Hypertension increases the risk for CVD, stroke and kidney disease. Reduction of salt in the diet for the prevention and treatment of hypertension predates the availability of drug therapy for the treatment of hypertension. Exclusion of salt from the diet of hypertensives was recommended as early as 1905(Reference Grollman1). Allen and Sherrill recommended salt restriction for treatment of hypertension in 1905 and subsequently in 1922(Reference Grollman1). The dietary treatment of hypertension received a big boost with the introduction of the rice diet by Kempner in 1944(Reference Grollman1). Grollman documented that the low salt level of the diet was the primary determinant for the decrease in blood pressure. In the 1950s, it was recognized that in spite of the benefits of reducing salt intake on blood pressure levels, it was difficult to implement such a diet and there was substantial variation in response to reduced salt intake(Reference Meneely2). Furthermore, it was recognized that measurement of the dietary intake of Na was very difficult and that only 24 h urinary excretion provided a reasonable estimate of dietary intake(Reference Meneely2, Reference Meneely3). Dahl noted that the average daily US intake of salt in the 1950s was between 5 and 15 g. He estimated that human consumption only needed to be between 100 and 400 mg NaCl/d(Reference Dahl4).
Na is an essential nutrient in the human body used for maintaining cell homeostasis by regulating extracellular fluid. A small amount of natural Na is found in virtually all foods, with greater amounts added during processing or during the cooking process. On average, Americans consume much higher amounts than is physiologically needed. Minimum daily requirements to replace necessary losses have been estimated at 115 mg; however the recommended safe minimum level for adults is set at 500 mg to accommodate varying levels of activity and environmental conditions(Reference Denton5, Reference Stipanuk6). Excess dietary Na causes retention of Na and water, an increase in venous tone, peripheral resistance and central (but not total) blood volume, and higher blood pressure; it also increases left ventricular mass and cardiovascular morbidity(Reference Tuomilehto, Jousilahti, Rastenyte, Moltchanov, Tanskanen and Pietinen7–Reference Pinto9). For decades, there has been sufficient evidence from clinical trials demonstrating that non-pharmacological interventions including Na reduction are effective not only in decreasing blood pressure and preventing hypertension, but also in decreasing left ventricular mass(Reference Jula and Karanko10) and reducing cardiovascular morbidity(Reference Cook, Cutler, Obarzanek, Buring, Rexrode, Kumanyika, Appel and Whelton11–Reference Chobanian, Bakris and Black17).
The 2005 Dietary Guidelines for Americans and the Institute of Medicine recommended an Na intake of ≤2300 mg/d for ‘normal’ individuals and ≤1500 mg/d for individuals considered at increased risk (individuals with hypertension, blacks, middle-aged and older adults)(18, 19). Consuming Na in greater than recommended amounts is considered a risk factor for increasing blood pressure(Reference Elliott, Stamler, Nichols, Dyer, Stamler, Kesteloot and Marmot20–Reference Dyer, Elliott and Shipley23). Past trials comparing behavioural interventions have provided scientific evidence that lifestyle modifications such as reducing dietary Na intake to recommended levels can result in blood pressure reduction and prevention of hypertension(Reference Appel, Champagne and Harsha12–Reference Appel, Espeland, Easter, Wilson, Folmar and Lacy16, Reference Appel, Moore and Obarzanek24).
The Dietary Approaches to Stop Hypertension (DASH) trial was a landmark study for Na intervention in which participants were randomly assigned to one of three diets, each containing 3000 mg Na/d with a 500 mg daily discretionary Na allowance. Participants had a mean age of 44, 45 and 44 years for the control diet, the fruits and vegetables diet and the combination DASH diet, respectively(Reference Appel, Moore and Obarzanek24, Reference Karanja, Erlinger, Pao-Hwa, Miller and Bray25). The DASH diet is high in fruits, vegetables, low-fat dairy products, whole grains, poultry, fish and nuts. This diet is also low in saturated fats, red meat, sweets and sugar-containing beverages(Reference Karanja, Erlinger, Pao-Hwa, Miller and Bray25). All foods were provided for the 11-week intervention and participants were advised not to consume any ‘outside’ foods. All three groups experienced blood pressure reduction. The DASH diet group showed the greatest average reduction in systolic blood pressure (SBP; 6 mmHg) and a 3 mmHg reduction in diastolic blood pressure (DBP). This reduction was even greater when stratified for persons with stage 1 hypertension (average reduction in SBP and DBP of 11 mmHg and 6 mmHg, respectively)(Reference Appel, Moore and Obarzanek24).
Accurately capturing dietary Na intake from all sources poses a considerable challenge. Collecting dietary Na intake through food questionnaires is often riddled with under-reporting(Reference Moses26, Reference Espeland, Kumanyika, Wilson, Reboussin, Easter, Self, Robertson, Brown and McFarlane27). The preferred methodology involves the use of 24 h urinary Na excretion to obtain reliable values. Under normal conditions, approximately 93 % of dietary Na is excreted through the urine, with minimal losses through faeces and sweat. Daily Na retention is approximately 3·4 % or 7·4 mmol(Reference Sanchez-Castillo, Branch and James28, Reference Liu and Stamler29).
Salt reduction is often recommended for older individuals, especially those with hypertension, congestive heart failure and renal disease. There is, however, little objective evidence of the success of reducing salt intake, especially in the long term, as measured by changes in urinary Na excretion among free-living individuals. How much of a reduction in Na in the urine is obtainable in the community by providing nutritional counselling as compared with the reduction that may be obtained in a more rigorous, clinical trial setting? To answer this question, we examined the effects of implementing prevention guidelines for older individuals, the ‘10 Keys’™ to Healthy Aging. We evaluated the effects of the dietary recommendations to reduce salt intake among hypertensives. The study did not measure the effects on blood pressure nor compare individuals who did or did not receive the dietary advice. Clinical trials have clearly documented the benefits of salt reduction on blood pressure levels and therefore it would be redundant to repeat this, as such trials have also compared the effects of the dietary intervention v. no intervention. The present study was restricted to individuals living in their homes in the community. Many of the participants were either widows or widowers or had a disabled spouse who would be ineligible for the study.
Experimental methods
Study background
The 10 Keys to Healthy Aging, a core demonstration programme of the Center for Healthy Aging (a Centers for Disease Control and Prevention research centre) of the University of Pittsburgh, was a community study of the implementation of prevention programme guidelines in the elderly. This programme was designed to advocate preventive services in ten key areas and to monitor whether changes in health behaviours occurred. The keys are based on epidemiological, clinical and laboratory studies of the major diseases associated with morbidity, mortality and disability, and were developed in 2001 by experts in ageing from the University of Pittsburgh and around the country. The 10 Keys are:
1. Control SBP to <140 mmHg (optimal <120 mmHg);
2. Stop smoking;
3. Participate in cancer screenings;
4. Get regular immunizations;
5. Regulate blood glucose (fasting <100 mg/dl);
6. Lower LDL cholesterol to <100 mg/dl;
7. Be physically active;
8. Prevent bone loss and muscle weakness;
9. Maintain social contact;
10. Combat depression.
Men and women aged ≥65 years, with no significant disability, were the population of interest. The 2000 US Census Bureau reports that the city of McKeesport, Pennsylvania, which was the site of the community-based randomized trial, had 21 % of adults aged 65 and above. Residents reported a mean retirement income of $US 9355 along with a mean social security income of $US 10 904. Approximately 12·1 % of residents aged ≥65 years were living at or below the poverty level. The surrounding Allegheny County, Pennsylvania, has 18 % of adults aged 65 and above; the second highest proportion of older adults in a US urban county(30). All participants in this population were eligible for the above programme. Participants were then randomized to a more intensive lifestyle intervention, including both diet (i.e. low salt intake) and exercise, or to the 10 Keys alone.
Recruitment of participants
A detailed description of the recruitment process has been published elsewhere(Reference Newman, Bayles, McTigue, Williams, Robare, Taylor, Albert and Kuller31).
Potentially eligible volunteers underwent pre-screening in the form of a telephone interview. If eligible for the programme, informed consent was obtained and three successive health assessment visits were scheduled. The first visit scheduled was the home visit, in which health counsellors travelled to the individuals’ home to conduct the health assessment. These individuals were then scheduled for two ‘centre’ visits no more than one month apart. Transportation was arranged if needed or parking was validated. Health assessments ranged from 0·5 to 1·5 h in length. Following the completion of all health assessments, eligible and willing individuals were randomized by household into one of two groups: (i) Group 1, receiving a brief education and counselling intervention (BECI); or (ii) Group 2, receiving a brief education and counselling intervention that also included a physical activity programme for all participants and a dietary intervention for individuals with hypertension (BECI-plus). The advantage of two groups is that it makes it possible to compare a brief and less costly intervention (Group 1) with a more costly and intensive one (Group 2). Health status and physical function of all volunteers in both groups were reassessed bi-annually for 24 months.
Interventions
All participants received the same brief education and counselling intervention component designed to educate about the 10 Keys, to encourage adherence to age-appropriate screening procedures and vaccinations, and to work towards the reduction of specific health risk factors. As part of this intervention, each participant was given a ‘Prevention in Practice Report’ which summarized his/her status regarding each of the 10 Keys goals (Fig. 1). As the study continued, the 10 Keys goals were modified slightly to reflect changing preventive standards for serum glucose or lipid levels(Reference Genuth, Alberti and Bennett32, 33).
Prevention in Practice Report
Each participant met individually with a health counsellor every six months to review his/her Prevention in Practice Report. They identified the Keys that required attention and developed an action plan, including strategies such as knowledge acquisition, skills development, social support, self-monitoring and relapse prevention. This plan was discussed and modified via follow-up telephone calls with one of the study’s health counsellors at least every three months. Participants were encouraged to take the Prevention in Practice Report to their physicians and solicit their support in addressing these goals. Monthly calls were made to participants with concerning (but non-emergent) findings such as elevated blood pressure (SBP > 160 mmHg)(Reference Chobanian, Bakris and Black34) or blood glucose (>130 mg/dl)(35), a positive depression screening score (CESD > 16)(36), an absence of physical activity(Reference Nelson, Rejeski, Blair, Duncan, Judge, King, Macera and Castaneda-Sceppa37) or any current smoking behaviour. These calls helped ensure that appropriate medical follow-up was sought, provided support for behaviour change, and disseminated information on community resources that could potentially assist with recommended behaviour changes. Participants without a primary care physician were encouraged to make a physician appointment, and were given physician names and contact information from the local medical society and hospital. Those with difficulty obtaining or adhering to prescribed medications were provided information on prescription medication benefits and adherence strategies. Counselling calls continued on a monthly basis until active issues were resolved, and then reduced to approximately every three months for the remainder of the programme.
Individuals randomized to BECI-plus also received a physical activity intervention (the Key to Life Exercise Intervention) and, for those with a history of hypertension, a dietary Na nutritional intervention (the Key to Life Nutrition Program). For purposes of the current paper we discuss the Key to Life Nutrition Program only.
The Key to Life Nutrition Program was a 10-week, intensive dietary intervention, based on the DASH trials(Reference Sacks, Svetkey and Vollmer15, Reference Appel, Moore and Obarzanek24), which aimed to translate Na reduction approaches proved in randomized controlled trails into a community setting. The programme was limited to hypertensives only in the healthy lifestyle arm (Group 2). This intervention focused on educating individuals to reduce dietary Na by increasing the intake of fruits and vegetables, low-fat dairy products and whole grains, and decreasing the intake of processed foods and salt. This education was delivered in group sessions, facilitated by a registered dietitian. Weight loss was recommended only when BMI was ≥30 kg/m2.
The nutrition counselling was firmly grounded in behaviour theory, specifically behaviour modification and social learning theory(Reference Bandura, Adams and Beyer38–Reference Botelho and Skinner40). Participants were introduced to numerous self-management approaches such as self-monitoring, food label reading, goal setting, budgeting and problem solving. After ten initial weekly sessions, monthly follow-up sessions focused on maintaining motivation and adherence.
All interventions were conducted by health counsellors with at least a bachelor’s level training in health education, nutrition or exercise science, with standardized training in research assessment methods and behaviour change techniques(Reference Botelho and Skinner40). Additional health counsellor training in behaviour change methodology was provided by the University of Pittsburgh faculty affiliated with the project.
Target population
Hypertension was defined as SBP ≥ 140 mmHg or on blood pressure medications.
Assessment of dietary sodium
Those who agreed to participate were asked to collect a 24 h urine specimen at baseline and at successive centre visits (6 and 12 months). Efforts were taken to minimize participant burden associated with urine collection. To help improve completeness of the 24 h urine, volunteers were contacted and instructions for collection were reviewed.
While multiple collections over several days will improve the accuracy and reliability of urinary Na measures, data for the present study are based upon a single determination owing to the feasibility of expecting older adults to collect multiple 24 h urines for a community-based intervention.
A separate analysis was done using data from a sub-sample of our hypertensive group, which included seventy-seven participants who had a 24 h urine collection volume of ≥1000 ml. We chose 1000 ml since this represents a more normal kidney output for adults aged ≥65 years. The mean urine volume at baseline was 1956 ml compared with 1985 ml at the 12-month follow-up visit. In this sub-sample, comparison was done between baseline and 12-month follow-up data.
Analyses
Twenty-four hour urinary Na excretion was measured by Quest Diagnostics Incorporated (Madison, NJ, USA) using ion-specific electrode methodology. Specimen results were reported as millimoles, which were then converted to milligrams of Na. This conversion was calculated as 1 mmol = 23 mg dietary Na. Matched paired t tests were run using the SPSS statistical software package version 15·0 (SPSS Inc., Chicago, IL, USA) to assess differences in urinary Na at baseline and consecutive follow-ups.
Results
Baseline characteristics for participants are reported in Table 1. A total of 115 participants who met the established criteria for hypertension at baseline were randomized to the healthy lifestyle group (Group 2) and completed the 24 h urine collections. Average age was 75·1 (sd 5·3) years.
Baseline characteristics for persons with hypertensionFootnote * in the lifestyle and nutrition intervention group (n 115): University of Pittsburgh Center for Healthy Aging, Key to Life Nutrition Program
* Mean age of the participants was 75·1 (sd 5·3) years.
Table 2 reports 24 h urinary Na excretion in persons with hypertension at baseline, 6 months and 12 months. At baseline, mean urinary Na was 3128 mg/d (136 mmol/d). Comparing baseline and 6-month follow-up tests (103 matched pairs), the mean urinary Na dropped to 2990 mg/d (130 mmol/d) at the 6-month follow up. This 138 mg/d (6 mmol/d; 95 % CI −5·74, 8·10 mmol/d) reduction was not statistically significant (P = 0·30). When comparing baseline results with the 12-month follow-up (ninety matched pairs), there was a 299 mg/d (13 mmol/d; 95 % CI 1·16, 25·40 mmol/d) reduction in urinary Na excretion. This observed decrease was significant (P = 0·03). Mean urinary Na was reduced to 2875 mg/d (125 mmol/d).
Urinary sodium excretion in persons with hypertension in the lifestyle and nutrition intervention group: University of Pittsburgh Center for Healthy Aging, Key to Life Nutrition Program
*P value calculated using a matched paired t test, indicating statistical significance of the change in urinary Na from baseline to each of the follow-up points.
Table 3 refers to the sub-sample of our hypertensive group, and compares seventy-seven individuals at baseline and 12-month follow-up who had a 24 h urine collection volume of ≥1000 ml. The mean urine volume at baseline was 1956 ml compared with 1985 ml at the 12-month follow-up visit. When comparing urinary Na excretion at baseline and 12-month follow-up using a matched paired t test, mean urinary Na was 3220 mg/d (140 mmol/d) at baseline and decreased to 2875 mg/d (125 mmol/d) at the 12-month follow-up. This decrease of 345 mg/d (15 mmol/d; 95 % CI 3·02, 27·76 mmol/d) in urinary Na excretion was significant (P = 0·02).
Urinary sodium excretion in a sub-sample of the hypertensive group with 24 h urine volume ≥1000 ml: University of Pittsburgh Center for Healthy Aging, Key to Life Nutrition Program
*P value calculated using a matched paired t test, indicating statistical significance of the change in urinary Na from baseline to each of the follow-up points.
Discussion
In our demonstration programme changes in 24 h urinary Na excretion that occurred in persons with hypertension assigned to the Key to Life Nutrition Program intervention were significant, but fell short of the Institute of Medicine and the 2005 Dietary Guidelines recommendations (1500 mg Na/d) for this population. Our results reiterate how difficult it is to translate these recommended actions into community programmes. It is very unlikely that meeting Na reduction guidelines (i.e. 1500 mg/d) is obtainable, especially among at-risk older individuals with hypertension(18).
Numerous trials have demonstrated the beneficial effects and importance of reducing dietary Na to reduce blood pressure and ultimately heart disease and stroke(Reference Appel, Champagne and Harsha12–Reference Appel, Espeland, Easter, Wilson, Folmar and Lacy16). Past Na reduction trials (Table 4) have concluded that it is possible to use non-pharmacological approaches to reduce blood pressure and prevent hypertension in older adults.
Comparison of long-term sodium reduction trials
The DASH trial results described above (average reductions in SBP and DBP of 6 mmHg and 3 mmHg, respectively; even greater when stratified for persons with stage 1 hypertension: average reductions in SBP and DBP of 11 mmHg and 6 mmHg, respectively(Reference Appel, Moore and Obarzanek24)) sparked interest to investigate further how the DASH diet could be utilized for blood pressure control and hypertension prevention, and led to the DASH-Sodium trial, a randomized controlled trial that assessed the effects of multiple Na levels on hypertensive adults. Mean age was 47 years for the DASH diet and 49 years for the control diet. Utilizing a crossover design participants received controlled feedings with specific Na levels of 3000, 2400 and 1500 mg/d for 30 d. Results showed blood pressure reduction at all of the Na levels, but the most effective group was the DASH diet combined with Na reduction to 1500 mg/d. This group had an average reduction of 8·9 mmHg/4·5 mmHg for SBP/DBP. The reduction was greatest in persons with hypertension, who showed a reduction of 11·5 mmHg/5·7 mmHg for SBP/DBP. Persons considered normotensive also had a reduction of 7·1 mmHg/3·7 mmHg for SBP/DBP(Reference Sacks, Svetkey and Vollmer15).
In the PREMIER multi-centre clinical trial, adults with a mean age of 50 years and above-optimal blood pressure were selected to one of three lifestyle interventions. This trial assessed if a randomized controlled trial intervention could be translated with the same outcomes to the ‘real world’. Blood pressure reductions observed in the DASH diet group for SBP/DBP were 11·1 mmHg/5·1 mmHg, respectively. After group stratification (hypertensives and non-hypertensives) reductions were 14·2 mmHg/7·4 mmHg and 9·2 mmHg/5·8 mmHg, respectively(Reference Appel, Champagne and Harsha12). At 18 months, 24 h urinary Na excretion decreased by 5·6 mmol in the group given advice only, by 18·4 mmol in the group given traditional dietary recommendations for blood pressure lowering including lower Na intake and by 24 mmol in the group following the traditional low-salt plus DASH diet. There was a substantial increase in urinary Na between 6 and 18 months in the PREMIER trial. These results of the PREMIER trial are not very different from those in the current study, i.e. 15 mmol in the current study (Table 3), 18·4 mmol in the traditional low-salt diet in PREMIER and 24 mmol in the traditional low-salt plus DASH diet(Reference Elmer, Obarzanek and Vollmer41).
The mean age of participants in the Trial of Non-pharmacologic Interventions in the Elderly (TONE) was 66·5 years. The intervention assessed if a non-pharmacological intervention could take the place of antihypertensive medication. Na reduction goals for the intervention were 80 mmol (1840 mg) or less and were assessed through a 24 h urinary Na analysis. Long-term results (36 months) for this trial demonstrated the effectiveness and sustainability of Na reduction on blood pressure, with a reduction of 4·3 mmHg/2·0 mmHg for SBP/DBP. The results also focused attention on the fact that non-pharmacological therapy can be used to control hypertension in older adults without the need for medication(Reference Appel, Espeland, Easter, Wilson, Folmar and Lacy16).
In the Treatment of Mild Hypertension Study (TOMHS), the mean age of the participants was 54 years. The study concluded that lifestyle interventions along with pharmacological therapy in persons with stage 1 hypertension can be effective in reducing blood pressure. The long-term result for the lifestyle intervention group was a reduction of 10·6 mmHg/8·1 mmHg in SBP/DBP. This group also reported long-term success with 74 %, 70 % and 59 % of participants at follow-up years 2, 3 and 4, respectively, maintaining blood pressure control without the use of medications(Reference Elmer, Grimm and Laing14).
The Trials of Hypertension Prevention (TOHP I and II) demonstrated that blood pressure can be significantly reduced through Na reduction and weight loss. TOHP Phase I tested the feasibility and efficacy of multiple lifestyle interventions. Results from this trial spurred greater interest and TOHP Phase II tested the efficacy of two non-pharmacological interventions, weight loss, Na reduction and their combination, to decrease blood pressure. Mean age was 44 years, and the results of Phase II showed that early interventions alone and in combination were effective in decreasing blood pressure in the long term (48 months)(13).
It has recently been reported among participants randomized in the TOHP I (n 744) and TOHP II (n 2382) low-salt diet v. control that there was a 25 % reduction in cardiovascular events in the low-Na arm: relative risk = 0·75 (95 % CI 0·57, 0·99), P = 0·04. These results were not affected by weight change. Na reduction may have reduced the long-term risks of cardiovascular events in that study(Reference Cook, Cutler, Obarzanek, Buring, Rexrode, Kumanyika, Appel and Whelton11).
Study limitations
It is very difficult to reduce the salt intake of individuals by counselling alone, as described in the present study as well as from clinical trials. For example, at 18 months in the PREMIER trial, much of the reduction in salt intake was lost. The TOHP trial had only a modest effect on salt reduction although possibly even this small reduction was associated with a long-term reduction in cardiovascular events. The basic problem is the large amount of salt in the food supply, especially added to many foods. Individuals do not recognize the large amount of salt in processed food, which makes it very difficult to reduce their salt intake. A better approach is probably to treat excess salt as a common source epidemic and to reduce the amount of Na in the food chain. There would still be substantial within-individual variations in response to decreases in salt in the diet in relation to lowering of blood pressure and perhaps cardiovascular risk. Furthermore, other dietary approaches such as in the DASH diet, i.e. high-K diet, changes in protein and fat, can also affect blood pressure and enhance the low Na intake. As noted, Dahl has estimated that only about 0·5 g of salt is needed in the diet(Reference Dahl4). Risks of too low salt intake are probably extremely small.
The Food Standards Agency (similar to the US Food and Drug Administration) in the UK embarked on a major public health campaign to encourage food manufacturers to reduce Na levels in 2003. Presently reductions can be observed across the board. The Federation of Bakers has reduced salt levels by as much as 30 %, and the Association of Cereal Manufacturers reported a 33 % reduction in salt levels between 1998 and 2005. The Food and Drink Federation have reduced the salt content of soups and sauces by 30 %. Manufactures such as Heinz and Kraft have reduced salt in manufacturing; Heinz claims to have implemented a reduction of 11–18 % and Kraft has reduced Na in snack products by a third. The Food Standards Agency published revised salt reduction targets for 2012 in May 2009. The new targets focus on eighty categories of foods and are more challenging than the previous 2010 targets to reduce salt intake to 6 g daily(42).
Conclusion
By imposing more strict policy legislation and with the gradual reduction of Na in food manufacturing, the population would be able to slowly decrease its acquired taste for salt, without altering the taste of foods(Reference Bertino, Beauchamp and Engelman43). This would allow long-term reductions in Na, decreasing the risk for age-related blood pressure increase or hypertension and reducing strokes and CVD morbidity and mortality(Reference Chobanian, Bakris and Black17).
In contrast, in the USA between 1994 and 2004, despite manufacturing industry attempts at Na reduction, the average Na content in foods increased by 6 %(Reference Jacobson44). Without truly aggressive public health efforts, support from the food industry and health policy changes, the levels of Na reduction needed to help reduce blood pressure will be out of reach in the USA.
Recently, the American Medical Association and other agencies have recommended a reduction of Na in the food chain. Efforts are being made in several states in the USA also to mandate a reduction in salt in the food chain.
The large amount of salt added during the manufacturing process of foods has been implicated as a factor contributing to the difficulty of maintaining a long-term dietary Na intake reduction of 100 mmol/2300 mg or even 50 mmol/∼1200 mg per day for at-risk populations(Reference Kuller45). This decrease is not feasible because of the large amount of salt added during the manufacturing process of foods. The above studies show it is possible to reduce Na intake in a controlled environment, but our study showed the increased difficulty of reducing dietary Na intake in older adults in the real world.
Acknowledgements
Sources of funding: The study was funded by the Centers for Disease Control and Prevention Cooperative Agreement U48 DP000025-04, ‘Health Promotion and Disease Prevention Research Center’. Conflicts of interest: None declared. Author contributions: J.F.R. and L.H.K. conceived the study hypothesis. J.F.R. and R.B. were responsible for data analysis. J.F.R. wrote the manuscript. N.C.M., C.M.B., K.W., A.B.N., M.T.L., K.M. and S.M.A. edited the manuscript. The manuscript was revised by J.F.R. Final approval of the manuscript was given by L.H.K. Acknowledgements: The Center for Healthy Aging would like to thank all of its volunteers, partner organizations, as well as the Center for Healthy Aging staff and students, for their assistance and support. Ethical approval: 010866, The Center for Healthy Aging, University of Pittsburgh, Institutional Research Board.
