This study examines Neltuma pallida wood from the Cerro Laguna archaeological site in coastal Peru to establish a floating chronology and absolute dating. Ten cross-sections yielded a 70-year chronology (CLAA1). Alpha-cellulose was extracted from five single growth rings from one sample for radiocarbon dating, then wiggle-matching in OxCal v4.4 using SHCal20. Results place the youngest ring at 1314–1330 CE (95.4% probability), situating the full sequence in the period between 1253 and 1322 (±8) CE. Statistical analyses show a robust dendrochronological signal, and ring-width data suggest strong El Niño Southern Oscillation (ENSO) events around 1300 CE. These findings confirm Cerro Laguna’s Late Intermediate Period occupation and allow us to assume that the entire 70-year period can be used to improve the calibration curve for the Southern Hemisphere.

The worldwide adoption of automatic milking systems (AMSs) has expanded, attracting growing interest from researchers. Although several reviews have already been carried out regarding the features of AMS in confined systems, a comprehensive bibliometric analysis of the literature on automated milking in pasture-based systems is still lacking. Therefore, this review set out to provide a bibliometric approach focused on pasture-based AMS to explore and map the extent of research in the scientific literature. Initially, a comprehensive search was carried out in the Web of Science and Scopus databases. Then, a four-step PRISMA evaluation was applied on 235 identified articles, resulting in a final sample of 45 articles. Most studies were conducted in Europe (44%), in research herds (79%), involving Holstein-Friesian cows (76%), using automated milking from Lely (57%) and DeLaval brands (30%). White clover and perennial and annual ryegrass are the most commonly used pastures among the countries (87.5% vs. 75%). The main topics (33%) investigated over studies were milk production, composition and efficiency of AMS on pasture, followed by animal behaviour (27%), animal nutrition (16%), heat stress (9%), methane emissions (7%), health disorders (2%), economic viability (2%), somatic cell count and mastitis (2%) and energy consumption (2%). Concentrate allocation and heat stress were identified as emerging research areas that deserve greater attention in future studies. This study contributes to our understanding of scientific developments, trends and research gaps regarding pasture-based AMSs.

At ANSTO, we have developed an automated dissolved inorganic carbon (DIC) processing line, known as A-DIC, designed to sequentially process 10 water samples for radiocarbon analysis without any operator intervention. The A-DIC works at ambient pressure using helium (He) gas as a carrier. It includes a water pump capable of drawing adjustable sample volumes between 22-180 mL into a 250 mL reaction vessel. Upon the addition of 5 mL of phosphoric acid to the vessel, the sample is sparged with helium (He) gas at a rate of 45 mL/min for 16 min. The desorbed CO2 from the sample is then carried along with He through a series of traps: water vapor traps at –75 °C, a sulphur (SOx) trap containing silver wires at 650 °C, and two CO2 traps at –196 °C. The A-DIC is automated via a Python script executed on a PC connected via USB. It demonstrates consistent performance and CO2 trapping efficiency across batches of up to 10 samples. For contamination study, the CO2 gas collected is transferred to graphitization units to be converted into graphite for AMS analysis.

Radiocarbon dating by accelerator mass spectrometry (AMS) requires transforming samples into graphite, a step that typically depends on liquid nitrogen and high-purity carrier gases, increasing both cost and operational complexity. We present μGRAPHILINE, a fully automated, modular combustion–graphitization line that removes these dependencies by combining dual-zone combustion with iron–zinc reduction. Performance was evaluated on more than 180 standards and reference materials targets measured on two independent AMS systems. The μGRAPHILINE consistently achieved >90% graphitization yields, stable backgrounds of ∼0.24 pMC (≈48,500 BP), and sample throughput of ∼3.5 hours per target. Reliable operation was demonstrated for a broad sample-size range, from routine 1 mg C down to ∼0.2 mg C, with ion currents sufficient for precise AMS analysis. The system’s automation, modularity, and low memory effect support improved laboratory throughput and operator efficiency. These results indicate that μGRAPHILINE provides a robust and efficient approach to radiocarbon sample preparation with broad potential applications.

In Brazil, laws are being adopted prohibiting the use and distribution of non-recyclable plastic bags and encouraging the use of recyclable, biodegradable and bioplastic bags. However, there is no specific standard method in Brazil to inspect the carbon content of bioplastics. This work aims to implement the sample preparation for radiocarbon biogenic fraction determination in bioplastics in the new radiocarbon laboratory at the Federal University of Bahia (LAPA14C-UFBA). Six plastic samples from Brazilian markets with bio-based seals were collected in regular Brazilian markets, four bags and two tubes. All samples were combusted and graphitized in the LAPA14C-UFBA and the graphites were measured at the Radiocarbon Laboratory (LAC-UFF). The biogenic fraction analysis followed the Standard Test Methods for Determining the Biobased Content of Solid, Liquid, and Gaseous Samples Using Radiocarbon Analysis (ASTM D6866-24). Since the bioplastic manufactured in Brazil is derived from sugarcane, a C4 plant where δ13C values are different from those of petroleum-derived plastic, the biogenic fraction was obtained via the isotopic mixture equation. The results showed a robust direct correlation (r=0.998) between the fractions obtained by the two methods, indicating that δ13C analysis can be used, in this case, as an alternative in determining the biogenic fraction of plastics. From the results obtained, four out of the six samples analyzed showed biogenic fraction values below the 51% required by the I’m Green label, with values ranging from 4% to 43%, according to the δ3C and 14C analysis, showing the efficiency of both techniques in determining the biogenic fraction.

Within a collaboration between the Brazilian Federal Police and the Radiocarbon Laboratory of the Fluminense Federal University (LAC-UFF), this work studies seized art objects made from ivory. We aim to develop protocols to verify whether they are illegal according to the Convention on International Trade of Endangered Species from Wild Flora and Fauna (CITES) law by measuring the carbon-14 concentration in the modified ivory samples from different sampling spots and comparing it to the bomb peak curve. Over the course of this research, we evaluate the uncertainties related to the determination of the elephants’ death. These uncertainties are due to several factors such as the provenance of the elephants, growth pattern of the tusks and incorporation of atmospheric radiocarbon to the tissues, sampling methods of ivory objects of different sizes and shapes, and radiocarbon data analysis. This work is a pioneer study in Brazil and is likely to become a reference in the country in the field of radiocarbon analyses in forensic contexts.

The hypothesis of Paleolithic rock art in Siberia remains neither proven nor disproven. This research provides key evidence for a later age of the earliest known images in the Minusinsk Depression. A set of geological, geomorphological, and absolute-dating methods was utilized to reconstruct the geological history of the Maydashy rock art site (Minusinsk Depression, Southern Siberia). This research for the first time has allowed us to date the lower chronological boundary of the period when the earliest images known in Southern Siberia as “Minusinsk Style” were created. In the Late Pleistocene, the cliff area of the site was buried under alluvial deposits of the first floodplain terrace of the Yenisei River that formed in the second half of MIS 2. At the beginning of the Holocene, before its optimal onset, Yenisei eroded the terrace and exposed the rock surface to mark the potential lower boundary (< 11 ka) when the Maydashy site images were carved. This approach can be useful in rock art research for dating carvings from open-air sites; it is applicable for the rock art sites of other regions where ravine formation affected their natural context.

The Cunene region of southern Angola, especially the area around Xangongo, hosts a large number of African baobabs, including several superlative specimens. Our research reports the investigation of the three biggest specimens from Xangongo Grove, named XG-1 (11 stems; circumference 26.34 m), XG-2 (5 stems; 22.70 m) and XG-3 (9 stems; 27.73 m), and of the largest baobab from Xangongo town, named XT-1 (7 stems; 21.02 m). Several wood samples were collected from these four baobabs mainly as cores from trunks and/or primary branches and then radiocarbon-dated by AMS. The two oldest samples were extracted from two primary branches of baobab XG-1. These have practically identical radiocarbon dates of 1822 ± 19 BP and 1822 ± 10 BP, which correspond to identical calibrated ages of 1785 ± 15 and 1785 ± 10 calendar years. The calculated age of baobab XG-1 is 2100 ± 50 years. Thus, XG-1, which is called by the locals “The biggest baobab of Africa”, becomes the oldest living African baobab with accurate dating results. The results indicate ages of 1100 years for baobab XG-2, 850 years for baobab XG-3 and 550 years for baobab XT-1.

Although radiocarbon-accelerator mass spectrometry (14C-AMS) is an important tool for the establishment of soil chronology, its application is challenging due to the complex nature of soil samples. In the present study, chemical extraction methodologies were tested to obtain the most representative age of Amazonian soil deposition by 14C-AMS. We performed acid hydrolysis with different numbers of extractions, as well as treatments combining acid and bases and quartered and non-quartered samples. The ages of the soil organic matter (SOM) fractions were compared to the ages of naturally buried charcoal samples at similar depths. The results showed that the age of the non-hydrolyzable inert fraction of soil was closer to the age of charcoal and older than the ages of humin. It was also observed that the quartering process can influence the results, since the dating of the humin fraction showed variability in the results. Our results are important to provide information about the most suitable method for the 14C-AMS dating of soil samples for paleoenvironment reconstruction studies.

Radiocarbon (14C) measurements on dissolved inorganic carbon (DIC) are a powerful tool to trace water masses and carbon cycling in the ocean. Existing methodologies to determine the 14C content of seawater DIC requires large volumes of sample (usually >100 mL) and specialized graphitization techniques to achieve the accuracy and precision needed for meaningful data interpretation. The advancement of the CO2 gas ionization accelerator mass spectrometry (AMS) technique today allows routine 14C measurements on small samples (<100 µgC) and may thus permit reducing the sample volumes needed to determine 14C content of seawater DIC to ∼2 mL. The proposed method utilizes the carbonate handling system (CHS), gas interface system (GIS) and MICADAS AMS, and provides good accuracy but reduced precision compared to established methods. Good accuracy is shown by comparing results for a marine in-house DIC standard and a DIC seawater profile from Antarctica between the proposed CHS-GIS-MICADAS approach and reference measurements conducted on the same material at established laboratories (ETH and NOSAMS). Further, two sedimentary porewater profiles from a fjord system in Svalbard are presented. Despite good agreement, the precision of the CHS-GIS-MICADAS approach is reduced, potentially limiting possible interpretations on seawater DIC. Nonetheless, the reduction of sample volumes proves particularly helpful to analyze porewater DIC from sediment cores, where sample material is notoriously limited, reduces the required amounts of toxic HgCl2 and simplifies expedition logistics.

Accelerator mass spectrometry (AMS) is a worldwide recognized method for radiocarbon (14C) dating. The advantageous aspects of this method include the variety of materials and the small sample size (1 mg of carbon) that can be measured. However, these pose several challenges in the laboratory, such as developing appropriate chemical pretreatment methods. In the summer of 2022, the Radiocarbon and Mass Spectrometry Laboratory in Gliwice, Poland, launched the MICADAS accelerator spectrometer. The report on background and reference materials measurement results for the period from September 2022 to July 2024 is presented in this publication. Quality assurance and quality control processes are extremely important to guarantee the high quality of the results obtained in the laboratory. Hence, our Radiocarbon and Mass Spectrometry Laboratory in Gliwice took part in the Glasgow International Radiocarbon Inter-Comparison (GIRI) program. The radiocarbon ages for wood, bone, humic acid, and barley mash samples were determined and compared with reported values. The resulting data confirmed that our Laboratory is capable of dating samples across a spectrum of materials and ages ranging from contemporary to the limits of the radiocarbon method, achieving precision on par with that of other laboratories.

A routine chemical procedure was developed at the Ede Hertelendi Laboratory of Environmental Studies (HEKAL), in Debrecen which can measure the dissolved organic radiocarbon content of groundwater as well as the inorganic and total fraction. The typical background of this non-purgeable dissolved organic radiocarbon preparation is 0.73 ± 0.14 percent modern carbon (pMC), using a carbon contamination correction on fossil dissolved material (potassium hydrogen phthalate) samples.

As a required sample preparation method for 14C graphite, the Zn-Fe reduction method has been widely used in various laboratories. However, there is still insufficient research to improve the efficiency of graphite synthesis, reduce modern carbon contamination, and test other condition methodologies at Guangxi Normal University (GXNU). In this work, the experimental parameters, such as the reduction temperature, reaction time, reagent dose, Fe powder pretreatment, and other factors, in the Zn-Fe flame sealing reduction method for 14C graphite samples were explored and determined. The background induced by the sample preparation process was (2.06 ± 0.55) × 10–15, while the 12C– beam current were better than 40μA. The results provide essential instructions for preparing 14C graphite of ∼1 mg at the GXNU lab and technical support for the development of 14C dating and tracing, contributing to biology and environmental science.

The increase in biobased material usage requires the methods of verification to investigate the actual content of biocarbon in such materials, including liquid fuels. The determination of biocarbon in liquid samples using 14C required adaptation of existing sample preparation methods. In this study, both accelerator mass spectrometry (AMS) and liquid scintillation counting (LSC) methods were used to determine the content of 14C in six different liquid fuel blends produced from purely bio-based hydrotreated vegetable oil (HVO) and a 14C-free petrodiesel sample (ON/UF-BC). The results obtained for pure petrodiesel provide background values. The results indicate a good agreement between LSC and AMS, and a linear correlation between the 14C measurement results for blended samples and HVO content affirmed the reproducibility between the two methods. The repeatability of AMS results was tested on 10 aliquots of one of the blends, and the results were deemed reproducible.

The AMS Golden Valley laboratory is equipped with two accelerator mass spectrometers: the AMS facility from the Budker Institute of Nuclear Physics (BINP) and the Mini Carbon Dating System (MICADAS-28) from Ionplus AG and two graphitization systems: the Automated Graphitization Equipment (AGE-3) from Ionplus AG and the Absorption-catalytic setup (ACS) developed at the Boreskov Institute of Catalysis (BIC). The ACS was designed for graphite preparation from labeled biomedical samples, dissolved organics, and dissolved or gaseous carbon dioxide but has proven to be suitable for the traditional dating of objects no older than 35,000 years. Here we present two series of AMS data for the samples from Glasgow International Radiocarbon Inter-comparison (GIRI), prepared using AGE-3 and ACS, and then measured on MICADAS-28. The mean value of the background F14C was 0.0024 ± 0.0009 and 0.012 ± 0.003 for AGE-3 and ACS, respectively, and both methods gave reproducible results for the OXI.

Cathodes with recessed sample surfaces have several benefits in cesium sputter ion sources, including higher output, more efficient use of sample material, and improved focusing of the extracted ion beam. However, the Ionplus MICADAS uses cathodes with a graphite surface that is essentially flush with the sample holder. To evaluate the performance of recessed graphite with the MICADAS and determine the optimal surface depth, we tested four different depths, including the standard (flush) pressing method, 0.5 mm, 1.0 mm, and 1.5 mm. We found that recessed depths of 1.0 and 1.5 mm resulted in 20% higher ion beam current compared to the standard method under the same source conditions. The results are consistent with the beam produced from the recessed targets being more narrowly focused with a lower emittance, resulting in better transmission through the accelerator. Small graphite samples (200 µg C) with recessed surfaces produced higher currents for longer, leading to a 2–3× increase in sample ionization efficiency. Additionally, there was some evidence that isotopic ratio measurements of recessed samples were more stable over time. Overall, samples recessed to 1 mm depth offered numerous advantages over the standard pressing method and we have subsequently started pressing all MICADAS graphite using this approach.

Accelerator mass spectrometry radiocarbon (AMS 14C) dating was used for determining the age of wedge ice. It has been found that between 11,270 and 6420 cal BP, or the Greenlandian and Northgrippian stages of the Holocene, ice wedges grew syngenetically in sandy deposits with gravel in the Chara River valley. The variations of δ18O values in the ice wedges are about 8‰, from –25.5‰ to –18.8‰. Based on the stable isotope composition of ice wedges, paleotemperature reconstructions revealed that the mean January temperature was as low as –38°C during the coldest periods of the early half of the Holocene and as high as –28°C during the warmer periods.

The Eastern Chukotka is considered a unique permafrost region where massive ice bodies are widespread. However, the origin and age of these ice formations are often discussed. The age of the massive ice of Chukotka was established for the first time using AMS 14C dating. It was revealed that three massive ice bodies on the coast of Mechigmen Bay were formed at the end of the Late Pleistocene: a) near the Akkani site, 21,612 to 22,147 cal BP; b) near the Lavrentiya settlement, 27,553 cal BP; and c) near the Lavrentiya settlement, 22,193 cal BP. Stable isotope values in the studied massive ice vary in a rather wide range by about 10‰ for δ18O values (from –14.8‰ to –24.5‰) and about 75‰ for the δ2H values (from –116‰ to –191‰). The studied massive ice bodies are of intrasedimental genesis and formed epigenetically during the final stage of MIS2 (22–27 cal ka BP).

Radiocarbon (14C) methodology was used to investigate the presence of biocarbon in different bio-based disposable packaging products. Packaging waste contributes to a municipal solid waste, which is increasing environmental concerns and resulting in the enhancement of EU regulations that aim to reduce packaging waste. The 14C amount in samples reflects how much of the biocarbon has been used. In this study, the concentration of 14C was determined in commonly used types of disposable packaging, such as cups, plates, straws, cutlery, and baking paper. Samples were made of materials such as paper, wheat bran, sugarcane, and wood. The mean concentration of the 14C isotope, measured by the accelerator mass spectrometry (AMS) technique, is greater than 100 pMC in all tested samples, indicating that the samples are modern. The relatively high 14C concentration values in the waterproof layer of the sample indicate that bioplastic, rather than plastic, was used in its production. The highest 14C isotope concentration values were measured for samples that used the oldest biomass (wood and paper), and the lowest for products from current crops (sugarcane and wheat bran), which is consistent with the trend of changes in 14C concentration in the biosphere. The study also addresses the problem of heterogeneity and representativeness of subsamples.