A series of new laboratory experiments explore the transient flow in an enclosed space of depth
$H$, which is subject to an upward displacement ventilation flux,
$Q_V$, and which contains a localised heat source of buoyancy flux
$F_s$, when the buoyancy of the ventilation air changes by
$\Delta g'$. Initially, the plume, produced by the heat source, entrains the ventilation air, leading to a two-layer stratification which depends on the dimensionless strength of convection,
$\mu \propto F_s^{1/3}H^{5/3}/Q_V$. When the buoyancy of the ventilation air decreases,
$\Delta g' \lt 0$, a new layer of relatively dense fluid grows next to the floor. The fluid entrained by the plume from this new layer causes the plume to intrude between the original upper and lower layers. For a sufficiently large decrease in buoyancy,
$|\Delta g' Q_V /F_s| \gt 1$, then as the new lower layer grows, the plume eventually becomes negatively buoyant relative to the original lower layer and intrudes between the new lowest layer and the original lower layer. When the buoyancy of the air supply increases,
$\Delta g'\gt 0$, it mixes with the fluid in the original lower layer. If the increase in buoyancy is sufficient,
$\Delta g' Q_V/F_s\gt 1$, then the new supply air eventually also mixes with the original upper layer. In each case, a new two-layer stratification becomes re-established. We propose new models for the evolution of the transient flow, assuming that the buoyancy profile can be approximated by a staircase of well-mixed layers. These layers are emptied or filled through the action of the plume and ventilation. We find that the model predictions are consistent with our new experiments in each of the four regimes. We conclude by discussing the implications of these transient flows for thermal comfort and the mixing of contaminants into the occupied lower region of the space.