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We show that if $\Gamma $ is a point group of $\mathbb {R}^{k+1}$ of order two for some $k\geq 2$ and $\mathcal {S}$ is a k-pseudomanifold which has a free automorphism of order two, then either $\mathcal {S}$ has a $\Gamma $-symmetric infinitesimally rigid realisation in ${\mathbb R}^{k+1}$ or $k=2$ and $\Gamma $ is a half-turn rotation group. This verifies a conjecture made by Klee, Nevo, Novik and Zheng for the case when $\Gamma $ is a point-inversion group. Our result implies that Stanley’s lower bound theorem for centrally symmetric polytopes extends to pseudomanifolds with a free simplicial automorphism of order 2, thus verifying (the inequality part of) another conjecture of Klee, Nevo, Novik and Zheng. Both results actually apply to a much larger class of simplicial complexes – namely, the circuits of the simplicial matroid. The proof of our rigidity result adapts earlier ideas of Fogelsanger to the setting of symmetric simplicial complexes.
X-ray micro-computed tomography (μCT) is a technique which can obtain three-dimensional images of a sample, including its internal structure, without the need for destructive sectioning. Here, we review the capability of the technique and examine its potential to provide novel insights into the lifestyles of parasites embedded within host tissue. The current capabilities and limitations of the technology in producing contrast in soft tissues are discussed, as well as the potential solutions for parasitologists looking to apply this technique. We present example images of the mouse whipworm Trichuris muris and discuss the application of μCT to provide unique insights into parasite behaviour and pathology, which are inaccessible to other imaging modalities.
Stimulation of the central cholinergic system results in generalized epileptic seizures. The goal of this study was to map the epileptogenic effects of the cholinergic agonist, carbachol injected into different sites of the basal forebrain and diencephalon of the rat brain.
Methods:
Carbachol was injected directly into the brain in a dose of 1 or 3 (jg. Seizures were assessed behaviourally on a five-stage scale with electroencephalographic controls. Seizures at stage 1 were the least severe and those at stage 5 the most severe.
Results:
Injections of high dose carbachol (3 (jg) induced seizures from 40% of all injected brain sites. Injections of low dose carbachol (1 ug) or isotonic saline into the same brain sites did not cause any behavioural or electrographic seizures. The majority of sites (84%) producing generalized seizures (stage 5) were concentrated in or around the zona incerta.
Conclusions:
Within the anatomical limits of the study, the zona incerta is the area most sensitive to carbachol-induced generalized seizures.
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