During vocabulary instruction, it is important to teach words until their representations are robust enough to be retained. For adults, the number of training sessions a target item is successfully retrieved during training predicts the likelihood of post-training retention. To assess this relationship in children, we reanalyzed data from Gordon et al. (2021b, 2022). Four- to six-year-old children completed six training days with word form-object pairs and were tested one month later. Results indicate that the number of training sessions that a word form was retrieved was positively related to post-training retention. We discuss implications for vocabulary instruction and interventions.

A clinical decision tree was developed using point-of-care characteristics to identify patients with culture-proven sepsis due to extended-spectrum β-lactamase–producing Enterobacterales (ESBL-PE). We compared its performance with the clinical gestalt of emergency department (ED) clinicians and hospital-based clinicians. The developed tree outperformed ED-based clinicians but was comparable to inpatient-based clinicians.

Subcutaneous adipose tissue (scAT) and peripheral blood mononuclear cells (PBMC) play a significant role in obesity-associated systemic low-grade inflammation. High-fat diet (HFD) is known to induce inflammatory changes in both scAT and PBMC. However, the time course of the effect of HFD on these systems is still unknown. The aim of the present study was to determine the time course of the effect of HFD on PBMC and scAT. New Zealand white rabbits were fed HFD for 5 or 10 weeks (i.e. HFD-5 and HFD-10) or regular chow (i.e. control (CNT)-5 and CNT-10). Thereafter, metabolic and inflammatory parameters of PBMC and scAT were quantified. HFD induced hyperfattyacidaemia in HFD-5 and HFD-10 groups, with the development of insulin resistance in HFD-10, while no changes were observed in scAT lipid metabolism and inflammatory status. HFD activated the inflammatory pathways in PBMC of HFD-5 group and induced modified autophagy in that of HFD-10. The rate of fat oxidation in PBMC was directly associated with the expression of inflammatory markers and tended to inversely associate with autophagosome formation markers in PBMC. HFD affected systemic substrate metabolism, and the metabolic, inflammatory and autophagy pathways in PBMC in the absence of metabolic and inflammatory changes in scAT. Dietary approaches or interventions to avert HFD-induced changes in PBMC could be essential to prevent metabolic and inflammatory complications of obesity and promote healthier living.

Infectivity of third-stage larvae of Brugia malayi was assessed following intraperitoneal inoculation into jirds, Meriones unguiculatus. Larvae were of two ages and were derived from two sites in Aedes aegypti mosquitoes, i.e., specimens collected from the thorax 11 days after infection and from the head on day 14. Larvae from the thorax had just completed the second moult and measured 990 to 1100μm in length. Only 6% of these specimens developed to adult worms in jirds. Larvae that migrated to the head were 1400 to 1700 μm long on day 14 and, in contrast, 23% of the inocula developed to adult worms. This study establishes that all third-stage larvae, regardless of their age or location in the arthropod host, are potentially infective. However, pronounced physical maturation does seem to be accompanied by a marked increase in infectivity.

Laboratory and field-cage studies were conducted in Texas in 1965 to evaluate treatments of the systemic insecticides, Azodrin (3-hydroxy-N-methyl-cis-crotonamide dimethyl phosphate), Bidrin (3-hydroxy-N, N dimethyl-cis-crotonamide dimethyl phosphate), American Cyanamid CL-47031 (cyclic ethylene (diethoxy-phosphinyl) dithioimidocarbonate) and Temik (2-methyl-2-(methylthio) propion-aldehyde O-(methylcarbamoyl) oxime), applied incorporated in lanolin to the stems of cotton plants against Heliothis zea (Boddie) and H. virescens (F.). Reductions in numbers of developing larvae of H. zea were substantial on individual plants the stems of which had been treated with Azodrin or CL-47031 and which were artificially infested with eggs. When first-instar larvae of H. zea or H. virescens were caged on plants 3, 7 or 14 days after stem treatment with 2.5, 5.0 or 100 mg. Azodrin, Bidrin or CL-47031 per plant, net mortalities ranged from 21 to 80 per cent after three days. The mortality of adults of H. zea provided with sucrose solutions containing 1 p.p.m. of the systemic insecticides indicated that Azodrin and Bidrin were about equally toxic and much more so than CL-47031 and Temik, and that of adults caged on individual plants in flower that had been treated with Azodrin or CL-47031 suggested that the moths may be killed by the systemic action of these insecticides translocated to the nectar. When adults of H. virescens were released on plants each treated with Azodrin at 25 or 30 mg. in large field cages, reductions in the numbers of eggs deposited, attributed to the effect on the moths of the insecticide in the nectar, and in the numbers of developing larvae, were substantial. Azodrin was the most consistently effective of the four insecticides evaluated.

The metabolism of the amino acid l-tryptophan is a highly regulated physiological process leading to the generation of several neuroactive compounds within the central nervous system. These include the aminergic neurotransmitter serotonin (5-hydroxytryptamine, 5-HT), products of the kynurenine pathway of tryptophan metabolism (including 3-hydroxykynurenine, 3-hydroxyanthranilic acid, quinolinic acid and kynurenic acid), the neurohormone melatonin, several neuroactive kynuramine metabolites of melatonin, and the trace amine tryptamine. The integral role of central serotonergic systems in the modulation of physiology and behaviour has been well documented since the first description of serotonergic neurons in the brain some 40 years ago. However, while the significance of the peripheral kynurenine pathway of tryptophan metabolism has also been recognised for several decades, it has only recently been appreciated that the synthesis of kynurenines within the central nervous system has important consequences for physiology and behaviour. Altered kynurenine metabolism has been implicated in the pathophysiology of conditions such as acquired immunodeficiency syndrome (AIDS)-related dementia, Huntington's disease and Alzheimer's disease. In this review we discuss the molecular mechanisms involved in regulating the metabolism of tryptophan and consider the medical implications associated with dysregulation of both serotonergic and kynurenine pathways of tryptophan metabolism.

We have previously shown greatly enhanced resistance to stressinduced hillock formation through fluorine incorporation in aluminum films. Utilizing relatively low F incorporation (<0.1 atomic %), hillock formation density is reduced ∼10x over pure or similarly Cu-doped aluminum films. Electromigration tests were performed on a matrix of structures with varying topology (step heights and slopes) and fluorine incorporation dose. We find that although F improves the stress-induced hillock formation by an order of magnitude, the electromigration performance of flat structures is only slightly improved with F incorporation. Analyzing various step heights and step slopes, the nonfluorinated Al experienced a decreasing electromigration lifetime with increasing step height. However, the optimally implanted F samples showed almost no lifetime reduction with step coverages over a similar regime. In addition, scanning electron micrographs of the failed samples revealed that the failures of the fluorinated samples differ markedly from the non-fluorinated samples. Finally, SIMS profiles taken on F and Cu (for comparison) implanted samples reveal the fundamentally different nature of the two beneficial components: Cu redistributes relatively easily throughout the Al film to segregate to grain boundaries. In contrast, the F profile is extremely stable with simnilar anneals and provides its beneficial effect by forming a distributed refractory metal-like structure within the interconnect.

There has been some uncertainty as to the impact of fluorine (F) on SiO2 quality and reliability. Several laboratories have shown greatly enhanced quality and reliability with fluorinated oxides, while others have been unable to repeat the results. In addition, the laboratories which have shown enhanced reliability with the fluorinated oxides have differed in their interpretation of the mechanism by which the enhancement occurs. X-ray diffraction stress measurements, partial time dependent dielectric breakdown (TDDB) measurements, SIMS depth profiling, transmission electron microscopy, standard high/low frequency C-V measurements, and hot-carrier aging of variously processed MOSFETs have been used to investigate a variety of fluorinated films. We believe that the apparent lack of consistency of the effects of fluorine on MOSFET reliability between laboratories may be explained by slight variations in the gate polysilicon processing which result in variations in polysilicon morphology. The polysilicon morphology determines both mechanical stress and F diffusion which ultimately impacts interface state density and thus hot carrier reliability.

The National Enforcement Investigations Center of the EPA provides support services for the enforcement activities of the Agency. Recently, we have analyzed hazardous wastes as part of efforts to enforce the Resource Conservation and Recovery Act and the Superfund Act. Sample preparation for inorganic elemental analysis is a difficult and time-consuming step. Thus, it would be desirable to be able to use x-ray fluorescence methods which require relatively little sample preparation for the analysis of solid hazardous wastes. A major problem to be overcome is the need to calibrate for a large variety of samples. However, a compensating factor is that the error will be largely determined by the sampling error and the measurement accuracy is not quite so critical.