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The target backsheath field acceleration mechanism is one of the main mechanisms of laser-driven proton acceleration (LDPA) and strongly depends on the comprehensive performance of the ultrashort ultra-intense lasers used as the driving sources. The successful use of the SG-II Peta-watt (SG-II PW) laser facility for LDPA and its applications in radiographic diagnoses have been manifested by the good performance of the SG-II PW facility. Recently, the SG-II PW laser facility has undergone extensive maintenance and a comprehensive technical upgrade in terms of the seed source, laser contrast and terminal focus. LDPA experiments were performed using the maintained SG-II PW laser beam, and the highest cutoff energy of the proton beam was obviously increased. Accordingly, a double-film target structure was used, and the maximum cutoff energy of the proton beam was up to 70 MeV. These results demonstrate that the comprehensive performance of the SG-II PW laser facility was improved significantly.
Dopamine D2 receptors (D2R) are the primary target of antipsychotic drugs and have been shown to regulate Akt/glycogen synthase kinase-3b (GSK-3b) signaling through scaffolding protein b-arrestin 2.
Objective
In the present study, we researched the effects of saikosaponin B1 on the b-arrestin 2-mediated Akt/GSK-3b pathway in human neuroblastoma cell lineSH-SY5Y cells.
Aims
To determine whether saikosaponin B1 affected neuronal morphology in human neuroblastoma cell line SH-SY5Y cells.
Methods
We investigated the effects of saikosaponin B1 on neurite outgrowth using immunostaining. We examined the effects of saikosaponin B1 on Akt and GSK-3b and its well-known downstream regulators, cAMP response element-binding protein (CREB), brain-derived neurotrophic factor (BDNF), and Bcl-2 levels using Western blot analysis.
Results
Saikosaponin B1 was found to enhance neurite outgrowth. Small interfering RNA (siRNA) for b-arrestin 2 knockdown blocked the increase in saikosaponin B1-induced neurite outgrowth. Furthermore, saikosaponin B1 increased the levels of Akt and GSK-3b phosphorylation. The elevation of Akt phosphorylation induced by saikosaponin B1 was reduced by b-arrestin 2 siRNA. Moreover, saikosaponin B1 effectively increased the levels of phospho-CREB, BDNF, and Bcl-2.
Conclusion
Together, these results suggest that regulation of the b-arrestin 2-dependent pathway via blockade of the D2R in SH-SY5Y cells is one mechanism underlying the neuroprotective effect of saikosaponin B1.
Studying the distribution of anatomical abnormalities over the entire cortical surface can help to identify key neural circuits implicated in generating symptoms of neuropsychiatric disorders. There is a significant inconsistency among studies investigating the neuroanatomy of obsessive–compulsive disorder (OCD) because of the confounding influence of co-morbid depression and medication use and the lack of unbiased estimation of whole-brain morphometric changes. It is also unknown whether the distinct surface anatomical properties of thickness, surface area and gyrification, which collectively contribute to grey matter volume (GMV), are independently affected in OCD.
Method
The cortical maps of thickness, gyrification and surface areal change were acquired from 23 unmedicated OCD patients and 20 healthy controls using an unbiased whole-brain surface-based morphometric (SBM) method to detect regional changes in OCD. Subcortical structures were not assessed in this study.
Results
Patients showed a significant increase in the right inferior parietal cortical thickness. Significant increases in gyrification were also noted in the left insula, left middle frontal and left lateral occipital regions extending to the precuneus and right supramarginal gyrus in OCD. Areal contraction/expansion maps revealed no significant regional differences between the patients and controls. In patients, gyrification of the insula significantly predicted the symptom severity measured using Yale–Brown Obsessive–Compulsive Scale (YBOCS).
Conclusions
An alteration in the cortical surface anatomy is an important feature of OCD seen in unmedicated samples that relates to the severity of the illness. The results underscore the presence of a neurodevelopmental aberration underlying the pathophysiology of OCD.
The mechanism of continental growth of the Altaids is currently under debate between models invoking continuous subduction-accretion or punctuated accretion by closure of multiple ocean basins. We use the Yueyashan–Xichangjing ophiolite belt of the Beishan collage (southern Altaids) to constrain the earliest oceanic crust in the southern Palaeo-Asian Ocean. Five lithotectonic units were identified from S to N: the Huaniushan block, a sedimentary passive margin, the structurally incoherent Yueyashan–Xichangjing ophiolite complex, a coherent sedimentary package and the Mazongshan island arc with granitic rocks. We present a structural analysis of the accretionary complex, which is composed of the incoherent ophiolitic melange and coherent sedimentary rocks, to work out the tectonic polarity. A new weighted mean 206Pb–238U age of 533 ± 1.7 Ma from a plagiogranite in the Yueyashan–Xichangjing ophiolite indicates that the ocean floor formed in early Cambrian time. Furthermore, we present new geochemical data to constrain the tectonic setting of the Yueyashan–Xichangjing ophiolite. The Yueyashan–Xichangjing ophiolite was emplaced as a result of northward subduction of an oceanic plate beneath the Mazongshan island arc to the north in late Ordovician to early Silurian time. Together with data from the literature, our work demonstrates that multiple overlapping periods of accretion existed in the Palaeozoic in the northern and southern Altaids. Therefore, a model of multiple accretion by closure of several ocean basins is most viable.
In Western countries, a history of major depression (MD) is associated with reports of received parenting that is low in warmth and caring and high in control and authoritarianism. Does a similar pattern exist in women in China?
Method
Received parenting was assessed by a shortened version of the Parental Bonding Instrument (PBI) in two groups of Han Chinese women: 1970 clinically ascertained cases with recurrent MD and 2597 matched controls. MD was assessed at personal interview.
Results
Factor analysis of the PBI revealed three factors for both mothers and fathers: warmth, protectiveness, and authoritarianism. Lower warmth and protectiveness and higher authoritarianism from both mother and father were significantly associated with risk for recurrent MD. Parental warmth was positively correlated with parental protectiveness and negatively correlated with parental authoritarianism. When examined together, paternal warmth was more strongly associated with lowered risk for MD than maternal warmth. Furthermore, paternal protectiveness was negatively and maternal protectiveness positively associated with risk for MD.
Conclusions
Although the structure of received parenting is very similar in China and Western countries, the association with MD is not. High parental protectiveness is generally pathogenic in Western countries but protective in China, especially when received from the father. Our results suggest that cultural factors impact on patterns of parenting and their association with MD.
To research the protective effect of melatonin against gentamicin ototoxicity.
Methods:
Guinea pigs were randomly divided into four groups. The first group received intramuscular gentamicin (120 mg/kg body weight/day) for 17 days. Over the same time period, a second group simultaneously received intramuscular gentamicin (120 mg/kg body weight/day) plus (on the other side) intramuscular melatonin (0.3 ml kg body weight/day). Two groups of controls were treated for 17 days with either intramuscular melatonin or intramuscular saline. After the 17 days, each animal underwent distortion product otoacoustic emission testing (both ears). The guinea pigs were sacrificed by decapitation just after the final injection. Their cochleae were used to produce a tissue section, surface preparation and scanning electron microscope preparation.
Results:
Distortion product otoacoustic emission testing indicated gentamicin-induced hearing loss at 3, 4, 6 and 8 kHz in gentamicin-treated animals. Animals receiving melatonin co-therapy had significantly attenuated hearing loss and their cochleae showed lower rates of outer hair cell loss (comparing the same cochlear turns), compared with gentamicin-treated animals (p < 0.01).
Conclusion:
These findings confirm the occurrence of outer hair cell loss after gentamicin treatment, and the attenuation of such loss following simultaneous melatonin injection, using the method of morphological evaluation. These results suggest that melatonin protects against gentamicin ototoxicity by interfering with cytotoxic mechanisms.
The structure and magnetic properties of the nanoparticles of immiscible
system Co20Cu80 prepared by means of arc-discharge, have been
studied in detail. The diameters of the particles are about 20 ~ 30 nm
and a core/shell structure forms. The cores are Co-Cu solutions, which show
some small Co precipitates, encapsulated with a shell of cupper oxide or
cobalt oxide as observed by means of high-resolution transmission electron
microscope (HRTEM) and energy dispersive X-ray (EDS). The loop shift in the
hysteresis loop indicates the existence of the exchange bias between
ferromagnetic and antiferromagnetic components at low temperatures. A block
temperature about 180 K has been observed for as-deposited nanoparticles.
For the annealed nanoparticles, the thermal magnetization at low
temperatures is satisfied with Bloch's law.
Simulation and experimental work that compare the performance of straight and tapered monocapillaries when used with laboratory x-ray sources are reported. Detailed simulations for various taper profiles give several important conclusions for optimizing the design of a tapered monocapillary. Several tapered monocapillaries were prepared. With a 16W x-ray source, beam intensities of 4×105 photon/sec/μm2 and 3×105photon/sec/μm2 of Cu Kα x rays were obtained from the tapered monocapillaries for output diameters of 8μm and 3.5μm, respectively. These intensities are 1.4 and 1.5 times that obtained from straight capillaries with the same output beam sizes at the experimental set-up optimized for a straight capillary. In addition to the gain in x-ray flux, the tapered monocapillaries produce output beams with significantly reduced high energy bremsstrahlung radiation and increased flux stability with respect to shifts of the x-ray source spot.
Very recently a new technique named multi-ion-bram reactive cosputtering(MIBRECS) was developed for preparing multi-component metal oxide thin films. Epitaxial or highly oriented (Pb,La) TiO3 thin films sputtered from pure metals of lead, titanium and lathanium were deposited by using this technique. In order to consummate the technique and to study the mechanism of reactive cosputtering, a general model of multi-ion-beam reactive cosputter-ing was proposed for the first time based on the well-known gas kinetics under stable sputtering circumstances, and a computer numerical simulation of the model was carried out with the parameters adopted in our experiments. The relationships among the sputtering ratioes of the targets, and the coverage ratioes of simple substances and oxides of the target metals on substrate surface with the total reactive gas flux and the densities of the sputtering ion beam were obtained respectively, and the hysteresis effect of the characteristic of reactive sputtering and the interactions during multi-ion-beam reactive cosputtering processes were also obtained. The numerical simulation results are at least qualitively in agreement with the experiments.
Epitaxial Si layers have been grown under a variety of growth conditions on CoSi2 (001) by molecular beam epitaxy (MBE). The structural properties of the Si overgrowth were studied by in-situ Reflection High Energy Electron Diffraction (RHEED), as well as ex-situ MeV4He+ ion channeling and High Resolution Transmission Electron Microscopy (HRTEM). Strong influences of the CoSi2 surface reconstruction on the Si overgrowth have been observed. RHEED studies show islanding growth of Si on the CoSi2 (001) (3/√2 × √2)R45 reconstructed surface, but smooth growth of Si on the CoSi2 (001) {√2 × √2)R45 reconstructed surface, under the same growth conditions. The growth of Si on thin layers of CoSi2 (2nm-6nm) with (√2 × √2)R45 reconstructed surface at 460°C results in high crystalline quality for the Si top layer, as indicated by good channeling minimum yield (Xmin < 6%), but cross-sectional TEM shows that the CoSi2 layers are discontinuous. We also report preliminary results on Si grown on a 2 × 2 reconstructed CoSi2 (001) surface.
Here, we report on our efforts to engineer Si substrates for growth of compound semiconductors through the use of suitable epitaxial buffer layers of CaF2, SrF2, and BaF2 using a recently installed dual growth chamber MBE system. We have also developed new graphite-heater K-cells which have demonstrated reliable, high temperature deposition of the fluorides with excellent uniformity across substrates up to 6 inches in diameter. Excellent epitaxial quality (Xmin<5.0%) and smooth surface morphologies have been achieved for epitaxial CaF2 and SrF2 grown directly on Si (111) and BaF2 grown directly on Si (001). The BaF2 is (111) oriented on the Si (001) substrates with one of the {110} planes of the BaF2 aligned with the (110) plane in the Si (001). PbS1−xSex of excellent epitaxial quality has recently been demonstrated on the BaF2 (111)/Si (001) films. Comparable epitaxial quality has been demonstrated for CaF2 grown on Si (001) substrates using a two step growth method. We also report on preliminary results on epitaxial mixed fluoride growth on Si (111) and Si (001) substrates.
A 3-dimensional phase diagram is introduced to describe the dependence of
the RHEED pattern from GaAs(111)B surface on growth conditions. The 2×2,
transitional(1×1), and √19×,√19 surface reconstructions correspond to
different zones in the phase diagram. A equation is given for the planes
that separate these zones, which fit experimental data well. Homoepitaxial
films on GaAs(111)B grown in the 2×2 region generally have bad crystal
quality as determined by the ion channeling, and growth in the √19×√19
region generally yields rough surface morphology. At higher substrate
temperatures (∼ 650 °C), featureless films with minimum ion channeling
yields of less than 4% are achieved.
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