OBJECTIVES/GOALS: Assess molecular and cellular mechanisms of allograft loss in kidney biopsies using digital spatial profiling and clinical outcomes data. METHODS/STUDY POPULATION: Patients with chronic allograft dysfunction (CGD), enrolled in the Deterioration of Kidney Allograft Function (DeKAF) study, with or without eventual allograft loss, were included. CGD was defined as a >25% increase in creatinine over 3 months relative to a baseline. Kidney biopsy tissue was assessed by Nanostring GeoMX digital spatial profiling (DSP) after staining with anti-pan-cytokeratin, anti-CD45, anti-CD68, Syto-13, to identify specific cell populations, and Nanostring’s Whole Transcriptome Atlas (WTA), to quantify the distribution of transcripts across the biopsy. Up to 14 regions of interest (ROIs) were selected, with or without glomerulus. CIBERSORT was used to perform cell deconvolution. Clinical and outcomes data were from the DeKAF study and United States Renal Data System. RESULTS/ANTICIPATED RESULTS: Macrophage (M1) cell population abundance was significantly different in ROIs with glomerulus between graft loss and no graft loss. Principle component analysis of differentially expressed genes resulted in transcriptomes in ROIs that cluster together by clinical outcome of graft loss or no graft loss. There were 203 DEGs in ROIs with glomerulus that were different by graft loss or no graft loss. By pathway analysis, these 203 DEGS were enriched in the T-cell activation, integrin signaling and inflammation pathways. DISCUSSION/SIGNIFICANCE: DSP of kidney allograft biopsies allows for the identification and quantification of specific cell types, such as macrophages and molecular transcripts as potential drug targets. This data can be used to understand mechanisms of kidney allograft loss and may lead to improved immune suppression in kidney transplant recipients.

Past research has shown that many people prefer natural foods and medicines over artificial counterparts. The present study focused on examination of aversive events and hazards. Preferences were compared by having subjects consider pairs of scenarios, one natural and one artificial, matched in negative outcome and severity. Pairings were also rated along several dimensions of risk perception such as dangerousness, scariness, likelihood, and fairness. As hypothesized, natural hazards were consistently preferred to functionally identical artificial ones. Additionally, natural hazards tended to be considered less scary and dangerous, but not necessarily more unfair or unlikely than equivalent artificial counterparts. Results are discussed in terms of risk perception, and how that can lead to people diminishing risks associated with natural hazards.

The role that physical attractiveness and fluctuating asymmetry (FA), a measure of developmental instability, play in self-perception and peer associations were explored in a well-studied cohort of Jamaican children using a novel research paradigm where subjects were already known to each other for extensive periods of time. The results showed that how attractive a child was perceived by others was significantly positively correlated with self-ratings of attractiveness. Contrary to findings from WEIRD (Western, Educated, Industrialized, Rich, Democratic) samples, the study found a reversal in the sex differences in self-perceived attractiveness and self-esteem, where Jamaican females rate themselves more attractive and report higher self-esteem than do males. Attractiveness also predicts overall popularity, as measured by desirability as a friend and the percentage of peers who choose an individual as a friend. Attractive individuals of both sexes were chosen more often as ‘friends’. A significant correlation was also found between an individual’s FA and the average FA of those chosen as friends. However, the effect was primarily due to preferences by males for female friends possessing similar levels of FA, which could be an effective strategy in reducing future mating effort.

Heteroplasmy is the existence of multiple mitochondrial DNA haplotypes within the cell. Although the number of reports of heteroplasmy is increasing for arthropods, the occurrence, number of variants, and origins are not well studied. In this research, the occurrence of heteroplasmy was investigated in Thrips tabaci, a putative species complex whose lineages can be distinguished by their mitochondrial DNA haplotypes. The results from this study showed that heteroplasmy was due to the occurrence of mitochondrial cytochrome oxydase I (mtCOI) haplotypes from two different T. tabaci lineages. An assay using flow cytometry and quantitative real-time PCR was then used to quantify the per cell copy number of the two mtCOI haplotypes present in individuals exhibiting heteroplasmy from nine geographically distant populations in India. All of the T. tabaci individuals in this study were found to exhibit heteroplasmy, and in every individual the per cell copy number of mtCOI from lineage 3 comprised 75–98% of the haplotypes detected and was variable among individuals tested. There was no evidence to suggest that the presense of lineage-specific haplotypes was due to nuclear introgression; however, further studies are needed to investigate nuclear introgression and paternal leakage during rare interbreeding between individuals from lineages 2 and 3.

The landscapes of northern New England and adjacent areas of Canada changed greatly between 14,000 and 9000 yr B.P.: deglaciation occurred, sea levels and shorelines shifted, and a vegetational transition from tundra to closed forest took place. Data from 51 14C-dated sites from a range of elevations were used to map ice and sea positions, physiognomic vegetational zones, and the spread of individual tree taxa in the region. A continuum of tundra-woodland-forest passed northeastward and northward without major hesitation or reversal. An increased rate of progression from 11,000 to 10,000 yr B.P. suggests a more rapid warming than in the prior 2000–3000 yr. Elevational gradients controlled the patterns of deglaciation and vegetational change. The earliest spread of tree taxa was via the lowlands of southern Vermont and New Hampshire, and along a coastal corridor in Maine. Only after 12,000 yr B.P. did the taxa spread northward through the rest of the area. Different tree species entered the southern part of the area at different times and continued their spread at different rates. The approximate order of arrival follows: poplars (13,000–12,000 yr B.P. in the south), spruces, paper birch, and jack pine, followed by balsam fir and larch, and possibly ironwood, ash, and elm, and somewhat later by oak, maple, white pine, and finally hemlock (10,000–9000 yr B.P. in the south).

The judicious selection of sites for paleovegetational and paleoclimatic studies permits paleoecologists to answer specific research questions that go beyond primary descriptions of past vegetation. We present a model that describes the relationship between basin size and pollen source area and predicts the proportions of local, extralocal, and regional pollen sampled by lake basins of different size. The distinctive sampling properties of lakes, peats, and small hollows can be exploited to provide details of pattern in paleovegetation so long as attention is given to the limitations and problems of these types of sites. Combinations of site types in a single study most fully exploit the information contained in sediments.

Fláajökull is a non-surging outlet glacier draining the south-eastern part of the Vatnajökull, southeast Iceland. Fláajökull was stationary or advanced slightly between 1966 and 1995 and formed a prominent end moraine. Glacial retreat since then has revealed a cluster of 15 drumlins. This study focuses on the morphology and sedimentology of the drumlins. They are 100–600 m long, 40–130 m wide, and have cores of glaciofluvial sediment or till. The drumlins are draped by ~1 m thick, massive subglacial traction till. The glacier forefield is characterized by a number of arcuate and saw-tooth, terminal and recessional moraine ridges, overridden moraines with fluted surfaces, and glaciofluvial outwash. Some of the drumlins extend towards the 1995 end moraine but terminate abruptly at the moraine and are not observed in front of it. This suggests that they were formed sub-marginally during the 1966–1995 terminal position. The sedimentary structure of the drumlins is best explained by the sticky spot model. Dating and dendrochronological analyses of birch logs found on the surface of one of the drumlins indicate that the valley was forested about 2100 calendar year BP, after which the glacier started to reform, possibly due to an abrupt change in climate.

Cutworms are excellent insects for investigations on physiology, behaviour, and toxicology in the laboratory. Although they are often plentiful and easily obtained in the field during outbreaks their use as laboratory animals is dependent upon satisfactory methods of rearing. Cutworms vary in life history and feeding habits, and many species require distinctive rearing techniques.