The silkworm Bombyx mori (Lepidoptera: Bombycidae) is a lepidopteran model insect of great economic importance. The parasitoid Exorista sorbillans (Diptera, Tachinidae) is the major pest of B. mori and also a promising candidate for biological control. However, the molecular interactions between hosts and dipteran parasitoids have only partially been studied. Gene expression analysis by reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) is indispensable to characterise their interactions. Accurate normalisation of RT-qPCR-based gene expression requires the use of reference genes that are constantly expressed irrespective of experimental conditions. In this study, the expression stability of 13 traditionally used reference genes was estimated by five statistical algorithms (ΔCt, geNorm, Normfinder, BestKeeper, and RefFinder) to determine the best reference genes for gene expression studies in different tissues of B. mori under E. sorbillans parasitism. Specifically, TATA-box-binding protein was the best reference gene in epidermis and testis, while elongation factor 1α was the most stable gene in prothoracic gland and midgut. Elongation factor 1γ, ribosomal protein L3, actin A1, ribosomal protein L40, glyceraldehyde-3-phosphate dehydrogenase and eukaryotic translation initiation factor 4A were the most suitable genes in head, silk gland, fat body, haemolymph, Malpighian tubule and ovary, respectively. Our study offers a set of suitable reference genes for gene expression normalisation in B. mori under the parasitic stress of E. sorbillans, which will benefit the in-depth exploration of host-dipteran parasitoid interactions, and also provide insights for further improvements of B. mori resistance against parasitoids and biocontrol efficacy of dipteran parasitoids.

While Republicans enjoyed unified control of the national government during the 1920s, scandals involving executive patronage and GOP state bosses in the South dogged the national party throughout the decade. The Republican Party in the South had been a set of “rotten boroughs” for decades, used by national politicians—especially presidents—for the sole purpose of controlling delegates at the Republican National Convention. This patronage-for-delegates arrangement was generally understood among political elites, but the murder-suicide involving a U.S. postmaster in Georgia in April 1928 brought the Southern GOP’s patronage practices to national light. This forced Republican leaders in an election year to call for a Senate investigation. Chaired by Sen. Smith W. Brookhart (R-IA), the committee investigation lasted for eighteen months, covered portions of two Republican presidential administrations, and showed how state GOP leaders in Georgia, Mississippi, South Carolina, and Texas engaged in office selling. The fallout would be a thorn in the side of President Herbert Hoover, who tried to clean up the corrupt GOP organizations in the South—and build an electorally-viable Republican Party in the ex-Confederate states—but largely failed.

Vitrification has been widely used for oocyte cryopreservation, but there is still a need for optimization to improve clinical outcomes. In this study, we compared the routine droplet merge protocol with modified multi-gradient equilibration vitrification for cryopreservation of mouse oocytes at metaphase II. Subsequently, the oocytes were thawed and subjected to intracytoplasmic sperm injection (ICSI). Oocyte survival and spindle status were evaluated by morphology and immunofluorescence staining. Moreover, the fertilization rates and blastocyst development were examined in vitro. The results showed that multi-gradient equilibration vitrification outperformed droplet merge vitrification in terms of oocyte survival, spindle morphology, blastocyst formation, and embryo quality. In contrast, droplet merge vitrification exhibited decreasing survival rates, a reduced proportion of oocytes with normal spindle morphology, and lower blastocyst rates as the number of loaded oocytes increased. Notably, when more than six oocytes were loaded, reduced oocyte survival rates, abnormal oocyte spindle morphology, and poor embryo quality were observed. These findings highlight that the vitrification of mouse metaphase II oocytes by the modified multi-gradient equilibration vitrification has the advantage of maintaining oocyte survival, spindle morphology, and subsequent embryonic development.

Habitat selection of Culicoides spp. (Diptera: Ceratopogonidae) is influenced by the physicochemical factors such as temperature, pH, salinity, moisture, conductivity, organic and inorganic compounds of substrates. These factors determine the life history traits of the vectors. We studied the influence of substrate salinity (0–40 parts per thousand, ppt) and pH (pH 1–13) on oviposition, egg hatching, larval survivability, and adult emergence of Culicoides peregrinus Kieffer under laboratory conditions. Most eggs (80.74%) were laid in 0 ppt and 95% in pH 7 but lowered with increased salinity and pH levels. It was observed that the females did not lay eggs in 30 ppt to 40 ppt salinity; pH 1 and pH 13 but interestingly up to 95% of the eggs were retained within the abdomen. Little effect of salinity and pH on egg hatching was observed up to 5 ppt and 10 ppt except at the extreme values of 40 ppt and pH 1, pH 13. Pupation did not occur in rearing plates with high salinities, 30 ppt and 40 ppt, although the few eggs hatched when exposed to such salinity. In low salinity (0 to 2 ppt), occurrence of adult emergence was more and then decreased with increasing salinity. Maximum emergence was seen when the rearing media was alkaline. This study deals with the suitability of breeding substrate of C. peregrinus when exposed to salinity and pH ranges. Our study suggests the ambient salinity and pH ranges to be maintained during laboratory rearing of this vector species.

The present study investigates several linguistic factors that might be determinants of differences in the ease of acquisition of relative clauses (RCs) in L2 contexts, using the acquisition of English relative clauses by Kurdish Sorani native speakers. The analysis of 1440 subject and object RCs formed by Kurdish Sorani-speaking learners of English in a sentence combination task and a sentence translation task indicates that the syntactic functions of the noun phrase (NP) relativized in the RC, the position of the RC in the matrix clause (whether centre- or right-embedded), and the properties of RCs in L1 affect the acquisition and the formation of RCs in L2. The study also provides evidence that the syntactic functions of the relativized NP in the matrix clause, and the consistency/inconsistency of word orders in L1 and L2 do not influence the acquisition of RCs in L2.

Climate factors, pesticides, and landscape in coffee agroecosystems directly affect the populations of the coffee leaf miner and its parasitoids. This study aimed to investigate the effects of climate factors, insecticide use, and landscape on natural parasitism, parasitoid diversity, and infestation of L. coffeella in coffee plantations in the Planalto region, Bahia, Brazil. Mined leaves were collected monthly in six coffee plantations with varying edge density, vegetation cover, landscape diversity in scales of 500 to 3000 m of radius, insecticide use, and climate factors. Closterocerus coffeellae, and Proacrias coffeae (Eulophidae) predominated in the pest's natural parasitism. Our record is the first for the occurrence of Stiropius reticulatus, Neochrysocharis sp. 1, Neochrysocharis sp. 2, and Zagrammosoma sp. in Bahia. Higher temperature and larger forest cover increased the coffee leaf miner infestation. Higher rainfall values, insecticide use, and landscape diversity decreased the pest infestations. Natural parasitism and species diversity are favoured by increase in temperature, forest cover, and edge density, while increase in rainfall, insecticide use, and landscape diversity lead them to decrease.The natural parasitism and diversity of parasitoid species of the coffee leaf miner have been enhancing in the areas with greater forest cover and edge density associated with low use of insecticides. The areas composed of different lands with annual croplands surrounding the coffee plantations showed less natural parasitism and parasitoid species diversity. The ecosystem services provided by C. coffeellae and P. coffeae in coffee crops areas require conservation and these species are potential bioproducts for applied biological control programmes.

In this article, we document the gender of the noun “COVID-19” in a database of more than 76,000 tweets and in traditional media (approximately 500,000 articles) in French as spoken in Africa, (North) America and Europe. We find that North American media comply near-categorically with the recommendations of the feminine by the World Health Organization and local linguistic authorities in March 2020. The majority of North American tweets follow suit soon after. The African data show an increase of articles and tweets adopting the feminine after the Académie française's recommendation in May 2020. Finally, the feminine is negligible in the European data. We argue that among the factors at play are dialect-specific differences in French gender and loanword adaptation; the complex relationship among linguistic authorities, the public, and local media; and the relative delay in the Académie française's recommendation of the feminine.

The role that China is perceived to present for the global economy—much less global security—has changed remarkably since China’s accession to the World Trade Organization (WTO) in December 2001. During the height of neoliberalism and third-wave democracies, China appeared more open to foreign capital than the developmental states of East Asia, which had restricted foreign direct investment (FDI) in favor of domestic industrialization during a comparable stage of economic development. Through the first 15 years of China’s WTO membership, many in academia and the policy world depicted a modernizing China undergoing market transition, in parallel to Eastern European countries after the collapse of the Soviet Union.

The US-China relationship has worsened dramatically in recent years. After decades of pro-engagement policies toward China, a bipartisan consensus began to emerge around 2016 that engagement had neither accomplished US policy goals (such as encouraging China to liberalize politically) nor served US citizens well.1 At the same time, political changes within China have pointed to a declining appetite for engagement with the United States and sidelined the domestic constituencies who remained interested in cooperation and exchange. Further restrictions have shrunk the already limited space for media, civil society groups, and academic exchange in China. Pandemic-related border closures eliminated in-person exchanges almost completely for nearly three years. And some are concerned that China’s 2021 Personal Information Protection Law could be used to prosecute foreign researchers who conduct research in China (Lewis 2023).