Book contents
- Frontmatter
- Contents
- Preface
- 1 Introduction and data types
- 2 Using Canoco 5
- 3 Experimental design
- 4 Basics of gradient analysis
- 5 Permutation tests and variation partitioning
- 6 Similarity measures and distance-based methods
- 7 Classification methods
- 8 Regression methods
- 9 Interpreting community composition with functional traits
- 10 Advanced use of ordination
- 11 Visualising multivariate data
- 12 Case study 1: Variation in forest bird assemblages
- 13 Case study 2: Search for community composition patterns and their environmental correlates: vegetation of spring meadows
- 14 Case study 3: Separating the effects of explanatory variables
- 15 Case study 4: Evaluation of experiments in randomised complete blocks
- 16 Case study 5: Analysis of repeated observations of species composition from a factorial experiment
- 17 Case study 6: Hierarchical analysis of crayfish community variation
- 18 Case study 7: Analysis of taxonomic data with discriminant analysis and distance-based ordination
- 19 Case study 8: Separating effects of space and environment on oribatid community with PCNM
- 20 Case study 9: Performing linear regression with redundancy analysis
- Appendix A Glossary
- Appendix B Sample data sets and projects
- Appendix C Access to Canoco and overview of other software
- Appendix D Working with R
- References
- Index to useful tasks in Canoco 5
- Subject index
3 - Experimental design
Published online by Cambridge University Press: 05 May 2014
- Frontmatter
- Contents
- Preface
- 1 Introduction and data types
- 2 Using Canoco 5
- 3 Experimental design
- 4 Basics of gradient analysis
- 5 Permutation tests and variation partitioning
- 6 Similarity measures and distance-based methods
- 7 Classification methods
- 8 Regression methods
- 9 Interpreting community composition with functional traits
- 10 Advanced use of ordination
- 11 Visualising multivariate data
- 12 Case study 1: Variation in forest bird assemblages
- 13 Case study 2: Search for community composition patterns and their environmental correlates: vegetation of spring meadows
- 14 Case study 3: Separating the effects of explanatory variables
- 15 Case study 4: Evaluation of experiments in randomised complete blocks
- 16 Case study 5: Analysis of repeated observations of species composition from a factorial experiment
- 17 Case study 6: Hierarchical analysis of crayfish community variation
- 18 Case study 7: Analysis of taxonomic data with discriminant analysis and distance-based ordination
- 19 Case study 8: Separating effects of space and environment on oribatid community with PCNM
- 20 Case study 9: Performing linear regression with redundancy analysis
- Appendix A Glossary
- Appendix B Sample data sets and projects
- Appendix C Access to Canoco and overview of other software
- Appendix D Working with R
- References
- Index to useful tasks in Canoco 5
- Subject index
Summary
Some researchers still believe that multivariate methods are restricted to the exploration of data and to the generation of new hypotheses, but this has not been the case for several decades. In particular, constrained ordination is a powerful tool for analysing data from manipulative experiments. In this chapter, we review the basic types of experimental design, with an emphasis on manipulative field experiments.
Generally, we expect that the aim of an experiment is to compare the response of studied objects (e.g. an ecological community) to several treatments (treatment levels). One of the treatment levels is usually a control treatment, although in real ecological studies, it might be often difficult to decide what is the control (for example, when we compare several types of grassland management, which of the management types is the control one?). Detailed treatment of the topics handled in this chapter can be found for example in Underwood (1997).
If the response is univariate (e.g. number of species, total biomass), then the most common analytical tools are ANOVA, general linear models (which include both ANOVA, linear regression and their combinations), or generalized linear models. Generalized linear models are an extension of general linear models for the cases where the distribution of the response variable cannot be approximated by the normal distribution. These types of statistical models are further discussed in Chapter 8.
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- Information
- Multivariate Analysis of Ecological Data using CANOCO 5 , pp. 39 - 49Publisher: Cambridge University PressPrint publication year: 2014
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