Population control of the house mouse (Mus musculus), Norway rat (Rattus norvegicus) and black rat (Rattus rattus) is common practice worldwide. Our objective was to assess the impact on animal welfare of lethal and non-lethal control methods, including three dispatch methods. We used the Sharp and Saunders welfare assessment model with eight experts scoring eleven control methods and three dispatch methods used on the three species. We presumed the methods were performed as prescribed, only taking into account the effect on the target animal (and not, for example, on non-target catches). We did not assess population control efficacy of the methods. Methods considered to induce the least suffering to the target animal were captive-bolt traps, electrocution traps and cervical dislocation, while those with the greatest impact were anticoagulants, cholecalciferol and deprivation. Experts indicated considerable uncertainty regarding their evaluation of certain methods, which emphasises the need for further scientific research. In particular, the impact of hydrogen cyanide, chloralose and aluminium phosphide on animal welfare ought to be investigated. The experts also stressed the need to improve Standard Operating Procedures and to incorporate animal welfare assessments in Integrated Pest Management (IPM). The results of our study can help laypeople, professionals, regulatory agencies and legislators making well-informed decisions as to which methods to use when controlling commensal rodents.

The present study aimed to investigate the relationship between shooting angle to the head and animal welfare outcomes in the hunt of young harp seals (Pagophilus groenlandicus). The study population consisted of young harp seals belonging to the Greenland Sea harp seal population. A sample of 171, 2–7 weeks old, weaned harp seals of both sexes were included. The study was conducted as an open, randomised parallel group designed trial during the regular hunt. The animals were allocated into four groups, A–D, according to the observed shooting angle to the head, defined as the angle between the direction of the shot and the longitudinal axis of the animal’s head: (A) directly from the front; (B) obliquely from the front; (C) directly from the side; and (D) obliquely or directly from behind. Instantaneous death rate (IDR) and time to death (TTD) were the main variables. The mean IDR differed significantly between groups and was highest in group B (96.8%) and lowest in group C (66.7%). For all groups combined it was 84.2%. The mean TTD for seals not rendered instantaneously unconscious or dead (n = 27) differed significantly between groups and was shortest in group A (16 s) and longest in group C (85 s). However, the number of animals included in the TTD analysis was limited. In conclusion, based on the significantly higher IDR, the shooting angle obliquely from the front is recommended to help achieve the best animal welfare outcomes during the hunt of young harp seals.

In the UK and the Republic of Ireland, Official Veterinarians (OVs) are employed by the Food Standards Agency and the Food Safety Authority, respectively, as legal authorities for both animal welfare and food safety. However, little is known about job satisfaction in this profession which has the potential to impact professionals’ well-being and performance. Moreover, despite animal welfare issues being a reality that OVs witness, we do not yet understand how OVs perceive these issues at slaughter or whether this impacts job satisfaction. We assessed OVs’ perceptions on job satisfaction and views on welfare at slaughter across the UK and ROI, through an online questionnaire with 113 participants, which included socio-demographic information of participants and questions or statements about different aspects of job satisfaction and animal welfare issues at slaughter. While most OVs committed to their work they reported issues that may compromise job satisfaction, such as often experiencing loneliness at work, threatening situations and sleep disorders. Moreover, job satisfaction was often impacted by animal welfare incidents, and conflicts with food business operators were considered one of the greatest barriers to improving welfare at slaughter. There is also the likelihood of professionals’ individual ethical values being challenged since OVs are virtually certain to witness religious slaughter yet disagree with this practice to the extent that they consider it should be banned. We reveal significant challenges associated with the role of OV that justify creation of a support network to assist and safeguard this profession, as well as animal welfare at slaughter.

Minimising suffering is an ethical and legal requirement in animal research. This is particularly relevant for research on animal models of sepsis and septic shock, which show rapid progression towards severe stages and death. Specific and reliable criteria signalling non-recovery points can be used as humane endpoints, beyond which a study cannot be allowed to progress, thus preventing avoidable suffering. Body temperature is a key indicator for assessing animal health and welfare and has been suggested to have potential for monitoring the status of mouse models of sepsis. In this study, we monitored temperature variations using contactless methods – thermal imaging and subcutaneously implanted PIT tags – in a surgical model of sepsis by caecal ligation and puncture (CLP). We monitored body temperature variation following mid-grade CLP, high-grade CLP and sham surgery. All mice (Mus musculus) were monitored four times per day in the high-grade CLP model and three times per day in the mid-grade CLP model by both PIT tag readout and infrared thermography for ten days post-surgery, or until animals reached a predefined humane endpoint. Thermal data were compared with the clinical score and weight loss threshold used at our facility. Mean body surface temperature (MBST) assessed by thermal imaging and subcutaneous temperature (SCT) measured by PIT tags correlated, albeit not strongly. Moreover, while MBST does not appear to be a reliable predictor of non-recovery stages, SCT showed promise in this regard, even surpassing the widely used weight loss criterion, particularly for the high-grade CLP model of induced sepsis.

At least 200 billion black soldier fly (Hermetia illucens) larvae (BSFL) are reared each year as food and feed, and the insect farming industry is projected to grow rapidly. Despite interest by consumers, producers, and legislators, no empirical evidence exists to guide producers in practicing humane – or instantaneous – slaughter for these novel mini-livestock. BSFL may be slaughtered via freezing, boiling, grinding, or other methods; however standard operating procedures (SOPs) and equipment design may affect the likelihood of instantaneous death using these methods. We tested how larval body size and particle size plate hole diameter affect the likelihood of instantaneous death for black soldier fly larvae that are slaughtered using a standard meat grinder. Larval body size did not affect the likelihood of instantaneous death for larvae that are 106–175 mg in mass. However, particle size plate hole diameter had a significant effect on the likelihood of instantaneous death, with only 54% of larvae experiencing an instant death when using the largest particle size plate (12-mm hole diameter) compared to 84% using the smallest particle size plate (2.55 mm). However, a higher percentage of instantaneous death (up to 99%) could be achieved by reducing the proportion of larvae that become stuck in the machine. We conclude by outlining specific recommendations to support producers in achieving a 99% instantaneous death rate through specific SOPs to be used with similarly designed machines. We also develop a protocol for producers that wish to test their own grinding SOPs.

An estimated 0.9 to 2.5 trillion individual wild finfish, destined for human or animal consumption, are captured globally each year by commercial fisheries. The majority of wild-caught fish die either from asphyxiation or evisceration; slaughter methods considered inhumane. Humane stunning or stun/killing may improve the welfare of wild-caught fish at the time of slaughter by rendering fish immediately insensible to pain, stress and suffering. Reducing fish stress during slaughter may also improve product quality. This study systematically mapped and narratively evaluated scientific research regarding humane stunning or stun/killing of wild finfish caught for food and feed on a commercial scale. Multiple knowledge gaps were identified: Key humane stunning parameters have only been scientifically tested, in terms of fish welfare and/or flesh quality, for a minority of wild-caught fish species and stunning methods. More species-specific protocols and use of brain activity measurements to accurately assess state of consciousness on application of the stunning method are required. More scientific testing of stunning methods in commercial wild-capture settings is needed to validate findings of controlled laboratory studies and ensure the methods are practical in fisheries. Research is needed to better understand the potential economic benefits of humane stunning for fishermen. From a donor perspective this review highlights the need to support more primary research into the feasibility and implementation of humane stunning or stun/killing in wild-capture fisheries.

Commercial cattle slaughter operations have shown an increasing trend towards automation, with the aim being to improve animal welfare, product quality and efficiency. Several cattle slaughter plants have introduced mechanical rump pushers (RP) prior to the entrance of the stun box to reduce human-animal interaction and facilitate a smoother transition from the raceway to stun box. Presently, there are no data regarding the use of RPs in commercial slaughter environments operating at 40 cattle per hour. Therefore, this study observed normal operations at a UK slaughter plant, which has an RP installed, and assessed the level of coercion required to enter the RP, the use of the RP, cattle behaviour inside the RP and carcase bruising. The RP was used on 267 of the 815 cattle observed (32.8%) and was more likely to be used on dairy cattle and those who received a higher coercion score when entering the RP. Overall, 60 cattle (7.4%) required the highest coercion score and four (0.49%) required the use of the electric goad. Inside the RP, eleven animals slipped (1.8%) and ten vocalised (1.6%) although no incidences were directly associated with RP use. However, increased time restrained in the RP was significantly associated with more gate slams into the RP entrance gate. The use of the RP was not significantly associated with carcase bruising. These results are encouraging, and although it cannot be concluded that the presence of an RP improves cattle welfare at slaughter, use of automation within cattle slaughter facilities warrants further investigation.

Rodent control tends to involve methods that cause animal suffering, but little attention has been paid to the animal welfare implications of rodent control. The aim of the current study was to gain insight into the opinions and attitudes of clients of Dutch pest controllers, regarding liminal rodents, rodent control, and rodent welfare. A better understanding of their attitudes may contribute to more ethical rodent management programmes. An online survey among 248 clients of Dutch pest controllers was carried out. Respondents, especially those within the agricultural sector, have a relatively negative attitude towards rats and mice. Respondents in the agricultural subgroup do not consider the welfare of liminal rodents important. They also think that the welfare impact of commonly used control methods is limited, and they have low tolerance levels for the presence of rodents. Respondents from other sectors have a far more positive attitude towards rats and mice, consider their welfare to be of greater importance, have a greater estimation of the welfare impact of control methods and show greater tolerance levels towards rodents. The respondents from the latter subgroup have a similar attitude compared to Dutch pest controllers participating in a previous survey. The findings of the current study firstly provide useful information for the further development and practical implementation of preventive control methods. Secondly, they provide input for a more animal-friendly rodent control and for the development of an assessment framework to support ethical decision-making. Finally, they can be helpful for further research and the communication and co-operation between professional pest controllers and their clients.

The aim of this study was to assess welfare outcomes of electrical stunning as a means of restraint in farmed grower saltwater crocodiles (Crocodylus porosus). Physical handling of a stunned, unconscious crocodile is far safer for the operator than handling a fully conscious animal. Electroencephalogram (EEG) was recorded before and after the application of electrical stunning at 50 Hz or 400 Hz using an electrical stunner applied to the cranial plate (Position 1: P1–50 Hz; n = 31, P1–400 Hz; n = 29) or immediately behind the skull (Position 2: P2–50 Hz; n = 29; P2–400 Hz; n = 30). For all electrical stuns, percentage total EEG power in a 10-s epoch decreased in the alpha and beta frequency bands; and increased in the delta and lower frequencies bands. All electrical stuns resulted in increased strength of signal, based on the quadratic mean EEG power in all frequency bands of the EEG. Greatest change in power occurred in the delta frequency band, with P1–50 Hz. This was greater than with P2–50 Hz; while decibel change using 400 Hz at either position was intermediate and not significantly different from either. Application of either electrical stunner at position 1 resulted in seizure-like activity and activation in low frequencies, but at position 2 this was not consistent across all animals. The ability of the electrical stunning equipment to consistently induce recoverable unconsciousness could be ranked in decreasing order as: P1–50 Hz > P1–400 Hz = P2–50 Hz > P2–400 Hz. Based on behavioural observations, all animals in the study appeared to stunned however evaluation of duration of EEG changes indicates that use of the electrical stunning equipment at 50 Hz would allow some margin for inaccuracies in tong placement, while achieving a consistently reliable stun.

There has been limited research into the effectiveness of penetrating captive bolt (PCB) for stunning horses (Equus caballus) at slaughter. This study observed 100 horses at a commercial abattoir in Mexico, stunned using pneumatic PCB. Animals were assessed at the time of stunning and immediately after for signs of effective/ineffective stunning and shot positioning, with macroscopic gross brain pathology conducted to determine brain trauma. Twenty-five percent (25/100) received more than one shot and 28% (28/100) displayed behavioural signs of ineffective stunning. Of these 28 animals, all had deviations of more than 10 mm from the suggested shot position outlined by the Humane Slaughter Association with rostral-caudal deviation associated with an absence of damage to the thalamus, midbrain, and pons. Forty-four percent (44/100) of animals displayed no damage to critical brain structures (thalamus, midbrain, pons and medulla), with this associated with ineffective stunning. Overall, 16% of shots missed the brain (16/100), with a higher proportion of poll shots (30%) missing the brain compared to frontal shots (12%). There is the potential, when animals are shot into the poll, for paralysis from damage to the spinal cord and caudal brainstem structures. Appropriate position, angle and performance of PCB is therefore vital to achieving an effective stun, by targeting critical brain structures responsible for maintaining consciousness and ensuring proper PCB maintenance. Animals should be routinely checked between stunning and exsanguination, with minimal time between these stages, to minimise recovery of consciousness and alleviate suffering for horses at slaughter.

Large numbers of decapod crustacea are farmed and harvested globally for human consumption. Growing evidence for the capacity of these animals to feel pain, and therefore to suffer, has led to increased concern for their welfare, including at slaughter. In New Zealand, decapod crustacea are protected by animal welfare legislation. There is a requirement that all farmed or commercially caught animals of these species killed for commercial purposes are first rendered insensible. The aim of this study was to evaluate the efficacy of the Crustastun™, a commercially available bench-top electrical stunner, in two commercially important New Zealand crustacean species; the rock lobster (Jasus edwardsii) and kōura (freshwater crayfish [Paranephrops zealandicus]). Animals were anaesthetised via intramuscular injection of lidocaine and instrumented to record the electrical activity of the nervous system, prior to being stunned according to the manufacturer’s instructions. Stunning efficacy was determined by analysing neural activity and observing behaviour post stunning. All ten P. zealandicus and three J. edwardsii appeared to be killed outright by the stun. Of the remaining J. edwardsii, six exhibited some degree of muscle tone and/or slow unco-ordinated movements of the limbs or mouthparts after stunning, although there was no recovery of spontaneous or evoked movements. One J. edwardsii was unable to be stunned successfully, likely due to its very large size (1.76 kg). None of the successfully stunned animals showed any evidence of return of awareness in the five minutes following stunning. It was concluded that the Crustastun™ is an acceptable method for killing P. zealandicus and for stunning all but the largest J. edwardsii.

Helicopter-based shooting using either a .308 semi-automatic rifle or a semi-automatic 12-gauge shotgun is widely used to manage non-native ungulate populations in Australasia, but the animal welfare outcomes of these two firearms have not been robustly compared. We conducted a randomised field study to compare the animal welfare outcomes of helicopter-based shooting of fallow deer (Dama dama) using a shotgun with three types of lead-based shot (Winchester® 00 Buck, 1 Buck or 4 Buck) relative to a .308 rifle with 135-grain lead-based bullets in New South Wales, Australia, in 2023. All deer that were shot at (n = 390) were killed. Time-to-event curves for times from pursuit to first shot, first shot to insensibility, and the sum of these two metrics (‘total time’), were similar among the four ammunition types. The mean number of shots fired per deer was similar across all four ammunition types, but the mean number of wound tracts per deer increased across the four ammunition types with the number of projectiles per cartridge. All deer subjected to post mortem examination had $ \ge $ 1 wound tract or projectile in the thorax. Our study indicates that using a .308 semi-automatic rifle or a 12-gauge semi-automatic shotgun for helicopter-based shooting of non-native deer, when the latter is used at ranges ≤ 30 m, provides similar animal welfare outcomes.

There is limited research into horse slaughter, particularly ante mortem welfare, and the effectiveness of captive-bolt gun (CBG) stunning, despite this being a widely used method worldwide. To address this evidence gap and explore associations between ante and post mortem factors, the welfare of 62 horses was assessed at a commercial Italian abattoir. Animal-based measures were used to identify stress-related behaviours and stunning effectiveness. A sub-sample (44%; 27/62) of heads were assessed for gross brain pathology. All animals in the study showed stress-related behaviours at all stages of the slaughter process. Additionally, 53% (33/62) of horses slipped in the stunning box, with poor floor surface condition and use of force associated with this. At least one sign of an ineffective stun was observed in 22% (14/62) of animals. Six animals were shot twice, with the application of a second shot significantly associated with a higher stress behaviour index score in the stunning box. Damage to critical brainstem structures was found in 85% (23/27) of heads that were assessed with gross pathology. An absence of damage to critical brainstem structures meant that animals were ten times more likely to show signs of ineffective stunning. These results highlight the risks to equine welfare throughout the slaughter process and suggest that mitigating ante mortem stress could improve stunning effectiveness, whilst CBG usage should be refined to ensure that critical brainstem structures are targeted.

Over the last several decades an alternative to current methods of stunning cattle has been developed. This system, DTS: Diathermic Syncope®, has been suggested to the Jewish and Muslim communities as a means to achieve pre-cut stunning in conformity with both religious and EU regulations without a need to resort to a derogation that permits an exemption from the EU requirement to pre-stun all animals undergoing slaughter. The developer’s contention is that the system induces fainting, and thus should be acceptable to all groups, including the kosher (Jewish) and Halal (Muslim) consumer. A review of the system based on publications and reports from the developer itself suggests that in reality the system selectively heats the brain, leading to an epileptic-type seizure with tonic-clonic phases and unconsciousness lasting several minutes. It does not induce a (benign) faint, and use of the system might cause structural brain damage. Thus, this system is unlikely to be acceptable under Jewish religious law and its animal welfare value can be questioned.

Multiple benefits of freight rail activity have been shown for commercial agribusiness, yet the effects of freight rail-related noise and vibration on domestic livestock health and welfare has so far received little research attention. This scoping review examines peer-reviewed and grey literature addressing associations between freight rail noise, vibration and impacts on domestic livestock. Six databases (Scopus, Science Direct, SAGE, TRID, SPARK, ARRB) were searched for relevant literature published from 1980–2019. PRISMA search procedures were used to identify 28 publications relevant to domestic livestock, as well as noise or vibration impact of rail applicable to the freight rail context. Included publications addressed a range of livestock and related species, covering descriptive, review, and experimental findings on noise and vibration impacts. Five publications addressed vibration effects, and 23 addressed noise effects. Effects of noise and vibration on different species indicated that adverse effects vary depending on exposure intensity. The literature indicates that specific thresholds for noise and vibration exposure should be considered when managing freight rail impacts on commercial agribusiness involving avian and mammalian species. Freight rail noise and vibration likely exceeds thresholds for discomfort and harm for avian and mammalian species. Future research should consider case studies that specifically focus on integrating freight rail noise and vibration data to derive species-specific guides for animal health and welfare purposes.

The stranding of cetaceans raises significant welfare and associated logistical issues. A survey of opinions on euthanasia methods based on the recommendations of an International Whaling Commission (IWC) workshop was conducted. Descriptive statistics showed that the workshop recommendations were generally supported and understood, and these included the use of specific euthanasia techniques, but important barriers to adherence were identified, including lack of governmental support, lack of resources, and lack of experienced or trained personnel. Conjoint analysis of factors identified that ‘time to death’ was considered the most important determinant of welfare outcome. In view of the findings of this study, it is recommended that the IWC should consider creating a training programme for responders (both veterinary and non-veterinary) to ensure that trained personnel are available who can implement timely and tailored euthanasia techniques, if required, when strandings occur. Further research on this topic is also advocated to ensure a better understanding of what is being applied in different nations.

The aim of this study was to investigate the relative effectiveness of a rapidly expanding Bonded hunting bullet and an explosively expanding Varmint bullet in young harp seals (Pagophilus groenlandicus). The study was conducted as an open, controlled and randomised parallel-group designed field trial. The animals were pre-randomised (1:1) into one explosively expanding (Varmint) and one expanding (Bonded) bullet type group, with 75 animals in each. The study sample consisted of young, weaned harp seals, 2-7 weeks of age, of both sexes, from the Greenland Sea harp seal population. The study was conducted during the regular hunt. Instantaneous death rate (IDR) and time to death (TTD) were the main variables. The observed IDR was 84% in both bullet groups. Correcting for Weather Condition Index, the IDR for the Varmint bullet was significantly higher compared to the Bonded. The mean TTD was shortest in the Varmint group, but the difference did not reach significance. Compared to the Bonded, a significantly higher total cranial damage score and bleeding intensity, and significantly lower frequencies of bullet exit wounds were detected in the Varmint group. The post mortem reflex movements caused by the Varmint bullet were significantly more powerful with longer duration and higher frequencies of clonic contractions. In conclusion, the results indicate a higher effectiveness of the Varmint bullet relative to the Bonded. The Varmint bullet may thus improve animal welfare in the hunt of young harp seals.

Slaughter is considered an important fish welfare issue. For a slaughter method to be considered humane, effective stunning that lasts until death as well as the reduction of pain and fear throughout all procedures are essential. Our objective was to investigate current techniques for slaughtering fish in Brazil. A digital questionnaire with six multiple-choice and three open questions was distributed to companies registered as fish slaughterhouses and to fish farms in 2019, addressing fish species, quantities processed and slaughter procedures adopted. From 62 facilities in 15 Brazilian states that answered the survey, nine slaughterhouses and 30 fish farms slaughtered fish, totalling 452 tonnes per day. All reported pre-slaughter stunning, and live chilling to be the most commonly cited method (82.0%), followed by electronarcosis (18.0%). Slaughtering techniques included exsanguination (38.5%) and decapitation (2.5%). For the remaining companies, no slaughter method was declared, suggesting death by asphyxiation or by further processing (59.0%). Twenty-nine companies adopted pre-slaughter fasting, lasting from 10 to 48 h. All sites worked with tilapia (100%) and 24 (82.7%) reported that they worked exclusively with this species. Other species reported were: pacu (25.6%), tambacu (17.9%), tambaqui and carp (15.3%), jundiá (12.8%), pintado and pirarucu (7.7%), matrinxã and pangassius (5.1%). Asphyxia and live chilling are not considered humane, as animals remain conscious and, thus, suffer. Results show that most establishments do not perform humane slaughter. This scenario highlights the urgent need for development and enforcement of humane fish slaughter techniques, with routine supervision and normative requirements.

The ethical and economic significance of slaughtering animals for consumption by people of faith cannot be underestimated. On one hand, there are concerns for the welfare of animals during rearing, transport and slaughter, on the other, the market for halal meat products continues to grow at an exponential rate which has attracted the attention of independent and mainstream retailers. This paper considers the slaughter methods approved for the main animal species slaughtered for consumption by Muslims: beef, lamb, goats and poultry. It further examines the rationale for approving and rejecting certain methods of stunning and the implications this has for the welfare of animals. Areas where further research is needed to improve animal welfare during halal slaughter are also highlighted, and the authors have argued why a dialogue between animal welfare researchers, Islamic scholars and halal certification or accreditation bodies is vital in creating knowledge exchange between key stakeholders with a view to improving animal welfare during halal meat production.

This paper reviews the impact on fish welfare of a wide range of slaughter methods used commercially around the world. Because the end result of the slaughter is a food product, and because of the well-known relationship between an animal's welfare and subsequent meat quality, the effects of the slaughter methods on the quality of the flesh are also reviewed where possible. Fish slaughter methods are incredibly diverse, but fall into two broad categories: those that induce loss of sensibility slowly, and those that achieve this rapidly. This paper shows that, in general, the methods that induce loss of sensibility over a long period of time tend to impinge more on the welfare of the animal and are detrimental to the overall quality of the carcass. Methods that cause a rapid loss of sensibility result in the best welfare, providing that they are carried out correctly. They may also produce the highest quality product from the stock offish.