We investigated the effects of maternal vitamin and mineral supplementation throughout gestation on gene expression in the jejunal mucosa of neonatal calves. Crossbred Angus heifers (n = 14) were estrus synchronized, bred to female-sexed semen, and randomly assigned to a basal diet (Control, CON; n = 7) or the basal diet plus vitamin and mineral supplement (Treatment, VTM; n = 7). After parturition, calves were removed from their dams before suckling, fed colostrum replacer, and euthanized 30 h after the first feeding. A subsample of the mucosa of the mid-jejunum was collected, and total RNA was isolated. Gene expression was measured using RNA-Seq, and differentially expressed genes (DEGs) were identified using DESeq2. We identified 528 DEGs from the jejunal mucosa between the VTM and CON calves (P ≤ 0.05 and |log2FC| ≥ 0.5). The DEGs were associated with nutrient transport, lipid metabolism, and immune-related biological processes and pathways. Interestingly, genes underlying the complement and coagulation cascades were mostly downregulated in calves born to VTM dams. On the other hand, the cytokine-cytokine receptor interaction KEGG pathway showed most genes upregulated (LIFR, KDR, TNFRSF4, TNFSF18, FLT1, and TNFRSF12A). Our results show that vitamin and mineral supplementation throughout gestation affects genes underlying tissue structure, nutrient transport and metabolism, and immune system pathways in neonates. The implications of such changes and the long-term outcomes on herd health and performance warrant further research.

Tetflupyrolimet (Dodhylex™ Active, FMC Corporation) is a novel herbicide inhibiting de novo pyrimidine biosynthesis that controls grassy weeds preemergence in rice (Oryza sativa L.) production. Field trials were conducted from 2021 to 2024 to evaluate turfgrass tolerance to tetflupyrolimet applications for annual bluegrass (Poa annua L.) and smooth crabgrass [Digitaria ischaemum (Schreb.) Schreb. ex Muhl.] control. Tolerance was evaluated on seven turfgrass species, including creeping bentgrass (Agrostis stolonifera L.), Kentucky bluegrass (Poa pratensis L.), tall fescue [Schedonorus arundinaceus (Schreb.) Dumort.; syn.: Festuca arundinacea Schreb.], hybrid bermudagrass [Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt-Davy], and manilagrass [Zoysia matrella (L.) Merr.] at various mowing heights ranging from 3.8 to 12.5 mm. Separate experiments were conducted on each turfgrass species to evaluate tolerance in both fall and spring. Tetflupyrolimet was applied at rates of 0, 25, 50, 100, 200, 400, 800, 1600, 3200, or 6400 g ai ha−1. No injury was observed on any warm-season turfgrass species in either season, whereas cool-season grass tolerance varied among species each season; however, cool-season turfgrass tolerance for all species was greater in spring than fall. While efficacy of tetflupyrolimet (400 g ha−1) for preemergence D. ischaemum control varied among years, mixtures of tetflupyrolimet (400 g ha−1), pyroxasulfone (128 g ai ha−1), and rimsulfuron (35 g ai ha−1) applied preemergence or early postemergence effectively controlled multiple-resistant P. annua in both seasons. Overall, these findings highlight that warm-season turfgrasses are highly tolerant of tetflupyrolimet applications for P. annua or D. ischaemum control.

Fetal growth restriction (FGR) is associated with increased risk of developing non-communicable diseases. We have a placenta-specific nanoparticle gene therapy protocol that increases placental expression of human insulin-like growth factor 1 (hIGF1), for the treatment of FGR in utero. We aimed to characterize the effects of FGR on hepatic gluconeogenesis pathways during early stages of FGR establishment, and determine whether placental nanoparticle-mediated hIGF1 therapy treatment could resolve differences in the FGR fetus. Female Hartley guinea pigs (dams) were fed either a Control or Maternal Nutrient Restriction (MNR) diet using established protocols. At GD30-33, dams underwent ultrasound guided, transcutaneous, intraplacental injection of hIGF1 nanoparticle or PBS (sham) and were sacrificed 5 days post-injection. Fetal liver tissue was fixed and snap frozen for morphology and gene expression analysis. In female and male fetuses, liver weight as a percentage of body weight was reduced by MNR, and not changed with hIGF1 nanoparticle treatment. In female fetal livers, expression of hypoxia inducible factor 1 (Hif1α) and tumor necrosis factor (Tnfα) were increased in MNR compared to Control, but reduced in MNR + hIGF1 compared to MNR. In male fetal liver, MNR increased expression of Igf1 and decreased expression of Igf2 compared to Control. Igf1 and Igf2 expression was restored to Control levels in the MNR + hIGF1 group. This data provides further insight into the sex-specific mechanistic adaptations seen in FGR fetuses and demonstrates that disruption to fetal developmental mechanisms may be returned to normal by treatment of the placenta.

Major depressive disorder (MDD) is a common, debilitating, phenotypically heterogeneous disorder with heritability ranges from 30% to 50%. Compared to other psychiatric disorders, its high prevalence, moderate heritability, and strong polygenicity have posed major challenges for gene-mapping in MDD. Studies of common genetic variation in MDD, driven by large international collaborations such as the Psychiatric Genomics Consortium, have confirmed the highly polygenic nature of the disorder and implicated over 100 genetic risk loci to date. Rare copy number variants associated with MDD risk were also recently identified. The goal of this review is to present a broad picture of our current understanding of the epidemiology, genetic epidemiology, molecular genetics, and gene–environment interplay in MDD. Insights into the impact of genetic factors on the aetiology of this complex disorder hold great promise for improving clinical care.

Optimising oilseed rape canopy size through correct management is crucial for maximising yield. Plant growth regulators (PGRs) and nitrogen (N) fertiliser are generally applied at a flat rate, however variable applications may be useful for the optimisation of canopy size. The aim of this paper was to understand the potential for spectral reflectance indices to predict green area index (GAI) and crop N content in winter oilseed rape, with specific focus on the Fritzmeier Isaria Crop Sensor. Three large oilseed rape chessboard experiments were set up in 2015 and 2016 in the UK. The results show good correlations between the Isaria indices and both GAI and crop N content, suggesting that the Isaria may be a useful tool for variably applying PGRs and N fertiliser to oilseed rape.

A range of precision farming technologies are used commercially for variable rate applications of nitrogen (N) for cereals, yet these usually adjust N rates from a pre-set value, rather than predicting economically optimal N requirements on an absolute basis. This paper reports chessboard experiments set up to examine variation in N requirements, and to develop and test systems for its prediction, and to assess its predictability. Results showed very substantial variability in fertiliser N requirements within fields, typically >150 kg ha−1, and large variation in optimal yields, typically >2 t ha−1. Despite this, calculated increases in yield and gross margin with N requirements perfectly matched across fields were surprisingly modest (compared to the uniform average rate). Implications are discussed, including the causes of the large remaining variation in grain yield, after N limitations were removed.

Cryptosporidium, a parasite known to cause large drinking and recreational water outbreaks, is tolerant of chlorine concentrations used for drinking water treatment. Human laboratory-based surveillance for enteric pathogens detected a cryptosporidiosis outbreak in Baker City, Oregon during July 2013 associated with municipal drinking water. Objectives of the investigation were to confirm the outbreak source and assess outbreak extent. The watershed was inspected and city water was tested for contamination. To determine the community attack rate, a standardized questionnaire was administered to randomly sampled households. Weighted attack rates and confidence intervals (CIs) were calculated. Water samples tested positive for Cryptosporidium species; a Cryptosporidium parvum subtype common in cattle was detected in human stool specimens. Cattle were observed grazing along watershed borders; cattle faeces were observed within watershed barriers. The city water treatment facility chlorinated, but did not filter, water. The community attack rate was 28·3% (95% CI 22·1–33·6), sickening an estimated 2780 persons. Watershed contamination by cattle probably caused this outbreak; water treatments effective against Cryptosporidium were not in place. This outbreak highlights vulnerability of drinking water systems to pathogen contamination and underscores the need for communities to invest in system improvements to maintain multiple barriers to drinking water contamination.

Although rare, typhoid fever cases acquired in the United States continue to be reported. Detection and investigation of outbreaks in these domestically acquired cases offer opportunities to identify chronic carriers. We searched surveillance and laboratory databases for domestically acquired typhoid fever cases, used a space–time scan statistic to identify clusters, and classified clusters as outbreaks or non-outbreaks. From 1999 to 2010, domestically acquired cases accounted for 18% of 3373 reported typhoid fever cases; their isolates were less often multidrug-resistant (2% vs. 15%) compared to isolates from travel-associated cases. We identified 28 outbreaks and two possible outbreaks within 45 space–time clusters of ⩾2 domestically acquired cases, including three outbreaks involving ⩾2 molecular subtypes. The approach detected seven of the ten outbreaks published in the literature or reported to CDC. Although this approach did not definitively identify any previously unrecognized outbreaks, it showed the potential to detect outbreaks of typhoid fever that may escape detection by routine analysis of surveillance data. Sixteen outbreaks had been linked to a carrier. Every case of typhoid fever acquired in a non-endemic country warrants thorough investigation. Space–time scan statistics, together with shoe-leather epidemiology and molecular subtyping, may improve outbreak detection.

This paper describes the system architecture of a newly constructed radio telescope – the Boolardy engineering test array, which is a prototype of the Australian square kilometre array pathfinder telescope. Phased array feed technology is used to form multiple simultaneous beams per antenna, providing astronomers with unprecedented survey speed. The test array described here is a six-antenna interferometer, fitted with prototype signal processing hardware capable of forming at least nine dual-polarisation beams simultaneously, allowing several square degrees to be imaged in a single pointed observation. The main purpose of the test array is to develop beamforming and wide-field calibration methods for use with the full telescope, but it will also be capable of limited early science demonstrations.

Dr. Loran B. Smith passed away in Topeka, Kansas, on July 24, 2009. He was born on July 23, 1946. He was the son of Gordon T and Edith A (Hibbard) Smith of Medford, Massachusetts. Loran received his bachelors degree at Salem State College (Massachusetts) in 1968, a masters from Oklahoma State in 1971, and then taught at Black Hills State (Spearfish, South Dakota) from 1971–1974 and Augustana College in Souix Falls from 1974–1977. He received his Ph.D. from the University of Nebraska-Lincoln in 1980 and taught at Missouri Southern State College in Joplin until 1982. He then came to Washburn University of Topeka, where he taught until his death. While “Doc” Smith (as the students referred to him) published sufficiently enough to be awarded tenure and promotion to professor, that was not his forte. Loran was a gifted teacher. His CV lists 23 teaching awards, including Washburn's Faculty Certificate of Merit, a university-wide teaching honor based on student elections, from 1985–1998. Loran was also extremely active in faculty governance and other service to the university and the Topeka community. He was on the university's faculty governing body from 1996–2006, serving as its vice president in 2002 and president from 2003–2005. He was the chairman of the Social Science Division almost all of the 1990s and he also served as the chairman of the college's curriculum committee during that same time span. As Washburn is an open-admission university, we have retention problems not experienced by most universities. Loran researched, organized, and ran a college experience program for at-risk students. He was very active in ASPA, serving as the Kansas chapter president from 1987–1988, indeed, his auto license plate read “KS ASPA” and was purchased for him by students he had recruited into ASPA. Loran's main area of academic interest was state and local government and he was the election night expert for one of the local TV stations here in the capital of Kansas from 1984–1992. What occupied most of his time and energy outside of his official academic duties was serving as the faculty advisor for a local chapter of the Sigma Phi Epsilon fraternity. Doc Smith took what was a typical college fraternity and turned it into a modern association of men that consistently had the highest average GPA of all the fraternities and sororities on campus. It was not unusual for Loran to pay for a student's tuition and fraternity house bill, buy students books, and lend money to a needy student. Loran had a reputation for frugality (his apartment had a TV but no cable, a rotary phone, and he rented all of his furniture and appliances). Loran's tightness with money turned out to be a big benefit for the fraternity. One chapter official put it this way, “Through his notorious tight-fisted watch over finances, the Chapter was able to wipe out a significant debt to the National Housing Corporation ahead of schedule and helped the chapter build a significant savings by 2000.” People who knew Loran thought that he was not married but Loran was married to his job. Not only was Loran in his office nearly every evening until 10:00 p.m., but he was there all day Saturday and Sunday too, and, more often than not, there was a student in that office talking with him.

Common mental health problems are highly prevalent in primary care, the UK National Service Framework for mental health demanding that effective and accessible services be made available. Although built upon a strong evidence base, traditional psychological therapies are often limited in terms of their applicability and availability. As a consequence innovative self-help programmes are increasingly being advocated as an alternative means of managing mental health illness within primary care. This study reports the results of a three month evaluation of a self-help service provided by a busy UK urban Primary Care Trust. Levels of utilization, effectiveness and stakeholder acceptability were examined through a combination of quantitative and qualitative data. A total of 662 patients were referred to the self-help clinics over a three month period, 67% of whom attended their first appointment. The mean number of sessions per patient was 2.8 (SD = 2.4), with an average total time of 69.6 min (SD = 48.2). Mean Clinical Outcomes in Routine Evaluation (CORE-OM) scores improved significantly between baseline and three month follow-up (P < 0.001), 39% of patients demonstrating a clinically significant improvement. Both selfhelp therapists and referring general practitioners reported moderate to high satisfaction with the self-help treatment model, with the majority of patients perceiving the intervention to be appropriate to their needs. Data demonstrated that, whilst there was a clear need for a simple self-help service to be based in primary care, the ultimate success of this provision necessitates a well developed infrastructure capable of providing sufficient support and information to ensure that it is flexible and responsive to individual needs.