Vitamin A deficiency (VAD) is a significant public health problem in manycountries. While cost-effective interventions are available to control VAD,reliable information is needed to the track progress of control programmes.However, assessment of VAD is uncommon because current approaches are expensiveand not feasible in low-resource settings. The present study explores theutility of retinol-binding protein (RBP), analysed by enzyme-linkedimmunosorbent assay from capillary blood, as an alternative measure of serumretinol concentrations in populations. The study collected matched panels ofvenous and capillary blood from pre-school children in Chiang Mai, Thailand. Ofa total sample of 195 children, there were no differences between RBP fromvenous blood, RBP from capillary blood or retinol from capillary blood relativeto retinol from venous blood. Receiver-operating characteristic curve analysissuggested a cut-off of RBP < 0.825 μmoll−1 had optimal screening proficiency relative toretinol <0.70 μmol l−1. For thepurpose of population assessment, all three parameters performed well inscreening for VAD relative to retinol from venous blood. There were nodifferences in the estimates of VAD between children stratified by inflammationstatus. Lower RBP concentrations were found in children in the earlyconvalescent stage of infection than in children with no infection or in thelate convalescent stage. This study provided evidence of the biologicalcomparability between retinol and RBP estimated from venous blood and capillaryblood. This is a critical observation as it provides empirical evidence that RBPfrom capillary blood is a surrogate measure of serum retinol concentrations.