Maternal ability (milk and environment) in beef cattle is one of the most important traits influencing weaning weight of the calf, but amount and composition of milk produced by the dam is difficult to measure. The assessment of polymorphisms of candidate genes related to milk composition could indirectly enlighten this perspective. In the present study, the frequency of αs1-casein 1175AG and g26181A>G; κ-casein 13068 (CSN3 AB1) and 13104 (CSN3 AB2) loci, β-lactoglobulin variants 3984GA and 5263CT, Diacyl glycerol transferase K232A, and Stearoyl CoA desaturase A702G and A762G polymorphisms were estimated in Gray and Red Brahman, Nellore, Guzerat, Gir, Indubrazil, and Sardo Negro registered sires from Mexico. Most of the documented favourable alleles were found in low frequency in most of the evaluated breeds, except for β-lactoglobulin, in which the presence of favourable alleles might represent an opportunity for marker-assisted introgression. The relevance of the findings for each variation and implications from the outcomes are discussed.

Climbing aroids, despite their abundance in tropical forests, remain underexplored. This study is focused on species richness, abundance, density, and distribution patterns of climbing aroid community in a lowland rainforest in Los Tuxtlas, Veracruz, Mexico. Over two years, two censuses were conducted across 14 plots, recording 12 aroid species from five genera and their potential hosts. Ontogenetic classes were defined and validated, showing a positive correlation between total plant length/apex height and ontogenetic stage, indicating distinct growth phases. Host size (DBH) was significant predictor of the establishment probability across ontogenetic classes. Vertical distribution varied significantly among species, ranging from Philodendron hederaceum (7 m) to Anthurium flexile (0.88 m), with some species predominantly distributed on specific host sizes. Tree falls impacted specific species. Although limitations, including a short study period, restrict broader generalizations, this research establishes a foundational understanding of climbing aroid ecology and underscores the need for standardized methods and long-term monitoring to elucidate their population dynamics and ecological strategies.

We introduce a versatile high-repetition-rate solid tape target system suitable for relativistic laser-plasma driven secondary sources. We demonstrate the operation and stability monitoring based on a petawatt laser focused at 1 Hz. Experiments were carried out at the VEGA-3 laser system of the Centro de Láseres Pulsados facility where results for different tape materials and thicknesses are presented. Experimental proton spectra were recorded by a Thomson parabola spectrometer and a time-of-flight detector. In addition, non-invasive detectors, such as a target charging monitor and ionization chamber detectors, were tested as metrology for the stability of the source. Degradation of the proton signal at high-repetition-rate operation was observed and it was solved by online optimization of the relative focus position of the target and laser beam parameters. We report the use of the tape target for bursts of 1000 shots at 1 Hz with mean cut-off energies of about 10 MeV in optimized interaction conditions.

Citizen science is becoming very useful in surveying and monitoring biodiversity. Within the European Union LIFE medCLIFFS project, a network of volunteers has been established for the detection and long-term monitoring of invasive plant species that threaten the endemic flora of Mediterranean cliffs in northeastern Spain. Through iNaturalist, volunteers record various data along a series of 1-km transects. Based on the ca. 700 observations collected by volunteers in 2023 (the first year of the project), a simple and visually attractive methodology for assessing the recorded populations has been developed. This method classifies populations into one of three population dynamics categories: (1) propagative behavior (i.e., populations with seedlings or young plants but lacking senescent or dead individuals); (2) senescent behavior (i.e., showing senescent/dead plants but lacking seedlings/juveniles); and (3) a mixed behavior (i.e., containing both). This methodology, whose outputs are easily interpretable as heat maps, allows the collection of large datasets on invasive plants by citizen scientists, with two main purposes: (1) knowing which species are most concerning based on simple, straightforward observations of their population dynamics; and (2) identifying which regions of the study area are more problematic and where management efforts should therefore be directed.

This manuscript showcases the latest advancements in deepImageJ, a pivotal Fiji/ImageJ plugin for bioimage analysis in life sciences. The plugin, known for its user-friendly interface, facilitates the application of diverse pre-trained convolutional neural networks to custom data. The manuscript demonstrates several deepImageJ capabilities, particularly in deploying complex pipelines, three-dimensional (3D) image analysis, and processing large images. A key development is the integration of the Java Deep Learning Library, expanding deepImageJ’s compatibility with various deep learning (DL) frameworks, including TensorFlow, PyTorch, and ONNX. This allows for running multiple engines within a single Fiji/ImageJ instance, streamlining complex bioimage analysis workflows. The manuscript details three case studies to demonstrate these capabilities. The first case study explores integrated image-to-image translation followed by nuclei segmentation. The second case study focuses on 3D nuclei segmentation. The third case study showcases large image volume segmentation and compatibility with the BioImage Model Zoo. These use cases underscore deepImageJ’s versatility and power to make advanced DLmore accessible and efficient for bioimage analysis. The new developments within deepImageJ seek to provide a more flexible and enriched user-friendly framework to enable next-generation image processing in life science.

The aim of this study was to compare the levels of reactive oxygen species (ROS) in Bos taurus and Bos indicus in vitro embryos cryopreserved using either slow freezing or vitrification. Embryos were divided into four groups based on subspecies and freezing method: Bos indicus slow freezing (BiSF; n = 8), Bos indicus vitrification (BiVT; n = 10), Bos taurus slow freezing (BtSF; n = 9), and Bos taurus vitrification (BtVT; n = 6). After thawing, the embryos were incubated with CellRox Green and images were obtained using a confocal microscope. The fluorescence intensity of each cell was measured and expressed as arbitrary units of fluorescence (auf) and compared using a multiple regression and unpaired t-test with α = 0.05. Results showed that subspecies and the freezing method significantly affected auf (P < 0.001; R2 = 0.1213). Bos indicus embryos had higher auf than Bos taurus embryos, whether frozen by slow freezing (67.05 ± 23.18 vs 51.30 ± 16.84, P < 0.001) or vitrification (64.44 ± 23.32 vs 47.86 ± 17.53, P < 0.001). Slow freezing induced higher auf than vitrification in both Bos taurus (51.30 ± 16.84 vs 47.86 ± 17.53, P < 0.001) and Bos indicus (67.05 ± 23.18 vs 64.44 ± 23.32, P < 0.014). In conclusion, Bos taurus embryos had lower ROS levels when frozen using vitrification, while Bos indicus embryos had consistent ROS patterns regardless of the freezing method. However, Bos indicus embryos frozen by slow freezing tended to have a higher number of cells with elevated ROS levels.

Few studies have examined the psychometric properties of the Connor-Davidson Resilience Scale (CD-RISC) in a large adolescent community sample, finding a significant disparity. This study explores the psychometric properties of the CD-RISC among Spanish adolescents by means of exploratory factor analysis (EFA), Rasch analysis, and measurement invariance (MI) across sex, as well as internal consistency and criterion validity. The sample was comprised of 463 adolescents (231 girls), aged 12 to 18 years, who completed the CD-RISC and other measures on emotional status and quality of life. The EFA suggested that the CD-RISC structure presented a unidimensional model. Consequently, shorter unidimensional CD-RISC models observed in the literature were explored. Thus, the Campbell-Sills and Stein CD–RISC–10 showed the soundest psychometric properties, providing an adequate item fit and supporting MI and non-differential item functioning across sex. Item difficulty levels were biased toward low levels of resilience. Some items showed malfunctioning in lower response categories. With regard to reliability, categorical omega was. 82. Strong associations with health-related quality of life, major depressive disorder symptoms, and emotional symptoms were observed. A weak association was found between resilience and the male sex. Campbell-Sills and Stein’s CD–RISC–10 model emerges as the best to assess resilience among Spanish adolescents, as already reported in adults. Thus, independently of the developmental stage, the core of resilience may reside in the aspects of hardiness and persistence.

There is limited information on the antibody responses against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in subjects from developing countries with populations having a high incidence of co-morbidities. Here, we analysed the immunogenicity of homologous schemes using the ChAdOx1-S, Sputnik V, or BNT162b2 vaccines and the effect of a booster dose with ChAdOx1-S in middle-aged adults who were seropositive or seronegative to the SARS-CoV-2 spike protein before vaccination. The study was conducted post-vaccination with a follow-up of 4 months for antibody titre using enzyme-linked immunosorbent assay (ELISA) and pseudovirus (PV) neutralization assays (PNAs). All three vaccines elicited a superior IgG anti-receptor-binding domain (RBD) and neutralization response against the Alpha and Delta variants when administered to individuals with a previous infection by SARS-CoV-2. The booster dose spiked the neutralization activity among individuals with and without a prior SARS-CoV-2 infection. The ChAdOx1-S vaccine induced weaker antibody responses in infection-naive subjects. A follow-up of 4 months post-vaccination showed a drop in antibody titre, with about 20% of the infection-naive and 100% of SARS-CoV-2 pre-exposed participants with detectable neutralization capacity against Alpha pseudovirus (Alpha-PV) and Delta PV (Delta-PV). Our observations support the use of different vaccines in a country with high seroprevalence at the vaccination time.

Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are widely recognised as two prevalent sexually transmitted infections that can have detrimental effects on women’s reproductive health. Previous research has concentrated on studying high-risk populations, resulting in limited epidemiological data regarding the general population. Therefore, the objective of this study was to estimate the prevalence of CT and NG among women attending public primary health care in Tlaxcala, Mexico. The study sample included 2,396 women already participating in the cervical cancer screening programme, from July to November 2014. After obtaining informed consent, the CT and NG tests were conducted on cervical samples, using a nucleic acid amplification test. We estimate the prevalence with 95% confidence intervals (CIs). Women who tested positive were promptly notified and provided with appropriate treatment. In our study population, CT and NG prevalences were 3.2 (95% CI: 2.6–4.0) and 0.01 (95% CI: 0.01–0.03), respectively. CT prevalence was higher in younger women (age < 40), although the results indicate a low prevalence; due to the potentially significant impact of CT and NG on women’s health, we require adequate surveillance, and guaranteeing rapid referral to the correct treatment is a priority for the control of these diseases.

Few published studies exist documenting banana pepper tolerance to clomazone. Therefore, field trials were conducted in 2022 at two Indiana locations [Meigs Horticulture Research Farm and the Pinney Purdue Agricultural Center (PPAC)] to evaluate crop safety in plasticulture-grown banana pepper. The experimental design was a split-plot in which the main plot factor was the clomazone rate (0, 840, and 1,680 g ai ha–1) and the subplot factor was cultivar (‘Pageant’ and ‘Sweet Sunset’). Clomazone was applied over the top of black polyethylene mulch-covered raised beds and their respective bare-ground row middles 1 d prior to transplanting 12 pepper plants per subplot. Data collected included crop injury on a scale from 0% (no injury) to 100% (crop death) at 2, 4, and 6 wk after treatment (WAT), and plant stand. Two harvests were performed in which mature fruits were counted and weighed. Injury presented as interveinal bleaching only at PPAC 2 and 4 WAT. At this location 1,680 g ha–1 clomazone resulted in greater injury to ‘Sweet Sunset’ at 2 and 4 WAT (53% and 15%, respectively) than to ‘Pageant’ (19% and 3%, respectively); however, plant stand and yield were not affected by either clomazone rate. These results suggest that the clomazone rate range currently used for bell pepper (280 to 1,120 g ai ha–1) can be applied prior to transplanting plasticulture-grown banana pepper with minimal crop injury and without reducing yield.

Current live-cell imaging techniques make possible the observation of live events and the acquisition of large datasets to characterize the different parameters of the visualized events. They provide new insights into the dynamics of biological processes with unprecedented spatial and temporal resolutions. Here we describe the implementation and application of a new tool called TrackAnalyzer, accessible from Fiji and ImageJ. Our tool allows running semi-automated single-particle tracking (SPT) and subsequent motion classification, as well as quantitative analysis of diffusion and intensity for selected tracks relying on the graphical user interface (GUI) for large sets of temporal images (X–Y–T or X–Y–C–T dimensions). TrackAnalyzer also allows 3D visualization of the results as overlays of either spots, cells or end-tracks over time, along with corresponding feature extraction and further classification according to user criteria. Our analysis workflow automates the following steps: (1) spot or cell detection and filtering, (2) construction of tracks, (3) track classification and analysis (diffusion and chemotaxis), and (4) detailed analysis and visualization of all the outputs along the pipeline. All these analyses are automated and can be run in batch mode for a set of similar acquisitions.

Ultrafast laser pulses provide unique tools to manipulate magnetization dynamics at femtosecond timescales, where the interaction of the electric field usually dominates over the magnetic field. Recent proposals using structured laser beams have demonstrated the possibility to produce regions where intense oscillating magnetic fields are isolated from the electric field. In these conditions, we show that technologically feasible tesla-scale circularly polarized high-frequency magnetic fields induce purely precessional nonlinear magnetization dynamics. This fundamental result not only opens an avenue in the study of laser-induced ultrafast magnetization dynamics, but also sustains technological implications as a route to promote all-optical non-thermal magnetization dynamics both at shorter timescales – towards the sub-femtosecond regime – and at THz frequencies.