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The root-knot nematodes (RKN) (Meloidogyne graminicola) are a devastating threat to rice worldwide. The cultivated germplasm is either susceptible or moderately resistant to rice RKN. Therefore, there is a need to identify resistance sources against M. graminicola as an eco-friendly management strategy. The present study evaluated the host response of Oryza sativa genotypes comprising basmati, non-basmati improved varieties, their advanced breeding lines (83) and Oryza glaberrima accessions (42) against M. graminicola in the nematode-infested plot for two consecutive years. All O. sativa genotypes exhibited susceptible responses, while O. glaberrima accessions showed variable levels of resistance. Three of the O. glaberrima accessions (IRGC102196, IRGC102538 and IRGC102557) were highly resistant. M. graminicola significantly affected plant growth parameters in susceptible genotypes compared to resistant O. glaberrima accessions. The results were supported by histopathological studies that showed apparent giant cell formation in PR121 while penetration and development of M. graminicola juveniles were low in the O. glaberrima acc. IRGC102196. In silico analysis indicated that none of the reported nematode resistance genes from different crops had homology with the rice genome. The two anti-nematode genes (Oryzacystatin-I and Oryzacystatin-II) from O. sativa japonica revealed homology with O. sativa cv. PR121 and O. glaberrima acc. IRGC102206. Comparative analysis of these genes between PR121 and O. glaberrima acc. IRGC102206 resulted in the identification of SNPs/InDels that could be associated with nematode resistance. The identified SNPs/InDels could be validated, and further molecular studies are needed to provide insights into the resistance mechanism against rice RKN.
The use of repeated measures analysis of variance (ANOVA) options for the analysis of in vitro ruminal fermentation gas production profiles is illustrated. Because of the different variances and covariance structures among profile observations, ordinary ANOVA for more than two-time points is not recommended. To mitigate this problem, the Greenhouse–Geisser epsilon correction can be applied to reduce the degrees of freedom, inflated by violation of the sphericity assumption, for F ratio probability calculations. After this correction, the Box–Greenhouse–Geisser ANOVA (modified ANOVA) layout appears similar to the layout of a split-plot design ANOVA with whole plots divided into subplots (incubation time). Any F tests in the main plot part are valid but F tests involving the time factor from the subplot part need modification because time factor, by its very nature, cannot be allocated at random. Application of multivariate ANOVA, distance multivariate ANOVA, ante-dependence and mixed model analysis are also considered. All these options lend themselves to wide application in the applied biological sciences.
In this paper, we consider four scenarios for economic optimal management of a fisheries resource by a high sea and coastal fleet segment. These scenarios differ with respect to whether a common or two separate fish stocks are considered and whether the profit from land-based processing is included. The model is parametrized using the Greenland halibut fishery on the west coast of Greenland as an empirical case. For this fishery, we show that the relative ranking of the optimal high sea industry harvest and profit compared to the coastal industry harvest and profit depends on the chosen scenario. When comparing the scenarios for optimal management and the actual situation, we find that the fish stock tends to be overexploited.
The problem of classifying elliptic curves over $\mathbb Q$ with a given discriminant has received much attention. The analogous problem for genus $2$ curves has only been tackled when the absolute discriminant is a power of $2$. In this article, we classify genus $2$ curves C defined over ${\mathbb Q}$ with at least two rational Weierstrass points and whose absolute discriminant is an odd prime. In fact, we show that such a curve C must be isomorphic to a specialization of one of finitely many $1$-parameter families of genus $2$ curves. In particular, we provide genus $2$ analogues to Neumann–Setzer families of elliptic curves over the rationals.
Catecholaminergic polymorphic ventricular tachycardia is an ion channelopathy, caused by mutations in genes coding for calcium-handling proteins. It can coexist with left ventricular non-compaction. We aim to investigate the clinical and genetic characteristics of this co-phenotype.
Methods:
Medical records of 24 patients diagnosed with catecholaminergic polymorphic ventricular tachycardia in two Chinese hospitals between September, 2005, and January, 2020, were retrospectively reviewed. We evaluated their clinical and genetic characteristics, including basic demographic data, electrocardiogram parameters, medications and survival during follow-up, and their gene mutations. We did structural analysis for a novel variant ryanodine receptor 2-E4005V.
Results:
The patients included 19 with catecholaminergic polymorphic ventricular tachycardia mono-phenotype and 5 catecholaminergic polymorphic ventricular tachycardia-left ventricular non-compaction overlap patients. The median age of onset symptoms was 9.0 (8.0,13.5) years. Most patients (91.7%) had cardiac symptoms, and 50% had a family history of syncope. Overlap patients had lower peak heart rate and threshold heart rate for ventricular tachycardia and ventricular premature beat during the exercise stress test (p < 0.05). Sudden cardiac death risk may be higher in overlap patients during follow-up. Gene sequencing revealed 1 novel ryanodine receptor 2 missense mutation E4005V and 1 mutation previously unreported in catecholaminergic polymorphic ventricular tachycardia, but no left ventricular non-compaction-causing mutations were observed. In-silico analysis showed the novel mutation E4005V broke down the interaction between two charged residues.
Conclusions:
Catecholaminergic polymorphic ventricular tachycardia overlapping with left ventricular non-compaction may lead to ventricular premature beat/ventricular tachycardia during exercise stress test at lower threshold heart rate than catecholaminergic polymorphic ventricular tachycardia alone; it may also indicate a worse prognosis and requires strict follow-up. ryanodine receptor 2 mutations disrupted interactions between residues and may interfere the function of ryanodine receptor 2.
Research has demonstrated that implementation of Nocebo Hypothesis Cognitive Behavioural Therapy (NH-CBT) achieved full symptom remission in 93% of people with Functional Neurological Symptoms Disorder (FNSD), most of them exhibiting motor symptoms. The basis for NH-CBT is consistent with a predictive coding aetiological model of FNSD. This idea is transparently shared with people with FNSD in the form of telling them that their symptoms are caused by a nocebo effect, usually followed by some physical activity that aims to change the person’s belief about their body.
Aims:
To demonstrate that a version of NH-CBT can also be effective in eliminating or reducing non-epileptic seizures (assumed to be a sub-type of FNSD).
Method:
A consecutive case series design was employed. Participants were treated with NH-CBT over a 12-week period. The primary outcome measure was seizure frequency. Numerous secondary measures were employed, as well as a brief qualitative interview to explore participants’ subjective experience of treatment.
Results:
Seven out of the 10 participants became seizure free at least 2 weeks before their post-treatment assessment, and all stayed seizure-free for at least 5 months. Six of those seven remained seizure free at 6-month follow-up. There were large positive effect sizes for the majority of secondary measures assessed.
Conclusions:
This case series provides evidence of feasibility and likely utility of NH-CBT in reducing the frequency of non-epileptic seizures.
Childhood infections have been shown to stunt growth, contribute to malnutrition and reduce cognition in early adulthood. This study aimed to assess relationships between early life infections and childhood cognition at age 11 years in the Newcastle Thousand Families Study (NTFS). The analysis included 741 members from the NTFS who had complete data for infections between birth and 5 years, and the 11-plus examinations. School records from the 11-plus examinations showed cognitive (IQ), English (EQ) and arithmetic (AQ) abilities. Housing conditions, overcrowding, birth order and social class were recorded at birth. Helicobacter pylori seropositivity was measured at age 49–51 years. Multivariable linear regression was used to examine relationships between infections and cognition. The total number of infections in the first 5 years of life was not significantly associated with IQ, EQ or AQ, nor were there significant relationships between cognitive outcomes and most infections. Tonsillitis did display a positive, significant association with IQ after adjustment for confounders (b = 6.43, 95% CI 0.92, 11.94, p = 0.022). Lower respiratory tract infections (LRTIs) showed significant negative relationships with all cognitive outcomes. H. pylori seropositivity at age 50 exhibited negative, significant relationships with EQ (p = 0.014) and AQ (p = 0.024) after adjustment for confounders. Although no significant relationship between overall infections and cognition were found, there were indications that LRTIs and gastrointestinal system infections may limit cognitive development. Given these infections remain prevalent, further research regarding severity and recurrence of infections and how they affect childhood cognition is needed.
The coronavirus disease (COVID-19) pandemic necessitated alternative methods to ensure the continuity of medical education. Our study explores the efficacy and acceptability of a digital continuous medical education initiative for medical residents during this challenging period.
Methods:
From September to December 2020, 47 out of 60 enrolled trainee doctors participated in this innovative digital Continuous Medical Education (CME) approach. We utilized the Script Concordance Test to bolster clinical reasoning skills. Three simulation scenarios, namely Advanced Trauma Life Support (ATLS), Advanced Life Support (ALS), and European Paediatric Life Support (EPLS), were transformed into interactive online sessions via Zoom™. Participant feedback was also collected through a survey.
Results:
Consistent Script Concordance Testing (SCT) scores among participants indicated the effectiveness of the online training module. Feedback suggested a broad acceptance of this novel training approach. However, discrepancies observed between formative SCT scores, and summative Multiple-Choice Questions (MCQ) assessments highlighted areas for potential refinement.
Conclusions:
Our findings showcase the resilience and adaptability of medical education amidst challenges like the global pandemic. The success of methodologies such as SCT, endorsed by prestigious bodies like the European Resuscitation Council and the American Heart Association, suggests their potential in preparing health care professionals for emergent situations. This research offers valuable insights for shaping future online CME strategies.
The objective was to evaluate steer performance, meat nutritional value, land-use, food–feed competition and both economic and environmental sustainability within temperate pasture-based suckler weanling-to-beef systems with or without (forage-only) concentrates. Post-weaning, 8-month-old, late-maturing breed steers (333 kg) were assigned to one of three systems: (1) grass silage + 1.2 kg concentrate DM (148 days), followed by pasture (123 days) and finished on ad libitum concentrates (120 days) – slaughter age, 21 months (GRAIN); (2) as per (1) but pasture (196 days) and finished on grass silage ad libitum + 3.5 kg concentrate DM (124 days) – slaughter age, 24 months (SIL + GRAIN); and (3) grass silage-only (148 days), pasture (196 days), silage-only (140 days) and finished on pasture (97 days) – slaughter age, 28 months (FORAGE). The mean target carcass weight was 390 kg for each system. Data generated were used to parameterize a farm-level beef systems model. Concentrate DM intake was 1187, 606 and 0 kg/head for GRAIN, SIL + GRAIN and FORAGE, respectively. The forage-only (FORAGE) system offers several advantages, including improved farm profitability, enhanced meat fatty acid profile and only utilized inedible human feed. Consequently, associated greenhouse gas (GHG) emissions per net human edible food produced were more favourable for FORAGE. However, compared to GRAIN, the FORAGE system had an older age at slaughter and associated increased pasture land-use and GHG emissions per animal, meat weight gain and essential amino acids gain. There are therefore inevitable trade-offs, as one beef system does not improve all sustainability and GHG emission metrics.
We prove that every topologically stable homeomorphism with global attractor of $\mathbb {R}^n$ is topologically stable on its global attractor. The converse is not true. On the other hand, if a homeomorphism with global attractor of a locally compact metric space is expansive and has the shadowing property, then it is topologically stable. This extends the Walters stability theorem (Walters, On the pseudo-orbit tracing property and its relationship to stability. The structure of attractors in dynamical systems, 1978, pp. 231–244).
Most studies on path planning of robotic arm focus on obstacle avoidance at the end position of robotic arm, while ignoring the obstacle avoidance of robotic arm joint linkage, and the obstacle avoidance method has low flexibility and adaptability. This paper proposes a path obstacle avoidance algorithm for the overall 6-DOF robotic arm that is based on the improved A* algorithm and the artificial potential field method. In the first place, an improved A* algorithm is proposed to address the deficiencies of the conventional A* algorithm, such as a large number of search nodes and low computational efficiency, in robotic arm end path planning. The enhanced A* algorithm proposes a new node search strategy and local path optimization method, which significantly reduces the number of search nodes and enhances search efficiency. To achieve the manipulator joint rod avoiding obstacles, a method of robotic arm posture adjustment based on the artificial potential field method is proposed. The efficiency and environmental adaptability of the robotic arm path planning algorithm proposed in this paper are validated through three types of simulation analysis conducted in different environments. Finally, the AUBO-i10 robotic arm is used to conduct path avoidance tests. Experimental results demonstrate that the proposed method can make the manipulator move smoothly and effectively plan an obstacle-free path, proving the method’s viability.
This study aimed to assess degree of audiovestibular handicap in patients with vestibular schwannoma.
Methods
Audiovestibular handicap was assessed using the Hearing Handicap Inventory, Tinnitus Handicap Inventory and Dizziness Handicap Inventory. Patients completed questionnaires at presentation and at least one year following treatment with microsurgery, stereotactic radiosurgery or observation. Changes in audiovestibular handicap and factors affecting audiovestibular handicap were assessed.
Results
All handicap scores increased at follow up, but not significantly. The Tinnitus Handicap Inventory and Dizziness Handicap Inventory scores predicted tinnitus and dizziness respectively. The Hearing Handicap Inventory was not predictive of hearing loss. Age predicted Tinnitus Handicap Inventory score and microsurgery was associated with a deterioration in Dizziness Handicap Inventory score.
Conclusion
Audiovestibular handicap is common in patients with vestibular schwannoma, with 75 per cent having some degree of handicap in at least one inventory. The overall burden of handicap was, however, low. The increased audiovestibular handicap over time was not statistically significant, irrespective of treatment modality.
The paper presents a detailed corpus-based analysis of the German prospective stehen vor NP light verb construction. The starting point of the analysis is the claim that the construction is restricted to change-of-state nouns in the NP-internal position (Fleischhauer & Gamerschlag 2019, Fleischhauer et al. 2019). Based on corpus data, I demonstrate that although the construction shows a strong preference for such nouns, other semantic types of nouns (such as state nouns or process nouns) occur in the construction as well. I argue that process nouns in particular require contextual support to be licensed within the construction. In the paper, I present an analysis of the prospective light verb construction in terms of current relevance. This analysis accounts for the observed preference for change-of-state NP-internal nouns as well as for the need to provide contextual support for process nouns. The notion current relevance is frequently employed in the analysis of the perfect aspect; the current paper represents the first attempt to extend this notion to the prospective aspect.*
Low birthweight rats due to fetal undernutrition sustain higher corticosterone levels when exposed to stress. This is due to the upregulated expression of the pituitary-specific Gas5, a long noncoding RNA (lncRNA) that acts as a glucocorticoid receptor decoy and then competitively inhibiting the binding of glucocorticoids to DNA. However, the mechanism of Gas5 lncRNA upregulation remains unclear. Therefore, using the fetal undernourished model, we identified the factors that regulated Gas5 lncRNA expression and examined their effect on subsequent generations. We found that the expression levels of miR-23 was significantly lower in low birth-weight rats compared with controls. The expression of miR-23 was significantly lower and the expression levels of Gas5 lncRNA were significantly higher in the pituitary gland of low birth-weight offspring of the F2 and F3 generations compared with controls. The methyl modulator intervention in lactating F0 maternal rats restored miR-23 and Gas5 lncRNA expressions not only in F1, F2 and F3 offspring. Moreover, the intervention reduced circulating corticosterone levels and gene expressions in the pituitary gland after restraint stress exposure. In conclusion, miR-23-mediated alterations of the stress response are inherited and restored by methyl modulator intervention during lactation.