The literature in political science considers (sometimes inaccurate) perceptions of immigrants as a factor in anti-immigration attitudes among natives, but much less is known about perceptions regarding immigrants from specific regions. In this paper, I explore Americans’ perceptions about immigrants from Africa, Asia, Europe, Latin America, and the Middle East. To measure these perceptions, I apply a conjoint experiment with a multinomial outcome, in which respondents are asked to categorize hypothetical immigrants as coming from one of the five regions. Results from a nationally diverse sample demonstrate that immigrants from all regions other than Europe are associated with speaking poor English. Immigrants from Latin America are also associated with welfare dependency and rule-breaking behavior, while the opposite is true for immigrants from Asia. These negative perceptions may at least partly explain opposition to non-European, and specifically Latin American, immigration in the United States.