In this work, we focus on the period-luminosity relation (PLR) of δ Sct stars, in which mode excitation and selection mechanisms are still poorly constrained, and whose structure and oscillations are affected by rotation. We review the PLRs in the recent literature, and add a new inference from a large sample of δ Sct. We highlight the difficulty in identifying the fundamental mode and show that rotation-induced surface effects can impact the measured luminosities, explaining the broadening of the PLR. We derive a tight relation between the low-order large separation and the fundamental radial mode frequency (F0) that holds for rotating stars, thus paving the way towards mode identification. We show that the PLRs we obtain for different samples are compatible with each other and with the recent literature, and with most observed δ Sct stars when taking rotation effects into account. We also find that the highest-amplitude peak in the frequency spectrum corresponds to the fundamental modein most δ Sct, thus shedding some light on their elusive mode selection mechanism.

By assuming that the Kirchhoff term has $K$ degeneracy points and other appropriated conditions, we have proved the existence of at least $K$ positive solutions other than those obtained in Santos Júnior and Siciliano [Positive solutions for a Kirchhoff problem with vanishing nonlocal term, J. Differ. Equ. 265 (2018), 2034–2043], which also have ordered $H_{0}^{1}(\Omega )$-norms. A concentration phenomena of these solutions is verified as a parameter related to the area of a region under the graph of the reaction term goes to zero.

We study Lotka–Volterra models with fractional Laplacian. To do this we study in detail the logistic problem and show that the sub–supersolution method works for both the scalar problem and for systems. We apply this method to show the existence and non-existence of positive solutions in terms of the system parameters.

The Pliocene and Pleistocene continental sedimentary records of the western sector of the Granada Basin, southern Spain, consist of alternating fluvial and lacustrine/palustrine sediments. Two Quaternary sections from this sector have been sampled: Huétor Tájar and Tojaire. They have yielded remains of rodents, insectivores and lagomorphs. The presence in the Huétor Tájar and Tojaire sections of Mimomys, Apodemus atavus, Castillomys rivas and two different species of Allophaiomys, indicates an Early Pleistocene age. These deposits, which are related to a fluvio-lacustrine system, can be differentiated from an older (Pliocene) braided fluvial system. Their dating has important repercussions on the paleogeographic reconstruction of the basin. The conditions inferred from the ecological preferences of the small mammal associations are wet and cold. These associations suggest a predominance of open herbaceous habitats, followed by forested habitats; semiaquatic habitats are the least represented.

The Genus Micromys includes a single extant species, Micromys minutus (Pallas, 1771), which lives in Europe and North Asia. This genus is known in the fossil record since the late Miocene; eight fossil species have been described in Europe and Asia, most of them of late Miocene and early Pliocene age. The evolution of this genus during the late Pliocene is barely known. Although it is present in numerous localities of this age, remains of Micromys are usually scarce and generally assigned to the species M. minutus or M. praeminutus Kretzoi, 1959.

Very few large studies in Latin America have evaluated the association between waist:height ratio (W-HtR) and cardiometabolic risk in children and adolescents. Further, multivariable analyses verifying the independence of located subcutaneous fat have not been conducted so far. The aim of this study was to evaluate the associations of W-HtR and waist circumference (WC) with metabolic syndrome abnormalities and high LDL-cholesterol levels in schooled adolescents before and after adjusting for trunk skinfolds and BMI. The sample consisted of 831 boys and 841 girls aged 10–17 years. Biochemical, blood pressure and anthropometrical variables were measured. Age- and sex-specific quartiles of W-HtR and WC were used in Poisson regression models to evaluate the associations. High WC values (highest quartile v. quartiles 1–3) were associated with high TAG levels in both sexes (prevalence ratio, boys: 2·57 (95 % CI 1·91, 3·44); girls: 1·92 (95 % CI 1·49, 2·47); P<0·05), and with high blood pressure specifically in female adolescents (3·07 (95 % CI 1·58, 5·98); P<0·05), independently of trunk skinfolds or BMI (P<0·05). Associations of high WC with high fasting glucose (boys), low HDL-cholesterol and having at least two abnormalities did not remain significant in most of the adjustments for trunk skinfolds or BMI (P>0·05). High W-HtR (highest quartile v. quartiles 1–3) was only independently associated with high TAG in female adolescents (1·99 (95 % CI 1·55, 2·56); P<0·05). In conclusion, WC showed better association with cardiometabolic risk than W-HtR in the children of this study. This observation does not support W-HtR as a relevant adiposity marker for cardiovascular and metabolic risk in adolescence.

The genus Stephanomys (Muridae, Rodentia) is one of the most common elements in the late Miocene to Early Pleistocene mammal faunas from the Ibero-Occitan region. Its geographic distribution is limited to this area with only two mentions in the late Miocene of Italy (de Giuli, 1989) and Algeria (Coiffait et al., 1985). The genus has been subject of numerous studies, some of them suggesting different interpretations on the phylogenetic relationships between the various described species (Gmelig-Meyling and Michaux, 1973; Cordy, 1978; Adrover, 1986; Bachelet and Castillo-Ruiz, 1990; Aguilar et al., 1993). The most extensive and significant study of the genus is the Ph.D. dissertation of Cordy (1976), who studied in detail several samples of Stephanomys, analyzed the changes observed in successive populations and defined four species (S. medius, S. michauxi, S. thaleri and S. progressus), which are considered as nomina nuda because this work was never published. Only one of these species, S. thaleri from the French locality of Seynes, was validated later by López-Martinez et al. (1998).

A rich and diverse micromammal fauna from the late Turolian (MN13) locality of Negratín-1 (Guadix Basin, southern Spain) is described. The faunal list of this site includes Apodemus gudrunae, Occitanomys alcalai, Stephanomys dubari, Paraethomys meini, Myocricetodon jaegeri, Debruijnimys almenarensis, Apocricetus alberti, Ruscinomys sp., Eliomys sp., Atlantoxerus sp., Parasorex ibericus, and Soricidae indet. This is the most nearly complete mammal fauna from the Miocene of the Guadix Basin and allows precise correlations with localities from other Iberian areas. In addition, some of the taxa identified in Negratín-1 are useful as palaeoecological indicators (Myocricetodon, Debruijnimys, Atlantoxerus), evidencing warm and dry climatic conditions. But the principal interest of the fauna from Negratín-1 is the presence of several species of African origin, the gerbillids Debruijnimys almenarensis and Myocricetodon jaegeri, which are recognized for the first time in Europe. We also ascribe to M. jaegeri the population from the upper Turolian karst infilling of Almenara-M. This finding constitutes new evidence for faunal exchanges between North Africa and the Iberian Peninsula during the Messinian Salinity Crisis.

Flavonoids are polyphenolic compounds that are widespread in nature, and consumed as part of the human diet in significant amounts. The aim of the present study was to test the intestinal anti-inflammatory activity of apigenin K, a soluble form of apigenin, in two models of rat colitis, namely the trinitrobenzenesulfonic acid (TNBS) model and the dextran sulphate sodium (DSS) model. Apigenin K (1, 3 and 10 mg/kg; by the oral route; n 4–6 per group) was administered as a pre-treatment to rats with TNBS and DSS colitis, and colonic status was checked by macroscopic and biochemical examination. Apigenin K pre-treatment resulted in the amelioration of morphological signs and biochemical markers in the TNBS model. The results demonstrated a reduction in the inflamed area, as well as lower values of score and colonic weight:length ratio compared with the TNBS group. Myeloperoxidase (MPO) activity was reduced by 30 % (P< 0·05). Moreover, apigenin K pre-treatment ameliorated morphological signs and biochemical markers in the DSS model. Thus, macroscopic damage was significantly reduced and the colonic weight:length ratio was lowered by approximately 10 %, while colonic MPO and alkaline phosphatase activities were decreased by 35 and 21 %, respectively (P< 0·05). Apigenin K pre-treatment also tended to normalise the expression of a number of colonic inflammatory markers (e.g. TNF-α, transforming growth factor-β, IL-6, intercellular adhesion molecule 1 or chemokine (C–C motif) ligand 2). In conclusion, apigenin K is found to have anti-inflammatory effects in two preclinical models of inflammatory bowel disease.

Twelve accelerator mass spectrometry (AMS) radiocarbon dates from the shell-matrix site of Canímar Abajo (Matanzas, Cuba) are reported. Eleven were obtained directly from human bone collagen in burials and one was obtained from charcoal recovered from a burial context. The site stratigraphy presents two episodes of burial activity separated by a shell midden layer. The AMS dates fall into two compact clusters that correlate remarkably well with the stratigraphy. The older burial dates to between 1380–800 cal BC (2σ) and the younger one to between cal AD 360–950 (2σ). The AMS dates are compared to eight conventional 14C dates previously obtained on shell and charcoal. One of the conventional dates on charcoal (5480–5380 cal BC; 2σ) has been reported as the oldest 14C date in the Caribbean region; its context and reliability are clarified. The suite of AMS dates provides one of the most reliable chronometric dating of a cultural context during this timeframe in Cuba. The correlation of 14C and stratigraphy establishes a solid chronology for investigating the important economic and ritual features of Canímar Abajo.

Purpose: To explore the use of three-dimensional patient-specific cardiovascular models using rapid prototyping techniques (fused deposition modelling) to improve surgical planning in patients with complex congenital heart disease. Description: Rapid prototyping techniques are used to print accurate three-dimensional replicas of patients' cardiovascular anatomy based on magnetic resonance images using computer-aided design systems. Models are printed using a translucent polylactic acid polymer. Evaluation: As a proof of concept, a model of the heart of a 1.5-year-old boy with transposition of the great arteries, ventricular septal defect and pulmonary stenosis was constructed to help planning the surgical correction. The cardiac model allowed the surgeon to evaluate the location and dimensions of the ventricular septal defect as well as its relationship with the aorta and pulmonary artery. Conclusions: Cardiovascular models constructed by rapid prototyping techniques are extremely helpful for planning corrective surgery in patients with complex congenital malformations. Therefore they may potentially reduce operative time and morbi-mortality.

Milk κ-casein-derived bovine glycomacropeptide (GMP) exerts immunomodulatory effects. It exhibits intestinal anti-inflammatory activity in chemically induced models of colitis. However, to validate its clinical usefulness as a nutraceutical, it is important to assess its effects in a model with a closer pathophysiological connection with human inflammatory bowel disease. Therefore, in the present study, we used the lymphocyte-transfer model of colitis in mice and compared the effects of GMP in this model with those obtained in the dextran sulphate sodium (DSS) model. GMP (15 mg/d) resulted in higher body-weight gain and a reduction of the colonic damage score and myeloperoxidase (MPO) activity in Rag1 − / − mice with colitis induced by the transfer of naïve T cells. The colonic and ileal weight:length ratio was decreased by approximately 25 %, albeit non-significantly. GMP treatment reduced the percentage of CD4+ interferon (IFN)-γ+ cells in mesenteric lymph nodes (MLN). The basal production of IL-6 by MLN obtained from the GMP-treated mice ex vivo was augmented. However, concanavalin A-evoked production was similar. The colonic expression of regenerating islet-derived protein 3γ, S100A8, chemokine (C-X-C motif) ligand 1 and IL-1β was unaffected by GMP, while that of TNF-α and especially IFN-γ was paradoxically increased. In the DSS model, GMP also reduced the activity of colonic MPO, but it failed to alter weight gain or intestinal weight:length ratio. GMP augmented the production of IL-10 by MLN cells and was neutral towards other cytokines, except exhibiting a trend towards increasing the production of IL-6. The lower effect was attributed to the lack of the effect of GMP on epithelial cells. In conclusion, GMP exerts intestinal anti-inflammatory effects in lymphocyte-driven colitis.

We study the theoretical properties of the regular spacings found in the oscillation spectra of δ Scuti stars. A linear relation between the large separation and the mean density is predicted to be found in the low-frequency domain (i.e. radial orders spanning from 1 to 8, approximately) of the main-sequence δ Scuti stars' oscillation spectrum. This implies an independent direct measure of the average density of δ Scuti stars, analogous to that of the Sun, and places tight constraints on the mode identification and hence on the stellar internal structure and dynamics, and allows a determination the radii of planets orbiting around δ Scuti stars with unprecedented precision. This opens the way for studying the evolution of regular patterns in pulsating stars, and its relation to stellar structure and evolution.

The aim of the present study was to isolate, identify and characterise novel strains of lactic acid bacteria and bifidobacteria with probiotic properties from the faeces of exclusively breast-fed infants. Of the 4680 isolated colonies, 758 exhibited resistance to low pH and tolerance to high concentrations of bile salts; of these, only forty-two exhibited a strong ability to adhere to enterocytes in vitro. The identities of the isolates were confirmed by 16S ribosomal RNA (rRNA) sequencing, which permitted the grouping of the forty-two bacteria into three different strains that showed more than 99 % sequence identity with Lactobacillus paracasei, Lactobacillus rhamnosus and Bifidobacterium breve, respectively. The strain identification was confirmed by sequencing the 16S–23S rRNA intergenic spacer regions. Strains were assayed for enzymatic activity and carbohydrate utilisation, and they were deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) of the Institute Pasteur and named L. paracasei CNCM I-4034, B. breve CNCM I-4035 and L. rhamnosus CNCM I-4036. The strains were susceptible to antibiotics and did not produce undesirable metabolites, and their safety was assessed by acute ingestion in immunocompetent and immunosuppressed BALB/c mouse models. The three novel strains inhibited in vitro the meningitis aetiological agent Listeria monocytogenes and human rotavirus infections. B. breve CNCM I-4035 led to a higher IgA concentration in faeces and plasma of mice. Overall, these results suggest that L. paracasei CNCM I-4034, B. breve CNCM I-4035 and L. rhamnosus CNCM I-4036 should be considered as probiotic strains, and their human health benefits should be further evaluated.

A hybridization of a high order WENO-Z finite difference scheme and a high order central finite difference method for computation of the two-dimensional Euler equations first presented in [B. Costa and W. S. Don, J. Comput. Appl. Math., 204(2) (2007)] is extended to three-dimensions and for parallel computation. The Hybrid scheme switches dynamically from a WENO-Z scheme to a central scheme at any grid location and time instance if the flow is sufficiently smooth and vice versa if the flow is exhibiting sharp shock-type phenomena. The smoothness of the flow is determined by a high order multi-resolution analysis. The method is tested on a benchmark sonic flow injection in supersonic cross flow. Increase of the order of the method reduces the numerical dissipation of the underlying schemes, which is shown to improve the resolution of small dynamic vortical scales. Shocks are captured sharply in an essentially non-oscillatory manner via the high order shock-capturing WENO-Z scheme. Computations of the injector flow with a WENO-Z scheme only and with the Hybrid scheme are in very close agreement. Thirty percent of grid points require a computationally expensive WENO-Z scheme for high-resolution capturing of shocks, whereas the remainder of grid points may be solved with the computationally more affordable central scheme. The computational cost of the Hybrid scheme can be up to a factor of one and a half lower as compared to computations with a WENO-Z scheme only for the sonic injector benchmark.