The ability to detect and monitor errors enables us to maintain optimal performance across tasks. One neurophysiological index of error monitoring is the error-related negativity (ERN), a fronto-central negative deflection peaking between 0 and 150 ms following an erroneous response. While the developmental literature has illustrated age-related differences in the ERN and its association with anxiety, the literature has mainly focused on the between-person differences of the ERN. Our study examined the within-person variations of the ERN in 115 community-dwelling 9- to 12-year-olds (66 girls; mean age/SD = 11.00/1.16 years). Participants completed an EEG Go/No-Go task and reported their anxiety symptoms. Multilevel growth analyses yielded significant within-person, curvilinear changes in the ERN throughout the task. Youths’ trial-level ERN increased (i.e., became more negative) with early errors, but decreased with subsequent errors. This curvilinear pattern was evident in older, but not younger, youths. Age also interacted with anxiety symptoms: younger youths with higher anxiety showed a continuous increase in the ERN throughout the task, whereas older youths with higher anxiety showed an initial increase followed by a decline in the ERN. Our study contributed novel evidence for the development of the ERN and the underlying mechanisms of the ERN-anxiety relationship that cannot be captured by between-person approaches.

The housing condition of pig (Sus scrofa) fattening farms are increasingly receiving criticism, because they are associated with impaired animal welfare. Consumers view the increase in farm sizes critically, even though scientifically based knowledge on the relationship between farm size and welfare is still limited. Therefore, the aim of this study was to assess the welfare level of conventional fattening pig farms in Germany and to evaluate the relationship between farm size and animal welfare level. In total, the Welfare Quality® protocol (WQ) for pigs was applied on 60 farms. Farms were classified according to their size into small (< 1,500 pigs per farm), medium (1,500-3,000 pigs per farm) and large (> 3,000 pigs per farm). Independent of the farm size, the overall WQ classifications ‘excellent’ and ‘not classified’ were not recorded in any of the farms, while ‘enhanced’ and ‘acceptable’ was achieved by 80 and 20% of the farms, respectively. Farm sizes had no effect on any of the four principles ‘good feeding’, ‘good housing’, ‘good health’ or ‘appropriate behaviour’. Overall, moderate bursitis (35%) was found to be the most prevalent indicator of welfare-related problems. However, it did not differ between farm sizes. Another highly prevalent indicator, moderately soiled body, increased from 11.1% in small-to 20.8% in large-sized farms. In conclusion, our findings show that none of the farm sizes were superior in terms of animal welfare. Overall, acceptable or enhanced scores were achieved for many of the criteria, however the need for improvement in other criteria such as ‘expression of other behaviour’ and ‘positive emotional state’, was clear.

OBJECTIVES/GOALS: The use of Human Centered Design (HCD) to improve the quality of team science is a recent application, and HCDs benefits and challenges have not been rigorously evaluated. We conducted a qualitative study with health sciences researchers trained in HCD methods to determine how they applied HCD methods and perceived its benefits and challenges. METHODS/STUDY POPULATION: The University of Pittsburgh offered HCD training to three cohorts of research scientists (staff as well as faculty) over a three-year period. The training was provided by the LUMA Institute, a premier HCD design firm with a highly regarded training program. We then evaluated this training by conducting 1-hour, semi-structured interviews with trainees from three training cohorts. Interviews focused on perceptions of the training, subsequent uses of HCD, barriers and facilitators, and perceptions of the utility of HCD to science teams. Data analysis was conducted using Braun and Clarkes process for thematic analysis. RESULTS/ANTICIPATED RESULTS: We interviewed 18 researchers (nine faculty and nine staff) trained in HCD methods and identified distinct themes regarding HCD use and its perceived benefits and challenges. Trainees found HCD relevant to research teams for stakeholder engagement, research design, project planning, meeting facilitation, and team management. They also described benefits of HCD in five distinct areas: creativity, egalitarianism, structure, efficiency, and visibility. We also identified challenges, including tensions between HCD approaches and academic culture. DISCUSSION/SIGNIFICANCE: Our data suggest that HCD has the potential to help researchers work more inclusively and collaboratively on interdisciplinary teams and generate more innovative and impactful science. The application of HCD methods is not without challenges; however, we believe these challenges can be overcome with institutional investment.

The Área de Conservación Osa (ACOSA) contains the largest population of Scarlet Macaws Ara macao in Costa Rica. Despite their influence on ecosystem dynamics and status as a flagship species, empirical data on the foraging patterns of this population is lacking. This information is crucial in implementing effective conservation strategies, particularly reintroduction attempts. Observations of feeding behaviour were made systematically over a 12-month period to provide the first direct examination of Scarlet Macaw diet within the ACOSA region. Scarlet Macaws feed on various items including seeds, flowers, bark, and leaf-gall larvae. Key findings included a demonstration of a smaller dietary niche breadth than that recorded for other Central American populations, use of button mangrove Conocarpus erectus, a species not previously recognised as a food source for Scarlet Macaws, and a heavy reliance on an exotic non-native species, Terminalia catappa. We argue that whilst human-modified coastal locations may present viable habitat for Scarlet Macaws, anthropogenic influences including the removal of native food sources and proliferation of exotic and cultivated species have left the Scarlet Macaws of the ACOSA particularly dependent on a small number of species.

Issues in traditional cross-section sampling of paintings and other cultural artifacts with a scalpel, such as crumbling, delamination and paint compression, can deter conservators from sampling fragile paint layers. Often, such sampling carries the risk of causing further damage from a scalpel, which outweighs the benefits of scientific investigation. Here, we show that femtosecond lasers offer a viable alternative to obtaining cross-sections with minimal damage to the surrounding artwork. A Regenerative Ti:Sapphire amplifier system with a pulse duration of 70 femtoseconds, a few milliwatts of average power and a repetition rate of 1 kHz (1000 pulses/sec) was used for the study. Tests were performed on oil paintings ranging in age from the 19th century to late 20th century. Effective settings were determined to be 2 mW of power at a speed of 10mm/sec using an 800nm laser. Preliminary results suggest femtosecond lasers could be a viable alternative for obtaining paint cross-sections when traditional sampling methods cause unnecessary damage to fragile materials.

By reducing the number of dimensions that light can propagate in from three down to two, one may gain control over the characteristics of propagation. This control can allow for “Stopped Light” (SL), where wavepackets of light are slowed down to a zero group velocity. This is achieved by designing planar metal-dielectric structures that are stacked in one dimension allowing for waveguide modes in the other two, and engineering the dispersion relation of these structures. Stopped light structures can be further optimized to reduce their dispersion and increase the number of spatial frequencies supported, which allows for confinement of electromagnetic energy over volumes smaller than the diffraction limit over fixed regions in space. If this electromagnetic energy is confined over a region that provides gain, the question arises, can amplification of this light energy occur? and indeed can a regime of lasing be entered into? We show that stopped light lasing is indeed possible, despite there being no resonant cavity in 2d to confine the light, and explore the properties of this new type of laser.

Production systems for fattening pigs have been characterized over the last 2 decades by rising farm sizes coupled with increasing group sizes. These developments resulted in a serious public discussion regarding animal welfare and health in these intensive production systems. Even though large farm and group sizes came under severe criticism, it is still unknown whether these factors indeed negatively affect animal welfare. Therefore, the aim of this study was to assess the effect of group size (<15 v. 15 to 30 v. >30 pigs/pen) on various animal-based measures of the Welfare Quality® protocol for growing pigs under conventional fattening conditions. A total of 60 conventional pig fattening farms with different group sizes in Germany were included. Moderate bursitis (35%) was found as the most prevalent indicator of welfare-related problems, while its prevalence increased with age during the fattening period. However, differences between group sizes were not detected (P>0.05). The prevalence of moderately soiled bodies increased from 9.7% at the start to 14.2% at the end of the fattening period, whereas large pens showed a higher prevalence (15.8%) than small pens (10.4%; P<0.05). With increasing group size, the incidence of moderate wounds with 8.5% and 11.3% in small- and medium-sized pens, respectively, was lower (P<0.05) than in large-sized ones (16.3%). Contrary to bursitis and dirtiness, its prevalence decreased during the fattening period. Moderate manure was less often found in pigs fed by a dry feeder than in those fed by a liquid feeding system (P<0.05). The human–animal relationship was improved in large in comparison to small groups. On the contrary, negative social behaviour was found more often in large groups. Exploration of enrichment material decreased with increasing live weight. Given that all animals were tail-docked, tail biting was observed at a very low rate of 1.9%. In conclusion, the results indicate that BW and feeding system are determining factors for the welfare status, while group size was not proved to affect the welfare level under the studied conditions of pig fattening.

Farm Service Agency (FSA) direct loans are intended to provide transitory credit to creditworthy borrowers unable to obtain conventional credit at reasonable terms. Farm loan program (FLP) effectiveness is measured in part by how readily direct loan borrowers graduate to conventional credit. A survey of FSA borrowers originating direct loans during fiscal years 1994-1996 is used to estimate graduation rates. A majority of 1994-1996 loan originators did exit the direct FLP by November 2004. A multinomial logit model indicates financial strength at origination resulted in greater likelihood of farming without direct loans approximately 9 years after loan origination.

The Victorian poet Gerard Manley Hopkins already demonstrated a special sensitivity to nature as a young Anglican. But his conversion to Catholicism, followed by his formation as a Jesuit, nurtured a creation spirituality that moved him from the rather cold view of the cosmos typical of his Victorian era to a vibrant sense of God intimately revealed in nature. This new sense of being a creature involved in an intimate personal relationship with the Creator comes from Hopkins' appropriation of the creation spirituality of Ignatius of Loyola. After reviewing the evolution of worldviews from the medieval synthesis melded with the Newtonian mechanical model (the Victorian picture) to our contemporary cosmic “story,” this article then samples poems that illustrate the creation spirituality that Hopkins absorbed from Ignatius' vision. This vision is remarkably in tune with the new sense of the place of the human creature in the cosmic story that the sciences now tell regarding the emergence of matter, life, and persons.

In August 2008, a large outbreak of Shiga toxin-producing Escherichia coli (STEC) O111:NM infections associated with a buffet-style restaurant in rural Oklahoma was identified. A case-control study of restaurant patrons and a retrospective cohort study of catered event attendees were conducted coupled with an environmental investigation to determine the outbreak's source and mode of transmission. Of 1823 persons interviewed, 341 (18·7%) met the outbreak case definition; 70 (20·5%) were hospitalized, 25 (7·3%) developed haemolytic uraemic syndrome, and one died. Multiple food items were significantly associated with illness by both bivariate and multivariate analyses, but none stood out as a predominant transmission vehicle. All water, food, and restaurant surface swabs, and stool cultures from nine ill employees were negative for the presence of Shiga toxin and E. coli O111:NM although epidemiological evidence suggested the outbreak resulted from cross-contamination of restaurant food from food preparation equipment or surfaces, or from an unidentified infected food handler.

Biomolecules rich in aspartic acid (Asp) are known to play a role inbiomineral morphology and polymorph selection, and have been shown togreatly enhance the growth kinetics of calcite. The mechanism by which thesecompounds favor calcification may be related to their effects upon cationsolvation. Using molecular dynamics, we investigated the influence of smallcarboxylated molecules on the hydration states and water exchange rates ofdivalent cations. We show that the carboxylate moieties of Asp promotedehydration of Ca2+ and Sr2+ and that contact ion pair(CIP) formation is not required to disrupt the hydration of these cations. Ca2+- Asp and Sr2+ - Asp CIP formation decreasesthe total inner sphere coordination from an average of 8.0 and 8.4 in bulkwater to 7.5 and 8.0, respectively. Water residence times estimated for Mg2+, Ca2+and Sr2+ follow the expectedtrend of decreasing residence time with increasing ionic radius. In thepresence of Asp, both solvent-separated ion pair (SSIP) and CIP formationdecrease the residence times of Ca2+and Sr2+ innersphere water molecules. Comparable impacts on Mg2+ hydration arenot observed. Mg2+ - Asp CIP formation is energeticallyunfavorable and Asp does not affect Mg2+ inner sphere waterresidence times.

Studies have shown that in the mammalian neuromuscular system stretch reflexes are localized within individual muscles. Neuromuscular compartmentalization, the partitioning of sensory output from muscles, and the partitioning of segmental pathways to motor nuclei have also been demonstrated. This evidence indicates that individual motor nuclei and the muscles they innervate are not homogeneous functional units. An analysis of the functional significance of reflex localization and partitioning suggests that segmental control mechanisms are based on subdivisions of motor nuclei–muscle complexes. A partitioned organization of segmental control mechanisms could utilize (1) the potential functional diversity of muscle fiber types, (2) the variety of mechanical actions of individual muscles arising from their distributed origins and insertions, and (3) diverse architectural features such as intramuscular variations in pinnation and complex in-series and in-parallel arrangements of muscle fibers. The differentiated activity observed in some muscles during natural movements also calls for localized segmental control mechanisms. Partitioning may also play a role in mechanical interactions between contracting motor units and in increasing the stability of neuromuscular systems. The functional advantages of reflex localization and partitioning suggest they are probably common features of segmental systems, whose organization reflects the structure and function of their associated neuromuscular systems.

A recent study showed physicians' reasoning about a realistic case to be ignorant of base rate. It also showed physicians interpreting information pertinent to base rate differently, depending on whether it was presented early or late in the case. Although these adult reasoners might do better if given hints through talk of relative frequencies, this would not prove that they had no problem of base rate neglect.