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The Black-cheeked Lovebird Agapornis nigrigenis has a highly restricted range in dry south-western Zambia, where its distribution is clumped and localised in association with mopane Colophospermum mopane woodland and permanent water pools. Fieldwork and monitoring over 30 months between December 2018 and October 2021 established that the lovebirds’ usage of pools for drinking was higher towards the centre of the bird’s distribution and influenced by the pools’ proximity to mopane woodlands, surrounding tree cover, and level of human activity. Of the four pool types available for use by lovebirds (i.e. mopane, grassland, river, and artificial), mopane and grassland pools were disproportionally susceptible to drying out in the dry season, hence showed greater variation in numbers of visiting birds compared with the other two types. Lovebirds showed a preference for pools with a perimeter of <50 m and tended to avoid those with a perimeter >100 m, consistent with a positive association between pool size and human activity. Convergence between humans and lovebirds in dependence on water resources and mopane woodland points to the need to find ways to overcome potential conflicts. Such ways include creating small, shallow-sided, undisturbed pools in or near mopane woodland, extending water retention in existing mopane pools, and enhancing the capacity of artificial pools to meet the needs of the lovebirds.
The Bali myna Leucopsar rothschildi has long suffered heavy trapping, leading to its near extinction in the wild and categorization as Critically Endangered on the IUCN Red List. Decades of conservation breeding, release of birds and post-release management at Bali Barat National Park have, until recently, failed to secure a viable wild population. However, over the past decade, population increases, expansion into new areas of the National Park and beyond, and successful breeding in both artificial and natural nest sites have occurred. These recent successes are associated with a change in approach by the National Park authority from concentrating efforts on the last refugium of the species (an area protected from trapping but with potentially suboptimal habitat) and towards the human-dominated landscapes around the main road through the National Park. Bali mynas tended to favour areas with extensive shorter grass cover and open canopies and to shun denser woodland. Anthropogenic landscapes such as farmland and plantations presumably mimic the original savannah habitat of the species, but nestbox provision has probably been crucial in these areas in the absence of natural cavities. A potential further factor in the increases in myna numbers and range has been a scheme involving local people in commercial breeding of the species, thereby reducing its market price, and working with communities to reduce trapping pressure. We encourage continuing operation of this management strategy inside the National Park and its further extension into adjacent tourist areas, which appear to have myna-friendly socio-ecological conditions.
There is serious concern for the future of a wide range of birds in Java and elsewhere in Indonesia due to both loss of habitat and trapping for the cagebird trade (the so-called “Asian Songbird Crisis”). Despite this concern, few data on presence and abundance of key species exist. We provide such data on 184 bird species from over two years of biodiversity surveys from 37 sites on 12 mountains in West and Central Java. Many of these species are heavily traded, endemic, and globally threatened. Several of the threatened endemics, notably Javan Trogon and Javan Cochoa, were often recorded, in terms of both geographical spread and numerical abundance. Rufous-fronted Laughingthrush, Spotted Crocias, and Orange-spotted Bulbul, believed to be threatened by trapping for the songbird trade, appear to remain fairly widespread. By contrast, Brown-cheeked Bulbul, Chestnut-backed (Javan) Scimitar-babbler, Javan Oriole, and especially Javan Blue-flycatcher, recorded on just a single occasion, and Javan Green Magpie, which we failed to record with certainty, now appear to be extremely rare. Our encounter rates, while not pinned to specific mountains for security reasons, represent an important baseline against which future changes in abundance can be gauged.
Effectiveness of buprenorphine (BUP) is moderated by medication misuse diversion and non-adherence, and poor retention and high cost. Contingency Managment has added benefit to BUP with Take-home doses as the most preferred reward by Opioid Use Disorder patients.
Objectives
Examine the clinical effectiveness of a novel incentivised medication adherence and abstinence monitoring to enable contingent access to increasing BUP take-home doses.Explore associations with opioid use and retention. Contrast characteristics of polysubstance abusers (PSA) and response to BUP, with single opioid users.
Methods
Two-arm, pragmatic, 16-week outpatient RCT of BUP maintenance.Takehome doses were provided as stepped-approach upto 4-weeks contingent of abstinence (UDS) and adherence according to Therapeutic Drug Monitoring-TDM. Primary outcome and secondard outcomes were % negative UDS for opioids anx retention, respectively. -
Results
Opioid % negative UDS was 76.7% (SD 25.0%) in I-AAM versus 63.5% (SD 34.7%) in TAU (13.3%; 95% [CI] 3.2%–23.3%; Cohen’s d 0.44; 95% CI 0.10–0.87). In I-AAM, 40 participants (57.1%) were retained versus 33 (46.4%) in TAU [OR: 1.54; 95% CI 0.79–2.98). PSA (73.7%, n=104) and carisprodol use increases non-fatal overdose (OR) 3.83, 95% CI 1.25 to 11.71) and 5.31, 95% CI 1.92 to 14.65], respectively. Opioid and non-opioid UDS are positively associated. BUP elimination rate (BUP-EL.R) predicts 26.5% to 65% of negative opioid UDS [Beta - 89.95, 95% CIl -154.20 to -25.70, R2 0.22]. Family enagement increases retention by 3-fold.
Conclusions
BUP + incentivised TDM for contingent access to increasing take-home doses increased abstinence. BUP-EL.R seems promising in BUP treatment precision and BUP is clinically valuable in polysubstance abusers.Engaging family enhances retention.
The extent to which findings in bilingualism research are contingent on specific analytic choices, experimental designs, or operationalisations, is currently unknown. Poor availability of data, analysis code, and materials has hindered the development of cumulative lines of research. In this review, we survey current practices and advocate a credibility revolution in bilingualism research through the adoption of minimum standards of transparency. Full disclosure of data and code is necessary not only to assess the reproducibility of original findings, but also to test the robustness of these findings to different analytic specifications. Similarly, full provision of experimental materials and protocols underpins assessment of both the replicability of original findings, as well as their generalisability to different contexts and samples. We illustrate the review with examples where good practice has advanced the agenda in bilingualism research and highlight resources to help researchers get started.
Designating protected areas remains a core strategy in biodiversity conservation. Despite high endemism, montane forests across the island of Java are under-represented in Indonesia's protected area network. Here, we document the montane biodiversity of Gunung Slamet, an isolated volcano in Central Java, and provide evidence to support its increased protection. During September–December 2018, we surveyed multiple sites for birds, primates, terrestrial mammals, reptiles, amphibians and vegetation. Survey methods included transects, camera traps and targeted searches at six sites, at altitudes of 970–2,512 m. We used species distribution models for birds and mammals of conservation concern to identify priority areas for protection. We recorded 99 bird species (13 globally threatened), 15 mammals (five globally threatened) and 17 reptiles and amphibians (two endemic). Our species distribution models showed considerable cross-taxon congruence between important areas on Slamet's upper slopes, generally above 1,800 m. Particularly important were records of the endemic subspecies of the Endangered Javan laughingthrush Garrulax rufifrons slamatensis, not recorded in the wild since 1925, the Endangered Javan gibbon Hylobates moloch and Javan surili Presbytis comata, and the Vulnerable Javan lutung Trachypithecus auratus and Javan leopard Panthera pardus melas. Recent forest loss has been modest, at least 280 km2 of continuous forest remain above 800 m, and our surveys show that forest habitats are in good condition. However, the mountain is widely used by trappers and hunters. Given its importance for biodiversity conservation, we discuss different options for improving the protection status of Gunung Slamet, including designation as a National Park or Essential Ecosystem.
While populations of the Endangered Grey Parrot Psittacus erithacus have collapsed across its range, the species remains remarkably abundant on the island of Príncipe, Gulf of Guinea. We examine how aspects of its ecology interplay with local environmental conditions, to inform conservation strategies for this species and other large parrots. On Príncipe, parrots breed in large trees of common species, with nest densities (42 ± 34 km−2) greatly exceeding those for any comparably sized parrot. Productivity is high (1.9 chicks per cavity), probably reflecting the absence of nest competitors and predators. Food sources are abundant and much of the island is inaccessible to trappers, so many nests are successful each year. Historically harvest has involved taking only chicks from trees in a few traditional patches. These conditions have combined to allow Grey Parrots to thrive on Príncipe, while elsewhere nest trees are timber targets, nest competition and nest predation are likely to be more intense, trapping is indiscriminate, and few areas remain unexploited by trappers. Preservation of large trees as breeding refugia, and vigilance against the indiscriminate trapping of adult birds, are identified as key conditions to stabilize and recover mainland Grey Parrot populations and indeed large parrots generally, given their very similar ecological traits and anthropogenic circumstances.
Liben Lark Heteromirafra archeri is a ‘Critically Endangered’ species threatened by the loss and degradation of grassland at the Liben Plain, southern Ethiopia, one of only two known sites for the species. We use field data from nine visits between 2007 and 2019 and satellite imagery to quantify changes over time in the species’ abundance and in the extent and quality of its habitat. We estimate that the population fell from around 279 singing males (95% CL: 182–436) in 2007 to around 51 (14–144) in 2013, after which too few birds were recorded to estimate population size. Arable cultivation first appeared on the plain in the early 1990s and by 2019 more than a third of the plain had been converted to crops. Cultivation was initially confined to the fertile black soils but from 2008 began to spread into the less fertile red soils that cover most of the plain. Liben Larks strongly avoided areas with extensive bare ground or trees and bushes, but the extent of these did not change significantly over the survey period. A plausible explanation for the species’ decline is that grassland degradation, caused before 2007 by continuous high-pressure grazing by livestock, reduced its rates of reproduction or survival to a level that could not support its previous population. Since 2015, communal kalos (grazing exclosures) have been established to generate forage and other resources in the hope of also providing breeding habitat for Liben Larks. Grass height and density within four grassland kalos in 2018 greatly exceeded that in the surrounding grassland, indicating that the plain retains the potential to recover rapidly if appropriately managed. Improvement of grassland structure through the restitution of traditional and sustainable rangeland management regimes and the reversion of cereal agriculture to grassland are urgently needed to avert the species’ extinction.
The aim of this work is to report on the tumour control probability (TCP) of a UK cohort of lung stereotactic ablative radiotherapy patients (n = 198) for a range of dose and fractionations common in the UK.
Materials and methods:
TCP values for 3 (54 Gy), 5 (55 and 60 Gy) and 8 (50 Gy) fraction (#) schemes were calculated with the linear-quadratic Marsden TCP model using the Biosuite software.
Results:
TCP values of 100% were computed for the 3 # and for 5 # (α/β = 10 Gy) cohorts; reduced to 99% (range 97–100) for the 5 # cohort only when an α/β of 20 Gy was used. The average TCP value for the 50 Gy in 8 # regime was 97% (range 92–99, α/β = 10 Gy) and 64% (range 48–79, α/β = 20 Gy). Statistical significant differences were observed between the α/β of 10 Gy versus 20 Gy groups and between all data grouped by fraction.
Conclusion:
TCPs achievable with current planning techniques in the UK have been presented. The ultra-conservative 50 Gy in 8 # scheme returns a significantly lower TCP than the other regimes.
Although both the grey parrot Psittacus erithacus and the recently recognized timneh parrot Psittacus timneh are categorized as Endangered because of harvest for the pet trade and loss of habitat, the latter has a much smaller range and may be largely restricted to a few stronghold areas. In March–April 2018 we surveyed for a total of 114 hours in and around one of these presumed strongholds, the large and well-protected Gola Rainforest National Park, the Sierra Leonean portion of the Gola Transboundary Peace Park. Timneh parrots were encountered at a rate of 0.1 groups/h in the National Park and 0.3 in the buffer zone, indicating densities of 1–3 individuals per km2. These figures are similar to recent density estimates from the Liberian side of the Peace Park, suggesting that the transboundary population amounts to c. 2,400 individuals inside the Park and an unknown number in the surrounding areas. Densities of the timneh parrot may be generally low even in strongholds, its numbers may be declining steeply, and the global population size is probably lower than previously believed.
Introduction: September 2017 saw the launch of the British Columbia (BC) Emergency Medicine Network (EM Network), an innovative clinical network established to improve emergency care across the province. The intent of the EM Network is to support the delivery of evidence-informed, patient-centered care in all 108 Emergency Departments and Diagnostic & Treatment Centres in BC. After one year, the Network undertook a formative evaluation to guide its growth. Our objective is to describe the evaluation approach and early findings. Methods: The EM Network was evaluated on three levels: member demographics, online engagement and member perceptions of value and progress. For member demographics and online engagement, data were captured from member registration information on the Network's website, Google Analytics and Twitter Analytics. Membership feedback was sought through an online survey using a social network analysis tool, PARTNER (Program to Analyze, Record, and Track Networks to Enhance Relationships), and semi-structured individual interviews. This framework was developed based on literature recommendations in collaboration with Network members, including patient representatives. Results: There are currently 622 EM Network members from an eligible denominator of approximately 1400 physicians (44%). Seventy-three percent of the Emergency Departments and Diagnostic and Treatment Centres in BC currently have Network members, and since launch, the EM Network website has been accessed by 11,154 unique IP addresses. Online discussion forum use is low but growing, and Twitter following is high. There are currently 550 Twitter followers and an average of 27 ‘mentions’ of the Network by Twitter users per month. Member feedback through the survey and individual interviews indicates that the Network is respected and credible, but many remain unaware of its purpose and offerings. Conclusion: Our findings underscore that early evaluation is useful to identify development needs, and for the Network this includes increasing awareness and online dialogue. However, our results must be interpreted cautiously in such a young Network, and thus, we intend to re-evaluate regularly. Specific action recommendations from this baseline evaluation include: increasing face-to-face visits of targeted communities; maintaining or accelerating communication strategies to increase engagement; and providing new techniques that encourage member contributions in order to grow and improve content.
Among the solar proxies, κ1 Cet, stands out as potentially having a mass very close to solar and a young age. We report magnetic field measurements and planetary habitability consequences around this star, a proxy of the young Sun when life arose on Earth. Magnetic strength was determined from spectropolarimetric observations and we reconstruct the large-scale surface magnetic field to derive the magnetic environment, stellar winds, and particle flux permeating the interplanetary medium around κ1 Cet. Our results show a closer magnetosphere and mass-loss rate 50 times larger than the current solar wind mass-loss rate when Life arose on Earth, resulting in a larger interaction via space weather disturbances between the stellar wind and a hypothetical young-Earth analogue, potentially affecting the habitability. Interaction of the wind from the young Sun with the planetary ancient magnetic field may have affected the young Earth and its life conditions.
In preparation for the wind tunnel testing of a series of aerodynamic research models a strain-gauged balance was sought which could be successfully made without the use of sophisticated tools. The principal requirements which influenced the design of the balance were:
(a) It must enable lift, drag and pitching moment to be measured.
(b) Deformation of the balance under the aerodynamic loads to be small.
(c) Use of only basic workshop tools.
(d) Short manufacturing time.
Further, the models were to be sting mounted at one end of the balance with their span vertical. The other end of the balance was to be rigidly fixed to a supporting stand which would be mounted on the wind tunnel turntable to permit incidence variation (Fig. 5).
Background: The pathophysiology of subarachnoid hemorrhage (SAH) is complex and includes disruption of the blood-brain barrier (BBB). We freshly isolated BBB endothelial cells (BECs) by 2 distinct methods after experimental SAH and then interrogated their gene expression profiles with the goal of uncovering new therapeutic targets. Methods: SAH was induced using the prechiasmatic blood injection mouse model. BBB permeability studies were performed by administering intraperitoneal cadaverine dye injections at 24h and 48h. BECs were isolated either by sequential magnetic-based sorting for CD45-CD31+ cells or by fluorescence-activated cell sorting (FACS) for Tie2+Pdgfrb- cells. Total RNA was extracted and analyzed using Affymetrix Mouse Gene 2.0 ST Arrays. Results: BBB impairment occurred at 24h and resolved by 48h after SAH. Analysis of gene expression patterns in BECs at 24h reveal clustering of SAH and sham samples. We identified 707 (2.8%) significant differentially-expressed genes (403 upregulated, 304 downregulated) out of 24,865 interrogated probe sets. Many significantly upregulated genes were involved in inflammatory pathways. These microarray results were validated with real-time polymerase chain reaction (RT-PCR). Conclusions: This study is the first to investigate in an unbiased manner, whole genome expression profiling of freshly-isolated BECs in an SAH animal model, yielding targets for novel therapeutic intervention.
Introduction: Atrial fibrillation and flutter (AFF) are the most common arrhythmias presenting to the emergency department. A coordinated ED AFF electronic order-set and management pathway was developed in collaboration with cardiologists at our institution. The primary objective of this study was to compare the ED length of stay pre and post pathway implementation. Secondary objectives included comparison of the following outcomes pre and post-pathway (PRE & POST): AFF Clinic referral rates, ED return rates, and mortality. Methods: This was a retrospective case series of patients presenting to our quarternary care ED with AFF pre and post AFF pathway implementation. Cases were identified using an administrative database covering 120 000 annual ED visits. Trained research assistants and the primary investigator extracted data from the electronic medical record. 20% of all charts were double collected to ensure accuracy (k=0.85). Descriptive variables were described using counts, means, medians and confidence intervals. Chi-square statistics of dependent samples were calculated for the primary outcome. Results: We examined 307 cases of AFF presenting to our ED (n=130 PRE; n=177 POST). Demographic variables were similar PRE and POST: mean age (66.0 [95%CI 63.8-68.3] PRE; 65.0 [63.0-67.0] POST), % male (59.2% PRE; 59.3% POST), presenting rhythm (66.2% A.fib [58.0-74.3] A. flutter 29.2% [21.4-37.0] PRE; 61.0% A.fib [53.8-68.1] A. flutter 17.5% [11.9-23.1] POST), and CHADS2VASC score (2.1 [1.8-2.4] PRE; 1.9 [1.7-2.1] POST). The mean ED LOS decreased by 72.5 minutes (95% CI -22.9 to -122.1; P < 0.001). AFF clinic referral rates increased from 16.9% PRE to 25.4% POST (not significant). ED return rates within 30 days for AFF, CHF, major bleeding and CVA were unchanged. 30 day mortality rates were not statistically different (1.5% PRE vs. 2.8% POST). Conclusion: A coordinated ED AFF pathway was associated with a significant reduction in ED LOS without significant changes in ED return rates or mortality.
Introduction: Vancouver Coastal Health (VCH) emergency physicians have been on contract based funding models for two decades. On October 1, 2015, physicians at one hospital (SPH) switched to fee-for-service (FFS) payments. Conventional wisdom is that FFS physicians are motivated to see more patients quickly and achieve higher throughput. Our hypothesis was that FFS payment would reduce patient wait times. Methods: This interrupted time series analysis with concurrent control was performed in VCH Region, where there are two tertiary EDs. During the 20-week study period (July 15-Nov 30), VGH remained on contract, while SPH converted to FFS (the intervention). VCH administrative data was aggregated by week. Our primary outcome was median wait time to MD. Secondary outcomes were ED LOS and left-without-being-seen (LWBS) rates. Results: Interrupted time series plots will be presented for the data. Data from 67,214 ED visits were analyzed (31,733 SPH, 35,481 VGH). Figure 1 shows that baseline wait time was 74 minutes at the control and 53 minutes at the intervention site. During the pre-intervention period, there was a non-significant downward trend of 0.4 minutes per week at the intervention hospital relative to control (p=0.26). After FFS conversion, there was a 4.1 minute increase in wait time at the control site (p=0.18), and a significant downward trend of 1.4 minutes per week (p=0.001). After FFS conversion, wait times at the intervention site increased by 4.8 minutes more than control (p-value for the difference=0.27), and the wait time trend increased significantly by 1.3 minutes per week relative to the expected counterfactual trend (p=0.02). Baseline EDLOS for discharged patients was 227 minutes at the control hospital and 193 minutes at the intervention site. There were similar pre-intervention LOS increases at both hospitals. Post-intervention, both sites saw significant increases in EDLOS, followed by a similar downward trends of -2.68 minutes per week (p=0.001). Baseline LWBS rate was 3.86% at the control hospital and 3.56% at the intervention site. Pre-intervention trends, and post-intervention level/trend changes did not differ by site. Conclusion: Conversion to FFS payment was associated with an increase in wait time trend of 1.3 minutes per week relative to control. There were no significant changes in EDLOS or LWBS rates. In this preliminary analysis, FFS payment had little effect on wait times or patient throughput.
Introduction: Understanding physician human resources in British Columbia’s (BC) emergency settings is essential to plan for training, recruitment and professional development programs. In 2014 we conducted an online and phone survey to the site leads for the 95 Emergency Departments (ED) attached to hospitals in BC. Methods: A one-page survey was developed by the authors (JC and JM). Each hospital listed on the BC Ministry of Health’s website was contacted to confirm that they had a functioning ED attached to the hospital and to determine who their site lead was. Each ED site lead was then emailed the questionnaire and up to three more follow-up emails and direct phone requests were performed as needed. Results: 92 of the 95 EDs completed the survey and we discovered that just over 1000 physicians deliver emergency care in BC with approximately half doing so in combination with family practice. There was an estimated shortfall of 199 physicians providing emergency care in 2014 and an anticipated shortfall of 287 by 2017 and 399 by 2019. Slightly more than half had formal certification, with 28% through the Royal College of Canada and 70% with the College of Family Physicians of Canada. Conclusion: More than 1000 physicians care for patients in EDs across BC but there is a significant and growing need for more physicians. There is tremendous variation across health authorities in emergency medicine certification, but approximately half of those who deliver emergency care have formal certification. Despite limitations of a survey method, this provides the most accurate and current estimate of emergency practitioner resources and training in BC and will be important in guiding discussions to address the identified gaps.
Introduction: Atrial fibrillation and flutter (AFF) are the most common arrhythmias presenting to the emergency department. Without anticoagulation, AFF increases stroke risk; individuals with paroxysmal AFF have a similar prognosis. A coordinated ED AFF electronic order-set and management pathway was developed at our institution. The primary objective of this study was to measure rates of appropriate anticoagulation (AAC) on discharge from the ED for patients presenting with AFF not previously on antithrombotic or anticoagulant medications. Secondary objectives included comparison of the following outcomes pre and post-pathway (PRE & POST): AFF Clinic referral rates, ED return rates, and mortality. Methods: This was a retrospective case series of patients presenting to our quarternary care ED with AFF pre and post AFF pathway implementation. Cases were identified using an administrative database covering 120 000 annual ED visits. Trained research assistants and the primary investigator extracted data from the electronic medical record. 20% of all charts were double collected to ensure accuracy (k=0.85). Descriptive variables were described using counts, means, medians and confidence intervals. Chi-square statistics of dependent samples were calculated for the primary outcome. Results: We examined 307 cases of AFF presenting to our ED (n=130 PRE; n=177 POST). Demographic variables were similar PRE and POST: mean age (66.0 [95%CI 63.8-68.3] PRE; 65.0 [63.0-67.0] POST), % male (59.2% PRE; 59.3% POST), presenting rhythm (66.2% A.fib [58.0-74.3] A. flutter 29.2% [21.4-37.0] PRE; 61.0% A.fib [53.8-68.1] A. flutter 17.5% [11.9-23.1] POST), and CHADS2VASC score (2.1 [1.8-2.4] PRE; 1.9 [1.7-2.1] POST). The rate of AAC rose from 39.1% PRE to 77.8% POST (P < 0.01). AFF clinic referral rates increased from 16.9% PRE to 25.4% POST (not significant). ED return rates within 30 days for AFF, CHF, major bleeding and CVA were unchanged. 30 day mortality rates were not statistically different (1.5% PRE vs. 2.8% POST). Conclusion: The implementation of a coordinated ED AFF pathway was associated with significant improvements in the proportion of patients discharged with appropriate anticoagulation who had not previously been on antithrombotic or anticoagulant medications. ED return rates and mortality did not change significantly.
Estimating population sizes in the heavily traded grey parrots of West and Central Africa would provide insights into conservation status and sustainability of harvests. Ideally, density estimates would be derived from a standardized method such as distance sampling, but survey efforts are hampered by the extensive ranges, patchy distribution, variable abundance, cryptic habits and high mobility of the parrots as well as by logistical difficulties and limited resources. We carried out line transect distance sampling alongside a simpler encounter rate method at 10 sites across five West and Central African countries. Density estimates were variable across sites, from 0–0.5 individuals km−2 in Côte d'Ivoire and central Democratic Republic of the Congo to c. 30 km−2 in Cameroon and > 70 km−2 on the island of Príncipe. Most significantly, we identified the relationship between densities estimated from distance sampling and simple encounter rates, which has important applications in monitoring grey parrots: (1) to convert records of parrot groups encountered in a day's activities by anti-poaching patrols within protected areas into indicative density estimates, (2) to confirm low density in areas where parrots are so rare that distance sampling is not feasible, and (3) to provide a link between anecdotal records and local density estimates. Encounter rates of less than one parrot group per day of walking are a reality in most forests within the species’ ranges. Densities in these areas are expected to be one individual km−2 or lower, and local harvest should be disallowed on this basis.