Human genetic structure of Iberian populations has been thoroughly explored in the last decades. The internal diversity of the Iberian Peninsula becomes visible by the different phylogeographic origins of particular mitochondrial DNA and Y chromosome lineages, which show a high degree of population specificity. In the present study, we combined information on matrilineal and patrilineal variation patterns in two autochthonous populations from Andalusia region (southern Spain). A special focus is made to a male sample set where both uniparental data are available. Gene diversities estimates yielded not statistically significant differences between both types of samples and markers. Genetic ancestry among Andalusians seems to be constituted by three foremost continental origins: European, African, and Middle Eastern. The examined male group has revealed a noticeable proportion of individuals (over 45%) with a non-correspondence between maternal and paternal haplogroup origins, a signal of different population demographic histories linked to both sexes in the past. Andalusian males seem to be well differentiated according to ancestries. As expected, mtDNA diversity was much higher than that for the Y chromosome, a fact that can be caused by patrilocality, which leads to particular social structures with effects on haploid genomes in modern human populations.

Cultural evolution of traditional music around the world has been the subject of recent quantitative investigations. Researchers have explored cultural diffusion of music as well as patterns of geographic variation that may result. By comparison, less has been studied about the process of music diversification; in particular, under what circumstances music diversifies is yet to be understood. In this study, we examine possible factors that may facilitate music diversification, using data from folk songs in the Ryukyu Archipelago, south-western islands of Japan. For a quantitative analysis, we first transform the melody of each folk song, following an automated scheme, into a sequence of alphabets, which is then used to quantify the melodic dissimilarity between each pair of songs. Our particular interest is in the dissimilarity between putative sister songs, or songs that are inferred to have derived from a common origin, and factors that have positive or negative effects on it. Our results suggest that sister songs tend to diversify more when they are sung in different islands, probably as a result of one being transmitted from one island to another, and when they have come to be sung in different social contexts.

Lexical borrowing may provide valuable clues about the sociohistorical context of language contact. Here we explore patterns of vocabulary transfer between languages from three families (Kx’a, Tuu, Khoe-Kwadi) comprising the linguistic unit commonly referred to as Southern African Khoisan. In our data set, 20% of 1,706 roots are shared between at least two families. By applying a carefully chosen set of linguistic and extralinguistic criteria, we were able to trace the origin of 71% of shared roots, with the remaining 29% constituting good candidates for ancient contact or shared common ancestry of the forager families Kx’a and Tuu. More than half of the shared roots for which an origin could be determined trace back to Khoe-Kwadi and were borrowed into languages of other families within two major confluence zones with different sociohistorical profiles: (i) the Central Kalahari characterized by egalitarian interaction between languages of all three families and (ii) the southern and south-western Kalahari Basin fringes showing unilateral transfer from Khoe-Kwadi-speaking herders into resident forager groups. The findings of this study complement genetic and archaeological research on southern Africa and testify to the value of linguistics in the multidisciplinary inference of contact and migration scenarios.

The blowgun is a weapon that employs the force of breath for expelling a projectile and has been traditionally used for hunting and (occasionally) war. The use of blowguns extends to ancient times and is advantageous in dense-forest areas of South America and South East Asia. A classification system of blowgun types introduced in 1948 for South America is extended here. We assembled a global database that includes collection data and ethnographic accounts of blowgun types and other related features that were linked to available linguistic information. Our analyses show that geography explains the distribution of blowgun types to some degree, but within regions of the world it is possible to identify cultural connections. Darts are by far the most used projectiles and in combination with toxins (e.g. curare), these weapons reach their highest potential. A case study on the use of blowguns in groups of Austronesian language speakers shows clade-specific preferences across the tree. Our comprehensive database provides a general overview of large-scale patterns and suggests that incorporation of other related data (e.g. sights, mouthpieces, quivers) would enhance the understanding of fine-scale cultural patterns.

In mammals, pregnancy and lactation are marked by maternal calcium stress and bone resorption, leading to reduced bone mineral density. In humans, these periods may partly explain the higher prevalence of osteoporosis in older women compared with men, but lactation patterns in modern humans may reflect cultural influences rather than natural conditions. The extent to which these findings apply to wild-living mammals remains unknown. We measured urinary C-terminal crosslinking telopeptide of Type I collagen (CTX-I) levels, a bone resorption marker, during pregnancy in wild and zoo-housed bonobos (Pan paniscus) and during lactation in wild bonobos. Studying wild-living primates such as bonobos can provide insights into ancestral reproductive adaptations. We found an increase in CTX-I levels towards the end of pregnancy in zoo-housed and primiparous wild females. Contrary to expectations, CTX-I levels during early lactation are lower than in other reproductive phases. This pattern diverges from the assumption that lactation increases bone resorption. Our findings suggest that wild bonobos may rely on a combination of physiological and behavioral strategies to modulate bone metabolism during lactation. Bone resorption may serve as a physiological back-up when behavioral or dietary strategies cannot fully meet calcium demands. These flexible responses, shaped by fluctuating environmental conditions and prolonged maternal investment, provide insight into evolutionary pressures on skeletal health and may inform strategies to mitigate bone loss in humans.

How does information infrastructure shape long-term cultural evolution? Using over four centuries of professional game records from the game of Go, this study explores how strategic dynamics in opening moves reflect historical shifts in the ‘infostructure’ of skilled Go players. Drawing from recent work on how population size, AI, and information technology affect cultural evolution and innovation dynamics, I analyze over 118,000 games using measures of cultural diversity, divergence, and player network composition. The results show distinct eras of collective innovation and homogenization, including an early 20th-century explosion of novel opening strategies, a Cold-War-era die-off, and a recent increase in evolutionary tempo with the arrival of the internet and superhuman AI programmes like AlphaGo. Player population size shows an inverse-U relationship with opening move diversity, and a recent decline in strategic diversity has accompanied a shift in the player network, from many small subgroups to a few large ones. Surprisingly, the influence of AI has produced only a modest, short-lived disruption in the distribution of opening moves, suggesting convergence between humans and AI and incremental rather than revolutionary cultural change.

Humans often participate in physically harmful and demanding rituals with no apparent material benefits. Although such behaviours have traditionally been explained using the lens of costly signalling theory, we question whether the canonical theory can be applied to the case of human cooperative signals and introduce a modification of this theory based on differential benefit estimation. We propose that along with cooperative benefits, committed members also believe in supernaturally induced benefits, which motivate participation in extreme rituals and stabilize their effects on cooperative assortment. Using Thaipusam Kavadi as a prototypical costly ritual, Tamil (ingroup) and Christian (outgroup) participants in Mauritius (N = 369) assessed the cost and benefits of Kavadi participation or hiking. We found that ingroup participants estimated material costs as larger than outgroups, physical costs as lower, and benefits as larger. These findings suggest that estimated costs may vary by modality and cultural expectations (e.g. Kavadi participants are not supposed to display pain), while supernaturally induced benefits were consistently reported as larger by ingroups compared to outgroups. We conclude that differential estimation of ritual benefits, not costs, are key to the persistence of extreme rituals and their function in the assortment of committed members, underscoring the role of differential estimation in the cognitive computation of signal utility.

Arcade video games evolved in a constrained design space, following patterns of diversification, stabilisation, and collapse that mirror macroevolutionary processes. Despite their historical significance and detailed digital records, arcade games remain underexplored in cultural evolution research. Drawing on a dataset of 7,205 machines spanning four decades, we reconstruct the evolutionary trajectories of arcade niches using a multi-scale framework that integrates trait-level innovation, genre-level selection, and systemic constraints. We identify two contrasting dynamics: (1) resilient genres—such as Fighter and Driving—maintained long-term viability through innovation and collaboration networks, while (2) early Maze and Shooter subgenres collapsed due to imitation and weak collaboration. Morphospace analysis reveals how technological traits—specifically CPU speed and ROM size—co-evolved with gameplay complexity, shaping the viable design space. We argue that genres operated as evolving cultural-ecological units—structured niches that shaped trait evolution through reinforcement, constraint, and feedback. This multi-scale perspective positions arcade games as a rich model system for studying cultural macroevolution.

In Africa, harps exhibit significant morphological diversity, yet their historical trajectory remains largely underexplored. Phylogenetic reconstruction methods offer valuable tools for understanding this diversity and the relationships between groups of harps. This study is among the first to apply one of these methods, cladistics, to the morphology of a musical instrument, analysing 318 harps and 83 characters. We present a well-resolved phylogenetic tree, which shows several clades corresponding to geocultural regions, in alignment with ethnomusicological classifications. We show that this tree robustly represents the patterns of vertical transmission in the cultural evolution of harp morphology across Africa, despite the limited contribution of several tested characters. Additionally, a comparison with previous research reveals that characters coding decorations exert a minimal influence on the vertical evolution of these musical instruments. These findings provide valuable insights into the cultural evolution of harps on a continental scale, offering a clearer understanding of their diversity and revealing major evolutionary mechanisms.

Culture consists of practices – behaviour patterns – shared by members of a group. Some attempts to demonstrate evolution of cultural practices in the laboratory have shown evolution of material products, such as paper aeroplanes. Some attempts have shown evolution of actual group behaviour. The present experiments demonstrated evolution of group coordination across generations in punishing defection in a public-goods game. Cost of punishing defection varied across replicates that consisted of series of groups (generations) of 10 undergraduates each. Each generation played the game anonymously for 10 rounds and could write messages to the other participants and punish defection every round. The effectiveness of punishment depended on the number of participants choosing to punish. In Experiment 1, cultural transmission from generation to generation consisted of written advice from one generation read aloud to the next generation. In Experiment 2, transmission from generation to generation consisted of having some participants return from the previous group. The cost of punishing varied across replicates: zero, one, two or five cents. In both experiments, the evolution of altruistic punishing was strongly dependent on the cost of punishing. The results add to plausibility of studying evolution of complex behaviour patterns like cooperation in the laboratory.

This register-based study investigates how natural selection acts on educational attainment and reproductive timing among Estonians born between 1925 and 1977. Women with primary education consistently achieved the highest reproductive success throughout the study period, whereas a positive educational gradient in reproduction emerged among men born since the 1950s, resulting in sexually antagonistic selection on educational attainment. Men with tertiary education had higher reproductive success than other men, despite initiating reproduction later. The lowest-educated women exhibited the strongest selection for early reproduction and the earliest start of reproduction throughout the study period. These women and the least-educated men also experienced the strongest selection for delayed reproductive cessation. Nonetheless, parents with primary education (particularly men) were typically the first to stop reproducing. Stabilizing selection for intermediate interbirth intervals also showed the strongest quadratic selection gradients among minimally educated parents of both sexes. At that, men with primary education had the fastest reproductive pace, whereas women in the same group had the slowest. Our study shows that selection on reproductive timing traits was consistently stronger among parents with lower educational attainment, and that variation in reproductive timing across educational strata does not consistently reflect the selective pressures acting on recent generations.

We investigate and compare the evolution of two aspects of culture, languages and weaving technologies, amongst the Kra-Dai (Tai-Kadai) peoples of southwest China and Southeast Asia, using Bayesian Markov-Chain Monte Carlo methods to uncover phylogenies. The results show that languages and looms evolved in related but different ways and bring some new insights into the spread of the Kra-Dai speakers across Southeast Asia. We found that the languages and looms used by Hlai speakers of Hainan are outgroups in both linguistic and loom phylogenies and that the looms used by speakers of closely related languages tend to belong to similar types. However, we also found differences at a deep level both in the details of the evolution of looms and languages and in their overall patterns of change, and we discuss possible reasons for this.

Bipedalism is a distinguishing feature of our species and, as such, there has been much interest in the energetic costs and foraging returns of walking and running, especially among hunter-gatherer societies. However, humans routinely exhibit extensive locomotor versatility, with hunter-gatherers consistently also swimming, diving, and climbing. Additionally, the fitness costs and benefits of locomotion extend well beyond energy income and expenditure. Here, we review evidence from over 900 ethnographic documents across a worldwide sample of more than 50 hunter-gatherer societies to examine the fitness costs and benefits of walking, running, climbing, swimming, and diving. We show that the fitness costs and benefits of locomotor engagement consistently extend well beyond energetics to include, for example, currencies of status, protection from hazards, and risks of injury or death. These fitness factors differ in significance between locomotor modalities, with implications for the comparison of bipedal and non-bipedal locomotion. For example, while energetic demands represent the major cost of most bipedal engagements, the fitness implications of potential fall injuries may outweigh those of energetics in tree climbing. These results inform existing debates relating to hominin locomotor evolution and hunter-gatherer behavioural ecology.

Sex-specific division of labour and the associated use of different subsistence techniques by males (e.g. hunting) and females (e.g. gathering) has played an important role in shaping human societies. Skills needed in adulthood are practiced in play during childhood and object play has been proposed to foster tool-use skills necessary for adult subsistence techniques. Here, we investigated sex differences in the ontogeny of object play in Tsimane children in Bolivia to understand its potential role in shaping gender-specific adult roles. We used observational data (>80,000 scan samples) from nine Tsimane communities collected between 2002 and 2007. We analysed age and sex differences in general play, object play, and object types. Our results show that both general play and object play peaked in early to middle childhood (3.5–7.5 years of age), with boys spending more time playing. Moreover, boys engaged more with objects related to male-specific roles (e.g. hunting tools), while girls played more with objects related to female-specific roles (e.g. cooking tools). Our findings suggest that object play serves as an adaptive, culturally embedded pathway to develop gender-specific adult skills. Studying developmental patterns of object play across human cultures enriches our understanding of the evolutionary contexts shaping divisions of labour.

Despite growing consensus that cognitive differences between Neanderthals and modern humans were not as significant as once assumed, visual art remains disproportionately associated with Homo sapiens. This paper explores why that is. Rather than appealing to underlying cognition, we argue that early art was pushed and pulled by social dynamics and demographic contexts on a larger scale and eventually formed through cultural transmission. Drawing on the archaeological record, we focus on pigment use, ornamentation, engravings and painting. We suggest that these practices initially remained ad hoc while still functioning as tools for social signalling and intergroup communication in the small-scale communities of Neanderthals and early modern humans. We argue that visual art moved beyond ad hoc practices under conditions of increased population density and interaction – first via the crystallization of art as a tradition and second, as cumulative cultural art. Lack of cognitive ability is not a well-supported explanation for the empirical low frequency of Neanderthal art. Instead, we propose effects of differences in social connectivity and population densities as an alternative. By reframing art as a flexible, context-dependent behaviour, we challenge essentialist models and advocate for a pluralistic view of cognitive and cultural expression across different hominin groups.

Recent years have seen growing interest in applying the Evolutionary Transitions in Individuality (ETI) framework to human sociocultural evolution. Proponents argue that human societies exhibit features – such as multilevel organization, cooperation, and division of labour – sufficiently analogous to biological ETIs to warrant theoretical extension. This paper critically assesses such claims and argues that they rest on a fundamental misapplication of the ETI framework. Drawing on recent work in cultural evolution, I show that sociocultural systems typically lack the core conditions required for an ETI, including autonomous reproduction at the group level and the operation of natural selection in the reproductive mode. Attempts to relax these criteria risk undermining the coherence of the framework itself. I conclude that although the broader framework of Major Evolutionary Transitions may still have value for understanding sociocultural change, the specific explanatory structure of ETI theory does not transfer.

The manosphere is a collection of online antifeminist men’s groups whose ideologies often invoke Darwinian principles and evolutionary psychological research. In the present study, we reveal that the manosphere generates its own untested and speculative evolutionary hypotheses, or ‘just-so stories’, about men, women, and society. This is a unique phenomenon, where lay (i.e. non-expert) individuals creatively employ evolutionary reasoning to explain phenomena in accordance with their particular worldview. We thus assembled the first dataset of lay evolutionary just-so stories extracted from manosphere content (n = 102). Through qualitative analysis, we highlight the particularity of the manosphere’s lay evolutionism. It is a collective bottom-up endeavour, which often leads to practical advice and exhibits a male gender bias. We further show that 83.3% of manosphere just-so stories pertain to sex differences and that only 36.3% explicitly signal that they are speculative. Given this evidence that lay communities collectively engage in evolutionary hypothesizing, we reflect on implications for evolutionary scholars and for the field as a whole in terms of ethics and public image. Lastly, we issue a call for renewed discussion and reflection on evolutionary hypothesizing, a central yet somewhat neglected feature of evolutionary behavioural science.