Numerous exposures of highly altered kimberlites in exploratory trenches and pits have afforded an opportunity of studying the very great changes which take place chemically and mineralogically under conditions of tropical weathering in Sierra Leone.

The soils examined are from alluvial sheet deposits in a high valley where they are subjected to alternate wetting and drying, leading to desiccation. They are principally derived from shale and limestone bedrock in the surrounding mountains which are rapidly eroded and transported down valley mainly when the snow melts. The soils have engineering properties of fairly low density, high permeability, high plasticity with the plasticity index above the A line (CH range). These properties are correlated with the size grading and the clay mineralogical composition – about 30% calcite, 45-50 % clay minerals, dominated by Fe3+ and Mg-smectites and fine-grained muscovite. The clay mineralogy is explained in terms of Millot's zone of smectitization affecting biotite from detrital shale fragments, the muscovite remaining substantially unaltered. Calcite solution and redeposition account for the chalky texture and strong bond.

Background: To determine whether exosomal microRNAs (miRNAs) in CSF of patients with FTD can serve as diagnostic biomarkers, we assessed miRNA expression in the Genetic FTD Initiative (GENFI) cohort and in sporadic FTD. Methods: GENFI participants were either carriers of a pathogenic mutation or at risk of carrying a mutation because a first-degree relative was a symptomatic mutation carrier. Exosomes were isolated from CSF of 23 -pre-symptomatic and 15 symptomatic mutation carriers, and 11 healthy non-mutation carriers. Expression of miRNAs was measured using qPCR arrays. MiRNAs differentially expressed in symptomatic compared to pre-symptomatic mutation carriers were evaluated in 17 patients with sporadic FTD, 13 patients with sporadic Alzheimer’s disease (AD), and 10 healthy controls (HCs). Results: In the GENFI cohort, miR-204-5p and miR-632 were significantly decreased in symptomatic compared to pre-symptomatic mutation carriers. Decrease of miR-204-5p and miR-632 revealed receiver operator characteristics with an area of 0.89 [90% CI: 0.79-0.98] and 0.81 [90% CI: 0.68-0.93], and when combined an area of 0.93 [90% CI: 0.87-0.99]. In sporadic FTD, only miR-632 was significantly decreased compared to sporadic AD and HCs. Decrease of miR-632 revealed an area of 0.89 [90% CI: 0.80-0.98]. Conclusions: Exosomal miR-204-5p and miR-632 have potential as diagnostic biomarkers for genetic FTD and miR-632 also for sporadic FTD.

Introduction: Point-of-care ultrasound (POCUS) has been suggested as an initial investigation in the management of renal colic. Our objectives were: 1) to determine the accuracy of POCUS for the diagnosis of nephrolithiasis, and 2) to assess its prognostic value in the management of renal colic (PROSPERO: 42016035331). Methods: An electronic database search of MEDLINE, EMBASE, and PubMed was conducted utilizing subject headings, keywords, and synonyms that address our research question. Bibliographies of included studies and narrative reviews were manually examined. Studies of adult emergency department patients with renal colic symptoms were included. Any degree of hydronephrosis was considered a positive POCUS finding. Accepted criterion standards were CT evidence of renal stone or hydronephrosis, direct stone visualization, or surgical findings. Screening of abstracts, quality assessment with the QUADAS-2 instrument, and data extraction were performed by two reviewers, with discrepancies resolved by conference with a third reviewer.Test performance was assessed by pooled sensitivity and specificity, calculated likelihood ratios, and a summary receiver operator curve (SROC). The secondary outcome of prognostic value was reported as a narrative summary. Results: The electronic search yielded 627 unique titles. After relevance screening, 25 papers underwent full-text review, and 8 articles met all inclusion criteria. Of these, 5 high-quality studies (N=1773) were included in the meta-analysis for diagnostic accuracy, and three yielded data on prognostic value. The pooled results for sensitivity and specificity were 70.2% (95% CI=67.1% to 73.2%) and 75.4% (95% CI=72.5% to 78.2%), respectively. The calculated positive and negative likelihood ratios were 2.85 and 0.39. The SROC generated did not show evidence of a threshold effect.Three studies examining prognostic value noted a higher likelihood of a large stone or surgical intervention with positive POCUS findings. The largest randomized trial showed lower cumulative radiation exposure and no increase in adverse events in those who received POCUS investigation as the initial renal colic investigation. Conclusion: Point-of-care ultrasound is of modest accuracy for the diagnosis of nephrolithiasis. While positive POCUS findings are associated with larger stones and greater likelihood for intervention, the clinical importance of this is unclear.

Epidemiology formed the basis of ‘the Barker hypothesis’, the concept of ‘developmental programming’ and today’s discipline of the Developmental Origins of Health and Disease (DOHaD). Animal experimentation provided proof of the underlying concepts, and continues to generate knowledge of underlying mechanisms. Interventions in humans, based on DOHaD principles, will be informed by experiments in animals. As knowledge in this discipline has accumulated, from studies of humans and other animals, the complexity of interactions between genome, environment and epigenetics, has been revealed. The vast nature of programming stimuli and breadth of effects is becoming known. As a result of our accumulating knowledge we now appreciate the impact of many variables that contribute to programmed outcomes. To guide further animal research in this field, the Australia and New Zealand DOHaD society (ANZ DOHaD) Animals Models of DOHaD Research Working Group convened at the 2nd Annual ANZ DOHaD Congress in Melbourne, Australia in April 2015. This review summarizes the contributions of animal research to the understanding of DOHaD, and makes recommendations for the design and conduct of animal experiments to maximize relevance, reproducibility and translation of knowledge into improving health and well-being.

We have successfully demonstrated optical aperture synthesis at the 4-m Anglo-Australian Telescope. By using a multi-hole mask over the (re-imaged) primary mirror and recording the resulting fringe patterns with high time resolution, diffraction-limited images of sufficiently bright objects can be reconstructed. The data processing uses closure phases to overcome the effects of atmospheric turbulence. We show an image of the double star η Oph, with component separation 0″.45.

Several outbreaks of hepatitis A in men who have sex with men (MSM) were reported in the 1980s and 1990s in Australia and other countries. An effective hepatitis A virus (HAV) vaccine has been available in Australia since 1994 and is recommended for high-risk groups including MSM. No outbreaks of hepatitis A in Australian MSM have been reported since 1996. In this study, we aimed to estimate HAV transmissibility in MSM populations in order to inform targets for vaccine coverage in such populations. We used mathematical models of HAV transmission in a MSM population to estimate the basic reproduction number (R 0) and the probability of an HAV epidemic occurring as a function of the immune proportion. We estimated a plausible range for R 0 of 1·71–3·67 for HAV in MSM and that sustained epidemics cannot occur once the proportion immune to HAV is greater than ~70%. To our knowledge this is the first estimate of R 0 and the critical population immunity threshold for HAV transmission in MSM. As HAV is no longer endemic in Australia or in most other developed countries, vaccination is the only means of maintaining population immunity >70%. Our findings provide impetus to promote HAV vaccination in high-risk groups such as MSM.

A single nucleotide polymorphism rs12807809 located upstream of the neurogranin (NRGN) gene has been identified as a risk variant for schizophrenia in recent genome-wide association studies. To date, there has been little investigation of the endophenotypic consequences of this variant, and our own investigations have suggested that the effects of this gene are not apparent at the level of cognitive function in patients or controls. Because the impact of risk variants may be more apparent at the level of brain, the aim of this investigation was to delineate whether NRGN genotype predicted variability in brain structure and/or function. Healthy individuals participated in structural (N = 140) and/or functional (N = 36) magnetic resonance imaging (s/fMRI). Voxel-based morphometry was used to compare gray and white matter volumes between carriers of the non-risk C allele (i.e., CC/CT) and those who were homozygous for the risk T allele. Functional imaging data were acquired during the performance of a spatial working memory task, and were also analyzed with respect to the difference between C carriers and T homozygotes. There was no effect of the NRGN variant rs12807809 on behavioral performance or brain structure. However, there was a main effect of genotype on brain activity during performance of the working memory task, such that while C carriers exhibited a load-independent decrease in left superior frontal gyrus/BA10, TT individuals failed to show a similar decrease in activity. The failure to disengage this ventromedial prefrontal region, despite preserved performance, may be indicative of a reduction in processing efficiency in healthy TT carriers. Although it remains to be established whether this holds true in larger samples and in patient cohorts, if valid, this suggests a potential mechanism by which NRGN variability might contribute to schizophrenia risk.

An analysis was undertaken to measure age-specific vaccine effectiveness (VE) of 2010/11 trivalent seasonal influenza vaccine (TIV) and monovalent 2009 pandemic influenza vaccine (PIV) administered in 2009/2010. The test-negative case-control study design was employed based on patients consulting primary care. Overall TIV effectiveness, adjusted for age and month, against confirmed influenza A(H1N1)pdm 2009 infection was 56% (95% CI 42–66); age-specific adjusted VE was 87% (95% CI 45–97) in <5-year-olds and 84% (95% CI 27–97) in 5- to 14-year-olds. Adjusted VE for PIV was only 28% (95% CI −6 to 51) overall and 72% (95% CI 15–91) in <5-year-olds. For confirmed influenza B infection, TIV effectiveness was 57% (95% CI 42–68) and in 5- to 14-year-olds 75% (95% CI 32–91). TIV provided moderate protection against the main circulating strains in 2010/2011, with higher protection in children. PIV administered during the previous season provided residual protection after 1 year, particularly in the <5 years age group.

Recently, multiple-locus variable-number tandem-repeat analysis (MLVA) has been proposed as an alternative to pulsed-field gel electrophoresis (PFGE) for characterization of Escherichia coli O157:H7. In this study we characterized 118 E. coli O157:H7 isolates from cases of gastrointestinal disease in New Zealand using XbaI PFGE profiles and a MLVA scheme that assessed variability in eight polymorphic loci. The 118 isolates characterized included all 80 E. coli O157:H7 referred to New Zealand's Enteric Reference Laboratory in 2006 and 29 phage-type 2 isolates from 2005. When applied to these isolates the discriminatory power of PFGE and MLVA was not significantly different. However, MLVA data may be more epidemiologically relevant as isolates from family clusters of disease had identical MLVA profiles, even when the XbaI PFGE profiles differed slightly. Furthermore, most isolates with indistinguishable XbaI PFGE profiles that did not appear to be epidemiologically related had distinct MLVA profiles.

European farms at Abercorn had been suffering from bovine trypanosomiasis carried by Glossina morsitans Westw., and the Tanganyika Department of Tsetse Research were asked in 1935 to advise.

Fly-pickets were established in 1936 to prevent carriage of flies on to the farms, and a belt of country was protected from fire, with the object of thickening the woody vegetation so as to make it unfavourable to G. morsitans. The fire-protected area was extended and continued until 1946; serious escape fires occurred in 1938 and 1941. During these first five years the reduction in catches at the pickets was only about one-third.

An intensive survey of the area was made in 1941, as the result of which concentrations of flies were identified in certain shallow valleys forming the headwaters of small tributary streams. Several of these were cleared of trees, with the result that the apparent density of G. morsitans was at once depressed not only in the valleys themselves but over wide areas of untouched woodland round about.

In 1942 and the following years these clearings were extended to new valley sites as they were discovered, both inside and outside the fire-excluded area, and always with striking effects. The last clearing of all was done in 1952.

Six individual tectonostratigraphic units are identified within the İzmir–Ankara–Erzincan Suture Zone in the critical Erzincan area of the Eastern Pontides. The Ayıkayası Formation of Campanian–Maastrichtian age is composed of bedded pelagic limestones intercalated with polymict, massive conglomerates. The Ayıkayası Formation conformably overlies the Tauride passive margin sequence in the Munzur Mountains to the south and is interpreted as an underfilled foredeep basin. The Refahiye Complex, of possible Late Cretaceous age, is a partial ophiolite composed of ~75% (by volume) serpentinized peridotite (mainly harzburgite), ~20% diabase and minor amounts of gabbro and plagiogranite. The complex is interpreted as oceanic lithosphere that formed by spreading above a subduction zone. Unusual screens of metamorphic rocks (e.g. marble and schist) locally occur between sheeted diabase dykes. The Upper Cretaceous Karayaprak Mélange exhibits two lithological associations: (1) the basalt + radiolarite + serpentinite association, including depleted arc-type basalts; (2) the massive neritic limestone + lava + volcaniclastic association that includes fractionated, intermediate-composition lavas, and is interpreted as accreted Neotethyan seamount(s). The several-kilometre-thick Karadağ Formation, of Campanian–Maastrichtian age, is composed of greenschist-facies volcanogenic rocks of mainly basaltic to andesitic composition, and is interpreted as an emplaced Upper Cretaceous volcanic arc. The Campanian–Early Eocene Sütpınar Formation (~1500 m thick) is a coarsening-upward succession of turbiditic calcarenite, sandstone, laminated mudrock, volcaniclastic sedimentary rocks that includes rare andesitic lava, and is interpreted as a regressive forearc basin. The Late Paleocene–Eocene Sipikör Formation is a laterally varied succession of shallow-marine carbonate and siliciclastic lithofacies that overlies deformed Upper Cretaceous units with an angular unconformity. Structural study indicates that the assembled accretionary prism, supra-subduction zone-type oceanic lithosphere and volcanic arc units were emplaced northwards onto the Eurasian margin and also southwards onto the Tauride (Gondwana-related) margin during Campanian–Maastrichtian time. Further, mainly southward thrusting took place during the Eocene in this area, related to final closure of Tethys. Our preferred tectonic model involves northward subduction, supra-subduction zone ophiolite genesis and arc magmatism near the northerly, Eurasian margin of the Mesozoic Tethys.

A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigotelike forms in Schneider's Drosophila medium supplemented with 2% (v/v) foetal calf serum. Unlike previous methods, it utilizes direct culture of parasites obtained from the lesions of infected animals rather than adaptation of promastigotes in vitro. Ultrastructural (possession of megasomes), biochemical (cysteine proteinase activity and gelatin SDS-PAGE banding pattern) and infectivity (in vivo) data are presented which show the close similarity of the cultured forms to lesion amastigotes. The axenically cultured forms grew optimally at a temperature of 32–33 °C, providing further evidence for their amastigote nature. It was found that adjustment of the pH of the growth medium to 5·4 was required in order to retain the amastigote morphology of the cultured parasites. This supports the notion that leishmanial amastigotes are acidophiles.

1.Within a flock of 55 Welsh Mountain ewes maintained under lowland conditions the first ewecame into oestrus on the 14th October. The mean cycle length of 44 normal cycles was 16 days 3 hr.

2. The onset of oestrus as assessed by the time of mounting of the ram does not appear to beevenly distributed throughout the day (24 hr.).