Greenhouse and laboratory experiments were conducted to determine if the synergistic interaction between imazethapyr and thifensulfuron in sulfonylurea-tolerant (STS) soybean involved enhanced absorption and translocation or reduced metabolism of one or both herbicides. Thifensulfuron at 4.4 g ha−1 and imazethapyr at 70 g ha−1 caused 0 and 28% injury to STS soybean 7 DAT, respectively, while the combination of both herbicides caused 50% injury 7 DAT. Imazethapyr had no effect on absorption of 14C-thifensulfuron into the first trifoliolate. Imazethapyr did not affect absorption, translocation, or metabolism of 14C-thifensulfuron. Metabolism of 14C-thifensulfuron was rapid, with less than 10% remaining after 24 h, and was not affected by imazethapyr. Likewise, thifensulfuron did not affect the absorption, translocation, or metabolism of 14C-imazethapyr. Therefore, the synergism between thifensulfuron and imazethapyr does not involve changes in the absorption, translocation, or metabolism of either herbicide.

The efficacy of the safeners naphthalic anhydride (NA), R-29148, benoxacor, and dichlormid as protectants of corn against injury from the methyl ester of tribenuron was evaluated under greenhouse conditions. Tribenuron applied alone reduced the root length of both ‘Pioneer 3180’ and ‘Northrup-King 9283’ corn. The concentration of tribenuron causing 50% reduction of corn root length (GR50) was 1 μM. NA applied as a seed dressing at 0.5 and 1.0% (w/w) was the most effective of the four safeners tested and provided excellent protection to both corn hybrids against 1, 2, and 4 μM tribenuron. Soaking corn seeds in an aqueous solution of R-29148 containing 0.5,1, or 2 mM protected ‘Pioneer 3180’ against 1 μM of tribenuron. The safening efficacy of R-29148 against tribenuron injury to ‘Northrup-King 9283’ corn was limited. Dichlormid and benoxacor, were less effective as tribenuron safeners and offered partial protection to both corn hybrids. Individual applications of the synergist piperonyl butoxide (PBO) at 0.1 and 0.2 mM did not affect the growth of corn seedlings. PBO synergized the activity of all concentrations of tribenuron, but only on the ‘Northrup-King 9283’ hybrid. These results demonstrate that the tolerance of corn to tribenuron can be enhanced with the use of safeners such as NA and R-29148, and that oxidative reactions may be involved in the metabolism of tribenuron by corn.

Research was conducted to elucidate mechanism(s) of nicosulfuron and primisulfuron selectivity in corn, woolly cupgrass, and shattercane. Corn absorbed less than one half the nicosulfuron and primisulfuron that woolly cupgrass and shattercane absorbed. Acetolactate synthase (ALS) isolated from three species responded similarly to nicosulfuron and primisulfuron. Values for 50% (I50) inhibition of ALS for nicosulfuron were 36.9, 37.2 and 29.5 nM for corn, woolly cupgrass and shattercane, respectively. Primisulfuron I50 values were 13.8, 11.5 and 10.0 nM for corn, woolly cupgrass and shattercane, respectively. Shattercane's I50 was different from corn's but not from that of woolly cupgrass. Corn rapidly metabolized nicosulfuron and primisulfuron, with a half-life of less than 4 h. Shattercane metabolized the herbicides more slowly, with a half-life greater than 72 h for nicosulfuron and 36 h for primisulfuron. Nicosulfuron and primisulfuron half-lives were greater than 72 h and less than 4 h, respectively, in woolly cupgrass. Therefore, selectivity with nicosulfuron and primisulfuron is likely based on metabolism to nonphytotoxic compounds. Corn tolerance to nicosulfuron and primisulfuron was also attributed to reduced herbicide penetration and translocation below the treated leaf.

N fertilizers suppress witchweed plant growth and development, thus reducing the severity of parasite attack and increasing host yield simultaneously. However, the underlying physiological mode of N action occurring within the parasite cells remains largely unknown. This study aims at screening for the effects of N forms and different growth conditions on some N assimilation enzymes in witchweed seedlings grown aseptically without host plant, and in pots with host plants. Results show that supply of N in NH4+ or urea forms resulted in 83 to 92% reduction in nitrate reductase activity (NRc), compared with control. Increasing NO3− concentrations from 0 mM to 100 mM, led to a corresponding increase in NRc in giant witchweed. NRc of giant witchweed seedlings grown under light and dark cycles were about 270 times higher than seedlings grown in continuous darkness. A combination of NH4+ and NO3−, resulted in increased giant witchweed NRc, compared with NH4+ or NO3− supplied singly. Highest shoot development and NRc was at NH4+ and NO3− ratio 1:1, followed by ratios 1:3, 3:1, 0:1, and 1:0, respectively. Addition of N in soils resulted in increased NRc, followed by rapid deterioration and death of giant witchweed plants. NRc, GSc, and GDHc in witchweed, maize, cowpea, and tobacco were affected by diurnal fluctuations with higher enzyme activities occurring during the day than at night. Higher GSc than GDHc suggests that NH4+ assimilation occurs mainly through the GS pathway in witchweed plants. NRc and GDHc were two and four times higher in giant witchweed grown in aseptic media without host plant, than that grown in potted soils with host plants. These findings provide insight into the physiological mode of N action and their implications on witchweed control.

White light-grown seedlings of smallseed dodder were (a) provided with unilateral far-red (700 to 800 nm) at photon irradiances ranging from 20 to 110 μmol m−2 s−1 against a background of cool white light (400 to 700 nm) from above at 77 μmol m−2 s−1, or (b) transferred to darkness and provided with unilateral white light at 20 μmol m−2 s−1, unilateral blue light (400 to 500 nm) at 10 μmol m−2 s−1, unilateral red light (600 to 700 nm) at 10 μmol m−2 s−1, unilateral far-red at 50 μmol m−2 s−1, or (c) in experiments utilizing bilateral irradiations, provided with unilateral far-red perpendicular to unilateral white light. Positive phototropic curvature was induced by unilateral white light and by unilateral blue light in otherwise darkness and by unilateral far-red in a background of cool white light. Seedling vines were also phototropic toward unilateral far-red when provided with unilateral white light perpendicular to unilateral far-red. Phototropism to unilateral white light was inhibited in seedlings treated with 200 μM norflurazon and 50 mM potassium iodide. Norflurazon- and potassium iodide-treated seedlings remained phototropic toward unilateral far-red when provided with unilateral white light perpendicular to unilateral far-red. Seedling vines were not phototropic to unilateral red or to unilateral far-red in otherwise darkness, and seedlings in cool white light were neither skototropic (i.e., tropic toward unilateral darkness) nor tropic to or from infra-red (radiation with wavelengths greater than 900 nm). Phototropism toward regions of lowered red:far-red may aid smallseed dodder in chlorophyllous host location and attachment.

Growth chamber experiments evaluated the influence of ambient temperature and soil moisture on cotton and velvetleaf response to pyrithiobac. Additional studies determined the basis for observed plant responses to 14C pyrithiobac. Cotton injury from six times the normal dosage was < 20% at 2 wk for all temperatures and soil moistures. Pyrithiobac injured velvetleaf less at lower soil moistures. Both species absorbed more 14C-pyrithiobac at 30/28 or 35/33 C than at 25/23 C. Cotton absorbed more herbicide than velvetleaf at all temperatures and soil moistures. Velvetleaf translocated < 16% of absorbed 14C out of the treated leaf while cotton translocated < 3% of absorbed material. At warmer temperatures, velvetleaf translocated less 14C when soil was dry (–1.0 MPa) than when plants were watered to field capacity (–0.03 MPa). This decreased absorption and translocation may affect pyrithiobac activity on velvetleaf growing in dry soil. Translocation differences did not fully explain whole plant effects. The metabolism difference may account for cotton tolerance.

A study was conducted to evaluate the response of common and giant ragweed to postemergence applications of imazethapyr using relative growth rate parameters. The relative growth rate was the same for untreated common and giant ragweed through the 21 d harvest interval. Relative growth rates of treated common and giant ragweed were 50% lower than the relative growth rates of untreated ragweeds 21 d after treatment. Between 21 and 56 d after treatment, the relative growth rate of common ragweed declined an additional 13%, while the relative growth rate of giant ragweed declined an additional 38%. The sharp continued decline in the relative growth rate of giant ragweed indicated plant death. The moderation and slight increase in the relative growth rate of common ragweed between 21 and 56 d corresponded with the initiation of lateral axillary buds and the regeneration of plant growth. Relative growth rate parameters identified differences in the response of common and giant ragweed to imazethapyr as early as 21 d after treatment. Relative growth rate demonstrated utility by objectively measuring differences in the growth response of these two weeds that are moderately susceptible to imazethapyr under laboratory conditions.

Rate of seedling emergence, relative growth rate (RGR), and phenological development were compared in several accessions of rigid ryegrass belonging to three distinct resistance classes. The aryloxyphenoxypropionate resistant (AOPP-R) class had a faster and less variable seedling emergence than the sulfonylurea resistant (SU-R) and susceptible (S) classes. However, even the fastest of the AOPP-R accessions was within the range of the S and SU-R classes. No significant differences were detected among the resistant classes in seed dormancy, RGR, and the rate of phenological development. The rate of spike emergence, irrespective of the resistance class, was related to the latitude of the origin of the accessions, suggesting adaptation to the local climates since introduction. Due to considerable variation among weed populations for most biological attributes, the need to include several R and S accessions, in studies similar to the one reported here, is of vital importance. Because of the means and variances of the three resistance classes, at least four accessions from each resistance class would have been required to detect the observed differences between emergence rates of the AOPP-R and S classes with a confidence of 95%.

Research was conducted to formulate a temperature-dependent population level model for johnsongrass seed germination and rhizome bud break. A nonlinear poikilotherm rate equation was used to describe development rate as a function of temperature, and a temperature-independent Weibull function was used to distribute development times for the population. Seed germination and initiation of rhizome bud break of johnsongrass were collected under constant temperature conditions to parameterize the model. Seed germination rate increased with temperature up to 36 C, then declined at 40 C. Rate of rhizome bud break increased with temperature up to 32 C, then rapidly decreased with further temperature increases. Rate of rhizome bud break was higher than for seed germination at temperatures of 32 C or below, but lower at higher temperatures. Time to first germination or bud break event was longer for seed than for rhizomes, but subsequent progression of development was higher for seed. A population level temperature-dependent model was developed by coupling the poikilotherm equation with the Weibull function. The model was validated against two independent seed germination and three independent rhizome bud germination data sets.

Research was conducted to formulate a temperature-dependent population-level model for rhizome johnsongrass flowering. A nonlinear poikilotherm rate equation was used to describe development as a function of temperature and a temperature-independent Weibull function was used to distribute development times for the population. Johnsongrass flowering data were collected under constant temperature conditions to parameterize the poikilotherm rate equation and Weibull function. Coupling the poikilotherm rate equation with the Weibull function resulted in a population level temperature-dependent model. The model was validated against independent field data sets. The model accurately predicted rhizome johnsongrass flowering from plants emerging in the spring. The model performed poorly for plants emerging in summer. Adjustments to the high-temperature inhibition parameter of the poikilotherm rate equation improved model performance in the summer without affecting spring predictions.

An estimation of the critical period of weed control is helpful in formulating appropriate weed-control strategies. A regression approach is presented to estimate the thresholds of critical period of weed control and time of equal interference (or time of onset of competition). In this approach, yields were either a linear or logistic function of the duration of weed-free and weed-infested periods. Confidence intervals of the thresholds of critical period and time of equal interference were determined for the linear model. An approximation to the standard error of critical period and associated confidence interval were given for any general form of the model. The method was applied to estimate the critical period of weed control in rainfed lentil using data from four field experiments conducted in Jordan. The relationship of yield with the duration of weed-free period was described by a linear function, whereas the relationship with the duration of weed-infested period showed a better fit with a logistic function. To maintain 90% of maximum seed yield, the maximum time allowed to let weeds grow after the crop emergence varied over locations from 4.8 to 5.8 wk. The same level could be achieved if the crop is kept free of weeds from its emergence until 12.1 to 14.1 wk; while the time when the same amount of yield would be achieved under both approaches varied from 7.7 to 9.3 wk after crop emergence. For straw yield, the time to get 90% of the maximum yield could vary over location from a maximum of 4.5 to 8.0 wk under weed-infestation and from at least 11.5 to 13.5 wk when weed-free. The time to achieve the same amount of straw under two systems of competition varied from 6.5 to 9.9 wk after crop emergence. One of the four experiments showed a longer critical period than the others for seed and straw yields.

The effects of acid scarification, prechilling, summer irrigation, and root extract stimulant from faba bean plants grown during different seasons on crenate broomrape seed dormancy and germination were determined. Immersion of 6- to 12-mo old seed in 0.5 to 5 N H2SO4 solution from 1 to 20 min increased germination by 20%. Similarly, seed pretreatment at temperatures between −4 and 6 C increased germination, regardless of the pretreatment duration. Results suggest that the hard seed coat is an important dormancy mechanism in newly formed crenate broomrape seeds. Summer irrigation did not substantially change the germination capacity of crenate broomrape seeds. The germination stimulant activity of the root extract from faba bean plants grown during different seasons varied considerably, decreasing in this order: autumn > winter > spring > summer. This suggests an ecological adaptation of crenate broomrape to its main host crop.

Sixteen experiments have investigated alternative methods of predicting the competitive effects of a simulated weed (oats) on the yields of spring barley, spring oilseed rape (canola), peas, spring field (faba) beans and flax. The experiments were designed to discover whether early postemergence assessments of crop and weed vigor would achieve more reliable prediction of yield loss than weed density. Weed density (plants m−2) was a very variable predictor of yield loss. The standardized ranges (range/mean) of values over 3 to 4 years of data for the five crops, in the densities causing 5% yield loss, were between 1.14 and 2.59. Predictions based on the relative dry weight of crop and oats (oat dwt/(oat dwt + crop dwt)), assessed while the plants were still small, achieved more reliable predictions, as the standardized ranges were between 0.10 and 1.86. In three of the experiments, predictions based on relative dry weights were compared to similarly timed predictions based on measurements of relative leaf area and of ground cover, assessed subjectively (by eye) and photographically. Subjective and objective (photographic) assessments of cover achieved similar predictions of yield loss, indicating that visual assessments could be a viable tool to assess the potential competitive effects of weeds.

Predictions of weed seedling populations from seedbank data should characterize the spatial distribution as well as the composition and abundance of weeds. The spatial distribution of seedbank and seedling populations of common lambsquarters and annual grasses (giant foxtail, large crabgrass, and fall panicum) were described in moldboard plow and no-tillage soybean fields from 1990 to 1993. Spearman rank correlations between seedbank and seedling densities were significant for common lambsquarters in both tillages and all years, but for annual grasses correlations were significant only in no-tillage. Semivariograms showed spatial autocorrelation in seedbank and seedling populations of common lambsquarters in all years in no-till, but less often in the moldboard plow field. Annual grass seed and seedling populations were autocorrelated in the no-till field every year except 1993, and in the moldboard plow field in 1992 and 1993 only. Cross-semivariograms showed spatial continuity between seedbank and seedling population densities in 3 of 4 yr in no-till for common lambsquarters, and in all years of no-till and 1 yr of moldboard plow for annual grasses. Grey-scale field maps of common lambsquarters seedbanks corresponded visually to maps of seedling populations and could have been used to target control efforts, but visual correspondence between annual grass seedbank and seedling maps was poor. Seedbank and seedling mapping may be useful for site-specific management, but additional information is needed to understand the variation in the relationships between these two populations over time and space.

The crop-weed interference relationship is a critical component of bioeconomic weed management models. Multi-year field experiments were conducted at five locations to determine the stability of corn-velvetleaf interference relationships across years and locations. Two coefficients (I and A) of a hyperbolic equation were estimated for each data set using nonlinear regression procedures. The I and A coefficients represent percent corn yield loss as velvetleaf density approaches zero, and maximum percent corn yield loss, respectively. The coefficient I was stable across years at two locations, but varied across years at one location. The coefficient A did not vary across years within locations. Both coefficients, however, varied among locations. Results do not support the use of common coefficient estimates for all locations within a region.

Weed seed return and seedbank composition, with particular reference to common lambsquarters, were studied in four tillage systems established on a site near Fingal, Ontario. The tillage treatments were moldboard plow, chisel plow, ridge-till, and no-till. The cropping system was a cornsoybean rotation. Tillage effects on weed population composition were assessed after all weed control measures had been implemented. More than 60% of the weed seedbank was concentrated in the upper 5 cm of soil in chisel plow and no-till. The seedbank of the moldboard plow system was more uniformly distributed over depth and larger than the other systems. Common lambsquarters comprised more than 50% of the seedbank in all systems except ridge-till, but only dominated the aboveground weed population in chisel plow. Seedbank populations of common lambsquarters with moldboard plowing were greater than those with ridge-till and no-till, and chisel plow seedbank populations were greater than those in ridge-till. Chisel and moldboard plow systems generally had higher aboveground plant populations of common lambsquarters than the other two systems. Seed production per plant by common lambsquarters was equivalent among the four systems, but estimated seed production per unit area was higher in moldboard plow and chisel plow systems than in the other systems. Populations of common lambsquarters and similar species may produce more seeds and persist in moldboard plow and chisel plow systems; these weeds may produce fewer seeds per unit area and be easier to manage in no-till and ridge-till systems.

Photographic techniques and the point-frame transect method were used to study areal growth of kikuyugrass grown in the field without competition in 1990 and 1991. The effect of cutting height on plant extension was also examined in 1991. There were no differences in sward area between eight selections planted in 1990, except on the first measurement date, but selections differed in height and number of primary and secondary stolons. Expansion was irregular in all but one selection, and the patch shape of all irregular selections was defined by the direction and number of the earliest buds to sprout from the stolons. Only two of the eight selections were planted in 1991. Cutting height affected extension from the plot center, linear plant frequency, and plant biomass. The lowest height of cut, 2.5 cm, had the least extension and frequency, the higher cut, 5 cm, was intermediate, and the unmowed plots had the highest extension and frequency. There were differences between selections in number of nodes per unit area, which resulted in differences in response to mowing. Results from a greenhouse clipping experiment showed that although root weight of stolon sections used as propagules was reduced by defoliation, starch content was not affected. The spreading ability of kikuyugrass appears to be based on its ability to branch from its stolons presumably due to reallocation of resources. Based on these results, close mowing could slow the process of kikuyugrass invasion into other turf species but is inadequate to control it.

Competitive relationships between johnsongrass, smooth pigweed, and soybean were examined in 1986 and 1987, and the adequacy of an additive response model (ARM) and product response model (PRM) in predicting yield reductions due to multispecies weed populations was assessed. A severe drought reduced soybean yields statewide in 1986, whereas ideal conditions for soybean production were experienced in 1987. Averaged over monospecific weed densities of 1, 2, 4, and 8 plants 4.6 m−1 of row, smooth pigweed intercepted 2.5 and 1.8 times more light than johnsongrass in 1986 and 1987, respectively. In multispecies populations having either 4 or 8 smooth pigweed plants 4.6 m−1 of row, light interception by johnsongrass was negligible. Averaged over monospecific weed densities, smooth pigweed produced 5160 and 1760 kg ha−1, while johnsongrass produced 1530 and 450 kg ha−1 dry weight in 1986 and 1987, respectively. In multispecies populations, smooth pigweed contributed more than 80% of the total weed biomass. As monospecific johnsongrass density increased, reductions in soybean seed yield were linear, whereas exponential response patterns adequately characterized reductions in soybean seed yield due to smooth pigweed interference. Based on calibrated monospecific responses, the ARM and PRM generally projected higher soybean seed yield reductions for multispecies weed populations than were observed. When crop production conditions were favorable and competitive effects of weeds were low, both models adequately predicted soybean seed yield reductions. When dry conditions unfavorable for crop production existed, the PRM best accommodated the interactive effects between johnsongrass, smooth pigweed, and soybean.

LAB 145 138 (LAB) was evaluated as a safener to improve sweet corn tolerance to nicosulfuron applied POST alone or with terbufos applied in the planting furrow or bentazon applied POST. To ensure enhanced injury for experimental purposes, nicosulfuron was applied at twice the registered rate alone or mixed with bentazon at the six- to seven-leaf growth stage of corn previously treated with the highest labeled rate of terbufos 15 G formulation. LAB applied as a seed treatment (ST) or POST at the two- to three-, four- to five-, or six- to seven-leaf growth stages reduced height reduction and yield loss from nicosulfuron applied POST in combination with terbufos applied in-furrow. LAB applied POST at the four- to five-leaf growth stage was most effective in preventing injury from this treatment, with yield reduced only 8% compared with 54% from the nicosulfuron and terbufos treatment. LAB applied POST at the eight- to nine-leaf growth stage did not alleviate injury. With the nicosulfuron, terbufos, and bentazon combination, LAB applied POST at the three- to four- or six- to seven-leaf growth stages decreased height reduction and yield loss caused by this combination, with LAB at the three- to four-leaf growth stage being most effective.

Field studies in conventional and vertical-tilled soybeans were conducted in 1992/93 and 1993/94 at four locations in Argentina to compare the efficacy of imazethapyr and haloxyfop for johnsongrass control. Control was evaluated by measuring frequency of johnsongrass-infested areas at the beginning of the 1992/93 and 1993/94 growing seasons. Relative johnsongrass leaf area and number of rhizome phytomers within infested areas also was evaluated. Herbicide application reduced frequency of infested areas in the 1992/93 growing season. Frequency of infested areas increased in check plots. No differences were found among herbicide treatments, suggesting that the effect of imazethapyr was as high as that observed for specific graminicides. Reduction in frequency of infested areas caused by herbicide treatments was accompanied to some extent by a decrease in intensity of infestation within infested patches assessed by measuring johnsongrass relative leaf area. Phytomer population levels, on the other hand, were reduced only after two consecutive years of herbicide application. No differences were found among herbicide treatments in terms of impact on both measures of weed abundance. Johnsongrass responded similarly to all treatments regardless of tillage system.