The water buffalo farm with intensive breeding techniques promotes the spread of lice (Haematopinus tuberculatus), leading to a reduction in meat and milk productions. Current control measures include the use of chemicals, which in the long-term lead to the development of resistance. Given the lack of alternative solutions, the aim of this study was to evaluate the repellent effect of a low impact by-product, the wood vinegar (WV), against H. tuberculatus using a new in vitro bioassay test. The test consisted of a glass Petri dishes subdivided in 3 separate areas. WV was tested at different concentrations (100%, 75%, 50%, 25%). Each of the 3 replicates was performed with 10 adults, at 27 °C and 75% relative humidity. Observations were made 5, 15, 30, 60, 90, 120 and 180 min after application to evaluate movement, the living/dead ratio and the ability to overcome the obstacle area of the lice, in terms of comparative avoidance (%). Additional in vitro cytotoxicity tests were performed. The test performed showed a repellent effect of 77% for the 100% WV concentration against lice of both sexes 30 min after exposure, similar (P < 0.05) to the repellent effect of deltamethrin (77%). The in vitro bioassay developed during the experiment proved to be reliable and can be used to evaluate the repellent effect of new molecules against lice before testing them in vivo. Furthermore, WV has a potential to be a valid tool for the control of buffalo pediculosis, although in vivo studies have to be undertaken.

Objectives/Goals: Transmission-blocking vaccines hold promise for malaria elimination by reducing community transmission. But a major challenge that limits the development of efficacious vaccines is the vast parasite’s genetic diversity. This work aims to assess the genetic diversity of the Pfs25 vaccine candidate in complex infections across African countries. Methods/Study Population: We employed next-generation amplicon deep sequencing to identify nonsynonymous single nucleotide polymorphisms (SNPs) in 194 Plasmodium falciparum samples from four endemic African countries: Senegal, Tanzania, Ghana, and Burkina Faso. The individuals aged between 1 and 74 years, but most of them ranged from 1 to 19 years, and all presented symptomatic P. falciparum infection. The genome amplicon sequencing was analyzed using Geneious software and P. falciparum 3D7 as a reference. The SPNs were called with a minimum coverage of 500bp, and for this work, we used a very sensitive threshold of 1% variant frequency to determine the frequency of SNPs. The identified SNPs were threaded to the crystal structure of the Pfs25 protein, which allowed us to predict the impact of the novel SNP in the protein or antibody binding. Results/Anticipated Results: We identified 26 SNPs including 24 novel variants, and assessed their population prevalence and variant frequency in complex infections. Notably, five variants were detected in multiple samples (L63V, V143I, S39G, L63P, and E59G), while the remaining 21 were rare variants found in individual samples. Analysis of country-specific prevalence showed varying proportions of mutant alleles, with Ghana exhibiting the highest prevalence (44.6%), followed by Tanzania (12%), Senegal (11.8%), and Burkina Faso (2.7%). Moreover, we categorized SNPs based on their frequency, identifying dominant variants (>25%), and rare variants (Discussion/Significance of Impact: We identified additional SNPs in the Pfs25 gene beyond those previously reported. However, the majority of these newly discovered display low variant frequency and population prevalence. Further research exploring the functional implications of these variations will be important to elucidate their role in malaria transmission.

Anticipating future migration trends is instrumental to the development of effective policies to manage the challenges and opportunities that arise from population movements. However, anticipation is challenging. Migration is a complex system, with multifaceted drivers, such as demographic structure, economic disparities, political instability, and climate change. Measurements encompass inherent uncertainties, and the majority of migration theories are either under-specified or hardly actionable. Moreover, approaches for forecasting generally target specific migration flows, and this poses challenges for generalisation.

In this paper, we present the results of a case study to predict Irregular Border Crossings (IBCs) through the Central Mediterranean Route and Asylum requests in Italy. We applied a set of Machine Learning techniques in combination with a suite of traditional data to forecast migration flows. We then applied an ensemble modelling approach for aggregating the results of the different Machine Learning models to improve the modelling prediction capacity.

Our results show the potential of this modelling architecture in producing forecasts of IBCs and Asylum requests over 6 months. The explained variance of our models through a validation set is as high as 80%. This study offers a robust basis for the construction of timely forecasts. In the discussion, we offer a comment on how this approach could benefit migration management in the European Union at various levels of policy making.

This study critically examines the implications of integrating Indigenous relational worldviews into the water governance framework of the Saskatchewan River Delta. Using a relational theoretical framework and community-based participatory research methodology, both Indigenous community members and non-Indigenous researchers collectively examine the negative impacts of Western water governance policies and practices on the Métis community residing in Cumberland House, located in northeast Saskatchewan, Canada. Through Indigenous traditional water story-sharing methods with Indigenous Elders and Knowledge Keepers, our focus centres on Indigenous interpretations and ways of knowing the Delta. The community highlighted the pervasive influence of power dynamics and political agendas in the governance of the Delta. As such, we emphasise the necessity of challenging settler colonial systems and structures and reinvigorating Indigenous worldviews for water governance. By doing so, we advocate for the advancement of Indigenous sovereignty and self-determination in their relationship with land and water, thereby promoting the meaningful implications of the Truth and Reconciliation Commission Calls to Action.

Cystic echinococcosis (CE), caused by the larval stage of the cestode Echinococcus granulosus, is one of the most widespread zoonoses in Mediterranean countries. Baiting not-owned dogs with praziquantel (PZQ), due to their key role in the maintaining the transmission of CE, currently appears to be the most effective way to limit the transmission of CE, as well as an important aspect to introduce for the control of this parasitic disease. Therefore, this study aims to test 3 types of PZQ-based baits by evaluating different parameters (integrity over time, attractiveness and palatability for dogs, and mechanical resistance after release to different altitudes) and the bait acceptance in field by target animals, i.e. not-owned dogs, by using camera traps. The double PZQ-laced baits (with a double layer of highly palatable chews) showed the greatest resistance in the environment while also preserving the attractiveness and palatability up to 10 days, also withstood heights of 25 m, thus resulting as the most suitable also for drone delivery. The results on the field showed that most of the baits were consumed by not-owned dogs (82.2%), while the remaining were consumed by wild boars (8.9%), foxes (6.7%), badgers (1.1%) and hedgehogs (1.1%), confirming the specific and high attractiveness of the double PZQ-laced baits for the target population and highlights how an anthelmintic baiting programme may be a viable tool for the management of E. granulosus among free-ranging dog populations in endemic rural areas.