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Using pore-resolved direct numerical simulation (DNS), we investigate passive scalar transport at a unit Schmidt number in a turbulent flow over a randomly packed bed of spheres. The scalar is introduced at the domain’s free-slip top boundary and absorbed by the bed, which maintains a constant and uniform scalar value on the sphere surfaces. Eight cases are analysed, which are characterised by friction Reynolds numbers of ${\textit{Re}}_\tau \in [150, 500]$ and permeability Reynolds numbers of ${\textit{Re}}_{{\kern-1pt}K} \in [0.4, 2.8]$, while flow depth-to-sphere-diameter ratios vary within $h/D \in \{ 3, 5, 10 \}$ and the roughness Reynolds numbers lie within $k_s^+ \in [20,200]$. For cases with comparable ${\textit{Re}}_\tau$, the permeable wall behaviour enhances scalar absorption, as indicated by increases in the Sherwood number and the scalar roughness function $\Delta c^+$ over ${\textit{Re}}_{{\kern-1pt}K}$. At progressively higher ${\textit{Re}}_{{\kern-1pt}K}$, the scalar absorption diverges increasingly from the momentum absorption, as its distribution peaks deeper below the crests of the sphere pack and spreads over a wider vertical region. The fixed-value scalar boundary condition emphasises certain similarities between the scalar and velocity fields. Near-interface scalar fluctuations are correlated with streamwise velocity fluctuations, and the turbulent Schmidt number remains close to its value in the free-flow region. Compared with zero-flux scalar boundary conditions, prescribing a uniform scalar value on the sphere surfaces reduces spatial heterogeneity within the pore space, thereby limiting both dispersive transport and the form-induced production of temporal scalar fluctuations.
We join the theories that describe the orientation, treated as a tensor, of liquid crystals and the agitation of inelastic grains to obtain a mathematical model of non-spherical particles contained in a quasi-2D square box and driven into dissipative collisions through the vibration of two of the four flat walls, in the absence of gravity and mean flow. The particle agitation induces spatial inhomogeneities in the density and the isotropic–nematic transition to take place somewhere inside the box, if the particle shape is sufficiently far from spherical. We show quantitative agreement between the theory and discrete numerical simulations of ellipsoids of different length-to-diameter ratio. We need to fit two dimensionless parameters that were not previously available or determined in different configurations. These parameters, of order unity and weakly dependent on the shape of the particles, are indicative of the resistance to alignment distortion associated with entropic elasticity.
Recently, data-driven methods have shown great promise for discovering governing equations from simulation or experimental data. However, most existing approaches are limited to scalar equations, with few capable of identifying tensor relationships. In this work, we propose a general data-driven framework for identifying tensor equations, referred to as symbolic identification of tensor equations (SITE). The core idea of SITE – representing tensor equations using a host–plasmid structure – is inspired by the multidimensional gene expression programming approach. To improve the robustness of the evolutionary process, SITE adopts a genetic information retention strategy. Moreover, SITE introduces two key innovations beyond conventional evolutionary algorithms. First, it incorporates a dimensional homogeneity check to restrict the search space and eliminate physically invalid expressions. Second, it replaces traditional linear scaling with a tensor linear regression technique, greatly enhancing the efficiency of numerical coefficient optimization. We validate SITE using two benchmark scenarios, where it accurately recovers target equations from synthetic data, showing robustness to noise and flexible expressive capability. Furthermore, SITE is applied to identify constitutive relations directly from molecular simulation data, which are generated without reliance on macroscopic constitutive models. It adapts to both compressible and incompressible flow conditions and successfully identifies the corresponding macroscopic forms, highlighting its potential for data-driven discovery of tensor equation.
One of the challenges with modelling subsurface flows is the uncertainty in measurements of geological properties, mostly due to limited resolution in observation methods. Many subsurface flows can be modelled as a gravity current, which, for uniform material properties and power-law injection rate, has a well-characterised similarity solution. The similarity solution forms a dynamical attractor that is typically approached rapidly from a host of initial conditions. Here, we consider the impact of transverse variations to the permeability field by performing a perturbation analysis of the self-similar spreading. This treats the response as perturbations to the self-similar flow. We restrict our focus to permeability fields that vary laterally, or across the flow, starting with the simple case of a sinusoidal perturbation to a uniform permeability. At early times, the height and nose position of the current are determined by the local permeability, and deviations to the height and nose grow at the same rate as the mean, and proportional to the amplitude, of the permeability variation. The transition between the early and late time regimes is governed by the wavelength of the permeability. At late times, lateral spreading between high and low permeability streaks is dominant; the height deviations decay, and the nose deviations approach a steady state. The magnitudes of both depend on the product of the wavelength and amplitude of the permeability. The single mode sets the groundwork for examining more complex, multimodal permeabilities, which are more representative of real geological structures.
Fully resolving turbulent flows remains challenging due to a turbulent systems’ multiscale complexity. Existing data-driven approaches typically demand expensive retraining for each flow scenario and struggle to generalize beyond their training conditions. Leveraging the universality of small-scale turbulent motions (Kolmogorov’s K41 theory), we propose a scale-oriented zonal generative adversarial network (SoZoGAN) framework for high-fidelity, zero-shot turbulence generation across diverse domains. Unlike conventional methods, SoZoGAN is trained exclusively on a single dataset of moderate-Reynolds-number homogeneous isotropic turbulence (HIT). The framework employs a zonal decomposition strategy, partitioning turbulent snapshots into subdomains based on scale-sensitive physical quantities. Within each subdomain, turbulence is synthesized using scale-indexed models pretrained solely on the HIT database. A SoZoGAN demonstrates high accuracy, cross-domain generalizability and robustness in zero-shot super-resolution of unsteady flows, as validated on untrained HIT, turbulent boundary layer and channel flow. Its strong generalization, demonstrated for homogeneous and inhomogeneous turbulence cases, suggests potential applicability to a wider range of industrial and natural turbulent flows. The scale-oriented zonal framework is architecture-agnostic, readily extending beyond generative adversarial networks to other deep learning models.
This study describes the reproductive systems of male and female Johngarthia lagostoma, a land crab endemic to South Atlantic oceanic islands, focusing on spermatozoa production and storage. Specimens from Trindade Island (Brazil) were analysed for anatomy, histology, and histochemistry. The male system includes a pair of tubular testes showing different stages of spermatogenesis and spermiogenesis, leading to mature spermatozoa. These move to the anterior vas deferens (AVD), which has proximal and distal portions, the latter containing coenospermic spermatophores surrounded by secretion type I, reactive to proteins and acidic and neutral polysaccharides. The median (MVD) and posterior (PVD) vas deferens produce type II (strongly protein-reactive) and type III (weakly protein-reactive) secretions. Accessory glands between the MVD and PVD produce the same secretions plus a secretion type IV, reactive to neutral polysaccharides. These mix with the spermatophores and other vas deferens secretions, increasing the PVD’s secretion volume, crucial for the initial release of spermatophores into the seminal receptacle. The female reproductive system features voluminous seminal receptacles connected to the ovary, comprising mesodermal and ectodermal regions classified as ventral-type connection. Plugs and sperm packets are absent, with the seminal receptacles filled with free spermatozoa, suggesting dehiscence occurs shortly after sperm transfer. The influence of male and female secretions on this process is suggested, alongside their roles in sperm maintenance and fertilization facilitation.
We study the force exerted by the uniform flow of a Bingham fluid around two- and three-dimensional particles in the regime of slow creeping flow and relatively weak yield stress. Matched asymptotic expansions are employed to couple a viscously dominated Stokes flow close to the particle with a far field in which the yield stress and viscous stresses are comparable. The far-field region is therefore modelled as a Bingham fluid driven by a point force at the origin (i.e. a viscoplastic Stokeslet). It features the full nonlinearity of the viscoplastic rheology, and its solution is computed through direct numerical simulation. Asymptotic matching then leads to a quasi-analytical expression for the drag force in terms of the dimensionless Bingham number ${\textit{Bi}}$, which measures the magnitude of the yield stress relatively to viscous effects at the particle scale. We deploy this methodology to determine the drag force on a sphere in three dimensions, and circular and elliptic cylinders in two dimensions, confirming our asymptotic predictions by comparison with full numerical simulations of the motion. We also generalise the three-dimensional result to arbitrary particles. The viscoplastic correction to the Newtonian drag in three dimensions scales as ${\textit{Bi}}^{1/2}$. In two dimensions, however, the effects of viscoplasticity are non-negligible at leading order. The drag varies with $[\ln (1/{\textit{Bi}})]^{-1}$, but this asymptotic result is only approached very slowly. Instead, an accurate representation of the drag is derived in terms of a single algebraic relation between the drag and the Bingham number.
Myostatin (MSTN), a member of the transforming growth factor-β superfamily, negatively regulates skeletal muscle growth in vertebrates. In teleosts, gene duplication has produced mstn1 and mstn2 paralogues, which often differ in structure and expression. This study compares mstn1 and mstn2 in two high-value mariculture-relevant carangids of the Indo-Pacific region, Trachinotus mookalee and Trachinotus blochii. We report, for the first time, the complete gene structures of mstn1 and mstn2 in T. mookalee (3777 bp from Tm-mstn1 and 2075 bp from Tm-mstn2) and describe their counterparts in T. blochii (3836 bp from Tb-mstn1 and 2147 bp from Tb-mstn2). Notably, mstn1 and mstn2 shared only ∼53% sequence identity within the same species. Interestingly, we noted a CA-repeat tandem sequence in intron 2 (35 bp in Tm-mstn1 and 47 bp in Tb-mstn1), providing a potential microsatellite marker. Promoter analysis suggested more complex transcriptional regulation in T. blochii, with a greater number of transcription factor binding sites (47 vs. 43) and E-box motifs (4 vs. 2). Predicted miRNA binding site revealed both shared (14) and species-specific sites (two sites in Tm-mstn1, and one in Tb-mstn1), indicating differential post-transcriptional regulation. These molecular differences were verified through differential mstn1 expression, with higher mstn1 expression in T. blochii muscle, which might be the reason for the enhanced muscle growth in T. mookalee. The mstn2 expression patterns supported its role in neuroendocrine and reproductive regulation. Overall, this study provides new molecular insights into species-specific growth differences and highlights the functional divergence of mstn genes in marine carangids.
We present an acoustic characterisation of a model-scale wind turbine using large eddy simulation and the acoustic analogy. The analysis is representative of medium-sized turbines with low tip Mach number (${\sim} 0.10$). The fluid dynamic analysis revealed: a turbulent boundary layer over the blades, together with a trailing edge vortex sheet; a complex near-wake structure, including tip and root vortices; an intermediate wake with vortex instabilities triggering leap-frogging and vortex grouping mechanisms; and a far wake characterised by fully developed turbulence. Two primary noise generation mechanisms were identified. The unsteady pressure field over the turbine surface generates tonal noise at the blade passing frequency and a high-frequency broadband noise, associated with the trailing edge vortex sheet (linear-noise contribution). The turbulent wake generates broadband low-frequency noise, driven by the complex fluid-dynamic processes outlined previously (nonlinear noise contribution). The linear part of the noise was found to dominate over the nonlinear one in the acoustic far field, while the opposite is true in the acoustic near field. As a composition of the two contributions to the noise, the directivity exhibits a non-symmetric dipole shape oriented along the flow direction, with lobes recovering symmetry moving from the near to the far field. Finally, analysis of the acoustic decay rates reveals that the linear term in the near field decays according to an $r^{-(n+1)}$ law within the rotor plane, where n is the number of blades, consistent with recent findings on the acoustics of rotating sources.
We investigate the scale-by-scale transfers of energy, enstrophy and helicity in homogeneous and isotropic polymeric turbulence using direct numerical simulations. The study relies on the exact scale-by-scale budget equations, derived from the governing model equations, that fully capture the back-reaction of polymers on the fluid dynamics. Polymers act as dynamic sinks and sources and open alternative routes for interscale transfer whose significance is modulated by their elasticity, quantified through the Deborah number (${\textit{De}}$). Polymers primarily deplete the nonlinear energy cascade at small scales, by attenuating intense forward and inverse transfer events. At sufficiently high ${\textit{De}}$, a polymer-driven flux emerges and dominates at small scales, transferring on average energy from larger to smaller scales, while allowing for localised backscatter. For enstrophy, polymers inhibit the stretching of vorticity, with fluid–polymer interactions becoming the primary enstrophy source at high ${\textit{De}}$. Accordingly, an analysis of the small-scale flow topology reveals that polymers promote two-dimensional straining states and enhance the occurrence of shear and planar extensional flows, while suppressing extreme rotation events. Helicity, injected at large scales, exhibits a transfer mechanism analogous to energy, being dominated by nonlinear dynamics at large scales and by polymer-induced fluxes at small scales. Polymers enhance the breakdown of small-scale mirror symmetry, as indicated by a monotonic increase in relative helicity with ${\textit{De}}$ across all scales.
Contact between fluctuating, fluid-lubricated soft surfaces is prevalent in engineering and biological systems, a process starting with adhesive contact, which can give rise to complex coarsening dynamics. One representation of such a system, which is relevant to biological membrane adhesion, is a fluctuating elastic interface covered by adhesive molecules that bind and unbind to a solid substrate across a narrow gap filled with a viscous fluid. This flow is described by the stochastic elastohydrodynamic thin film equation, which incorporates thermal fluctuations into the description of viscous nanometric thin-film flow coupled to elastic membrane deformation. The average time it takes the fluctuating elastic membrane to adhere is predicted by the rare event theory, increasing exponentially with the square of the initial gap height. When the forces arising from spring-like adhesive molecules are included in the simulations, thermal fluctuations initiate phase separation of domains of bound and unbound molecules. The coarsening process of these unbound pockets displays close similarities to classical Ostwald ripening; however, the inclusion of hydrodynamics affects power-law growth. In particular, we identify a new bending-dominated coarsening regime, which is slower than the well-known tension-dominated case.
As the use of carbon dioxide (CO2)-enhanced oil recovery continues, understanding its impacts on the in situ oil well microbial communities is important. In this study, we update the already substantial investigation into five oil wells of the Olla and Nebo Hemphill fields in the southeastern United States with metagenomics. The Olla field has undergone CO2 injection, whereas the Nebo Hemphill field has not. Under anoxic conditions in Nebo Hemphill, our data suggest that methanogenic archaea are in competition with fermentative bacteria for shared substrates, leading to reduced biogenic methane production. However, in the Olla wells that had introduced CO2, we find genomic evidence for the growth and replication of bacteria able to respire oxygen. Based on the co-occurrence and potential replication of methanogens in these wells, we hypothesise that there are still anoxic niches for methanogens. Oxygen-utilising microbes may utilise phenolic substrates, creating a situation where more material is available for anaerobic growth, counterintuitively increasing the capacity for methane production in these wells. Our data suggest that this consortium may be the reason that greater methanogenesis is seen in these CO2-affected wells, and we hypothesise that the gas injection may have had contaminating air. Therefore, using existing wells for processes such as enhanced oil recovery or carbon capture and storage will not only depend on well history and in situ conditions, but also on the development of in situ microbial networks within modified wells.
The population status of Adélie penguins (Pygoscelis adeliae) on the Antarctic Peninsula highlights opportunities for the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR) to address uncertainty more directly and effectively in its conservation efforts. The heightened uncertainty posed by climate change is testing CCAMLR’s commitment to balancing science and international cooperation in decision-making. Uncertainty underpins some of the justification to postpone reaching a consensus on the establishment of Marine Protected Areas, leaving Adélie penguins vulnerable to change. Two key opportunities to adapt current management approaches emerge: 1) reduce uncertainty by systematically identifying knowledge gaps within CCAMLR’s processes; and 2) integrate uncertainty more explicitly into decisions through a standardized approach to assessing and communicating it.
McEuen Cave (AZ W:13:6 (ASM)) is a large bedrock rockshelter located within an andesitic rocky ash flow tuff/ignimbrite within the Bureau of Land Management’s Fishhooks Wilderness Area near Fort Thomas, Arizona. Exceptional preservation at the site has produced an extensive assemblage of perishable artifacts, including a tremendous quantity of cultigen remains radiocarbon dated between 3600 BP and 1250 BP. In this paper, we provide the results of a new radiocarbon dating effort aimed at identifying additional early Silverbell Interval cultigens and clarifying the intensity and persistence of Early Agricultural Period occupation. Our goal is to better understand the age and extent of early cultivation activities within this high-elevation wilderness and contextualize the remains from this site with the more thoroughly understood co-eval Early Agricultural Period villages evidenced along major riverways such as the San Pedro and Santa Cruz in southern Arizona. [Spanish language abstract: La cueva McEuen (AZ W:13:6 (ASM)) es un gran refugio rocoso de lecho rocoso ubicado dentro de una toba/ignimbrita de flujo de cenizas rocosas andesíticas dentro del área silvestre Fishhooks de la Oficina de Administración de Tierras cerca de Fort Thomas, Arizona. La preservación excepcional en el sitio ha producido un extenso conjunto de artefactos perecederos, incluyendo una tremenda cantidad de restos de cultígenos fechados por radiocarbono entre 3600 BP y 1250 BP. En este documento, proporcionamos los resultados de un nuevo esfuerzo de datación por radiocarbono destinado a identificar cultígenos adicionales del Arcaico tardío-medio y aclarar la intensidad y persistencia de la ocupación del Período Agrícola Temprano. Nuestro objetivo es comprender mejor la edad y el alcance de las actividades de cultivo tempranas dentro de este desierto de gran altitud y contextualizar los restos de este sitio con las aldeas coeval del Período Agrícola Temprano mejor entendidas evidenciadas a lo largo de las principales vías fluviales como el San Pedro y el Santa Cruz en el sur de Arizona.]
An experimental study is performed to control flow separation from a two-dimensional curved ramp using a spanwise pulsed blowing slit jet placed near the separation point of the baseline flow. The momentum-thickness-based Reynolds number $ \textit{Re}_{\theta}$ is 5700. Four control parameters are investigated, including the velocity ratio $\overline{U_{J,c}^{*}}$, duty cycle dc, dimensionless excitation frequency $f_{e}^{{*}}$ and jet blowing angle $\alpha$. The control mechanisms are found to differ from small to large jet angle. Empirical scaling analysis for $\alpha \leq 55^{\circ}$ unveils that $\Delta \overline{C_{p,e}}=f_{1}(\overline{U_{J,c}^{*}}, { d}c, f_{e}^{*}, \alpha , Re_{\theta })$ may be reduced to $\Delta \overline{C_{p,e}}/\varPi (\tau )=f_{2}(\xi )$, where $f_{1}$ and $f_{2}$ are different functions, $\Delta \overline{C_{p,e}}$ is the variation in the pressure coefficient at the end of the ramp under control, $\varPi (\tau )$ is a function of dimensionless duration $\tau$ at which the jet is closed within one excitation period, $\Delta \overline{C_{p,e}}/\varPi (\tau )$ represents the control efficiency, and $\xi$ is a scaling factor that is physically the energy ratio per unit area of the blowing jet to the mainstream. This scaling law is also found to be valid for steady jet control. Several interesting inferences can be made from this scaling law, which provides important insight into the physics of flow separation control.
Analytical expressions for the mean wall-normal velocity and wall shear stress in compressible boundary layers are derived by integrating the mean continuity and momentum equations. In the constant-density limit, the momentum integral formulation recovers the classical Kármán–Pohlhausen equation for incompressible boundary-layer flows. In compressible regimes, particularly under strong pressure gradients, streamwise density gradients are shown to play a crucial role in shaping boundary-layer dynamics. The derived analytical equations are validated against high-fidelity direct numerical simulation data, demonstrating both accuracy and robustness. Furthermore, the analytical equations offer insights into the physical mechanisms of compressible boundary layers, particularly the influence of density gradients. The effect of compressibility on the wall-normal velocity is explicitly demonstrated, highlighting the distinct behaviour of compressible boundary layers compared with incompressible flows. Finally, an analytical expression for the skin-friction coefficient is developed, revealing its close connection to the mean wall-normal velocity at the boundary-layer edge.
The Neolithic of the northeastern Iranian Plateau is defined basically by the materials recovered from the twin mounds of Sang-e Chakhmaq, the West Mound and the East Mound. The radiocarbon dates from these mounds span almost two thousand years, from around 7000 BCE to the last centuries of the sixth millennium BCE, with a chronological hiatus between ca. 6700–6200 BCE. Recent excavations at a proto-ceramic Neolithic site, Rouyan, in the vicinity of Sang-e Chakhmaq, provided occupational evidence, augmented by a series of Radiocarbon dates, which fill in the long-standing temporal hiatus of the Neolithic of the region. Both 14C dates and archaeological evidence from this excavation suggests that Rouyan was founded simultaneously with the West Mound of Sang-e Chakhmaq, but its occupation continued without discontinuity into the fifth millennium BCE. The excavation also yielded a small ceramic assemblage from the earliest deposits of the site, indicating the site’s first settlers were familiar with this technology as early as ca. 7000 BCE.
In this article, we present the first results from radiocarbon dating of the Kirakle-Tobe settlement located in the central part of the Volga River Delta, southern Russia. Archaeological artifacts and 14C measurements on charcoal indicate three stages of settlement development on the Kirakle-Tobe knoll. The oldest 14C age corresponds to the Late Sarmatian period—early 4th century CE. The abundance of archaeological artifacts associated with the 6th–8th centuries CE indicates a long period of occupation. The youngest 14C age presumably corresponds to the Khazarian period (9th century CE). These results suggest dynamic human activity in the central part of the Volga River Delta during the Great Migration Period. These initial results can be used to verify the impact of fluctuations in the Caspian Sea level on the Volga River Delta during the Great Migration Period.
Not all particulate matter carried by fluid flows has constant buoyancy. In some cases, the buoyancy of a particle can change dynamically based on the local flow. We refer to this phenomenon as ‘active buoyancy.’ Although actively buoyant particles are found throughout nature, their dynamics is not well understood, particularly when they are also highly inertial. Motivated by the problem of the transport of firebrands in wildfires, whose effective buoyancy is modulated by conductive and convective heat transfer to the surrounding fluid, we conducted a series of experiments to investigate the effects of active buoyancy on particle settling in quiescent fluid. We find that, depending on the control parameters, active buoyancy can either hinder or enhance settling, in some cases to a large extent. The details of this settling modulation, however, cannot be simply captured by any single control parameter. Analysis of the trajectories of the falling particles showed that they fall along nearly sinusoidal paths even though the particle Reynolds number is higher than expected for this regime, suggesting that active buoyancy may act to stabilise their wakes. Our results suggest both that models of actively buoyant particles such as firebrands must account for the effects of active buoyancy and that there is still much to be understood about the behaviour of these complex particles.