The intensification of pig (Sus scrofa domesticus) production systems raises concerns regarding animal welfare, particularly during pre-slaughter conditions, a phase associated with significant stress. Saliva is increasingly recognised as a non-invasive matrix for detecting stress-related biomarkers in pigs. This preliminary study aimed to explore salivary protein changes in pigs subjected to two distinct pre-slaughter conditions at the slaughterhouse, improved (Group A) and stressful (Group B), by tandem mass tag (TMT)-based proteomics. Proteomic analysis of saliva from three pigs per group revealed 13 proteins with a statistically significant difference in relative abundance between the groups. Group B showed elevated levels of proteins linked to metabolic stress, inflammation, and coagulation, such as cystatin-C and fibrinogen chains, while proteins like vimentin and follistatin-related protein were decreased. Cystatin-C and vimentin were further validated by immunoassays in 12 additional pigs per group, confirming their differential abundance. These findings suggest that salivary cystatin-C and vimentin, along with the other 11 proteins that showed changes at proteomics, may serve as candidate biomarkers of acute stress at slaughter. While further validation is required, our results support the potential of salivary proteomics for welfare monitoring in livestock.

Although there are public expectations regarding improvements to farm animal welfare, farmers’ well-being remains largely overlooked. This is particularly concerning given the high prevalence of physical and mental health issues among farming populations. As key stakeholders in the implementation of animal welfare practices, farmers play an essential role in welfare outcomes. Improving animal welfare may require addressing farmers’ own well-being. To support this hypothesis, it is necessary to examine the relationship between farmers’ well-being and the welfare of their animals. This scoping review aimed to: (1) map the methods used to describe relationships between farmer well-being and animal welfare in primary research; and (2) compile pieces of evidence of such relationships. Following the PRISMA extension for Scoping Reviews, the same search was carried out on three databases (Web of Science Core Collection, MEDLINE, CABI digital library). Twenty-two articles from the 10,189 retrieved met the inclusion criteria. Results underscored the need to standardise methods to enable cross-study comparisons, as different questionnaires were used to assess the same construct (e.g. four for psychological stress), and none of the animal welfare indicators were fully comparable. Moreover, 94 pieces of evidence regarding the relationships between farmer well-being and the welfare of their animals were compiled. Ninety-three pieces described positive associations where improved farmer well-being was associated with improved welfare of their animals, and vice versa. This result suggests that welfare improvement strategies on farms should address not only animal welfare, but also farmer well-being. The results therefore support a One Welfare approach on commercial farms.

In Australia, nearly twenty million cattle and sheep pass through saleyards annually, with potentially significant impacts on their welfare. This study documented the mortality rate occurring from January 2021 to December 2024 at a sample of saleyards of cattle and sheep in New South Wales, Australia, and identified possible risk factors. A database of the number of animals sold and deceased, either on arrival or while contained at each saleyard on each sale day, was created from the National Livestock Identification System. Descriptive statistics, and uni- and multivariable linear regression were used to examine risk factors for mortality. The mean sale mortality rates were 0.016 and 0.096% for cattle and sheep, respectively. In the univariate model, cattle sale mortality rate was associated with the maximum daily temperature, year, size of saleyard, and saleyard location, while minimum daily temperature, region, and saleyard location were associated with sale mortality of sheep. In the multivariable model, size of saleyard, saleyard location, month and year were significant predictors for the cattle mortality rate, while saleyard location and minimum daily temperature remained significant predictors of sheep mortality rate. Furthermore, sale mortality rate was eight times higher in sheep than in cattle, and sheep mortality was higher than values reported in the literature for farms. Further studies investigating the cause of death, journey conditions, and management practices of saleyards are recommended.

This study investigated the effect of group size on tail damage and growth performance in growing-finishing pigs with intact tails. A total of 432 pigs were housed indoors on fully-slatted floors and assigned to either small (nine pigs per pen) or large (18 pigs per pen) groups, with equal space and resource allocation per pig. No environmental enrichment was provided. From nine to 23 weeks of age, pigs were monitored weekly for tail injuries using a 5-point scale (0 = no injury, 4 = partial or total loss). The most severe score observed during each four-week period was used for analysis, and outbreaks were defined as the occurrence of one or more pigs per pen with a tail score ≥ 2. Group size did not influence average daily gain, feed intake, or feed to gain ratio. However, pigs housed in small groups experienced more frequent and severe tail injuries, including a higher proportion of removals due to tail wounds. In contrast, pigs in large groups were more likely to receive healed tail scores (score 1) or mild injuries (score 2), and experienced fewer removals. While these results suggest that tail damage may be less severe in larger groups, the total number of pigs affected by tail biting was similar across treatments. These findings highlight the importance of managing tail-damage severity and suggest that group size can influence welfare outcomes in systems where pigs are raised with intact tails.