Public attitude surveys provide invaluable insights into societal views on women’s rights, democracy and other critical issues. However, many research studies do not account for biases introduced by the gender of the interviewer, which can distort estimates of public opinion and key relationships among covariates of interest. This article examines gender-of-interviewer effects on public support for women’s rights to work, own and inherit land, as well as support for democracy and feelings of closeness to opposition (versus ruling) parties, using Afrobarometer data from 34 African countries. In line with prevailing conservative social norms in Africa, the analysis reveals significant gender-of-interviewer effects, with respondents reporting more gender-unequal attitudes when interviewed by male interviewers. Additionally, gender-of-interviewer effects appear in responses to questions on support for democracy and feelings of closeness to opposition (versus ruling) parties, with respondents more likely to voice pro-democratic attitudes and close affiliation with opposition parties to male interviewers, regardless of their own gender. These findings highlight the importance of accounting for such biases to ensure the validity of public opinion research and analyses based on these political variables.