Industrial development is rapidly altering ecosystems, with consequences for species that are culturally and ecologically important. Indigenous researchers with Swan River First Nation (SRFN) co-produced this research with Western scientists to examine how landscape change influences mammals within their traditional territory in the boreal foothills of Alberta (Canada). Community-identified concerns about declining mammal populations guided the study’s objectives, spatial focus and hypotheses and informed interpretation of the results. Using remote camera traps, we modelled species-specific responses to natural and anthropogenic landscape features. We applied generalized linear models to evaluate competing hypotheses for species occurrence–disturbance relationships. While some species–disturbance relationships matched community observations and patterns from neighbouring regions, others differed. Notably, and consistent with SRFN observations, moose (Alces alces) occurrence declined with increasing road and seismic line density, probably overriding positive effects of patch features. Integrating Indigenous knowledge with the study design and interpretation clarified potential mechanisms underlying declines and strengthened inference for management. These findings demonstrate the value of braiding Indigenous knowledge with ecological data to support conservation and management in rapidly changing landscapes.