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This abstract was awarded the student prize for best poster presentation.
Diet represents one of the most influential modifiable factors shaping gut microbial composition and metabolism. Prebiotics, polyphenols, and proteins each affect the microbiome through distinct mechanisms. Prebiotics, such as the dietary fiber Inulin, promote growth of saccharolytic bacteria and SCFA production(1). Bioactive plant polyphenols, such as grapeseed extract, can modulate microbial metabolism, exert antimicrobial properties against select pathogens, and generate bioactive metabolites involved in host inflammatory processes(1). Whey protein serves as a substrate for proteolytic bacteria and microbial amino acid bio-transformations, affecting microbial composition and metabolite profiles(2). While these components have established individual effects, their combined influence remains underexplored. This study examined the individual and combined effects of pre-digested inulin, grapeseed extract, and whey protein isolate on gut microbial composition and metabolism using the MiGut in vitro colon model.
The novel MiGut platform is a miniaturised triple-stage continuous flow in vitro gut model(3) that simulates proximal to distal human colon environments. We used MiGut to undertake a 6-week experiment using eight MiGut reactors (one per treatment condition for each of two healthy human donors). Donor faecal inocula were introduced, followed by a 2-week equilibration period establishing steady-state conditions. Experimental treatments (inulin, grapeseed extract, whey protein isolate, or a combination of all three) were added daily at doses equivalent to dietary recommendations for 2 weeks. All treatments underwent pre-digestion using the INFOGEST in vitro digestion protocol prior to addition(4). This was followed by a 2-week recovery phase without supplementation to assess microbiome elasticity. Samples were collected at each phase from all colon regions.Statistical analysis used a linear mixed-effects model, with Treatment, State, and Vessel as fixed effects, and Donor as a random effect. Significance was assessed using Type III ANOVA with p < 0.05.
PCR analysis revealed distinctive treatment effects relative to baseline. All fold change values reflect differences in microbial abundance compared to post-equilibration levels. Significant treatment effects were observed for Akkermansia, Bacteroides, Bifidobacterium, and Enterococcus (all p<0.05). Inulin increased Bifidobacterium 1.9-fold while reducing Enterobacteriaceae. Whey protein increased Lactobacillus 3.8-fold; grapeseed extract increased Bacteroides by 9.3-fold (p<0.05). The combination treatment demonstrated distinct effects from individual components, enhancing Bifidobacterium populations (2.4-fold increase, p<0.05), but unexpectedly reduced beneficial butyrate-producing bacteria like Roseburia, unlike the individual treatments. Temporal and spatial variations were detected for multiple bacterial targets. Upcoming 16S rRNA sequencing and SCFA analysis will provide deeper taxonomic and functional context.
Findings confirm the importance of assessing individual dietary components and their interactions in microbiome research. The MiGut platform was effective in modelling microbial responses to pre-digested nutrients. Microbiome profiling and metabolomics will provide deeper insights into functional outcomes. This work advances understanding of how multi-nutrient approaches may optimise microbiome-targeted interventions for gut health.
Chapter 3 synthesizes analyses of changes in both social justice movements and legal and policy institutions to broaden our understanding of interconnections among segregation, environmental disparities, and structural vulnerabilities in low-income communities of color. The Buchanan case highlights a relatively narrow framing of land-use injustice in the early twentieth century: zoning as a tool of racial segregation in housing. Throughout the twentieth century, the struggle for land use justice broadened to address the deep structural inequalities and systemic marginalization of all low-income communities of color, including land-use policies creating disparities in environmental conditions, community infrastructure, and vulnerabilities to disasters, shocks, and change. As both grassroots movements and institutions have evolved to grapple with the persistence and complexity of land use injustice in the United States, building the capacities, power, and resilience of low-income communities of color is critical to transformation and justice, and this growing focus on community capacities has come to characterize land-use justice movements.
Transcultural ethnography is about understanding the flows of culture and its consequences as organizations expand their global reach across national boundaries. This scope of research focus beyond a company’s nation of origin is precisely what distinguishes international business from other types of business studies that are primarily domestic. Yet, the concept of national borders is fraught with controversy both in real world actuality as well as in ivory tower discussions. An ethnographic perspective that considers both culture and borders as concepts that are often overdetermined in our efforts to put together aggregated understandings of a world increasingly characterized by cultural flows as well as barriers is essential to theory building. Since transcultural ethnography involves research at globally distributed sites, a comprehensive understanding of how changing cultural contexts affect multinational enterprises (MNEs) and in turn the reciprocal effects of the MNEs on the host environments is essential to advancing transnational theory.
Chapter 1, “‘We Are Not Immune’: A New Branch of the Feminist Women’s Health Movement,” begins by describing the emergence of a new coalition of feminists who turned their attention to the HIV epidemic in an attempt to understand how the virus would impact women. Together they realized that HIV was killing women more often than the those in charge of the AIDS response acknowledged. The failure to recognize and respond to issues facing women with HIV was due, in part, to the Centers for Disease Control and Prevention definition of AIDS that did not include gynecological infections. The incomplete definition of AIDS resulted in a lack of data on women with HIV and impacted the Social Security Administration’s determinations of who should receive benefits. Allying with lawyers and fellow activists, feminists set out to challenge the law and science of the epidemic.
Traditional pastoral practices have maintained Alpine grasslands over thousands of years, and Alpine biodiversity now depends on these practices. Grasslands are also central to the identity of pastoral communities: They are biocultural landscapes. Across the Alps, these landscapes are now threatened by high rates of agricultural land abandonment as traditional, labor-intensive agricultural methods become uneconomic, and small-scale development increases. The Autonomous Province of Bozen/Bolzano-South Tyrol, Italy, experiences some of the lowest rates of land abandonment and high rates of grassland retention. The case study explores the functions of regulatory intervention and coordination, two of the regulatory functions advanced by this book’s CIRCle Framework of regulatory functions for addressing cumulative environmental problems. It investigates how a diverse set of regulatory interventions provides for maintaining and restoring grasslands in South Tyrol, and how diverse forms of coordination – links between areas of laws, coordinating institutions, and dispute resolution processes – facilitate implementation in a context of deep multilevel governance.
In the course of seeking indigenous documentation in a European-centered colonial archival repository, we uncovered a collection of African sources that highlight the literary work of African and Asian literate agents. The research enabled us to identify numerous indigenous African and Asian writings within an archive originally intended to support the Portuguese colonial administration. This article presents an archival survey on African documentation from Mozambique held in the Overseas Historical Archive (Arquivo Histórico Ultramarino), in Lisbon, Portugal.
Edited by
Camran R. Nezhat, Stanford University School of Medicine, California,Farr R. Nezhat, Nezhat Surgery for Gynecology/Oncology, New York,Ceana Nezhat, Nezhat Medical Center, Atlanta,Nisha Lakhi, Richmond University Medical Center, New York,Azadeh Nezhat, Nezhat Institute and Center for Special Minimally Invasive and Robotic Surgery, California
Chapter 1 sets out the methodology of the work-task approach in more detail before providing an overview of the findings. It introduces the sources used, the challenges they present, and the methods adopted to mitigate those challenges, as well as presenting the overall results of our research.
Chapter 5 examines the myriad ways Africans contested their indentureship, arguing that these cultural and economic choices by first-generation recaptive Africans shaped the formation of African work on Grenada. Like the actions taken by enslaved Africans, recaptured Africans left estates temporarily or permanently to establish and maintain bonds with shipmates or those of similar 'nations'. For the majority, African languages were spoken along with French, and church attendance was irregular. Moreover, a preference was expressed for Roman Catholicism because it was compatible with their religious cultures. While many of these choices indicate adaptation to a creolised society, they also demonstrate that adaptation was gradual and measured.
This chapter is the first of three examinations of dominant copyright reversion traditions (UK, US, EU) throughout this book. It traces how reversion rights were present in the very first copyright statute, the 1710 Statute of Anne. It demonstrates how different iterations of reversion rights were hamstrung by poor design and undermining by rightsholders (e.g. by contracting around the intended effects of these provisions). It then canvasses modern developments in reversion rights across the Commonwealth (like in Canada and South Africa).
Chapter 2, “Litigating Risk: The Law and Politics of Disease in the Administrative State,” turns to the litigation and activism that resulted in the shift in the Centers for Disease Control and Prevention definition of AIDS and turned attention to women’s risk of contracting HIV. The chapter tracks how litigation and advocacy were central to the shift in the CDC definition of AIDS. Feminist success would result in many more women being diagnosed with HIV, resulting in a greater ability to access benefits. This life-changing shift would mark a major victory for the feminist women’s health movement.
Samuel Goyvaerts explores the notion of liturgical pastoral, the roots of which are to be found in the Liturgical Movement. It is an umbrella term that makes one understand how liturgy is intrinsically connected to the Church’s diakonia, kerygma, and koinonia. Liturgy is essential for building up the Church, for faith formation, and for the faithful’s service to the world.
Despite the scope and demands of his critical philosophy, Kant provided no systematic account of language. In Kant’s works the discussion of language is nowhere close to the attention he gives to other major fields of philosophical inquiry such as cognition, science, human agency, or art. This apparent neglect is mirrored in the literature on Kant and Kantian themes. Contemporary debates on Kant’s ethics, epistemology, and aesthetics typically ignore linguistic presuppositions and implications.