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Understanding the factors contributing to optimal cognitive function throughout the aging process is essential to better understand successful cognitive aging. Processing speed is an age sensitive cognitive domain that usually declines early in the aging process; however, this cognitive skill is essential for other cognitive tasks and everyday functioning. Evaluating brain network interactions in cognitively healthy older adults can help us understand how brain characteristics variations affect cognitive functioning. Functional connections among groups of brain areas give insight into the brain’s organization, and the cognitive effects of aging may relate to this large-scale organization. To follow-up on our prior work, we sought to replicate our findings regarding network segregation’s relationship with processing speed. In order to address possible influences of node location or network membership we replicated the analysis across 4 different node sets.
Participants and Methods:
Data were acquired as part of a multi-center study of 85+ cognitively normal individuals, the McKnight Brain Aging Registry (MBAR). For this analysis, we included 146 community-dwelling, cognitively unimpaired older adults, ages 85-99, who had undergone structural and BOLD resting state MRI scans and a battery of neuropsychological tests. Exploratory factor analysis identified the processing speed factor of interest. We preprocessed BOLD scans using fmriprep, Ciftify, and XCPEngine algorithms. We used 4 different sets of connectivity-based parcellation: 1)MBAR data used to define nodes and Power (2011) atlas used to determine node network membership, 2) Younger adults data used to define nodes (Chan 2014) and Power (2011) atlas used to determine node network membership, 3) Older adults data from a different study (Han 2018) used to define nodes and Power (2011) atlas used to determine node network membership, and 4) MBAR data used to define nodes and MBAR data based community detection used to determine node network membership.
Segregation (balance of within-network and between-network connections) was measured within the association system and three wellcharacterized networks: Default Mode Network (DMN), Cingulo-Opercular Network (CON), and Fronto-Parietal Network (FPN). Correlation between processing speed and association system and networks was performed for all 4 node sets.
Results:
We replicated prior work and found the segregation of both the cortical association system, the segregation of FPN and DMN had a consistent relationship with processing speed across all node sets (association system range of correlations: r=.294 to .342, FPN: r=.254 to .272, DMN: r=.263 to .273). Additionally, compared to parcellations created with older adults, the parcellation created based on younger individuals showed attenuated and less robust findings as those with older adults (association system r=.263, FPN r=.255, DMN r=.263).
Conclusions:
This study shows that network segregation of the oldest-old brain is closely linked with processing speed and this relationship is replicable across different node sets created with varied datasets. This work adds to the growing body of knowledge about age-related dedifferentiation by demonstrating replicability and consistency of the finding that as essential cognitive skill, processing speed, is associated with differentiated functional networks even in very old individuals experiencing successful cognitive aging.
The glaciogenic nature of the Yudnamutana Subgroup was first recognized over a century ago, and its global significance was recognized shortly after, with the eventual postulation of a global Sturtian Glaciation and Snowball Earth theory. Much debate on the origin and timing of these rocks, locally and globally, has ensued in the years since. A significant corpus of research on the lithology, sedimentology, geochronology and formal lithostratigraphy of these sequences globally has attempted to resolve many of these debates. In the type area for the Sturtian Glaciation, South Australia’s Adelaide Superbasin, the lithostratigraphy and sedimentology are well understood; however, formal stratigraphic nomenclature has remained complicated and contested. Absolute dates on the stratigraphy are also extremely sparse in this area. The result of these longstanding issues has been disagreement as to whether the sedimentary rocks of the Yudnamutana Subgroup are truly correlative throughout South Australia, and if they were deposited in the same time span recently defined for Sturtian glacial rocks globally, c. 717 Ma to c. 660 Ma. This study presents a large detrital zircon study, summarizes and compiles existing global geochronology for the Sturtian Glaciation and revises the formal lithostratigraphic framework of the Yudnamutana Subgroup. We show equivalence of the rocks that comprise the revised Sturt Formation, the main glaciogenic unit of the Yudnamutana Subgroup, and that it was deposited within the time span globally defined for the Sturtian Glaciation.
This study was designed to investigate the physiological responses induced in sheep (n = 18) by two different loading techniques followed by a short road journey. All animals were prepared with venous catheters, to minimize the disturbing effects of blood sampling, and nine sheep were fitted with heart rate monitors. The animals were loaded onto a transport vehicle in groups of three, alternately using a conventional tailgate ramp or a crate raised with a hydraulic lift. When all of the sheep were loaded, they were taken on a journey lasting 195min. Blood samples were collected in the home pen, directly after loading, and at 15min intervals during the journey. Measurements were made of plasma concentrations of Cortisol, prolactin and catecholamines (adrenaline and noradrenaline). The results indicated that heart rate increased during loading, regardless of the method used. No changes in concentrations of Cortisol or the catecholamines were detected, although a small increase in prolactin was noted when animals were loaded using the ramp. During transport, all sheep exhibited increases in plasma Cortisol concentrations which were greatest during the first 2h of the journey. The results suggest that, under the conditions employed in this experiment, the effects of the two loading procedures were similar and that transport appeared to be more stressful than loading.
For animals undergoing rehabilitation it is vital to monitor welfare in a way that is feasible, practical, and limits stress to the animal. The industry gold standard is to assess welfare under the Five Domains model, including nutrition, environment, physical health, and behaviour as the first four physical domains and mental domain as the fifth. Feasibility and effectiveness of these domains for assessing welfare of sea turtles undergoing rehabilitation were reviewed and it was determined that the mental state can be best assessed through behavioural changes. A scoping review of the literature was conducted using Scopus and Web of Science to investigate use of environmental enrichment devices (EEDs) as a measure of welfare in sea turtles. Behavioural assessments using EEDs were found to be well-documented; however, most EED studies pertained largely to livestock or zoo animals. Furthermore, studies rarely concentrated on reptiles, and specifically sea turtles. Results also showed that certain welfare assessment methods may be less appropriate for short-term captivity experienced during rehabilitation. Additionally, the hospital environment limits the ability to address some of the domains (ie biosecurity, feasibility, safety of turtle, etc, might be compromised). This review shows that only three of the nine environmental enrichment strategies described in the literature suit the specific requirements of sea turtles in rehabilitation: feeding, tactile, and structural. It is documented that turtles display behaviours that would benefit from EEDs and, therefore, more specific studies are needed to ensure the best welfare outcomes for sea turtles undergoing rehabilitation.
There is evidence that the COVID-19 pandemic has negatively affected mental health, but most studies have been conducted in the general population.
Aims
To identify factors associated with mental health during the COVID-19 pandemic in individuals with pre-existing mental illness.
Method
Participants (N = 2869, 78% women, ages 18–94 years) from a UK cohort (the National Centre for Mental Health) with a history of mental illness completed a cross-sectional online survey in June to August 2020. Mental health assessments were the GAD-7 (anxiety), PHQ-9 (depression) and WHO-5 (well-being) questionnaires, and a self-report question on whether their mental health had changed during the pandemic. Regressions examined associations between mental health outcomes and hypothesised risk factors. Secondary analyses examined associations between specific mental health diagnoses and mental health.
Results
A total of 60% of participants reported that mental health had worsened during the pandemic. Younger age, difficulty accessing mental health services, low income, income affected by COVID-19, worry about COVID-19, reduced sleep and increased alcohol/drug use were associated with increased depression and anxiety symptoms and reduced well-being. Feeling socially supported by friends/family/services was associated with better mental health and well-being. Participants with a history of anxiety, depression, post-traumatic stress disorder or eating disorder were more likely to report that mental health had worsened during the pandemic than individuals without a history of these diagnoses.
Conclusions
We identified factors associated with worse mental health during the COVID-19 pandemic in individuals with pre-existing mental illness, in addition to specific groups potentially at elevated risk of poor mental health during the pandemic.
This study seeks the opinions of qualified doctors on what they feel medical students should learn about otolaryngology. It aims to identify both the content deemed relevant and the performance levels for medical students in otolaryngology.
Methods
A national survey developed from a content analysis of undergraduate otolaryngology curricula from the UK was undertaken, accompanied by a review of the literature and input from an expert group. Data were collected from a wide range of doctors.
Results
Participants felt that graduating students should be able to: recognise, assess and initiate management for common and life-threatening acute conditions; take an appropriate patient history; and perform an appropriate examination for the majority of otolaryngology clinical conditions but manage only a select few.
Conclusion
This study reports performance levels for otolaryngology topics at an undergraduate level. Participating doctors felt that a higher level of performance should be expected of students treating life-threatening, acute and common otolaryngology conditions.
Efforts to move community engagement in research from marginalized to mainstream include the NIH requiring community engagement programs in all Clinical and Translational Science Awards (CTSAs). However, the COVID-19 pandemic has exposed how little these efforts have changed the dominant culture of clinical research. When faced with the urgent need to generate knowledge about prevention and treatment of the novel coronavirus, researchers largely neglected to involve community stakeholders early in the research process. This failure cannot be divorced from the broader context of systemic racism in the US that has contributed to Black, Indigenous, and People of Color (BIPOC) communities bearing a disproportionate toll from COVID-19, being underrepresented in COVID-19 clinical trials, and expressing greater hesitancy about COVID-19 vaccination. We call on research funders and research institutions to take decisive action to make community engagement obligatory, not optional, in all clinical and translational research and to center BIPOC communities in this process. Recommended actions include funding agencies requiring all research proposals involving human participants to include a community engagement plan, providing adequate funding to support ongoing community engagement, including community stakeholders in agency governance and proposal reviews, promoting racial and ethnic diversity in the research workforce, and making a course in community engaged research a requirement for Masters of Clinical Research curricula.
Media coverage of non-suicidal self-injury (NSSI) ranges from providing helpful education to displaying graphic images. We offer the first research-informed, consensus-based guidelines for the responsible reporting and depicting of NSSI in the media, while also advising on ideas for dissemination and collaboration between media professionals and healthcare experts.
Uraniferous particles from contaminated environmental samples were analysed by scanning electronmicroscopy with energy dispersive X-ray analysis (SEM-EDXA) and microfocus extended X-rayabsorption fine structure (mEXAFS) spectroscopy. The particles of interest are uranium oxides, whichwere released into the environment by the combustion of scrap depleted uranium (DU) metal at afactory in Colonie, New York, USA. Most of the identified particles appear to have primary, ‘asemitted’ morphologies; some have evidence of minor dissolution, including corrosion pitting.Polycrystalline and often hollow microscopic spheres were identified, which are similar to particlesproduced by DU munitions impacting armoured targets. They are attributed to the autothermicoxidation of melt droplets. The compositions of the analysed spheres are dominated by UO2+x withvariable amounts of U3O8, two of the least soluble and least bioaccessible phases of U. These particles,collected from dusts and soils, have survived more than 25 y in the terrestrial environment. This studyfurther supports the case for using Colonie as an analogue for battlefield DU contamination.
Knowledge of the solid-phase speciation of As in Bengali sediments associated with hazardous As-rich groundwaters is crucial to understanding the processes controlling As release. The local coordination environment of As in such a sediment has been probed using K-edge As EXAFS. This revealed that As exists predominantly in its oxidized form, As(V), probably adsorbed as bidentate arsenate tetrahedra on metal (Fe and/or Al) oxide/hydroxide surfaces, although incorporation of As into a metal oxide structure cannot be ruled out. Arsenic was found to occur in several different coordination environments and this, together with the low concentration (<10 μg g–1) of As in the sediment prevented the unambiguous assignment of the second coordination sphere. The EXAFS analysis of the sediment after incubation under anaerobic conditions in the presence of added electron donor for metal reduction indicated changes in the relative concentrations of different solid-phase As species, providing circumstantial evidence for differential susceptibility to microbial action.
Arsenic mobilization and Fe(III) reduction in acetate-amended sediments collected from a range of depths from an aquifer with elevated groundwater arsenic concentrations in West Bengal were monitored over a 1 month period. Significant arsenic release was noted in sediment collected from 24 m and 45 m depth, with some Fe(III) reduction also observed in the 24 m sample. The structure of the microbial communities present in the sediments prior to incubation showed marked differences down the sediment column. Profiling of the microbial community in the 24 m and 45 m samples revealed a relatively complex make-up, with Acinetobacter species comprising the bulk of the 24 m sedimentary bacterial population, but no previously characterized As(V)-reducers were detected in either sample.
Previous studies from our laboratory have suggested a role for indigenous metal-reducing bacteria in the reduction of sediment-bound As(V), and have also shown that a stable enrichment culture of Fe(III)-reducing bacteria was able to mobilize arsenic (as As(III)) from sediments collected from West Bengal (Islam et al., 2004). To identify the Fe(III)-reducing bacteria that may play a role in the reduction of As(V) and mobilization of As(III), we made a detailed molecular analysis of this enrichment culture. It was dominated by a close relative of Geothrix fermentans, but the type strain of this organism was unable to conserve energy for growth via the dissimilatory reduction of As(V), or reduce As(V) present in a defined medium containing fumarate as the electron acceptor. Furthermore, when the cells were grown using soluble Fe(III)-citrate as an electron acceptor in the presence of As(V), bacterial Fe(III) reduction resulted in the precipitation of the Fe(II)-bearing mineral vivianite in 2 weeks. This was accompanied by the efficient removal of As from solution. These results demonstrate that Geothrix fermentans, in common with other key Fe(III)-reducing bacteria such as Geobacter sulfurreducens, does not reduce As(V) enzymatically, but can capture arsenic in Fe(II) minerals formed during respiration using Fe(III) as an electron acceptor. Thus, the reduction of arsenic-bearing Fe(III) oxide minerals is not sufficient to mobilize arsenic, but may result in the formation of Fe(II) biominerals that could potentially act as sinks for arsenic in sediments. Additional mechanisms, including dissimilatory As(V) reduction by other specialist anaerobic bacteria, are implicated in the mobilization of arsenic from sediments.
The study aim was to establish and quantify suicide risk following acute admissions for all major physical illnesses, for confirmatory purposes, from two independent information sources from different countries.
Methods
Record linkage of inpatient and death certificate data for 11 004 389 acute admissions for physical illnesses in England and 713 496 in Wales. The main outcome measure was standardised mortality ratios (SMRs) for suicide at 1 year following discharge from hospital.
Results
There were 1781 suicides within 1 year of discharge in England (SMR = 1.7; 95% = 1.6–1.8) and 131 in Wales (SMR = 2.0; 1.7–2.3). Of 48 major physical illnesses that were associated with at least eight suicides in either country, there was high consistent suicide mortality (significant SMR >3) in both countries for constipation (SMR = 4.1 in England, 7.5 in Wales), gastritis (4.4 and 4.9) and upper gastrointestinal bleeding (3.4 and 4.5). There was high suicide mortality in one country for alcoholic liver disease, other liver disease and chronic pancreatitis; for epilepsy and Parkinson's disease; for diabetes, hypoglycaemia and hypo-osmolality & hyponatraemia; and for pneumonia, back pain and urinary tract infections.
Conclusions
There is little or no increased suicide mortality following acute admissions for most physical illnesses. Much of the increased suicide mortality relates to gastrointestinal disorders that are often alcohol related or specific chronic conditions, which may be linked to side effects from certain therapeutic medications. Acute hospital admissions for physical illnesses may therefore provide an opportunity for targeted suicide prevention among people with certain conditions, particularly alcohol related disorders.
Absorption and translocation of 14C-dicamba (3,6-dichloro-o-anisic acid) in seven salt formulations were determined 60 h after application to leaves of soybean [Glycine max (L.) Merr. ‘Williams’] grown in nutrient solution. The dimethylamine (DMA) formulation was consistently absorbed and retained in the plant in amounts equal to or greater (46% of recovered 14C) than other formulations (which averaged 19% of recovered 14C) when applied without surfactant. Absorption and subsequent retention of the DMA formulation in the plant was least affected of all formulations by the addition of a surfactant. With a surfactant, absorption of the DMA, monoethanolamine (MEA), and inorganic salt formulations was similar (>75% of recovered 14C). Addition of seven surfactants to the K-salt of dicamba increased both the amount of 14C absorbed by 35 to 56% and the amount recovered in the plant. All surfactants except one enhanced absorption of the K-salt of dicamba to a similar degree. Dicamba exhibited predominantly symplastic translocation with the majority of 14C being recovered in the new second trifoliolate leaves and nutrient solution. As much as 66% of the radioactivity absorbed through the leaves was exuded by roots into the nutrient solution 60 h after leaf treatment.
The last interglacial, commonly understood as an interval with climate as warm or warmer than today, is represented by marine isotope stage (MIS) 5e, which is a proxy record of low global ice volume and high sea level. It is arbitrarily dated to begin at approximately 130,000 yr B.P. and end at 116,000 yr B.P. with the onset of the early glacial unit MIS 5d. The age of the stage is determined by correlation to uranium–thorium dates of raised coral reefs. The most detailed proxy record of interglacial climate is found in the Vostok ice core where the temperature reached current levels 132,000 yr ago and continued rising for another two millennia. Approximately 127,000 yr ago the Eemian mixed forests were established in Europe. They developed through a characteristic succession of tree species, probably surviving well into the early glacial stage in southern parts of Europe. After ca. 115,000 yr ago, open vegetation replaced forests in northwestern Europe and the proportion of conifers increased significantly farther south. Air temperature at Vostok dropped sharply. Pulses of cold water affected the northern North Atlantic already in late MIS 5e, but the central North Atlantic remained warm throughout most of MIS 5d. Model results show that the sea surface in the eastern tropical Pacific warmed when the ice grew and sea level dropped. The essentially interglacial conditions in southwestern Europe remained unaffected by ice buildup until late MIS 5d when the forests disappeared abruptly and cold water invaded the central North Atlantic ca. 107,000 yr ago.
We used laboratory and field feeding trials to investigate adult carabidbeetle preferences for three brassicaceous weed species (rapeseed, wildmustard, and field pennycress) that are pests in canola. All carabid speciespreferred seeds of rapeseed most and those of field pennycress least andshowed intermediate preference for wild mustard seeds. Beetles highlypreferred imbibed seeds of all three weed species. Activity–density ofcarabids and mean weed seed removal were highly correlated in field plots ofcanola, with activity–density accounting for 67% of the observed variationin seed removal. Our study indicates that seed consumption among carabids isinfluenced by several factors, including weed species, physiological stateof seeds, and carabid activity–density. Carabid seed predation issignificant in canola agroecosystems; therefore, understanding theseinfluences has implications for ecological weed management.