in plasma lutein concentration. However, in the group that received the lutein-enriched eggs, there was an increase in plasma lutein of 0·110 μmol/l per mg egg lutein. Thus the response was similar to that produced by pure lutein in oilReference Castenmiller, West, Linssen, van het Hof and Voragen32 (see Table 1) but much lower than that reported by Handelman et al. Reference Handelman, Nightingale, Lichtenstein, Schaefer and Blumberg35. Of the possible reasons for the lower response, fat intake was not mentioned by Surai et al. Reference Surai, Macpherson, Speake and Sparks36 and in an 8-week study, there may have been changes in the intake or type of vegetables consumed during that period. Neither fat intake nor the background dietary intake was controlled.
The third study in Table 2 included four intervention groupsReference Chung, Rasmussen and Johnson37. The supplements (powdered lutein, lutein esters, chopped spinach and lutein-enriched egg providing about 6 mg lutein/d) were incorporated into a frittata using egg white in those groups where egg was not the supplement. Supplements were fed for 10 d and there was 19–20 g oil with each meal. The results showed that egg lutein was three times more bioavailable than the pure lutein preparations; however, the lutein response in all groups was poor (compare with Table 1). Both pure lutein groups in the study of Chung et al. Reference Chung, Rasmussen and Johnson37 showed similar responses (0·022 and 0·019 μmol/l per mg) and although both spinach and egg lutein appeared more bioavailable than the pure lutein preparations (0·032 and 0·067 μmol/l per mg respectively) there were no significant differences in the responses by the different groups. The reason for the poorer overall responses may be a combination of the short intervention and the lower dietary fat than used in most studies. It has been shown that 17–18 d is necessary for both lutein and zeaxanthin to reach more than 90% of plateau plasma concentrationsReference Hartmann, Thurmann, Spitzer, Schalch, Manner and Cohn30, Reference Thurmann, Schalch, Aebischer, Tenter and Cohn38. In this studyReference Chung, Rasmussen and Johnson37 egg lutein appeared more bioavailable than both spinach lutein and pure lutein but very little information was supplied on the pure lutein used.
Three more recent studies are also shown in Table 2Reference Clark, Herron, Waters and Fernandez39–Reference Goodrow, Wilson, Houde, Vishwanathan, Scollin, Handelman and Nicolosi41. In all three studies there are plasma responses to egg lutein >0·3 μmol/l per mg, and only in one study where one of the lutein treatments provided 826 μg/d for 12 weeks was a lower response of 0·136 μmol/l per mg obtainedReference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40. It could be argued that it might take longer for the plasma concentration to reach its plateau in response to large doses but Clark et al. Reference Clark, Herron, Waters and Fernandez39 gave 600 μg egg lutein per d for only 3 weeks and the total response (lutein and zeaxanthin combined) was 0·367 μmol/l per mg. So the reason for the relatively poor response to the high-xanthophyll-containing eggs used by Wenzel et al. Reference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40 is not immediately apparent.
The last two studies in Table 2 also provided information on the plasma zeaxanthin responses to egg zeaxanthin intake. Two of the treatments were very similar: 94 μg/dReference Goodrow, Wilson, Houde, Vishwanathan, Scollin, Handelman and Nicolosi41and 114 μg zeaxanthin/dReference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40 but gave responses of 0·160 and 0·421 μmol/mg respectively while the higher amount given by the latter workers (312 μg/d) gave a response of 0·151 μmol/l per mg. There was some evidence from the lutein responses in the latter studyReference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40 that higher doses may take longer to reach a steady state in the plasma but even the mean plasma concentration in response to the high dose (312 μg, 0·104 μmol/l) was lower than that achieved with the low dose (114 μg egg zeaxanthin/d, 0·149 μmol/l).
In conclusion, four out of the six studies indicated that that egg lutein is three to four times more bioavailable than pure luteinReference Handelman, Nightingale, Lichtenstein, Schaefer and Blumberg35, Reference Clark, Herron, Waters and Fernandez39–Reference Goodrow, Wilson, Houde, Vishwanathan, Scollin, Handelman and Nicolosi41. There were slightly poorer responses in those studies where relatively high intakes of lutein were usedReference Surai, Macpherson, Speake and Sparks36, Reference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40 or where the period of supplementation was shorter than the 3 weeks probably needed to reach equilibriumReference Chung, Rasmussen and Johnson37. In the case of egg zeaxanthin, all responses were >0·150 μmol/l per mg with one particularly high response of 0·42 μmol/l per mg. Some of the variability in the plasma response to egg lutein and zeaxanthin may be due to the very large variability in the content of the xanthophyll pigments in eggs where a 10-fold range was reported for lutein and a 4–5-fold range in the case of zeaxanthinReference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40. Unfortunately, apart from the one study which was only 10 d, none of the egg studies included a pure lutein or zeaxanthin control but the frequency of high plasma responses to egg xanthophyll supplements is a clear indication of the high bioavailability of xanthophyll pigments in egg yolk.
Bioavailability of pure xanthophyll supplements
Pharmacokinetics of lutein and zeaxanthin
Two recent studies by Hartmann et al. Reference Hartmann, Thurmann, Spitzer, Schalch, Manner and Cohn30 and Thurmann et al. Reference Thurmann, Schalch, Aebischer, Tenter and Cohn38 suggest that the pharmacokinetics of lutein and zeaxanthin are similar (Table 3; Fig. 2). In these two studiesReference Hartmann, Thurmann, Spitzer, Schalch, Manner and Cohn30, Reference Thurmann, Schalch, Aebischer, Tenter and Cohn38, supplements of zeaxanthin (1 and 10 mg) and lutein (4 and 20·5 mg) were taken daily in a hard-gelatin capsule by small groups of men and women for 42 d. More than 90% of steady-state concentrations were achieved around day 17–18 for both xanthophylls while the half-life for disposal of xanthophylls after the supplementation appears to be rapid initially (within the period of observation) but slowed down considerably when concentrations approached baseline. Hence disposal half-lives of lutein and zeaxanthin were estimated at 5–7 d and 10 d respectively. The small difference between these measurements are probably related to the periods during which the falling concentrations of the respective xanthophylls were measured, namely 38 d (lutein) and 48 d (zeaxanthin)Reference Thurmann, Schalch, Aebischer, Tenter and Cohn38. Others using depletion studies to estimate terminal half-lives of lutein have reported 33–61 dReference Rock, Swenseid, Jacob and McKee42 and 76 dReference Burri, Neidlinger and Clifford43. What is especially interesting, however, in these studies was that the plasma responses at plateau of zeaxanthin and lutein lay on very similar concentration response lines (Fig. 2), possibly suggesting that the formulations used by these workers was of a similar bioavailability.
Long-term supplementation studies to measure plasma responses and kinetics of lutein, zeaxanthin and meso-zeaxanthin uptake and elimination
* Final concentration shown, as baseline values not given.
† Serum concentration change/mg calculated by assuming baseline lutein to be 0·25 μmol/l.
‡ Source of xanthophyll was Roche Chemicals Ltd.
§ Non-esterified mixture of meso-zeaxanthin, lutein and zeaxanthin obtained from Industrial Organica SA, Monterrey, Mexico.
Steady state plasma concentrations of zeaxanthin (▲) and lutein (○). Amounts shown were fed for 42 d. Regression lines for the separate carotenoids indicate plasma responses of 0·10 and 0·075 μmol per mg daily for zeaxanthinReference Hartmann, Thurmann, Spitzer, Schalch, Manner and Cohn30 and luteinReference Thurmann, Schalch, Aebischer, Tenter and Cohn38 intakes respectively.
Bioavailability of zeaxanthin
Other studies on the bioavailability of zeaxanthin are also shown in Table 3. Khachik et al. Reference Khachik, Beecher and Smith44 reported separate supplementation studies with lutein and zeaxanthin isolated from marigold flowers and Chinese berries (Fructus lycii) respectively. The extracted carotenoids were prepared as suspensions in olive oil (10 mg per 4 ml) and were given in separate experiments to the same three subjects for 21 d. In the case of lutein, plasma concentrations rapidly increased from about 0·200 to between 0·8 and 1·0 μmol/l (5–6-fold) and approached plateau concentrations around day 21. The lutein results are comparable with those shown in Fig. 2. In the case of zeaxanthin, even though the same dose was given, the increase was far less than for lutein. Starting plasma concentrations of zeaxanthin were about 0·03 μmol/l and only increased to about 0·12 μmol/l (far less than the response in Fig. 2). These data suggest that the efficiency of zeaxanthin absorption from an oily suspension is only about 10% that of lutein.
More recently, two studies have been done in which the plasma response has been obtained to a combined supplement of non-esterified lutein, zeaxanthin and MZ suspended in soyabean oil in gelatin capsules. The results for lutein and zeaxanthin are discussed first and those for MZ later. In both cases the source of the material was the same (Industrial Orgánica SA, Monterrey, Mexico) but the composition differed. In the first study, two male subjects took the supplement which provided a mixture of lutein, zeaxanthin and MZ (5·5:1·4:14·9 mg/d or 25:6:68% respectively) daily with a meal for 6 weeksReference Bone, Landrum, Cao, Howard and Thurnham45. In the case of both plasma lutein and zeaxanthin concentrations, there were very large differences in the response to the two xanthophylls by the two subjects. In one subject the plasma responses were similar to those seen in Fig. 2 while in the other subject, responses to both carotenoids were very poor. In the second study there were nineteen volunteers who took capsules containing 20 mg lutein, zeaxanthin and MZ in the proportions 54:6:40 for 21 dReference Thurnham, Tremel and Howard46. The average plasma responses for lutein (0·882 μmol/l) and zeaxanthin (0·16 μmol/l) were reasonably close to those shown in Fig. 2.
In the last study shown in Table 3, most of the subjects (n 100) exhibited features of age-related macular degeneration (AMD). In addition many had hypertension (n 63), diabetes (n 11) or coronary vascular disease (n 20); there were thirty subjects currently taking lipid-lowering drugs, five smokers and three had recently had a stroke. In spite of all these indications of chronic disease, the response to 12 mg lutein/d (0·795 μmol/l) almost exactly corresponds with that shown in Fig. 2. In contrast there was almost no response to the 1 mg zeaxanthin/d (0·010 μmol/l). Thus there is similarity in the plasma zeaxanthin responses from the Roche Chemicals and Industrial Organica preparations except in one of the two subjects reported by Bone et al. Reference Bone, Landrum, Cao, Howard and Thurnham45; however, from the material prepared from gujiReference Khachik, Beecher and Smith44, flavobacteriaReference Landrum, Bone, Joa, Kilburn, Moore and Sprague12, Reference Bone, Landrum, Guerra and Ruiz13 and by Bausch & LombReference Trieschmann, Beatty, Nolan, Hense, Heimes, Austermann, Fobker and Pauleikhoff47 there were very low plasma responses (Fig. 3).
Zeaxanthin steady-state plasma responses from different preparations. All preparations were fed for a minimum of 21 d. For experimental details, sources of zeaxanthin and source of the data shown in the figure, see Table 3. Flav Bac, flavobacteria; Roche, Roche Chemicals Ltd; Ind Org, Industria Organica SA.
All the studies shown in Table 3 fed the xanthophyll supplements for a minimum of 21 d, so the time of supplementation should have been adequate to achieve steady-state concentrations of both luteinReference Thurmann, Schalch, Aebischer, Tenter and Cohn38 and zeaxanthinReference Hartmann, Thurmann, Spitzer, Schalch, Manner and Cohn30. Two studies fed the pigments for the minimum time of 21 dReference Khachik, Beecher and Smith44, Reference Thurnham, Tremel and Howard46, while all the other studies were much longer. There were other differences in the sources of manufacture and in the oils used to suspend the xanthophylls that might influence the bioavailability of zeaxanthin, and the amount of fat taken with the xanthophylls was not always specified. Results suggest the plasma response to lutein is strongly related to the intake of dietary fatReference Roodenburg, Leenen, van het Hof, Weststrate and Tijburg33 and it is possible that different fats may also influence bioavailabilityReference Handelman, Nightingale, Lichtenstein, Schaefer and Blumberg35. The number of subjects in two of the studies on zeaxanthin was very small. Furthermore, the dietary habits or history of these subjects was not described. There is evidence suggesting that a high intake of vitamin A-containing foods strongly depresses the absorption of stable-isotope-labelled β-caroteneReference Hickenbottom, Follett, Lin, Dueker, Burri, Neidlinger and Clifford48, and in dietary intervention studies to increase macular pigment optical density (MPOD), the non-responders to a lutein supplement were those whose previous intake of lutein and zeaxanthin was four times higher than that of those who later respondedReference Hammond, Johnson, Russell, Krinsky, Yeum, Edwards and Snodderly14. Thus previous carotenoid intake can markedly affect response to a carotenoid supplement, and the variable responses to pure lutein described in Table 1 indicate that there is much that is still not known about the absorption of lutein.
Bioavailability of the stereoisomers of zeaxanthin
There is almost no work reported in the literature on the comparative bioavailability of the three stereoisomers of zeaxanthin. In chickens, 3R,3′R-zeaxanthin is slightly better absorbed than is the 3S,3′S-diastereoisomer, but the absorption of the meso (3R,3′S) form is only 40% of that of the chiral forms. However, when a racemic mixture of all three isomers was fed, MZ was better absorbedReference Schiedt, Leuenberger, Vecchi and Glintz49. Bone et al. Reference Bone, Landrum, Cao, Howard and Thurnham45 reported the serum concentrations of natural (3R,3′R) zeaxanthin and MZ in two human subjects who were given a mixed suspension of lutein, zeaxanthin and MZ in soyabean oil in gelatin capsules (5·5:1·4:14·9 mg/d respectively; Industrial Orgánica SA, Monterrey, Mexico) that was taken daily with a meal for 6 weeks. The serum concentrations of zeaxanthin and MZ in one subject increased over this period by 0·043 and 0·044 μmol/l respectively, and in the second by 0·133 and 0·145 μmol/l, i.e. the increase in the serum concentrations were approximately equimolar in both subjects for the two isomers of zeaxanthin. As the relative amount of the 3R,3′S (meso) was ten times greater than that of the 3R,3′R form of zeaxanthin in the supplement, this suggests that the plasma uptake of MZ in man is only 10% of the natural form of zeaxanthin in the diet.
In the second of the two studies using the mixed supplement, the uptake of MZ into the plasma was slightly greaterReference Thurnham, Tremel and Howard46. The change in the plasma MZ concentration after 21 d was 0·026 μmol/l per mg whereas uptake in the above study was 0·003 and 0·01 μmol/l per mg for the two volunteersReference Bone, Landrum, Cao, Howard and Thurnham45. These results for MZ should be interpreted cautiously. The apparently lower response to MZ than zeaxanthin may be because MZ is not a normal dietary component and is not normally found in serum. The serum carotenoids are also found throughout the body in fat deposits, thus any new carotenoid entering the body is likely to be distributed throughout the body before noticeable increases in plasma concentrations occur. It is also possible that MZ is selectively taken up by the optical tissues for incorporation into the macula although it is unlikely that this would have a major impact on plasma concentrations of MZ. The average amount of MZ per human donor eye taken within 24 h of death was reported to be 7·7 ngReference Khachik, de Moura, Zhao, Aebischer and Bernstein8 while even in the supplemented subject with the lower plasma concentration of MZ (0·044 μmol/l45), the amount of MZ in the plasma was about 120 × 103 ng assuming 5 litres to be the blood volume. Thus the relatively lower concentrations of plasma MZ than zeaxanthin following receipt of the mixed supplement may be due to poor bioavailability of MZ and/or a greater uptake of MZ than zeaxanthin into fatty tissues in the body. A resolution to these uncertainties will only be obtained when some stable-isotope-labelled MZ is available and its fate determined following supplementation.
Zeaxanthin in foods
As previously indicatedReference Maoka, Arai, Sinuzu and Matsuno7 very few foods contain MZ. Fish skin is the most likely dietary source but there is no information in food tablesReference Maoka, Arai, Sinuzu and Matsuno7. Zeaxanthin is also a minor dietary component and the amount in the diet will depend very much on the specific foods consumed. United States Department of Agriculture (USDA) Nutrition Coordinating Center food composition tables50 provide zeaxanthin concentrations in twenty-two commonly-eaten foods and indicate that yellow maize (canned) is by far the richest vegetable source of zeaxanthin in the diet (528 μg/100 g); the concentration even exceeding lutein (356 μg/100 g). However, the composition of maize can be variable. The lutein and zeaxanthin contents of the maize used by Hammond et al. Reference Hammond, Johnson, Russell, Krinsky, Yeum, Edwards and Snodderly14 were 270 and 200 μg/100 g respectively. Thus the zeaxanthin content was only half that reported by the USDA. Vegetables and fruits in the USDA report contain on average only one-quarter of the zeaxanthin in maize (125 and 129 μg/100 g respectively) but differed considerably in their content of lutein (3904 and 143 μg/100 g respectively). Thus the lutein:zeaxanthin ratio in vegetables (36:1) and fruits (3·5:1) differs considerably and fruit consumption rather than vegetables is likely to have a larger impact on zeaxanthin consumption in humans. It is reported that the lutein:zeaxanthin ratio in the diet in the USA is 5:1 based on the USDA dataReference Hartmann, Thurmann, Spitzer, Schalch, Manner and Cohn30 while in Europe reports indicate 5·5:1 in fresh fruit and vegetables consumed in a large Spanish surveyReference Granado, Olmedilla, Blanco and Rojas-Hidalgo51, 15:1 in the diet of young, type 1 diabetics and 8:1 in that of age- and sex-matched controlsReference Granado, Olmedilla and Blanco52.
Populations consuming yellow maize as a staple food are likely to have the highest consumption of zeaxanthin. Plasma concentrations of lutein and zeaxanthin in Northern Chinese adults where the staple cereal was yellow maize were 1·0 (sd 0·4) and 0·13 (sd 0·06) μmol/l respectivelyReference Chang-Claude53. Surprisingly, although the plasma lutein values in the Chinese were considerably higher than those found in European or American samples54, the zeaxanthin concentrations were not very different from mean values reported in Europe or America (0·07–0·09 μmol/l)Reference Curran-Celentano, Hammond, Ciulla, Cooper, Pratt and Danis27, Reference Granado, Olmedilla and Blanco52 but this may be attributable to the method of analysis usedReference Bone, Landrum and Tarsis1 and not to green vegetables diluting out the zeaxanthin. (It should be noted that the Chinese samples were measured using a liquid chromatography method that did not separate lutein and zeaxanthinReference Thurnham, Smith and Flora55. Using this method zeaxanthin is normally concealed within the lutein peak; however, in the Chinese samples zeaxanthin was much more obvious and appeared as a smaller peak or shoulder on the side of the lutein peak. It was integrated by the peak-skimming method and that may have underestimated the size of the zeaxanthin contribution.) The blood was taken in the spring when the main vegetable being consumed was Chinese cabbage and the main source of lutein was green leaves from garlic sprouts.
Sommerburg et al. Reference Sommerburg, Keunen, Bird and van Kuijk56 measured zeaxanthin and other carotenoids in thirty-six commonly consumed fruits and vegetables but do not report concentrations, only ratios of lutein and zeaxanthin. Several reports agree that the fruits providing the largest amounts of zeaxanthin are orange peppers, oranges (both fruit and juice), honeydew melon, mango and peachesReference Khachik, Beecher and Smith44, Reference Sommerburg, Keunen, Bird and van Kuijk56 and there are particularly large amounts in persimmons (488 μg/100 g)50.
Meso-zeaxanthin content of chicken eggs in Mexico
One of the foods identified by Sommerburg et al. Reference Sommerburg, Keunen, Bird and van Kuijk56 and the USDA50 as being relatively rich in zeaxanthin was chicken eggs. This is because in many parts of the world, including the USA, the poultry industry uses xanthophylls for broiler and egg pigmentation. However, this is not an unnatural situation since when chickens are allowed to forage naturally, they will acquire lutein and zeaxanthin from grasses and other vegetation, to deposit in the yolkReference Schiedt, Leuenberger, Vecchi and Glintz49. In young birds the carotenoids are deposited in the flesh but with sexual maturity, the carotenoids are mobilised from the tissues and transferred to the reproductive organs and the eggs. In laying hens most of the absorbed carotenoids are transferred to the eggsReference Schiedt, Leuenberger, Vecchi and Glintz49.
Xanthophyll carotenoids in chicken eggs also contain a relatively high proportion of zeaxanthin where lutein:zeaxanthin ratios of about 1·4:1 have been reportedReference Handelman, Nightingale, Lichtenstein, Schaefer and Blumberg35, 50, Reference Sommerburg, Keunen, Bird and van Kuijk56. However, there is a wide variation in xanthophyll pigments in eggsReference Handelman, Nightingale, Lichtenstein, Schaefer and Blumberg35, Reference Surai, Macpherson, Speake and Sparks36, Reference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40, 50, Reference Leeson and Caston57, Reference Ollilainen, Heinonen, Linkola, Varo and Koivistoinen58, with values ranging from 1·450 to 41·540 μg/g egg yolk for zeaxanthin and 1·950 to 12957 μg/g egg yolk for lutein assuming a yolk size of 17 g. The relatively high zeaxanthin:lutein ratio in eggs suggests that the chicken specifically concentrates zeaxanthin in the egg, as the marigold extract fed currently to most laying hens generally contains < 10% zeaxanthin as would any grasses consumed in the wild. A maize feed would contribute a higher proportion of zeaxanthin than marigold extract or wild grasses, but the extent to which selective uptake of zeaxanthin occurs requires more information on the absolute amounts of zeaxanthin in the chicken feed.
The amount of pigment used in the feed of laying hens is determined by the density of the yellow or yellow-orange colour of the yolk that is preferred by the consumer. In the USA, the colour approximates 7–8 as measured by the Roche fanReference Vuilleumier59 whereas in Mexico consumers prefer a deeper colour, 11–13. Over the last 10 years, Industrial Organica SA (Monterrey, Mexico) has supplied pigment to 25–28% of the combined broiler and layer pigment market in Mexico (personal communication, José Torres, Industrial Organica SA 2006). The main pigment used for layers in Mexico is Yemix that comprises 70% xanthophyll concentrate (of which a half is MZ) and 30% capsanthin, although more recently the capsanthin has been replaced by lutein and some canthaxanthin. We recently measured the xanthophyll pigments in a lyophylised sample of Mexican chicken egg yolks (Table 4) obtained from birds that had been given feed containing 13–14 parts per million Yemix® of the newer composition. The analysis indicated that the xanthophyll concentration in the yolk was about 10 μg/g, containing approximately 10% MZ. The lutein:total zeaxanthin ratio was 1·7.
Xanthophyll composition of yolk lyophilisate from chickens fed Yemix®*
ppm, Parts per million.
* Yemix® (Industrial Orgánica SA, Monterrey, Mexico) contains 60–65% mixed zeaxanthin isomers (mainly meso-zeaxanthin), 25–30% lutein and a small amount of canthaxanthin and was fed to chickens at 13–14 ppm.
† The method used was the standard operating procedure employed in Industrial Orgánica SA (PEO-ACC-005, revision 01, 2 May 2005). In brief, samples of lyophylisate (about 2 g) were sonicated briefly in 30 ml of solvent mixture (hexane–acetone–ethyl alcohol–toluene, 100:70:60:70, by vol.) to suspend the sample and 2 ml 40% methanolic KOH was added in a 100 ml volumetric flask. The solution was mixed, left at 4°C overnight, heated at 56°C for 20 min, cooled to room temperature and the contents made up to 100 ml by the addition of 30 ml hexane and 10% aqueous anhydrous sodium sulfate. After vigorous mixing, the flask was again stood in darkness at 4°C overnight (about 20 h) to allow the supernatant fraction solution to clarify. Absorbance was then measured at 474 nm against a hexane blank. The composition of the carotenoids was then measured as described elsewhereReference Bone, Landrum, Cao, Howard and Thurnham45.
Mexico is the third largest consumer of eggs in the world. The annual per capita consumption of eggs increased from 13·1 kg in 1995 to 18·3 kg in 2002 and most of the eggs consumed in Mexico are supplied by the domestic marketReference Gallardo Nieto, Villamar, Ruiz Sanchez, Castillo Mangas and Guzman60. Assuming an egg weighs 60 gReference Leeson and Caston57, egg consumption in Mexico is approximately one egg/person per d. That is, in 2002, 25–28% of the Mexican population (26–29 million Mexicans) would have received about 170 μg (if the egg yolk is 17 g) xanthophyll pigments per d from eggs, of which about 34 μg was 3R,3′R-zeaxanthin, of which 35% was MZ. In the USA, eggs probably contribute little to the zeaxanthin intake of most AmericansReference Jacob and Miles61 as light-coloured yolks are preferred in the USA. Mexican Americans living in the USA are reported to consume 1·1–1·2 mg lutein/d across the ages 40–80 yearsReference Mares-Perlman, Fisher, Klein, Palta, Block, Millen and Wright28. If the average lutein:zeaxanthin ratio in food in the USA is 5:1Reference Hartmann, Thurmann, Spitzer, Schalch, Manner and Cohn30, the average intake of zeaxanthin is about 200 μg/d. Thus about 28 million Mexicans may have consumed about an additional 34 μg mixed zeaxanthin isomers compared with other Americans. While numerically the additional amount of xanthophyll pigment may be small, the three to four times better bioavailability of egg compared with vegetable xanthophylls may be equivalent to doubling the dietary intake of xanthophyll pigments, so reducing the potential risk of macular disease, but currently there is no evidence on the comparative risks of Mexicans to macular disease in the two countries available or of measurements of blood xanthophyll concentrations in Mexico. However, in a recent study, workers gave six eggs/week for 12 weeks to adult females (Table 2) and reported a significant increase in MPODReference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40. The xanthophyll content of the eggs in this study was double that found in the Mexican egg lyophylisate but the study shows the potential advantage of a daily intake of eggs.
Serum zeaxanthin and lutein concentrations, macular pigment optical density and age-related macular disease
The very high concentrations of lutein and the two stereoisomers of zeaxanthin in the macula has stimulated much speculation as to the function of these compoundsReference Bone, Landrum and Tarsis1, Reference Handelman, Dratz, Reay and Van Kuijk3, Reference Bone, Landrum, Hime, Cains and Zamor4. It is generally believed that the macular pigments by their presence in the photoreceptor axonsReference Snodderly, Auran and Delori16 may filter out blue light (about 400–500 nm) and shield posterior tissues such as the photoreceptor outer segment from the photo-oxidative damage caused by blue lightReference Bone, Landrum, Cao, Howard and Alvarez-Calderon62. The retina is vulnerable to oxidative damage for several reasons: constant exposure to light and high levels of oxygen, the lipid bilayer of the photoreceptor outer segments contain high concentrations of PUFA, and the retina and the retinal pigment epithelium have an abundance of photosensitisersReference O'Connell, Neelam, Nolan, Au Eong and Beatty6. The antioxidant activity and free-radical-scavenging properties of macular pigment may limit photo-oxidative damage caused by blue lightReference Handelman, Dratz, Reay and Van Kuijk3 and the higher concentration of the zeaxanthin stereoisomers than lutein in the macula may also relate to the more effective quenching activity of zeaxanthin than lutein for singlet oxygenReference Cantrell, McGarvey, Truscott, Rancan and Böhm63. It follows from these factors that maculae with high pigment densities should provide more protection from oxidative damage than that of maculae with less pigment, and that those individuals with poor pigment densities will be more at risk of damage. The risk of AMD may be a consequence of poor macular pigmentation and as the source of these pigments is from the diet, there is considerable interest in understanding the relationships between dietary intake and serum concentrations of the macular pigments and the xanthophyll content of the macula that is measured as MPODReference Nolan, Stack, O'Connell and Beatty64.
The Eye Disease Case–Control Study Group was among the first groups to show an inverse correlation between the risk of AMD and the combined concentrations of serum lutein and zeaxanthin in man65, Reference Seddon, Ajani and Sperduto66. The authors also found that a higher frequency of intake of spinach or collard greens was also associated with a substantially lower risk of AMD. The Eye Disease Case–Control Study Group evaluated the hypothesis that higher serum levels of micronutrients with antioxidant capabilities may be associated with decreased risk of neovascular AMD. The study compared serum levels of carotenoids, vitamins C and E and Se in approximately 421 cases and 615 control individuals with other eye diseases recruited in the same geographic areas in five centres. Micronutrients were classified by blood concentrations into three groups (low, medium and high). There were no individually significant effects for vitamin C, vitamin E or Se, but for individuals with carotenoid levels in the medium and high groups (except lycopene) the risk of AMD was statistically significantly lower65. For individuals with lutein or zeaxanthin, β-carotene, α-carotene and cryptoxanthin concentrations in the high percentile groups, OR of neovascular AMD ranged from 0·3 to 0·5 compared with those in the low percentile group.
More recently, another study was undertaken to investigate MPOD with respect to risk factors for AMD and dietary and serum concentrations of lutein and zeaxanthin in 828 healthy Caucasian Irish subjects aged 20–60 yearsReference Nolan, Stack, O'Connell and Beatty64, Reference Nolan, Stack, O'Donovan, Loane and Beatty67. The authors found a statistically significant age-related decline in MPOD, that current and past smokers had lower MPOD than never smokers, and subjects with a confirmed family history of AMD had lower MPOD than those with no known historyReference Nolan, Stack, O'Donovan, Loane and Beatty67. In addition, the relationships between MPOD, serum concentrations of lutein and zeaxanthin, and dietary intake of lutein and zeaxanthin were positive and statistically significant when analysed for the entire study group (r 0·136–0·303; P < 0·01 for all). However, in sub-group studies, for subjects with a clinically confirmed family history of AMD, subjects aged more than 53 years, and subjects with a BMI >27 kg/m2, there were positive and significant relationships between MPOD and serum concentrations of lutein but not with zeaxanthin. In current heavy cigarette smokers, there were no significant relationships between MPOD and serum concentrations of either lutein or zeaxanthin. Thus in those subjects at increased risk for AMD, the authors suggested the results indicated impaired ability to accumulate circulating concentrations of zeaxanthin and in current heavy smokers this extended to lutein as wellReference Nolan, Stack, O'Connell and Beatty64.
The fact that the Eye Disease Case–Control Study found the risk of macular disease to be inversely related to all the serum carotenoid concentrations except lycopene and not just lutein and zeaxanthin is understandable because high intakes of vegetables and fruits will tend to raise plasma concentrations of all the carotenoids. Lycopene, however, is different to the other carotenoids since there is really only one dietary source, that is tomatoes, and these fruits are used to make ketchup that is often consumed in large amounts and in separate meals from the other carotenoids. We also found plasma lycopene concentrations differed from the other carotenoids in not being lower in smokers than non-smokers in survey data from UK adultsReference Thurnham68. The carotenoids believed to be of greatest importance for macular pigmentation, however, are lutein and zeaxanthin, as the others are there in only small amountsReference Handelman, Snodderly, Adler, Russett and Dratz5.
Various workers have shown that MPOD is positively correlated with both dietary intakesReference Sickel19, Reference Curran-Celentano, Hammond, Ciulla, Cooper, Pratt and Danis27, Reference Nolan, Stack, O'Connell and Beatty64 and serum concentrationsReference Sickel19, Reference Ciulla, Curran-Celentano, Cooper, Hammond, Danis, Pratt, Ricardi and Filloon26, Reference Nolan, Stack, O'Connell and Beatty64, Reference Broekmans, Berendschot, Klopping-Ketelaars, de Vries, Goldbohm, Tijburg, Kardinaal and Van Poppel69, Reference Johnson, Hammond, Yeum, Qin, Wang, Castaneda, Snodderly and Russell70 of lutein and zeaxanthin. These relationships suggest that dietary intervention alone should influence MPOD. A number of workers have shown that by using relatively pure supplements of lutein and more recently zeaxanthinReference Landrum, Bone, Joa, Kilburn, Moore and Sprague12, Reference Bone, Landrum, Guerra and Ruiz13, Reference Bone, Landrum, Cao, Howard and Alvarez-Calderon71 it is possible to increase MPOD but food has also been used successfullyReference Hammond, Johnson, Russell, Krinsky, Yeum, Edwards and Snodderly14, Reference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40. Hammond et al. Reference Hammond, Johnson, Russell, Krinsky, Yeum, Edwards and Snodderly14 fed 60 g spinach (10·8 mg lutein and 0·3 mg zeaxanthin) to eleven adult subjects. Ten of the subjects also added 150 g maize (0·4 mg lutein, 0·3 mg zeaxanthin) and two other subjects were given only maize. The intervention lasted 15 weeks and the subjects ate the supplement with a meal or with a fat source. Eight subjects responded fully with significant increases in both serum lutein concentrations (33%) and MPOD (19%). The increase in both measurements was rapid (4 weeks) and MPOD was still elevated up to 9 months after treatment was discontinued. Two non-responders showed an increase in serum lutein concentrations but no change in MPOD and one non-responder had no change in either measurement. However, it emerged in the discussion of these results that the dietary intake of lutein and zeaxanthin of the non-responders before the study was four times higher than that of those who later responded and the concentrations of serum lutein and zeaxanthin were 17 and 52% higher at baseline respectively. As the baseline intake of the responders was approximately 2·3 mg xanthophyll carotenoids, the baseline intake of the non-responders will have been 8–9 mg of xanthophyll carotenoids, suggesting that the lack of response was due to the already high intake of xanthophyll carotenoids.
In another study using food, MPOD increased significantly in female adults who consumed six eggs/week for 12 weeksReference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40. Twenty-four subjects were allocated to three groups; egg I (lutein 167, zeaxanthin 166 μg/d), egg II (lutein 519, zeaxanthin 308 μg/d) or a pill containing sugar. There were significant increases in serum zeaxanthin in both eggs groups but lutein only increased in response to the egg I treatment (Table 2). Three subjects were dropped from the egg groups because of concern over their cholesterol. Of the remainder, MPOD increased in eight and failed to increase in five subjects. A possible reason for non-response may have been the significantly higher MPOD at baseline in the egg II group since the authors reported an inverse relationship between the change in MPOD and baseline MPOD.
Three important factors emerge from these studies: baseline vegetable intake and baseline MPOD are determinants of the potential response from a xanthophyll supplement and that the xanthophylls in eggs are much more effective in increasing MPOD than those in vegetables. The increase in plasma zeaxanthin concentrations in the egg study in both egg-treatment groups may be causally related to the increase in MPOD but alternatively the increase in the plasma zeaxanthin concentration may simply be a consequence of the lower baseline plasma zeaxanthin concentration than lutein.
Dietary zeaxanthin concentrations may have a greater influence on MPOD than we currently realise. Zeaxanthin is after all the major carotenoid in the maculaReference Bone, Landrum and Tarsis1 and low serum zeaxanthin concentrations inversely correlated with the risk of AMD in a recent study in SheffieldReference Gale, Hall, Phillips and Martyn72. A xanthophyll-rich diet, like spinach in the study of Hammond et al. Reference Hammond, Johnson, Russell, Krinsky, Yeum, Edwards and Snodderly14, also supplies zeaxanthin, and may fail to increase MPOD if plasma or tissue concentrations of zeaxanthin are already high in someone consuming an above average intake of vegetables. Very little is still known of the factors determining the conversion of lutein to MZ, and zeaxanthin may bind preferentially to the GSTP1-binding proteinReference Bhosale, Larson, Frederick, Southwick, Thulin and Bernstein15 and suppress the formation of or prevent uptake of newly formed MZ. That is, a negative-feedback mechanism may prevent excessive pigmentation of the retina. In this respect, Hammond and colleagues reported that two of the non-responders showed a slight decrease (11%; NS) in MPODReference Hammond, Johnson, Russell, Krinsky, Yeum, Edwards and Snodderly14. It may also be important to note that in the recent large Irish study, the authors noted that in subjects with a higher risk of AMD, there was a lack of correlation between MPOD and serum zeaxanthin concentrations which the authors suggested may indicate an impaired ability to accumulate circulating zeaxanthinReference Nolan, Stack, O'Connell and Beatty64.
The recent study where serum zeaxanthin concentrations were linked with a higher risk of AMD also found birth weight was positively associated with a risk of AMD. Birth records of 660 men and women born between 1922 and 1930 were examined and 392 of the subjects were contacted to do an ophthalmic examination. Of the subjects, 20·5% (forty-five men, thirty-three women) had signs of mostly early (17%) or late (3·7%) AMD. Subjects with AMD were significantly older than those without but there was no sex difference. After controlling for age, sex, serum zeaxanthin concentration, smoking and beer drinking etc, the surprising finding was that birth weight was positively related to the risk of AMDReference Hall, Gale, Syddall, Martyn and Phillips73. However, the workers also measured serum concentrations of lutein and zeaxanthin and found that the risk of early or late AMD was significantly higher in those with the lower concentrations of serum zeaxanthin. A slightly lower risk of AMD was obtained when lutein was examined separately or if lutein and zeaxanthin were combined, but neither of the latter results were significant (Table 5)Reference Gale, Hall, Phillips and Martyn72. The writer is not aware of any association between birth weights and serum xanthophyll concentrations in adults.
Age-related macular degeneration (AMD) and plasma zeaxanthin and lutein concentrations
IQR, interquartile range.
Several studies have shown that smokers have a higher risk of AMD than non-smokersReference Vingerling, Hofman, Grobbee and de Jong74, Reference Klein, Klein and Moss75 and lower MPOD than non-smokersReference Nolan, Stack, O'Donovan, Loane and Beatty67. In The Rotterdam Study, in subjects under 85 years, current smokers had a 6·6-fold higher risk of neovascular AMD and adjusting the results for atherosclerosis did not change the associationReference Vingerling, Hofman, Grobbee and de Jong74. In the Beaver Dam Study it was reported that men (but not women) who smoked greater amounts of cigarettes were more likely to develop early age-related maculopathy than men who smoked lessReference Klein, Klein and Moss75. The conclusion in both these studies was that there was a dose–response relationship between smoking and AMD but the mechanism by which smoking influenced the risk of AMD is not known.
We have previously shown that plasma lutein concentrations were depressed in smokers compared with non-smokersReference Thurnham68 in data from The Survey of British Adults in 1988–9. However, it seems unlikely that the lower MPOD in smokers are just a consequence of lower circulating concentrations of lutein and zeaxanthin since, as explained elsewhere, the absolute amount of xanthophylls in serum exceeds by several thousand-fold the absolute amount of xanthophylls in the macula. It seems more likely that other factors associated with smoking influence selective uptake mechanisms. The higher concentration of the zeaxanthin isomers relative to lutein in the macula of the eye and the different distribution of the isomers in the macula in comparison with serum suggests that specific uptake mechanisms for the different xanthophyll carotenoids must exist in the eye. In addition, studies in primates suggest that the uptake and assimilation of the macular carotenoids are biologically regulated by selective mechanisms, as comparative studies on the macular pigment concentrations in the left and right eyes of individual monkeys showed excellent agreement in both zeaxanthin (within 5%) and lutein (within 11%)Reference Handelman, Snodderly, Krinsky, Russett and Adler76. However, there were large differences in macular pigment concentrations between different monkeys (up to 4-fold for zeaxanthin), suggesting, as is observed in human subjects, that phenotype as well as diet and serum may all influence the pigmentation of macular tissue.
Differences between men and women in the relationships between MPOD and serum carotenoids indicate the importance of biological control of retinal uptake of the macular pigments. In a study on non-smoking American women (n 48) and men (n 40), there was no difference in the dietary intake of carotenoids or of the combined plasma concentrations of lutein and zeaxanthin, yet MPOD was 38% higher in men than women (P < 0·001)Reference Hammond, Curran-Celentano, Judd, Fuld, Krinsky, Wooten and Snodderly77. Furthermore, dietary intake of carotenoids and serum concentrations of lutein and zeaxanthin were both positively related to MPOD in men but only serum concentrations of the xanthophyll carotenoids and MPOD were related in the women. We also observed differences in the plasma responses to supplements of lutein (10·2 mg/d) and MZ (8 mg/d) between men and womenReference Thurnham, Tremel and Howard46. Following 21 d of supplementation, plasma responses of both lutein (P = 0·013) and MZ (P = 0·001) were higher in the women than the men but there was no difference in the case of 3R,3′R-zeaxanthin (1·2 mg/d; P = 0·95). Both groups appeared to have similar intakes of fruit and vegetables, and baseline concentrations of plasma lutein and 3R,3′R-zeaxanthin were not different between the sexes. Women are reported to have a higher risk of AMD than menReference Klein, Klein, Jensen and Meuer78, 79 but whether the higher plasma responses indicate a higher demand for the macular pigments in women than men is not yet known.
Smokers frequently display evidence of sub-clinical inflammationReference Das80, Reference Northrop-Clewes and Thurnham81 and inflammation in both smokers and non-smokers is inversely related to serum carotenoid concentrationsReference Kritchevsky, Bush, Pahor and Gross82, Reference Erlinger, Guallar, Miller, Stolzenberg-Solomon and Appel83. Seddon et al. Reference Seddon, Gensler, Milton, Klein and Rifia84 reported concentrations of the systemic inflammatory marker C-reactive protein to be an independent risk factor for AMD. Therefore an additional factor to consider in interpreting the reason for non-response to dietary supplemental carotenoids may also be inflammation. The relationship was explored in the participants of the Age-Related Eye Disease Study because C-reactive protein is elevated in CVD and some of the risk factors for the latter are known to be associated with AMD. Smoking is associated with an increased risk of AMDReference Vingerling, Hofman, Grobbee and de Jong74, Reference Klein, Klein and Moss75 and smokers often have elevated C-reactive protein (i.e. inflammation) and depressed serum xanthophyll concentrationsReference Kritchevsky, Bush, Pahor and Gross82, Reference Erlinger, Guallar, Miller, Stolzenberg-Solomon and Appel83. It is not known if inflammation explains the non-response in the studies of Hammond et al. Reference Hammond, Johnson, Russell, Krinsky, Yeum, Edwards and Snodderly14 and Wenzel et al. Reference Wenzel, Gerweck, Barbato, Nicolosi, Handelman and Curran-Celentano40. Individuals with known diseases were excluded but the subjects were aged 24–65 years and the prevalence of sub-clinical inflammation increases with age. But, if inflammation is an independent risk factor for AMD, inflammation markers should be included when workers explore relationships between the risk of AMD and serum carotenoid concentrations.
The recent study of factors influencing MPOD in healthy Irish individuals confirmed several of the points made regarding smoking and MPOD outlined above. MPOD and serum lutein concentrations were significantly lower in the heavy smokers (> twenty cigarettes/d) than both the light ( < twenty cigarettes/d) and non-smokers, although serum zeaxanthin concentrations were not different between the groupsReference Nolan, Stack, O'Donovan, Loane and Beatty67. In non-smokers and current light smokers, MPOD was positively correlated with both lutein and zeaxanthin concentrations in serum. However, in current heavy smokers there were no correlations with either xanthophyll in spite of serum lutein and MPOD being lowest in this groupReference Nolan, Stack, O'Connell and Beatty64. Inflammation is likely to be higher in the heavy than mild smokers or non-smokersReference Das80, thus the lack of correlations between MPOD and serum lutein or zeaxanthin in the heavy smokers may be due to inflammation-related disturbances in serum xanthophyll concentrations and/or interference with uptake mechanisms as suggested earlier.
Conclusions
A number of factors indicate that specific uptake mechanisms for the different xanthophyll carotenoids must exist in the eye. The recent report of a binding protein in optical tissue with similar binding capacity for the two zeaxanthin isomers but not for lutein is no doubt part of the explanation. The binding protein may respond differently in men and women and may be impaired by external factors such as inflammation. The relatively high binding capacity for MZ by this protein is surprising in view of the fact that MZ is not a normal component of the diet or serum in man. Whether MZ supplements to increase plasma concentrations of MZ will be accessible to this protein and will influence MPOD and eye health still needs to be determined.
The optimal plasma response obtained with long-term feeding of pure lutein or zeaxanthin is approximately 0·1 μmol/l per mg xanthophyll supplement and is achieved after approximately 21 d. Vegetable supplements of both lutein and zeaxanthin are both far less efficient in increasing plasma concentrations than the pure supplements but sometimes even the pure materials are poorly absorbed even when administered in the same vehicle. Plasma responses to xanthophyll supplements given as eggs were particularly high and in spite of their low content of xanthophyll carotenoids compared with vegetables, there was a significant increase in MPOD in adult female subjects given < one egg/d. Eggs are a particularly useful source of xanthophyll pigments as they naturally contain a relatively high proportion of zeaxanthin. Laying hens divert both endogenous and dietary xanthophylls to their eggs and the amount of pigment in the egg can be manipulated by the concentration of pigment in the feed. Currently a high proportion of the eggs consumed in Mexico will contain MZ obtained from their feed but the impact of this on eye health is not known. Lastly, long-term studies with pure lutein, mixtures of pure xanthophylls, lutein-rich foods and eggs have been shown to increase MPOD. Once increased, several studies have shown that MPOD remains high even though plasma xanthophyll concentrations fall relatively rapidly when intervention ceases. There is some evidence to suggest that a previously high dietary intake of lutein (and by proxy zeaxanthin) may optimise MPOD and prevent any further increase in macular pigment optical density with xanthophyll supplements. Such observations are important as they may help to quantify the amount of dietary xanthophyll needed for optimal macular pigment density, but much more work in this area is still needed.
