Understanding the barriers to women’s employment and implementing inclusive initiatives in STEM workplaces is a critical global challenge. This systematic literature review applied a PRISMA-guided protocol to screen and critically appraise 44 empirical publications across four multidisciplinary databases. This review produced a structured analysis of the nature of barriers, their outcomes, and inclusive organisational initiatives for women in STEM workplaces. The study identifies intersecting barriers – bias-related, stereotype threat, culture, and structural – that hinder women’s representation and career progression. Inclusive initiatives, such as mentorship, stereotype reduction, equitable policies, and transparent promotion pathways, are explored as solutions; however, there are gaps in measuring their long-term efficacy and incorporating cross-cultural and intersectional perspectives. The findings underscore the need for robust theoretical frameworks and empirical research to promote equity and inclusion, thereby unlocking the full potential of women in STEM.