This paper investigates the effects of a light-actuated photosurfactant on the canonical problem of the linear stability of a viscous thread surrounded by a dynamically passive fluid. A model consisting of the Navier–Stokes equations and a set of molar concentration equations is presented that capture light-induced switching between two stable surfactant isomer states, trans and cis. These two states display significantly different interfacial properties, allowing for some external control of the stability behaviour of the thread via incident light. Normal modes are used to generate a generalized eigenvalue problem for the growth rate which is solved with a hybrid analytical and numerical method. The results are validated with appropriate analytical solutions of increasing complexity, beginning with a solution to a clean interface, then analytical solutions for one insoluble surfactant, one soluble surfactant and a special case of two photosurfactants with a spatially uniform undisturbed state. Presenting each of these cases allows for a holistic discussion of the effect of surfactants in general on the stability of a liquid thread. Finally, the numerical solutions in the presence of two photosurfactants that display radially non-uniform undisturbed states are presented, and details of the impact of the illumination on the linear stability of the thread are discussed.

It is known that an increased flow rate can be achieved in channel flows when smooth walls are replaced by superhydrophobic surfaces. This reduces friction and increases the flux for a given driving force. Applications include thermal management in microelectronics, where a competition between convective and conductive resistance must be accounted for in order to evaluate any advantages of these surfaces. Of particular interest is the hydrodynamic stability of the underlying basic flows, something that has been largely overlooked in the literature, but is of key relevance to applications that typically base design on steady states or apparent-slip models that approximate them. We consider the global stability problem in the case where the longitudinal grooves are periodic in the spanwise direction. The flow is driven along the grooves by either the motion of a smooth upper lid or a constant pressure gradient. In the case of smooth walls, the former problem (plane Couette flow) is linearly stable at all Reynolds numbers whereas the latter (plane Poiseuille flow) becomes unstable above a relatively large Reynolds number. When grooves are present our work shows that additional instabilities arise in both cases, with critical Reynolds numbers small enough to be achievable in applications. Generally, for lid-driven flows one unstable mode is found that becomes neutral as the Reynolds number increases, indicating that the flows are inviscidly stable. For pressure-driven flows, two modes can coexist and exchange stability depending on the channel height and slip fraction. The first mode remains unstable as the Reynolds number increases and corresponds to an unstable mode of the two-dimensional Rayleigh equation, while the second mode becomes neutrally stable at infinite Reynolds numbers. Comparisons of critical Reynolds numbers with the experimental observations for pressure-driven flows of Daniello et al. (Phys. Fluids, vol. 21, issue 8, 2009, p. 085103) are encouraging.

Zolpidem is a GABA (A) agonist, which is indicated for the short-term management of insomnia. Recent research provide evidence suggesting that zolpidem produces spatial working memory (WM) deficits and dependence; however, the underlying mechanisms of these effects are unknown. Since the auditory N400 component of event-related potentials (ERPS) is considered as an index of memory use of context processing, the present study focused on N400 waveform of ERPs elicited during a WM task in a case suffering from zolpidem dependence. The patterns of N400 waveform of this case were compared to the patterns obtained from healthy controls. This comparison revealed that zolpidem dependence is accompanied by reduced amplitudes located at posterior brain areas and diffuse prolongation of N400. These findings may indicate that zolpidem dependence manifests alterations with regard to the memory use of context processing, involving or affecting a wide-ranging network of the brain's structures.

Many association studies have reported associations between the brain-derived neurotrophic factor (BDNF) gene and psychiatric disorders including major depression (MDD). the BDNF gene has further been associated with suicidal behaviour, as well as with treatment response, although with conflicting results. in the present study, we further elucidate the impact of BDNF in MDD patients with suicide risk and/or a personal history of suicide attempts.

Two hundred fifty MDD patients were collected in the context of a European multicentre resistant depression study and treated with antidepressants at adequate doses for at least 4 weeks. Suicidality was assessed using Mini International Neuropsychiatric Interview (MINI) and Hamilton Rating Scale for Depression (HAM-D). Treatment response was defined as HAM-D ≤ 17 and remission as HAM-D ≤ 7 after 4 weeks of treatment with antidepressants at adequate dose. Genotyping was performed for eight SNPs within the BDNF gene.

With regard to suicide risk and personal history of suicide attempts, neither single marker nor haplotypic association was found with any SNP after multiple testing correction. However, in gender-specific analyses, we found haplotypic association with suicide risk in males, but not in females (rs925946-rs10501087-rs6265, rs10501087-rs6265-rs122733). the only single-marker association with suicide risk in males (rs908867) did not resist multiple testing correction. No significant associations were found in gender-specific analyses with regard to a personal history of suicide attempts.

In conclusion, we found two BDNF haplotypes significantly associated with suicide risk in male MDD patients. However, replication in larger well-defined cohorts is required to dissect this further.

Malnutrition risk screening in cirrhotic patients is crucial, as poor nutritional status negatively affects disease prognosis and survival. Given that a variety of malnutrition screening tools is usually used in routine clinical practice, the effectiveness of eight screening tools in detecting malnutrition risk in cirrhotic patients was sought. A total of 170 patients (57·1 % male, 59·4 (sd 10·5) years, 50·6 % decompensated ones) with cirrhosis of various aetiologies were enrolled. Nutritional screening was performed using the Malnutrition Universal Screening Tool, Nutritional Risk Index, Malnutrition Screening Tool, Nutritional Risk Screening (NRS-2002), Birmingham Nutritional Risk Score, Short Nutritional Assessment Questionnaire, Royal Free Hospital Nutritional Prioritizing Tool (RFH-NPT) and Liver Disease Undernutrition Screening Tool (LDUST). Malnutrition diagnosis was defined using the Subjective Global Assessment (SGA). Data on 1-year survival were available for 145 patients. The prevalence of malnutrition risk varied according to the screening tools used, with a range of 13·5–54·1 %. RFH-NPT and LDUST were the most accurate in detecting malnutrition (AUC = 0·885 and 0·892, respectively) with a high sensitivity (97·4 and 94·9 %, respectively) and fair specificity (73·3 and 58 %, respectively). Malnutrition according to SGA was an independent prognostic factor of within 1-year mortality (relative risk was 2·17 (95 % CI 1·0, 4·7), P = 0·049) after adjustment for sex, age, disease aetiology and Model for End-stage Liver Disease score, whereas nutrition risk according to RFH-NPT, LDUST and NRS-2002 showed no association. RFH-NPT and LDUST were the only screening tools that proved to be accurate in detecting malnutrition in cirrhotic patients.

Lifestyle interventions remain the cornerstone therapy for non-alcoholic fatty liver disease (NAFLD). This randomised controlled single-blind clinical trial investigated the effect of Mediterranean diet (MD) or Mediterranean lifestyle, along with weight loss, in NAFLD patients. In all, sixty-three overweight/obese patients (50 (sd 11) years, BMI=31·8 (sd 4·5) kg/m2, 68 % men) with ultrasonography-proven NAFLD (and elevated alanine aminotransferase (ALT) and/or γ-glutamyl transpeptidase (GGT) levels) were randomised to the following groups: (A) control group (CG), (B) Mediterranean diet group (MDG) or (C) Mediterranean lifestyle group (MLG). Participants of MDG and MLG attended seven 60-min group sessions for 6 months, aiming at weight loss and increasing adherence to MD. In the MLG, additional guidance for increasing physical activity and improving sleep habits were given. Patients in CG received only written information for a healthy lifestyle. At the end of 6 months, 88·8 % of participants completed the study. On the basis of intention-to-treat analysis, both MDG and MLG showed greater weight reduction and higher adherence to MD compared with the CG (all P<0·05) at the end of intervention. In addition, MLG increased vigorous exercise compared with the other two study groups (P<0·001) and mid-day rest/naps compared with CG (P=0·04). MLG showed significant improvements in ALT levels (i.e. ALT<40 U/l (P=0·03) and 50 % reduction of ALT levels (P=0·009)) and liver stiffness (P=0·004) compared with CG after adjusting for % weight loss and baseline values. MDG improved only liver stiffness compared with CG (P<0·001) after adjusting for the aforementioned variables. Small changes towards the Mediterranean lifestyle, along with weight loss, can be a treatment option for patients with NAFLD.

The nonlinear stability of viscous, immiscible multilayer flows in plane channels driven both by a pressure gradient and gravity is studied. Three fluid phases are present with two interfaces. Weakly nonlinear models of coupled evolution equations for the interfacial positions are derived and studied for inertialess, stably stratified flows in channels at small inclination angles. Interfacial tension is demoted and high-wavenumber stabilisation enters due to density stratification through second-order dissipation terms rather than the fourth-order ones found for strong interfacial tension. An asymptotic analysis is carried out to demonstrate how these models arise. The governing equations are $2\times 2$ systems of second-order semi-linear parabolic partial differential equations (PDEs) that can exhibit inertialess instabilities due to interaction between the interfaces. Mathematically this takes place due to a transition of the nonlinear flux function from hyperbolic to elliptic behaviour. The concept of hyperbolic invariant regions, found in nonlinear parabolic systems, is used to analyse this inertialess mechanism and to derive a transition criterion to predict the large-time nonlinear state of the system. The criterion is shown to predict nonlinear stability or instability of flows that are stable initially, i.e. the initial nonlinear fluxes are hyperbolic. Stability requires the hyperbolicity to persist at large times, whereas instability sets in when ellipticity is encountered as the system evolves. In the former case the solution decays asymptotically to its uniform base state, while in the latter case nonlinear travelling waves can emerge that could not be predicted by a linear stability analysis. The nonlinear analysis predicts threshold initial disturbances above which instability emerges.

We consider the full three-dimensional dynamics of a thin falling liquid film on a flat plate inclined at some non-zero angle to the horizontal. In addition to gravitational effects, the flow is driven by an electric field which is normal to the substrate far from the flow. This extends the work of Tseluiko & Papageorgiou (J. Fluid Mech., vol. 556, 2006b, pp. 361–386) by including transverse dynamics. We study both the cases of overlying and hanging films, where the liquid lies above or below the substrate, respectively. Starting with the Navier–Stokes equations coupled with electrostatics, a fully nonlinear two-dimensional Benney equation for the interfacial dynamics is derived, valid for waves that are long compared to the film thickness. The weakly nonlinear evolution is governed by a Kuramoto–Sivashinsky equation with a non-local term due to the electric field effect. The electric field term is linearly destabilising and produces growth rates proportional to $|\unicode[STIX]{x1D743}|^{3}$ , where $\unicode[STIX]{x1D743}$ is the wavenumber vector of the perturbations. It is found that transverse gravitational instabilities are always present for hanging films, and this leads to unboundedness of nonlinear solutions even in the absence of electric fields – this is due to the anisotropy of the nonlinearity. For overlying films and a restriction on the strength of the electric field, the equation is well-posed in the sense that it possesses bounded solutions. This two-dimensional equation is studied numerically for the case of periodic boundary conditions in order to assess the effects of inertia, electric field strength and the size of the periodic domain. Rich dynamical behaviours are observed and reported. For subcritical Reynolds number flows, a sufficiently strong electric field can promote non-trivial dynamics for some choices of domain size, leading to fully two-dimensional evolutions of the interface. We also observe two-dimensional spatiotemporal chaos on sufficiently large domains. For supercritical flows, such two-dimensional chaotic dynamics emerges in the absence of a field, and its presence enhances the amplitude of the fluctuations and broadens their spectrum.

We present a model for ice formation in a thin, viscous liquid film driven by a Blasius boundary layer after heating is switched off along part of the flat plate. The flow is assumed to initially be in the Nelson et al. (J. Fluid Mech., vol. 284, 1995, pp. 159–169) steady-state configuration with a constant flux of liquid supplied at the tip of the plate, so that the film thickness grows like $x^{1/4}$ in distance along the plate. Plate cooling is applied downstream of a point, $Lx_{0}$, an $O(L)$-distance from the tip of the plate, where $L$ is much larger than the film thickness. The cooling is assumed to be slow enough that the flow is quasi-steady. We present a thorough asymptotic derivation of the governing equations from the incompressible Navier–Stokes equations in each fluid and the corresponding Stefan problem for ice growth. The problem breaks down into two temporal regimes corresponding to the relative size of the temperature difference across the ice, which are analysed in detail asymptotically and numerically. In each regime, two distinct spatial regions arise, an outer region of the length scale of the plate, and an inner region close to $x_{0}$ in which the film and air are driven over the growing ice layer. Moreover, in the early time regime, there is an additional intermediate region in which the air–water interface propagates a slope discontinuity downstream due to the sudden onset of the ice at the switch-off point. For each regime, we present ice profiles and growth rates, and show that for large times, the film is predicted to rupture in the outer region when the slope discontinuity becomes sufficiently enhanced.

DNA methylation is an epigenetic marker that has been shown to vary significantly across different tissues. Taking advantage of the methylation differences between placenta-derived cell-free DNA and maternal blood, several groups employed different approaches for the discovery of fetal-specific biomarkers. The aim of this study was to analyse whole-genome fetal and maternal methylomes in order to identify and confirm the presence of differentially methylated regions (DMRs). We have initially utilized methylated DNA immunoprecipitation (MeDIP) and next-generation sequencing (NGS) to identify genome-wide DMRs between chorionic villus sampling (CVS) and female non-pregnant plasma (PL) and peripheral blood (WBF) samples. Next, using specific criteria, 331 fetal-specific DMRs were selected and confirmed in eight CVS, eight WBF and eight PL samples by combining MeDIP and in-solution targeted enrichment followed by NGS. Results showed higher enrichment in CVS samples as compared to both WBF and PL samples, confirming the distinct methylation levels between fetal and maternal DNA for the selected DMRs. We have successfully implemented a novel approach for the discovery and confirmation of a significant number of fetal-specific DMRs by combining for the first time MeDIP and in-solution targeted enrichment followed by NGS. The implementation of this double-enrichment approach is highly efficient and enables the detailed analysis of multiple DMRs by targeted NGS. Also, this is, to our knowledge, the first reported application of MeDIP on plasma samples, which leverages the implementation of our enrichment methodology in the detection of fetal abnormalities in maternal plasma.