This paper explores the complex role penicillin played in the relations between Britain, the USA and the USSR between the Second World War and the beginning of the Cold War through the lens of science diplomacy and the category of negotiation. In the post-war years the Soviets tried to acquire know-how on large-scale penicillin production from Britain and the USA. While the USA refused to collaborate, the British strategy was more complex. The British government allowed the Oxford team, which had discovered the antibacterial properties of penicillin, to disclose all the technology and know-how concerning large-scale penicillin production of which they were aware to the Soviets, while simultaneously trying to slow down penicillin research and production in the Soviet Union by controlling the export of certain industrial machinery, Podbielniak extractors, to Eastern Europe. By contrast, the USA put a stop to scientific and technological collaboration with the Soviets, but were less strict about the export of industrial machinery. The different strategies generated tensions between Britain and the USA, and ultimately mirrored both the British fear of an American disengagement from Europe and the American will to protect the interests of their national industry.