Impeller mowing conditioners are commonly used to speed up the drying process on the field, making forage preservation (haying, ensiling) less dependent on weather conditions. However, the effects of this technique on the nutritive value of the forage and dairy cows' responses have not been investigated yet. Each half of two fields of grass-dominated swards, first regrowth, was cut either with or without the use of an impeller mowing conditioner (experimental hay and control hay, respectively). Ceteris paribus conditions were guaranteed by the same cutting and wilting times (roughly 48 h), number of teddings, field pickup technique and barn-drying method. At the beginning of the feeding trial, 19 lactating Holstein cows were allocated to one of two groups, one control (nine cows) and one experimental group (10 cows) and were fed the respective forage plus a fixed amount of concentrate [3.6 kg d−1; dry matter (DM) basis]. After a 14-d adaptation period, data were collected over 21 consecutive days. Covariate data of cows were collected prior to the experimental feeding period, over a time span of 9 d, and included in the statistical model. Results revealed that control and experimental hay showed significant (P < 0.05) differences in the nutrient profile. However, the magnitude of these differences was not enough to affect intakes of hay (18.4 ± 0.29 kg DM d−1), total dietary energy or chewing activity, but did lead to a decreased intake of water-soluble carbohydrates and an increased crude protein intake, thus affecting ruminal nitrogen balance (P < 0.01). This resulted in a higher milk urea content [23.3 vs 17.9 mg (100 mL)−1; P < 0.01] in cows fed the experimental hay, whereas other milk performance parameters remained unaffected. In conclusion, the use of the impeller mowing conditioner did not affect the overall forage utilization by cows when the diet contained about 16% concentrate (DM basis). As this is the first study dealing with the effects of an impeller mowing conditioner on cows' responses, future research should consider investigating the effects of mowing conditioners when cows are fed only forage or diets with lower concentrate amounts.