Knotroot foxtail is a troublesome perennial grass found in pastures across the southeastern United States. Herbicides such as hexazinone and quinclorac are labeled for control of this weed, but their efficacy can be inconsistent due to delayed or excessive rain, which limits herbicide movement into the soil for root uptake, allowing knotroot foxtail rhizomes to survive and produce new shoots, resulting in reduced control. A greenhouse study was conducted in Alabama, in 2023 and 2024, to evaluate the effect of simulated rain timing on the efficacy of quinclorac and hexazinone in controlling knotroot foxtail. Knotroot foxtail plants averaging 28 cm tall were treated with quinclorac (0.4 kg ae ha−1) or hexazinone (0.8 kg ai ha−1), followed by simulated rain (6.3 mm) applied at 0, 3, 6, 9, 12, and 15 d after herbicide treatment. Hexazinone provided greater knotroot foxtail control and rhizome biomass reduction than quinclorac. At 51 d after each rain treatment (DAERT), hexazinone provided 90% control compared with 76% control with quinclorac. In 2024, at 51 DAERT, control with hexazinone ranged from 99% to 92% when rain occurred within 0 to 6 d after application, but declined to 85% and 81% when rain was delayed until 12 and 15 d, respectively. Similarly, knotroot foxtail was controlled by 87% to 77% when rain occurred within 0 to 9 d after quinclorac treatment, but control dropped to 67% to 62% when simulated rain was delayed until 12 to 15 d after the herbicide was applied. Relative to nontreated control plants, knotroot foxtail rhizome biomass was reduced by 72% and 42% after applications of hexazinone and quinclorac, respectively. An early rain (0 to 6 d) after herbicide application enhanced knotroot foxtail control, whereas delayed rain reduced herbicide effectiveness. This study underscores the importance of the timing of application when hexazinone or quinclorac is used for knotroot foxtail management before precipitation events.