The Lower Jurassic (Toarcian) Posidonienschiefer Formation of southwestern Germany is a classic konservat lagerstätte, yielding some of the world’s best-preserved fossils of marine vertebrates, including ichthyosaurs, thalattosuchian crocodylomorphs, plesiosaurs and fishes. Despite numerous studies concentrating on the taphonomy of ichthyosaurs in this formation, less taphonomic work has focussed on the thalattosuchians of the assemblage. Multiple thalattosuchian species displaying a wide range of body sizes have been recovered. We investigated indicators for seafloor arrival position in thirteen Macrospondylus bollensis and one Platysuchus multiscrobiculatus specimens representing various body sizes using three-dimensional (3D) photogrammetric models. False-colour depth maps were used to interpret the relative topography (depth level) of bone penetration into the sediment and were aligned on the XY plane, making them parallel to the stratigraphic plane. Our results show both headfirst and non-headfirst seafloor arrivals in observed specimens, with headfirst seafloor arrivals exhibiting deeply buried skulls, displacement of select cervical vertebrae and/or characteristic fractures in the cranium and mandible. We (1) interpret seafloor landing types in teleosauroids; (2) recognize and list specific characteristics that are consistently attributed to either a headfirst or non-headfirst seafloor arrival; (3) discuss possible factors that may have contributed to these features, such as body shape and size, substrate and velocity; and (4) provide a new definition for headfirst seafloor arrival that can be readily attributed to other marine vertebrates from various formations. Lastly, our results show that observers must carefully consider how historical specimens might have been prepared, as this may influence taphonomic interpretations.

Foliar-applied postemergence applications of glufosinate are often applied to glufosinate-resistant crops to provide nonselective weed control without significant crop injury. Rainfall, air temperature, solar radiation, and relative humidity near the time of application have been reported to affect glufosinate efficacy. However, previous research may have not captured the full range of weather variability to which glufosinate may be exposed before or following application. Additionally, climate models suggest more extreme weather will become the norm, further expanding the weather range to which glufosinate can be exposed. The objective of this research was to quantify the probability of successful weed control (efficacy ≥85%) with glufosinate applied to some key weed species across a broad range of weather conditions. A database of >10,000 North American herbicide evaluation trials was used in this study. The database was filtered to include treatments with a single postemergence application of glufosinate applied to waterhemp [Amaranthus tuberculatus (Moq.) Sauer], morningglory species (Ipomoea spp.), and/or giant foxtail (Setaria faberi Herrm.) <15 cm in height. These species were chosen because they are well represented in the database and listed as common and troublesome weed species in both corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] (Van Wychen 2020, 2022). Individual random forest models were created. Low rainfall (≤20 mm) over the 5 d before glufosinate application was detrimental to the probability of successful control of A. tuberculatus and S. faberi. Lower relative humidity (≤70%) and solar radiation (≤23 MJ m−1 d−1) on the day of application reduced the probability of successful weed control in most cases. Additionally, the probability of successful control decreased for all species when average air temperature over the first 5 d after application was ≤25 C. As climate continues to change and become more variable, the risk of unacceptable control of several common species with glufosinate is likely to increase.

Foliar-applied postemergence herbicides are a critical component of corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] weed management programs in North America. Rainfall and air temperature around the time of application may affect the efficacy of herbicides applied postemergence in corn or soybean production fields. However, previous research utilized a limited number of site-years and may not capture the range of rainfall and air temperatures that these herbicides are exposed to throughout North America. The objective of this research was to model the probability of achieving successful weed control (≥85%) with commonly applied postemergence herbicides across a broad range of environments. A large database of more than 10,000 individual herbicide evaluation field trials conducted throughout North America was used in this study. The database was filtered to include only trials with a single postemergence application of fomesafen, glyphosate, mesotrione, or fomesafen + glyphosate. Waterhemp [Amaranthus tuberculatus (Moq.) Sauer], morningglory species (Ipomoea spp.), and giant foxtail (Setaria faberi Herrm.) were the weeds of focus. Separate random forest models were created for each weed species by herbicide combination. The probability of successful weed control deteriorated when the average air temperature within the first 10 d after application was <19 or >25 C for most of the herbicide by weed species models. Additionally, drier conditions before postemergence herbicide application reduced the probability of successful control for several of the herbicide by weed species models. As air temperatures increase and rainfall becomes more variable, weed control with many of the commonly used postemergence herbicides is likely to become less reliable.

Beta-lactam therapeutic drug monitoring has been growing in prevalence in the acute care hospital setting. Expansion of its use to outpatient parenteral antimicrobial therapy requires careful consideration of potential logistical and therapeutic barriers.

Design representations play a crucial role in facilitating communication between individuals in design. Sketches and physical prototypes are frequently used to communicate design concepts in early-stage design. However, we lack an understanding of the communicative benefits each representation provides and how these benefits relate to the effort and resources required to create each representation. A mixed-methods study was conducted with 44 participants to identify whether sketches and physical prototypes led to different levels of cognitive load perceived by a communicator and listener and the characteristics that shape their cognitive load during communication. Results showed that listeners perceived higher levels of mental and physical demands when understanding ideas as low-fidelity physical prototypes, as compared to sketches. No significant differences were found in the cognitive load levels of communicators between the two conditions. Qualitative analyses of post-task semi-structured interviews identified five themes relating to verbal explanations and visual representations that shape designers’ cognitive load when understanding and communicating ideas through design representations. Results indicate that designers should be aware of the specific objectives they seek to accomplish when selecting the design representation used to communicate. This work contributes to the knowledge base needed for designers to use design representations more effectively as tools for communication.

In 2019, the prime ministers of the United Kingdom and Australia both declared their intent to ban indirect, or secondary, boycotts. In the United Kingdom, the ban was directed against public bodies engaging in the “boycott, divest, and sanction” (BDS) campaign against Israel. In Australia, the proposed ban was directed against environmental action groups. Research on market-based activism to date has focused primarily on conceptualizing the use of the market by nonstate actors to achieve social change, with less attention paid to the role of the state in these dynamics. State efforts to curtail social movements’ repertoires of contention require careful scrutiny to understand the state's role in legitimizing or delegitimizing political activism and to reveal the complex power dynamics between corporations, social movements, and the state. This article analyzes two key instances of the state declaring an intent to prevent activists from protesting through the market. By investigating how indirect boycotts were problematized by state actors, we aim to reveal the rationale behind the state's intervention in marketplace politics. Our findings indicate that opposition to the political cause behind the boycott, rather than a problematization of the strategy itself, drives state intervention.

While a significant amount of research has documented the importance of design artefacts in design communication, relatively little work has investigated the effect of design artefact quality on the development of a shared understanding between designers. In the current work we focus specifically on sketch quality and the effect of sketch quality on the shared understanding of design dyads. A controlled study with 22 design dyads (44 designers) was conducted to understand the relationship between sketch quality and shared understanding. Results suggest that design artefact quality, measured by sketch understandability, does not predict the shared understanding of a design concept. Our findings hold implications for the fundamental ways in which we evaluate sketch quality and the importance of artefact fidelity for communicative acts.

Prior work has demonstrated that gender identity affects team psychological safety, which is critical to the development of a shared understanding of the task. Further, we know that a shared understanding can increase team cohesion and team performance. Little work has investigated how gender differences affect communicative acts within the context of design, and more specifically how gender differences may affect the development of a shared understanding of the design concept between designers. As a first step towards filling this gap, the current work presents findings from a controlled study conducted at The Pennsylvania State University with 22 design dyads (44 designers). The findings from this study indicate that gender identity within design dyads does not affect participants’ shared understanding of a design concept.

OBJECTIVES/GOALS: Glioblastomas (GBMs) are heterogeneous, treatment-resistant tumors that are driven by populations of cancer stem cells (CSCs). In this study, we perform an epigenetic-focused functional genomics screen in GBM organoids and identify WDR5 as an essential epigenetic regulator in the SOX2-enriched, therapy resistant cancer stem cell niche. METHODS/STUDY POPULATION: Despite their importance for tumor growth, few molecular mechanisms critical for CSC population maintenance have been exploited for therapeutic development. We developed a spatially resolved loss-of-function screen in GBM patient-derived organoids to identify essential epigenetic regulators in the SOX2-enriched, therapy resistant niche. Our niche-specific screens identified WDR5, an H3K4 histone methyltransferase responsible for activating specific gene expression, as indispensable for GBM CSC growth and survival. RESULTS/ANTICIPATED RESULTS: In GBM CSC models, WDR5 inhibitors blocked WRAD complex assembly and reduced H3K4 trimethylation and expression of genes involved in CSC-relevant oncogenic pathways. H3K4me3 peaks lost with WDR5 inhibitor treatment occurred disproportionally on POU transcription factor motifs, required for stem cell maintenance and including the POU5F1(OCT4)::SOX2 motif. We incorporated a SOX2/OCT4 motif driven GFP reporter system into our CSC cell models and found that WDR5 inhibitor treatment resulted in dose-dependent silencing of stem cell reporter activity. Further, WDR5 inhibitor treatment altered the stem cell state, disrupting CSC in vitro growth and self-renewal as well as in vivo tumor growth. DISCUSSION/SIGNIFICANCE: Our results unveiled the role of WDR5 in maintaining the CSC state in GBM and provide a rationale for therapeutic development of WDR5 inhibitors for GBM and other advanced cancers. This conceptual and experimental framework can be applied to many cancers, and can unmask unique microenvironmental biology and rationally designed combination therapies.

Under the recurring headline ‘the Concertina's Deadly Work in the Trenches’, several British newspapers reported in early 1900 that, during the ongoing siege of Mafeking, British army concertina players were capturing enemy soldiers by simply playing strains of the concertina to distract them out of their hiding places. ‘One is sorry to learn that the art of music should be pressed into service to lure persons to destruction’, a commentator in the Musical News noted, but then, it was rationalized, ‘all's fair in war’. This hybrid use of the concertina during the South African War was further employed as a metaphor for the decay of the physical body itself: as has been noted by Elizabeth van Heyningen, food in Boer concentration camps was so meagre that the meat served to prisoners was once described as coming from a ‘carcase [who] looks like a concertina drawn out fully with all the wind knocked out’. Likewise, Krebs (1999) has discussed the presence of the concertina in the trenches as an example of contemporaneous stereotypes about the susceptibility of Boer soldiers to music in relation to perceived notions that they were backwards and easily manipulated. Drawing upon references to music – particularly the ubiquitous, anthropomorphised, instrument of the concertina – in concentration camps during the South African War, this paper will situate the use of British military music at the dawn of the twentieth century within the framework of trauma studies, proposing that the soundscapes of imperial war were implicitly tinged with traces of physical suffering.

Wildfires have become a regular seasonal disaster across the Western region of the United States. Wildfires require a multifaceted disaster response. In addition to fire suppression, there are public health and medical needs for responders and the general population in the path of the fire, as well as a much larger population impacted by smoke. This paper describes key aspects of the health and medical response to wildfires in California, including facility evacuation and shelter medical support, with emphasis on the organization, coordination, and management of medical teams deployed to fire incident base camps. This provides 1 model of medical support and references resources to help other jurisdictions that must respond to the rising incidence of large wildland fires.

Background: Antibiotics are not recommended but are often prescribed for upper respiratory-tract infections (URIs). Prescribers cite patient expectation as a driver of inappropriate antibiotic prescribing; prior literature has demonstrated higher satisfaction scores in patients who receive antibiotics compared to those who do not. We assessed whether veteran satisfaction at URI visits was associated with antibiotic receipt or with reported expectation for antibiotics. Methods: We surveyed veterans with documented URI encounters in the Veterans’ Affairs Tennessee Valley Healthcare System between January 1, 2018, and December 31, 2019. Patients not evaluated in person, with documented dementia, or who died prior to the study start date were excluded. Veterans were asked to recall their URI visit and to complete the Patient Safety Questionnaire (PSQ)-18 (Rand Corporation) and questions assessing antibiotic expectations. The PSQ-18, an 18-item survey that assesses patient satisfaction, uses a 5-point Likert scale (ie, strongly disagree, disagree, uncertain, agree, strongly agree), yielding a composite score of 18–90. Higher scores represent more satisfaction with care. Demographic and visit-specific information were extracted via chart review. We used multivariable linear regression to assess differences in composite PSQ-18 satisfaction scores between those who did and did not receive an antibiotic, adjusted for patient and visit characteristics, and to assess differences in satisfaction scores for those who did and did not report expecting antibiotics, adjusted for antibiotic receipt. Results: We identified 1,435 patients seen for URI at 17 sites. After exclusions, 1,343 veterans were eligible for chart abstraction. After excluding 42 responders who responded after study close or returned blank surveys, the final analytic cohort included 432 (32.2%) of 1,343 responders; 225 (52.1%) received an antibiotic and 207 (47.9%) did not. Mean total satisfaction for veterans who received an antibiotic was 67.8 (SD, ±9.4) compared to 66.7 (SD, ±9.7) for those who did not (Figure 1). Increased total satisfaction was not significantly associated with antibiotic receipt (0.65; 95% CI, −2.0 to 3.3). Most veterans (72.0%) disagreed that visit satisfaction depended on antibiotic receipt. However, only 30.8% reported that they would not expect an antibiotic for URI visits. A significant reduction in total satisfaction (−4.1; 95% CI, −6.3 to −1.9) was associated with expecting compared to not expecting an antibiotic. Conclusions: Our findings suggest that prescribing an antibiotic is not associated with increased veteran satisfaction for URI visits but is associated with expecting an antibiotic. Future work will evaluate methods to change veteran antibiotic expectations.

Funding: No

Disclosures: None

Figure 1.

This research note reports on a new effort to track candidate diversity in Canadian elections. The dataset covers 4,516 candidates who ran in the 2008, 2011, 2015 and 2019 federal elections and includes novel data on their race, Indigenous background and age, alongside information on gender, occupation, prior electoral experience and electoral outcome. We outline the process for collecting and systematizing these data, which relied on genealogical methods and the principle of triangulation. The data can be used to track diversity among electoral candidates over time or merged with other sources to answer district-level questions about representational diversity, electoral dynamics, vote choice and political communications. The method could also be replicated and applied to other levels of government.