Background: Brain-specific glial fibrillary acidic protein (GFAP) can discriminate stroke type [ischemic stroke (AIS), intracerebral hemorrhage (ICH), stroke mimics (SM)]. Novel point-of-care technology (GFAP levels <15 minutes) is a promising diagnostic tool. We aim to evaluate the feasibility of rapid GFAP analysis in acute stroke. Methods: Exploratory analysis of an ongoing prospective study of suspected undifferentiated stroke <24h from onset. Rapid plasma GFAP levels (pg/mL) are measured at hospital arrival using the i-STAT Alinity® instrument and commercially-available cartridges. Study endpoints include quantitative GFAP levels according to final diagnosis and time from stroke onset. Results: Among 200 patients (mean(±SD) 70.7±15.5 years, 44.5% female, median (IQR) NIHSS 9(4-19), diagnosis was AIS (n=132 (59 large-vessel occlusion), ICH (n=17), and SM (n=51). Median time from hospital arrival to GFAP result was 56.0 (47.0-69.5) minutes. Median rapid GFAP levels were highest in ICH (878.0 (70.5-3,906.5) pg/mL) compared to AIS (49.5 (29.0-95)pg/mL) and SM (29(29-64)pg/mL), p=0.001. Median GFAP was higher in AIS-known onset >4.5h (n=9) (110.0 (44.0-216.0) pg/mL) compared to AIS<4.5h (40.5 (29.0-68.8) pg/mL) (n=72), (p=0.047), while AIS-unknown onset (n=51) (68.0 (29.0-108.5) pg/mL) fell between these two groups, likely reflecting the subgroup’s heterogeneity. Conclusions: Preliminary findings suggest that rapid GFAP analysis is feasible in acute stroke and may inform treatment decisions.

Multicenter clinical trials are essential for evaluating interventions but often face significant challenges in study design, site coordination, participant recruitment, and regulatory compliance. To address these issues, the National Institutes of Health’s National Center for Advancing Translational Sciences established the Trial Innovation Network (TIN). The TIN offers a scientific consultation process, providing access to clinical trial and disease experts who provide input and recommendations throughout the trial’s duration, at no cost to investigators. This approach aims to improve trial design, accelerate implementation, foster interdisciplinary teamwork, and spur innovations that enhance multicenter trial quality and efficiency. The TIN leverages resources of the Clinical and Translational Science Awards (CTSA) program, complementing local capabilities at the investigator’s institution. The Initial Consultation process focuses on the study’s scientific premise, design, site development, recruitment and retention strategies, funding feasibility, and other support areas. As of 6/1/2024, the TIN has provided 431 Initial Consultations to increase efficiency and accelerate trial implementation by delivering customized support and tailored recommendations. Across a range of clinical trials, the TIN has developed standardized, streamlined, and adaptable processes. We describe these processes, provide operational metrics, and include a set of lessons learned for consideration by other trial support and innovation networks.

Grazing is a crucial component of dairy farms across many regions of the world. This review explores challenges related to grazing infrastructure and opportunities for future improvement. Farmers who aim to increase pasture utilisation face heightened inter-animal competition necessitated by pasture restriction to achieve target post-grazing sward heights. Increasing the frequency of fresh pasture allocation beyond once per day has been observed to reduce milk production in primiparous animals, due to intensified competition for limited feed resources. Implementing grazing paddocks tailored for 24- to 36-hour allocations helps to mitigate inter-animal competition while concurrently preventing the grazing of fresh regrowth. Crucial to this approach is establishing farm roadway infrastructure that allows access to all sections of the grazing platform. However, the development of these roadway networks has often occurred without a comprehensive assessment of their impact on the efficiency of the dairy herd’s movement between grazing paddocks and the milking parlour. The efficiency of the dairy herd’s movement is most significantly influenced by the location of the milking parlour within the grazing platform. Extreme walking distances or challenging terrain on farm roadways may have an impact on milk production per cow. Factors such as farm roadway surface quality and width significantly influence cow throughput on farm roadways. Recent studies have highlighted inadequate roadway widths on many farms relative to their herd size, while surface condition may also be limiting cow throughput on these farms. Enhancing roadway width and surface condition of farm roadways may improve labour efficiency on commercial farms.

In 2015, a continuous 15.4 m snow/firn core was recovered from central South Georgia Island at ∼850 m a.s.l. All firn core samples were analyzed for major (Al, Ca, Mg, Na, K, Ti and Fe) and trace element concentrations (Sr, Cd, Cs, Ba, La, Ce, Pr, Pb, Bi, U, As, Li, S, V, Cr, Mn, Co, Cu and Zn) and stable water isotopes. The chemical and isotopic signal is well preserved in the top 6.2 m of the core. Below this depth, down to the bottom of the core, signal dampening is observed in the majority of the elemental species making it difficult to distinguish a seasonal signal. Thirteen elements (As, Bi, Ca, Cd, Cu, K, Li, Mg, Na, Pb, S, Sr and Zn) have crustal enrichment factor values higher than 10 suggesting sources in addition to those found naturally in the crust. While this study shows that 850 m a.s.l. is not high enough to preserve a record including recent years, higher-elevation (>1250 m a.s.l.) glaciers may be likely candidates for ice core drilling to recover better-preserved, continuous, recent to past glaciochemical records.

Highly portable and accessible MRI technology will allow researchers to conduct field-based MRI research in community settings. Previous guidance for researchers working with fixed MRI does not address the novel ethical, legal, and societal issues (ELSI) of portable MRI (pMRI). Our interdisciplinary Working Group (WG) previously identified 15 core ELSI challenges associated with pMRI research and recommended solutions. In this article, we distill those detailed recommendations into a Portable MRI Research ELSI Checklist that offers practical operational guidance for researchers contemplating using this technology.

Historical changes from shifting land use, the natural meandering of waterways, and the aftereffects of erosion complicate modern environments and obfuscate precontact landscapes. Although archaeologists can create stratified sampling models or employ systematic surveys, traditional field methodologies are often not suitable for site discovery, thereby limiting knowledge of ancient cultural landscapes. Many water systems in southern Louisiana, and in many parts of the world, have been covered or concealed in backswamps by natural geomorphological processes, development, or environmental degradation. Investigation standards that do not account for these changes will not be effective at identifying archaeological sites in such transformed landscapes. Discoveries made during ongoing archaeological research in Iberville Parish, Louisiana, provide examples of what can be missed and offer solutions through changes in archaeological field methods. This article advocates for a mixed-methodology approach, drawing from historical research and shallow geophysics to look at landforms and landscape changes. Strictly following state survey guidelines can muddle the archaeological record, particularly in places subject to significant landscape change from historical land-use alteration. By applying these approaches, we offer a way to reconstruct ancient landscapes and landforms that are culturally significant but often missed given the nature of modern environmental conditions.

The negative predictive value of blaCTX-M on BCID2 for ceftriaxone resistance in E. coli and K. pneumoniae group was 97% and 94%, respectively. Creation of a genotypic antibiogram led to updated local guidance for clinicians to utilize for empiric treatment of Enterobacterales bloodstream infections identified via rapid diagnostics.

Congenital coronary artery stenosis coexisting with aortic coarctation in nonsyndromic patients has not previously been reported. This report describes a nonsyndromic aortic coarctation patient who experienced intraoperative cardiac arrest due to a previously undiagnosed critical left main coronary artery stenosis. The patient was successfully resuscitated, underwent patch coronary ostioplasty, and was discharged home. He remains well for four months following repair.

Background: Neurodegeneration with Brain Iron Accumulation (NBIA) is a heterogenous group of disorders with the common theme of iron accumulation in the basal ganglia. These disorders typically present in childhood with progressive neurodegeneration and neuropsychiatric symptoms. Caring for an individual with NBIAs is intensive, however it is unknown what factors impact caregiver well-being and quality of life. Methods: Common themes were obtained via literature review of quality of life surveys in children with neurological and chronic illnesses. Five domains were addressed: Diagnosis, Communication, Symptom Management, Clinical Experience and Resources/Support. The survey was approved by the Family Advisory Committee at the CHEO Research Institute and the CHEO REB. The survey was distributed via the Rare Connect Platform to Canadian caregivers. Results: Survey responses are being analyzed and will be presented at the CNSF. Within each domain, Likert scales will be analyzed. Domains will be ranked according to the caregiver responses. Conclusions: Results of this survey will assist in developing care management guidelines, resources for families and help with future advocacy for patients and families affected by NBIAs. The results will also help guide future NBIA Canada Family Conferences.

Illite diagenesis in Tertiary and Mesozoic shales in the Bergen High area, northern North Sea, was studied using mineralogic, isotopic, and computerized thermal modeling techniques. The Tertiary shales are dominated by smectite, with lesser amounts of illite, kaolinite, and chlorite. At present burial temperatures of >70°C smectite is absent, and the shales contain abundant lath-shaped illite which yields a mixed-layer illite/smectite (I/S) X-ray powder diffraction (XRD) pattern. Transmission electron microscopy (TEM) indicates that the illite laths increase in abundance and thickness with increasing depth; XRD patterns indicate a progressive increase in the illite component of the I/S. The deepest samples were found to contain long-range ordered (R=3) I/S, which showed platy particle morphology with the TEM. K-Ar ages of most of the <0.1-μm-size illite separates imply that illitization was a relatively brief event affecting a thick sequence of sediments during late Cretaceous to early Paleocene time (65–87 Ma); however, measured ages were affected by trace levels of detrital Ar contamination and do not represent the true age of diagenesis.

Several methods of quantifying Ar contamination were used to correct measured ages to obtain a reasonable estimate of the true age of diagenesis. The corrected ages are imprecise due to uncertainties in quantifying the levels of sample contamination, but generally suggest a Paleogene (38–66 Ma) period of illitization. In contrast, simple kinetic models of smectite-illitization suggest much younger ages of diagenesis (0–40 Ma at the Veslefrikk field; 0–60 Ma at the Huldra field). The timing of the diagenesis and the morphologic aspects of the authigenic illite suggest that illite precipitated before late Tertiary compaction and resulted in a decrease in fluid permeability. Low trapping efficiency of early Tertiary sediments, vertical escape of warm fluid from the Brent sandstone, and high heat flow may have promoted illite diagenesis in the shales prior to deep late-Tertiary burial.

The Eocene Huber Formation, exposed in the CFI Hall mine south of Irwinton, in Wilkinson County, Georgia, displays colored zones that are a consequence of an oxidative weathering front. These zones vary from very light gray (gray) to pinkish white (pink) to very pale yellow (cream) (Munsell N8, 5YR 8/2 and 2.5Y 8/2, respectively) with increasing degree of oxidation. A representative sample from each zone was collected and analyzed for its chemical, crystallographic and Raman spectroscopic properties. The comparison of these genetically related samples allowed for a quantitatively accurate assessment of anatase’s contribution to the total TiO2 content. All samples contain ∼1.5 wt.% TiO2. Progressing from gray to pink to cream, anatase contents range from half to nearly all the TiO2. The a lattice parameter for anatase is constant in all three kaolins (3.7916 Å), suggesting a constant 4.6 mol.% isomorphous substitution of Fe for Ti.

Phase characterization and mass-balance considerations of the gray, pink and cream kaolins indicate that ilmenite and pyrite are precursor Ti- and Fe-bearing phases in the gray kaolin that undergo oxidation. Pseudorutile is a proposed intermediate phase resulting from the anodic corrosion of ilmenite. Hematite, goethite and anatase are the dominant end-products via dissolution-precipitation from the reactants pyrite and pseudorutile. The removal of Fe sulfides and organic matter and addition of hematite and goethite causes kaolin colors to change from gray to pink. Oxygen diffusing from groundwater in permeable overlying and underlying formations facilitates a process that probably involves aerobic and anaerobic bacteria that utilize Fe from pyrite, hematite and goethite. We postulate that the end result is the mobilization of Fe by siderophores and a kaolin color change from pink to cream.

Strong gas-mineral interactions or slow adsorption kinetics require a molecular-level understanding of both adsorption and diffusion for these interactions to be properly described in transport models. In this combined molecular simulation and experimental study, noble gas adsorption and mobility is investigated in two naturally abundant zeolites whose pores are similar in size (clinoptilolite) and greater than (mordenite) the gas diameters. Simulated adsorption isotherms obtained from grand canonical Monte Carlo simulations indicate that both zeolites can accommodate even the largest gas (Rn). However, gas mobility in clinoptilolite is significantly hindered at pore-limiting window sites, as seen from molecular dynamics simulations in both bulk and slab zeolite models. Experimental gas adsorption isotherms for clinoptilolite confirm the presence of a kinetic barrier to Xe uptake, resulting in the unusual property of reverse Kr/Xe selectivity. Finally, a kinetic model is used to fit the simulated gas loading profiles, allowing a comparison of trends in gas diffusivity in the zeolite pores.