Radiocarbon (14C) dating of soil samples by accelerator mass spectrometry (AMS) has been proven useful for studying carbon (C) cycling in terrestrial ecosystems. However, this application has two primary difficulties in sample preparation: inhibition of graphite formation due to sulfur (S)-containing impurities and contamination of samples with modern C (MC). Herein, we evaluated these effects using three sample preparation methods (silver foil, silver wire, and Sulfix) by conducting AMS-14C measurements of a 14C-dead charred wood and S-rich soil samples. The preparation methods were all successful in graphite formation and AMS-14C measurement for soil samples with an organic S content <6.9 wt%. The methods showed different percent modern carbon (pMC) values from 0.16% to 0.64% for the 14C-dead sample. The results also revealed that across different methods, MC contamination can be significantly reduced by applying two-step procedure (combustion and subsequent reaction to remove S-containing impurities) during sample preparation. The three methods had a negligible influence on determining the 14C age for samples that were at least younger than 12,000 yr BP. As the 14C ages of the soil samples are typically younger than 12,000 yr BP, any method explored in this study can be employed for 14C dating with sufficient accuracy for application to C cycle studies.

Hybrid Langmuir-Blodgett (LB) films of a single clay layer and a donor-(π-electron system)-acceptor (Dδ-π-Aδ−) zwitterionic molecule (ind-TCNQ), prepared from 1,3,3-trimethyl-2-methyleneindoline (donor) and 7,7,8,8-tetracyanoquinodimethane (acceptor) were prepared. The characteristics of the electrostatic interaction between the clay layer and the ind-TCNQ molecules were investigated. The amount of ind-TCNQ molecules spread onto the air-water interface was increased systematically so that mono- and multi-layers of the molecules could be formed on the clay layer. The hybrid LB films, which were deposited onto a solid substrate, were characterized by means of UV-vis absorption spectroscopy and optical second-harmonic generation measurement. The amount of ind-TCNQ molecules adsorbed on the clay layer increased with an increase in the amount of ind-TCNQ molecules spread onto the air-water interface. On the other hand, the SHG intensities of the films did not change with the change in amount of ind-TCNQ spread. The results indicate that the electrostatic interaction between the clay layer and the ind-TCNQ molecules operates only in the region where the ind-TCNQ molecules are in direct contact with the clay layer.

Regional assessments of ice elevation change provide insight into the processes controlling an ice sheet's geometric response to climate forcing. In Southwest Greenland's land terminating sector (SWLTS), it is presumed that ice surface elevation changes result solely from changing surface mass balance (SMB). Here we test this assumption by developing a multi-decadal (1985–2017) record of elevation change from digital elevation models (DEMs) and comparing it to regional climate model output and available records of ice speed. The SWLTS thinned by >12 m on average over the full 32-year period, but the change was highly variable in time and space. Thinning was amplified in the central region of the SWLTS, relative to the north and south. During 1985–2007, the north and south regions demonstrated net thickening while the central region thinned. Regional differences in elevation change are inconsistent with SMB anomalies, indicating that enhanced ice flow in the north and south contributed to thickening during this early time interval. While clear validation in the south is prevented by incomplete velocity data, historical surface speeds in the north were elevated. These findings support the interpretation that changing ice flow can influence ice surface elevation in the slow-moving SWLTS.

On the Kamchatka Peninsula in the Russian Far East, 405 glaciers with an estimated total mass of 49 Gt were reported in the 1970s. These have been retreating at an accelerated rate since the start of the 21st century. Because glacier studies in this region are scarce, ice loss and its influence on sea level rise and regional environments is poorly understood. In this study, we analyzed satellite data to quantify glacier mass change from 2000 to 2016 in six major glacier-covered regions on the peninsula. The mean rate of the glacier mass change over the study period was −0.46 ± 0.01 m w.e. a−1 (total mass change was −4.9 ± 0.1 Gt, −304.2 ± 9.1 Mt a−1), which is slightly lower than other regions in mid-latitude and subarctic zones. The mass loss accelerated from >−0.33 ± 0.02 m w.e. a−1 in the period 2000–2006/2010 to <−1.65 ± 0.12 m w.e. a−1 in 2006/2010–2015/16. The increase in mass loss is attributed to a rise in average decadal summer temperatures observed in the region (+0.68°C from 1987–99 to 2000–13). Moreover, a recent trend in Pacific decadal oscillation suggests future acceleration of mass loss due to a decline in winter precipitation.

Diabetes mellitus is a metabolic disease spreading worldwide that has been reported to worsen the development and progression of other diseases (cancer, vascular diseases and dementia). To establish functional rice lines with anti-postprandial hyperglycaemic effects, we developed mutant rice lines, which lack one or two gene(s) related to starch synthesis, and evaluated their effects. Powder of mutant rice lines or other grains was loaded to rats fasted overnight (oral grain powder loading test). Incremental area under time-concentration curves (iAUC) were calculated with monitored blood glucose levels. Rice lines with anti-postprandial hyperglycaemic effects were separated by cluster analysis with calculated iAUC. A double mutant rice #4019 (starch synthase IIIa (ss3a)/branching enzyme IIb (be2b)), one of the screened mutant rice lines, was fed to Goto-Kakizaki (GK) rats, an animal model for type 2 diabetes, for 5 weeks. Plasma levels of C-peptide, a marker of pancreatic insulin secretion, were measured with ELISA. For in vitro study, a rat pancreatic cell line was cultured with a medium containing rat serum which was sampled from rats fed #4019 diet for 2 d. After 24-h of incubation, an insulin secretion test was performed. Through the oral rice powder loading test, seven rice lines were identified as antidiabetic rice lines. The intake of #4019 diet increased plasma C-peptide levels of GK rats. This result was also observed in vitro. In rat serum added to cell medium, ornithine was significantly increased by the intake of #4019. In conclusion, the mutant rice #4019 promoted pancreatic insulin secretion via elevation of serum ornithine levels.

By examining party-switching decisions among members of Japan's Liberal Democratic Party (LDP), this article shows how distributive policy programs exclusively available to the governing party attract incumbents to the party in power. In a stable electoral environment where the government party is likely to stay in power, legislators elected from infrastructure-poor constituencies are effectively tied to the party. However, when the party's electoral prospects are uncertain, legislators behave more sincerely and switch parties to match their policy preferences. It is also found that defectors elected from infrastructure-poor constituencies tended to return to the LDP once the party installed a stable surplus coalition.

We explore in this article an institutional foundation of agricultural protectionism in Japan, a country long recognized as resisting international pressures to open up its rice market. Using our qualitative analysis of postwar politics of agricultural protectionism and a simple formal model, we argue that farmers in Japan have stronger incentives to mobilize electoral support for the governing party in multimember district systems than in single-member district systems, because the marginal effects of mobilization on policy benefits are different under these electoral systems. Our empirical findings corroborate this claim and provide implications for the gradual changes in Japan's farm policies occurring after the electoral reform in 1994.

In this article we examine the role of local politicians in affecting national-level election outcomes by focusing on the drastic municipal mergers in Japan that took place in the early 2000s. Specifically, we argue that the political party that relies most extensively on local politicians' efforts for electoral mobilization and monitoring will suffer an electoral slump when municipalities are merged and the number of municipal politicians is swiftly reduced. We empirically show that municipalities with a history of mergers exhibit significantly lower voter turnout and obtain a smaller vote share for the ruling Liberal Democratic Party in national elections when compared to other municipalities without an experience of mergers. This result indicates that municipal politicians are indispensable human resources for LDP candidates running for the national parliament.

Brown adipose tissue (BAT) is responsible for cold- and diet-induced thermogenesis, and thereby contributes to the control of whole-body energy expenditure (EE) and body fat content. BAT activity can be assessed by fluoro-2-deoxyglucose (FDG)-positron emission tomography (PET) in human subjects. Grains of paradise (GP, Aframomum melegueta), a species of the ginger family, contain pungent, aromatic ketones such as 6-paradol, 6-gingerol and 6-shogaol. An alcohol extract of GP seeds and 6-paradol are known to activate BAT thermogenesis in small rodents. The present study aimed to examine the effects of the GP extract on whole-body EE and to analyse its relation to BAT activity in men. A total of nineteen healthy male volunteers aged 20–32 years underwent FDG-PET after 2 h of exposure to cold at 19°C with light clothing. A total of twelve subjects showed marked FDG uptake into the adipose tissue of the supraclavicular and paraspinal regions (BAT positive). The remaining seven showed no detectable uptake (BAT negative). Within 4 weeks after the FDG-PET examination, whole-body EE was measured at 27°C before and after oral ingestion of GP extract (40 mg) in a single-blind, randomised, placebo-controlled, crossover design. The resting EE of the BAT-positive group did not differ from that of the BAT-negative group. After GP extract ingestion, the EE of the BAT-positive group increased within 2 h to a significantly greater (P< 0·01) level than that of the BAT-negative group. Placebo ingestion produced no significant change in EE. These results suggest that oral ingestion of GP extract increases whole-body EE through the activation of BAT in human subjects.

This study was performed to determine the rates of breastfeeding among singletons, twins and triplets in Japan, and identify factors associated with the decision to breastfeed or bottle-feed. We analyzed a database of medical check-up of infants aged 3 to 6 months between April 2001 and July 2004 in Nishinomiya City in Japan. This medical check-up is given to almost 100% of infants in Japan and the data of 15,262 infants were analyzed. Among these, 14,963 (98.0%) were singletons, 290 (1.9%) were twins and 9 (0.1%) were triplets. Exclusive breast-feeding was chosen by 6680 (43.8%) mothers, mixed-feeding by 4645 (30.4%) mothers, and bottle-feeding with formula milk only by 3900 (25.6%) mothers. The rate of exclusive breastfeeding among twins or triplets was significantly lower than among singleton babies: 4.1% among twins or triplets, and 44.7% among singletons. Moreover, twins and triplets were independently associated with a higher rate of bottle-feeding: the odds ratio indicated that mothers who had twins or triplets were 2.44 times more likely to choose bottle-feeding with formula milk only than those who had singletons. Sucking ability at birth was associated with a higher rate of bottle-feeding: the odds ratio indicated that mothers who had infants with poor sucking ability at birth were 1.56 times more likely to choose bottle-feeding as those who had infants with normal sucking ability.

This study was performed using population-based data to analyze whether motor development in early life is different between singletons and twins in Japan. For better comparison and investigation, we divided the 2 groups into a group with a birthweight of 2.5 kg or greater (subgroup A) and a group with a birthweight of less than 2.5 kg (subgroup B), respectively. We analyzed the database of medical check-ups for children aged 3 years between April 2001 and July 2004. They received medical checkups at 4 months, 1.5, and 3 years of age. Children who were suspected of having neurological abnormality or disability were referred to specialists and excluded from the database. The data of 14,132 children were analyzed. Among these, 13,040 (92.3%) children were singletons in subgroup A, 75 (0.5%) were twins in subgroup A, 866 (6.1%) were singletons in subgroup B and 151 (1.1%) were twins in subgroup B. The mean age at achieving milestones was slower in twins of subgroup A for each developmental outcome than singletons of subgroup A, and the difference between twins and singletons was significant after adjustment for rolling over. On the contrary, after adjusting for a confounding factor (gestational age), singletons of subgroup B attained motor development facilitating walking independently slower than twins of subgroup B. There were different tendencies in the results regarding the motor development of subjects of subgroup A and that of subjects of subgroup B.

In this paper, we consider pairs of forbidden subgraphs that imply the existence of a 2-factor in a graph. For d ≥ 2, let d be the set of connected graphs of minimum degree at least d. Let F1 and F2 be connected graphs and let be a set of connected graphs. Then {F1, F2} is said to be a forbidden pair for if every {F1, F2}-free graph in of sufficiently large order has a 2-factor. Faudree, Faudree and Ryjáček have characterized all the forbidden pairs for the set of 2-connected graphs. We first characterize the forbidden pairs for 2, which is a larger set than the set of 2-connected graphs, and observe a sharp difference between the characterized pairs and those obtained by Faudree, Faudree and Ryjáček. We then consider the forbidden pairs for connected graphs of large minimum degree. We prove that if {F1, F2} is a forbidden pair for d, then either F1 or F2 is a star of order at most d + 2. Ota and Tokuda have proved that every -free graph of minimum degree at least d has a 2-factor. These results imply that for k ≥ d + 2, no connected graphs F except for stars of order at most d + 2 make {K1,k, F} a forbidden pair for d, while for every connected graph F makes {K1,k, F} a forbidden pair for d. We consider the remaining range of , and prove that only a finite number of connected graphs F make {K1,k, F} a forbidden pair for d.

The oxygen reduction reaction (ORR) mechanism of non-Pt catalysts was elucidated by investigating electronic structures of carbon alloy catalysts (CACs) for polymer electrolyte membrane fuel cells (PEMFC). For metal phthalocyanine-based carbon alloy catalysts with 1-2% of nitrogen and less than 0.1% of Co or Fe, photoelectron spectroscopy, X-ray absorption spectroscopy (XAS), and X-ray Absorption Fine Structure (XAFS) have revealed that Co or Fe metal sites are not the ORR active site, whereas the carbon atoms adjacent to graphitic nitrogens at a zigzag edge of graphite may act as the ORR active site, which is in good agreement with first principles calculations. These studies have also revealed that the zigzag edges, detected as a XAS shoulder component, are well evolved for catalysts pyrolyzed at 600°C, which show the maximum ORR activity. Based on these analyses, we have realized significantly improved carbon alloy based ORR performance, up to about 70 % of that with Pt catalysts. Further, to analyze the electronic structure of CACs during operation, we constructed a new in situ soft X-ray emission spectroscopy system with very high energy resolution (ΔE) of 120 meV at 640 eV at the University of Tokyo beamline BL07LSU in SPring-8.

The phonon distribution of hydrogen storage α-LaNi5H with 4h, 6m, 12n, and 12o interstitial hydrogen was calculated by using first-principles potential surfaces with a 2×2×2 supercell model in order to investigate structural and thermodynamic properties. Frequency shifts due to the phonon contribution from the internal energies of 12n < 6m < 12o < 4h appeared in specific modes originating from interstitial hydrogen and in the upper-edge modes with nickel-lattice motion. The thermodynamic stability of 12n interstitial hydrogen in α-LaNi5H due to the wide XZ storage space can be explained by its phonon amplitudes and the charge density around nickel-bonded hydrogen.

Lower barriers of entry for new firms and more flexibility in structuring a business organisation are the two key factors motivating the introduction of the new company law. In general, policymakers use new company law initiatives to encourage entrepreneurship, innovation and cooperative arrangements. This paper distinguishes the diverse strands of company law reforms arising in the United States, Europe and Asia and points to the underlying conditions that shape the markedly different reform outputs. Our analysis points to three important factors – (1) private ordering; (2) fiscal transparency; and (3) limited liability – that effect the incentives for new firm creation. However, we find that many of the new company law reforms are incomplete. Nevertheless, these new company law reforms retain the ability to generate rents due to their adaptability and responsiveness to social and economic change.