Objectives/Goals: 1.To develop the next generation of translational scientists in PR. 2. To equip pre-university/undergraduate students with the necessary skills, knowledge, and experiences for their academic and professional development from a holistic perspective of life. 3. To strengthen the technological-translational science (TS) infrastructure. Methods/Study Population: Develop the Center for Research Education and Science Communication-Counseling Opportunities (CRESCO) to provide support to PuS and UgS in TS skills, statistics, scientific writing, emerging technologies, mentoring-tutoring and counseling-orientation; organize/launched the ACADEMIA-TS (ACAD-TS) with the inaugural activity to provide training/student development in TS to PuS and UgS, including peer-mentoring with graduate students (GS); create the Small Project in Translational Science (SPTS) program to offer PuS and UgS hands-on experiences with participation in up-to-date research in TS under the mentorship of a principal investigator-mentor; offer the Title V Annual Symposium in TS; and acquire computers/laboratory supplies/equipment to health sciences undergraduate schools. Results/Anticipated Results: CRESCO established a training infrastructure by acquiring 535 licenses (Consensus, Scite.ai, Mind-Graph) to integrate AI into research and offered 122 mentoring sessions. There were 296 participants in four new workshops. In ACAD-TS’ inaugural activity (~200 attendees), 67 PuS expressed interest in the ACAD-TS; 12 PuS are part of it, and 38 PuS participated in a counseling workshop. The SPTS program integrated course enrolled 10 students (8 from other UPR campuses) and created five teams involving 26 students (8 PuS, 16 UgS, 2 GS) mentored by 5 Principal Investigators (PIs), representing 15 institutions. The 14th Annual Symposium had 136 attendees, with a 100% satisfaction rate (n=44). The technology/TS infrastructure was upgraded with 50 new computers and supplies/minor equipment for health science schools. Discussion/Significance of Impact: Established a comprehensive model to develop early-career translational scientists, including a course, SPTS, and counseling workshops. Integrating AI tools, focused mentorship, and enhanced infrastructure strengthened participants’ integral development, confirming this model boosts research capacity for the future TS workforce.

Objectives/Goals: The plasma membrane (PM) is the nexus for cell signaling, where lipid composition governs receptor organization and T cell function. We aim to define PM dynamics in T cell states and develop PM-based strategies to preserve anti-tumor activity. Methods/Study Population: As such, we induced a dysfunctional T cell state, known as T cell exhaustion, through chronic stimulation or TGF-β1, hypoxia, and tumor cell co-culture and measured membrane biophysical properties through ratiometric confocal imaging of molecular probe Di-4-ANEPPDHQ. Complementary biochemical data from shotgun lipidomic analysis, immunofluorescence staining, and biochemical fluorescent assays paint a more complex picture of membrane fluidity changes during T cell exhaustion. Results/Anticipated Results: In both cases, we observed decreased PM rigidity compared to acute or normoxic T cell activation, specifically in T cells with upregulated PD1, LAG-3, and Tim-3. Dysfunctional T cells also exhibit changes in specific lipid species. This includes increased free cholesterol and decreased esterified cholesterol among other species. Discussion/Significance of Impact: Adoptive cell therapy is limited in solid tumors due to a hypoxic, suppressive TME that drives T cell exhaustion. Our data suggest exhausted T cells have fluid plasma membranes that impair synapse formation. Future work will validate this in patient-derived xenograft/TCR-T models that will pave the way for clinical translation.

Objectives/Goals: To assess the association between pollution and CVD outcomes in a high-risk CAD population, and investigate how social drivers intersect with this association. Methods/Study Population: 1,602 participants enrolled in the Emory Cardiovascular Biobank were stratified into high vs. low pollutant exposure groups based on PM₂.₅ (median 10.6 μg/m³), NOx (median 39.4 ppb), and CO (median 635 ppb) levels. Demographics, cardiovascular (CV) risk factors, comorbidities, and SVI domains including socioeconomic status (RPL_THEME1), household characteristics (RPL_THEME2), racial/ethnic minority status (RPL_THEME3), housing and transportation (RPL_THEME4), and overall SVI ranking (RPL_THEMES) were compared between groups. Cox proportional hazards models were used to assess associations between pollutants and CVD outcomes (CV death, myocardial infarction [MI], stroke, heart failure, and the composite MACE. Results/Anticipated Results: Higher PM₂.₅ exposure was associated with a greater prevalence of hypertension (82.7% vs. 74.6%, p<0.001), female sex (39.7% vs. 34.1%, p=0.023), and elevated SVI scores (RPL_THEME1–4, all p<0.05). Similar gradients were observed across NOx and CO strata, particularly in SVI metrics (p<0.001). In fully adjusted models, MACE were associated with PM₂.₅ (HR 1.73, 95% CI: 1.22–2.44, p = 0.002) and CO (HR 1.00, 95% CI: 1.0005–1.003, p = 0.005) levels. Additionally, HF admissions were associated with PM₂.₅ levels (HR 1.16, 95% CI: 1.05–1.28, p = 0.003). SVI measures interacted significantly with the impact of NOx on HF risk [RPL_THEME1 (HR 1.03, p = 0.033) and RPL_THEME2 (HR 1.05, p = 0.008)] and stroke risk [RPL_THEME4 (HR 1.07, p = 0.025)]. Discussion/Significance of Impact: In patients with CAD, higher PM₂.₅ exposure is independently associated with risk of MACE. Social vulnerability amplified the adverse risk relationships for NOx exposure highlighting the need to consider neighborhood-level risk in pollution-related CV disease prevention.

Objectives/Goals: Integrating lived experiences of people like patients and caregivers across translational research increases responsivity to local need and likelihood of meaningful results. The Community Health Consultant (CHC) Program is a dynamic, formalized source of lived experience advisors for the Institute of Clinical and Translational Sciences (ICTS). Methods/Study Population: Established in 2023, the CHC Program comprises: 1) ongoing recruitment of individuals committed to sharing their lived experiences and perspectives with researchers; 2) creation of profiles which detail an individual’s neighborhood involvement, lived experiences with health and social conditions, and interest in types of advisor activities; 3) matchmaking individuals with relevant lived experiences to community studios, boards, and community grant review processes; 4) a formalized compensation structure which prioritizes efficiently compensating consultants at a rate that demonstrates the value that lived experiences brings to research processes; and 5) consistent feedback loops with the CHC Program as a whole as well as with individual consultants. Results/Anticipated Results: To date, 152 individuals with wide-ranging backgrounds have joined as lived experience advisors to ICTS research. A sample of their collective individualities: * Chris is a community health worker in East Saint Louis. He wanted to be a liaison to the areas he says researchers do not go. “Researchers need to listen to people like me,” he says, “the urban community feels like it’s lost its voice.” * Terry is a mayor in north St. Louis. He says if a researcher is focused on a particular group, they should get “real feedback on how we feel these things benefit us or not benefit us.” * Melvia is an active senior citizen. She says, “The connection to the community is a wealth of information.” In 12 months, activities have included community studios (46), grant review (32), advisory committee linkages (6), and faculty lunch meetings (3). Discussion/Significance of Impact: A dynamic, formalized source of lived experience advisors is critical for mobilizing engagement activities to support ICTS researchers. A community engagement program acts as a natural coordinating hub, building and maintaining individual relationships while matchmaking and providing warm handoffs for lived experience linkages.

Objectives/Goals: Translational research teams span varied roles, disciplines, and institutions. They rely on information to collaborate, communicate, and coordinate. Information should flow seamlessly for collaborative success, yet we know little about the collective information needs and challenges of such teams, representing a translational science problem. Methods/Study Population: We report on the Information Management Prototype for Clinical and Translational Research (IMPACT-CTR) study which explored how clinical and translational research teams (CTRTs) manage their information while conducting collaborative research. Participants across 11 US-based CTRTs (n=52) participated in brief surveys and interviews about their collaborative processes. We are following the Translational Team Science Hierarchy of Needs (Kelly et al., 2023) framework in our thematic analyses, where we are sequentially examining: (1) the foundation of information infrastructure (tools and resources), (2) teams’ information management practices, and (3) how these practices affect psychological safety of team members, which then impact “translational nirvana” or the research synergy of CTRTs. Results/Anticipated Results: Preliminary analysis revealed that information was crucial to CTRTs’ functionality but often overlooked. Team members moved through the research lifecycle by juggling numerous types of information spread across multiple tools, while balancing their personal information styles with those of the collective. Subsequent analyses will investigate the challenges, workarounds, and lessons CTRT members have learned while managing their information and relying on each other as information resources, and the extent to which these factors impact their scientific and operational work, as well as their team culture. We will thus develop and present a conceptual framework of how CTRTs’ information behaviors can facilitate or impede high-impact collaborative research. Discussion/Significance of Impact: We will present reproducible team science practices that can enhance the process of scientific research through evidence-based information management practices that are both effective and feasible for CTRTs. In doing so, we will identify information-related barriers that hinder the efficiency of the translational research continuum.

Objectives/Goals: To evaluate how establishing a food pantry through partnerships with Bread of the Mighty and Feeding Northeast Florida influenced community engagement at UF HealthStreet between 2023 and 2025. Methods/Study Population: UF HealthStreet, a community engagement program, became part of the Healthy Pantry network through partnerships with Bread of the Mighty and Feeding Northeast Florida. 14,678 people are part of the UF HealthStreet registry and 47% self-reported experiencing food insecurity. To evaluate impact, engagement data from two 12-month periods were compared: June 1, 2023–May 31, 2024 (pre-food pantry) and June 1, 2024–May 31, 2025 (food pantry year). Metrics included HNAs, referrals, and visits analyzed to assess engagement changes. Results/Anticipated Results: Community engagement increased from 2,705 visits (pre-pantry) to 7,726 (post-pantry), including 4,243 connected through pantry visits. The number of people visiting UF HealthStreet rose from 1,210 (pre-pantry) to 2,286 (post-pantry), demonstrating broader community reach. Average monthly HNAs rose from 35 to 52 (~1.5x increase). Referrals grew slightly from 2,093 to 2,129, reflecting sustained service linkages amid expanded participation. Becoming a Healthy Pantry site strengthened UF HealthStreet’s capacity to address food insecurity and reduce health disparities. Discussion/Significance of Impact: Food bank partnerships expanded UF HealthStreet’s reach and strengthened community trust. More visits and return participants reflect deeper engagement. Integrating food access into health outreach advances translational science and helps address health disparities.

Objectives/Goals: Meaningful community engagement is critical to trustworthy translational research, yet engagement strategies outside structured meetings are rarely described. For a study of HIV PrEP implementation on college campuses, a Community Involved Board (CIB) participated in between-session activities (BSAs), described below. Methods/Study Population: Six CIB members were recruited to attend bi-monthly sessions from 2024 to 2025. The research team developed each BSA from relevant components of key theoretical frameworks including Labor Process Theory, Critical Pedagogy, and Community-Based Participatory Research. These frameworks informed the design of activities such as critical reflection journals, media audits of health messaging on public and social platforms, and synchronous activities such as CIB-led campus tours for researchers. BSA “responses” informed the direction of subsequent sessions. The five BSAs were optional, and participants were compensated $15–$25 per activity. Feedback from research team debriefing sessions and CIB member evaluations guided BSA refinement and implementation. Results/Anticipated Results: CIB members completed nearly all BSAs and perceived their input as meaningfully shaping session and project goals. Evaluations and debriefing notes revealed that BSAs promoted continuity in participation, individual and group agency, and reflexivity among CIB members. Researchers noted improved depth, specificity, and sharing during CIB sessions and increased communication between CIB members and the research team regarding logistics, successes, and challenges related to BSA completion. Reported barriers to BSA implementation included competing demands (from family, work, and school) and inconsistent expectations for BSA completion. The research team addressed challenges by offering timeline flexibility, altering compensation structures and reflexively examining and discussing power disparities. Discussion/Significance of Impact: Translational research relies on responsive and sustainable community engagement. The BSA approach demonstrates how intentional, theory-informed activities can translate engagement principles into practice, maintaining meaningful and authentic collaboration beyond structured community meetings.

Objectives/Goals: The goal of this study was to determine if there are the peripheral immune system changes in persons undergoing treatment with lecanemab, and whether those changes are associated with the incidence of amyloid-related imaging abnormalities (ARIA), in order to identify strategies for improving the safety profile of anti-amyloid antibodies. Methods/Study Population: Three pairs of age, sex, APOE genotype, and infusion-matched individuals were selected from a study population at Norton Neuroscience Institute Memory Center (NNI-MC), based on the development of ARIA. Peripheral blood mononuclear cells isolated from these case–control subjects were collected and cryopreserved. Thawed cells were run through a deep sequencing pipeline, including single-cell RNAseq, CITEseq TCR clonality (V(D)Jseq), metabolomics, and lipidomics. Multi-omic data was integrated and analyzed using R (scRNAseq, CITEseq, and V(D)Jseq) and MetaboAnalyst (Metabolomics and Lipidomics). Deidentified clinical data was obtained to correlate to sequencing findings, including cognitive exam scores, MRI scans, and relevant lab values. Results/Anticipated Results: We noted an increase in lymphocytes in ARIA-positive subjects, with expansion in CD8+ T-cells – particularly CD45-RA expressing T-effector memory (TEMRA) subsets. These TEMRA cells expressed large clonal expansions in cells programmed for antigen response, cytotoxicity, and metabolic shifts. We found that in ARIA+ subjects, there was a pro-inflammatory glycolytic shift, accompanied by CD8-driven lipidomic changes. To determine potential effects of TEMRAs on the CNS, we mapped our data onto previously published ARIA+ patient brain transcriptomes (van Olst et al., 2025). TEMRA CD8s from our dataset showed increases in in silico communication with the vascular endothelium, indicating priming of these cells for cerebrovascular engagement. Discussion/Significance of Impact: Together, this first-of-its-kind data in human subjects receiving lecanemab infusions may point to peripheral immunity as a driving factor – and potential biomarker and therapeutic target for – ARIA pathogenesis, allowing for increased safety of these drugs in the highest risk AD populations.

Objectives/Goals: To benchmark the extent to which variability in automated WMH quantification influences stroke and dementia risk stratification by characterizing differences in volume estimates and spatial concordance across three segmentation tools (BIANCA, SAMSEG, and nnU-Net). Methods/Study Population: This retrospective, cross-sectional study will analyze de-identified clinical brain MRI scans from patients aged ≥50 years, including those with and without prior stroke, obtained from institutional imaging repositories. Eligible scans include co-registered T1-weighted and FLAIR sequences. Scans with poor image quality and scans from patients with multiple sclerosis, other demyelinating disorders, or brain tumors will be excluded. WMHs will be segmented using three tools: BIANCA (supervised k-NN classifier), SAMSEG (unsupervised Bayesian model), and nnU-Net (self-configuring deep learning). Agreement in WMH burden will be assessed with intraclass correlation coefficients (ICC[3,1]) and spatial concordance with Dice similarity coefficients (DSC). Bland-Altman analyses will evaluate bias. Results/Anticipated Results: We will analyze 600 brain MRI scans. All three tools are expected to detect similar WMH spatial distributions, though total WMH volume estimates may differ. nnU-Net may yield higher WMH burden estimates than BIANCA or SAMSEG. WMH burden will be neuroradiologist-adjudicated to establish the gold standard, against which concordance for each tool and inter-tool agreement will be assessed using ICC[3,1] and DSC. We anticipate moderate-to-high agreement, but tool-specific variability could influence reproducibility and interpretation of WMH burden for future WMH-related research studies. This study will quantify the extent of variability among tools in clinical brain MRI scans and evaluate whether these differences could meaningfully alter future stroke risk classification thresholds. Discussion/Significance of Impact: This study will benchmark automated segmentation tools for WMH-related research by standardizing WMH quantification to improve reproducibility and reliability for stroke and dementia risk stratification. Establishing tool-specific benchmarks may also guide integration of automated WMH measures into clinical decision-support systems.

Objectives/Goals: We aimed to explore informaticist experiences, lessons learned, and recommendations arising from their experiences during the Department of Veterans Affairs (VA) transition from its homegrown electronic health record (EHR) to a commercially developed system. Methods/Study Population: We conducted semi-structured Microsoft Teams interviews with 16 informaticists from five VA facilities and a regional office, representing roles that included nurse informaticists, program analysts, change management managers as well as chief roles in health informatics, medical informatics, nursing informatics, and quality, safety, and value. Interviews explored role transitions, staffing impacts, and lessons learned during the Oracle Cerner EHR implementation. Transcripts underwent matrix-based rapid qualitative analysis, with ongoing thematic analysis guided by Nicholson’s role transition framework. Results/Anticipated Results: Informaticists underwent major role transitions during EHR implementation. Pre-transition, their roles were largely technical, centering on updating and maintaining the EHR and providing as-needed user support. During the transition, informaticists became advocates, change leads, and trainers for frontline clinicians, performing relational and educational rather than technical work. Post-transition, roles shifted once again, encompassing frontline support, workflow education, and help ticket triage. With little external guidance, informaticists engaged in adaptive role innovation and identity redefinition throughout the transition, often experiencing role overload. Discussion/Significance of Impact: Findings highlight the need to better prepare and support workforce members whose evolving roles are central to others’ adaptation. Implementation planning must account for the education and training needs of these intermediary roles, not just their functional contributions.

Objectives/Goals: This initiative accelerates translational science by integrating infrastructure, engagement, and workforce development to overcome barriers and improve efficiency in building a new academic health center. The primary objective is to overcome translational roadblocks while fostering a culture of innovation and collaboration. Methods/Study Population: The Florida State University (FSU) Health Research Connections ticketing and navigation system serves as a centralized system for research support, data capture, and service coordination. Pilot projects include utilizing group model building, a systems science approach, to map barriers and facilitators in community clinics; and testing community-engaged, trustworthy recruitment strategies into FSU Health Research Link, a community facing research portal. The initiative engages academic researchers, community members, healthcare providers, and trainees across Florida, adapts existing UF/FSU hub resources, and develops new resources that capitalize on FSU’s strengths and needs. Evaluation applies the Translational Science Benefits Model to assess impact across domains. Results/Anticipated Results: Preliminary outcomes from CTSA-supported initiatives at FSU demonstrate measurable progress in breaking translational barriers and boosting efficiency. The HRC ticketing system has streamlined research support. Metrics to be presented include the number of individuals enrolled in the FSU Health Research Community Portal, use of HRC tools and services, engagement in training workshops, external funding and scholarly outputs, utilization of big data, and adoption of findings into clinical practice. Findings from community-engaged recruitment pilots and trust metrics will also be presented. Discussion/Significance of Impact: FSU Health offers a replicable model for new academic health centers, particularly relevant for newer CTSA hub partners. Embedding evaluation and engagement sets a new standard for community-partnered, efficient, and impactful translational science infrastructure and accelerates discovery into practice.

Objectives/Goals: The University of Illinois Chicago’s (UIC) Center for Clinical and Translational Science (CCTS) aimed to increase research dissemination findings to strengthen community relationships and opportunities for researchers to be in dialogue with communities. Methods/Study Population: From 2024 to present, the CCTS developed a collaboration with community and internal UIC partners connected to Auburn Gresham (AG), a predominantly African American community located in a far southside neighborhood of Chicago, to co-design a series of Community Forums. Community partners included UIC’s Neighborhood Center AG site, dedicated to supporting local stakeholders through strategic interventions, Office of Community Engagement and Neighborhood Health Partnerships (OCEAN-HP), fostering community partnerships through service, education, and research, and the Greater Auburn Gresham Development Corporation (GAGDC), a grassroots organization focused on community development in low-income communities. Results/Anticipated Results: Each community forum discussed research topics affecting the AG community by providing a safe space to foster bidirectional dialogue among community members, university researchers, and other field experts, while simultaneously bridging the gap between the community and research. Topics were identified through AG members’ community expertise and audience anecdotal/survey feedback. The forums were free and evolved to provide community resource packets and basic health screenings (e.g., blood pressure and glucose). This was an iterative engagement process (e.g., establishing relationships and trust, incorporating community feedback) to best mold the community forums to AG’s needs. The community audience and researchers/field experts have continued to express their interest in future community events. Discussion/Significance of Impact: The AG community forums have increased the interest in what research is and how to be involved among community members. Researchers/field experts have reiterated the importance of bidirectional dialogue with the community and the positive impact on research. These forums support research dissemination and community engagement.

Objectives/Goals: Previous studies of nanoparticles in a penetrating spinal cord injury demonstrated their ability to modulate immune responses to reduce secondary injury. This study investigated nanoparticles in contusion injury models, which is more common clinically, to quantify their efficacy as a function of injury severity. Methods/Study Population: Female C57BlJ/6 mice were anesthetized, and a T9/T10 laminectomy was performed to expose the spinal cord. A stereotactic setup with an Infinite Horizon impactor was used to position the spinal cord to receive a mild (30 kdyne), moderate (50 kdyne), or severe (70 kdyne) contusive injury. Within two hours of injury, poly(lactide-co-glycolide) nanoparticles, formed via a single emulsion with sonication protocol, or phosphate-buffered saline (PBS) vehicle were injected intravenously via tail vein injection. Nanoparticles (NPs) or PBS were administered daily for 7 days. Function was evaluated using the Basso Mouse Scale (BMS) test for hind limb function starting 3 days post-injury and repeated weekly thereafter, as well as a horizontal ladder beam test once they had recovered to at least occasional plantar stepping. Results/Anticipated Results: In the moderate injury group, NP-treated mice had significantly higher BMS scores starting 6 weeks post-injury (p=0.009), with the NP-treated mice performing better than control to the end of the study. In the mild injury group, NP-treated mice scored significantly better on BMS starting at 3 days post-injury (p=0.005). This difference persisted through week 4, and then the vehicle-treated mice maintained a slightly lower score through the end of the study. In the severe injury group, no significant difference on BMS scores was observed between groups and animals did not recover enough function to be evaluated via ladder beam. Scoring of the ladder beam test for moderate and mild injury studies is underway. Animals treated with NPs are anticipated to have a significantly higher number of successful steps compared to control. Discussion/Significance of Impact: Spinal cord injury consists of a primary injury and secondary injury caused by inflammation at the injury site. Nanoparticles present an opportunity to reduce secondary injury to improve functional outcomes of individuals with SCI. This study of NPs in a graded severity contusion model further illustrates the feasibility and utility of NPs in SCI.

Objectives/Goals: To showcase a survey tool designed to assess awareness, engagement, satisfaction, and unmet research needs across a six-institution CTSA. To demonstrate how findings can inform strategic planning, resource allocation, and continuous quality improvement (CQI). Methods/Study Population: The Institute for Translational Medicine (ITM) is a partnership of six Chicago-area institutions led by the University of Chicago and Rush University. Since 2023, the ITM has conducted an annual online survey assessing engagement, satisfaction, and unmet research needs related to ITM-sponsored activities. Administered via Qualtrics to faculty and staff over a four-week period, the 12-minute survey includes 1) questions to assess engagement and referral to ITM resources; 2) a Net Promoter Score (NPS) to measure satisfaction and likelihood of recommending the program, with scores above 0 interpreted as positive; and 3) a clinical and translational research needs inventory with a “top 3 pain points” ranking to identify unmet needs. Responses were analyzed by institution and translational research stage. Results/Anticipated Results: Response rates were 26% in 2023 and 20% in both 2024 and 2025. Familiarity with the ITM increased each year from 68% in 2023, to 73% in 2024, and 79% in 2025, though about one-fifth of respondents remain unfamiliar. Participation in at least one ITM-sponsored activity varied by respondent pool, from 59% in 2023 to 35% in 2024, and 38% in 2025. Referral rates to ITM-sponsored activities ranged from 11 to 45% depending on the activity across the three years. NPS scores were consistently higher among participants (2023: -20; 2024: -5; 2025: 0) versus non-participants (2023: -53; 2024: -49; 2025: -45). Across three years, respondents have indicated that “analyze data” is the number one greatest unmet research need, though needs and pain points vary by site over time. Discussion/Significance of Impact: NPS findings suggest that greater engagement with ITM resources is associated with higher satisfaction and likelihood of referral. Most participants report positive experiences, demonstrating program value. Challenges remain in reaching unfamiliar researchers, highlighting opportunities to expand awareness and address unmet research needs.

Objectives/Goals: Micro-credentials (MCs) are short programs to allow learners to gain knowledge and skills at their own pace. Since MCs do not have due dates, this study aims to understand the learners’ timing and duration to complete MCs through the Buffalo CTSI program in respect to semester-based academic calendar and other CTSI-based learning opportunities. Methods/Study Population: Buffalo CTSI offers four MCs in effective teaching, good clinical practice, responsible research, and scientific communication. The MCs were developed to enhance existing workforce development (WFD) series. Each series consists of 3-5 90-minute sessions offered annually during academic year. Learners can enroll in a corresponding skill-based MC at any point during or after completing the WFD series and have up to a year to complete the MC assignments. We identified 89 from 2019 to 2022 and 45 individuals who completed an MC through Buffalo CTSI between Spring 2024 -and Summer 2025 using data from the UB Office of micro-credentials. Data examined included time since completing the initial WFD series, number of days needed to complete an MC, number of revisions on assignments per awardee, and proportion of non-completers. Results/Anticipated Results: Overall, 85 learners completed an MC from 2019 to 2024 including 39 faculty/trainees, 26 students, and 20 staff. The median amount of time to complete an MC varied from 28 days (effective teaching) to 45 days for responsible research. The percent of learners needing to revise an MC assignment varied from 100% for effective teaching to 30% for scientific communication. The number of MC non-completers varied from 0% for effective teaching to 13% for responsible research. Out of 45 learners who completed MCs from 2024 to 2025, 28 completed MCs synchronously and 17 completed MCs using the new asynchronous format. Discussion/Significance of Impact: MC flexibility in timing and feedback allows learners to complete MCs throughout the year even if they participated in the WFD series asynchronously. Constructive MC feedback can assist learners in revising their work to reach proficiency. Adapting the schedule for completion helps learners obtain desired skills that fits individual timelines.

Objectives/Goals: Mainstream interest in psilocybin (“shrooms”) has grown over the past several years as two US states have “legalized” psilocybin and clinical trials are underway for use in multiple mental health conditions. We evaluated self-reported use of psilocybin among community members in North Florida and trends in use from 2012 to the present. Methods/Study Population: Established in 2011 as part of the UF Clinical and Translational Science Institute, UF HealthStreet Community Health Workers enroll community members from North Florida through outreach, conduct Health Needs Assessments (HNAs) to guide referrals to social and medical services and opportunities to participate in research. This model provides community health concerns and behaviors, which can be compared across time to direct resources based on importance to the community. Topics covered in the HNA include demographics, trust in research and researchers, food insecurity, access to medical care, health conditions, and use of various substances, from legal drugs like alcohol or opioid pain medication to illicit substances such as cocaine, heroin, or psilocybin. Results/Anticipated Results: We evaluated self-reported use of psilocybin among HealthStreet members, beginning in 2012 when psilocybin use was first assessed until October 2025. Among 12,870 participants, 1,194 (9.3%) reported using psilocybin in the past 12 months. Males were more likely to endorse psilocybin use than females (57.3% vs 42.7%), and Whites were overwhelmingly more likely to report psilocybin use (76.1%) than Blacks (12.1%) or People of Other Race (11.8%). Prevalence of psilocybin use was roughly equal to self-reported rates of use of speed or amphetamines (9.2%), but slightly higher than rates of use of ADHD medications (8.5%). Most notably, when viewed over time, self-reported psilocybin use tripled, from 6% in 2012–2013 to 18% in 2024–2025. Discussion/Significance of Impact: These community data from North Florida confirm a rapidly growing use of psilocybin across the United States. Risk factors for psilocybin use such depression, anxiety, and other drug use will be evaluated. Knowledge of current trends is essential to tailor public health messages and inform both providers and policymakers.

Objectives/Goals: CYP2D6 is a highly polymorphic gene with significant pharmacogenomic and public health implications. Understanding the population distribution of CYP2D6 variants offers opportunities to enhance awareness of its pharmacological relevance, particularly across different ancestral groups. Methods/Study Population: Using the National Institute of Health All of Us Research Program Data Browser, we identified CYP2D6 alleles with a frequency ≥0.10 in the overall cohort or within ancestral subgroups and classified in ClinVar as having either “drug response” or ”undefined” significance. We assessed alleles with frequency differences ≥0.10 between ancestral subgroups and the overall population to highlight subgroup-specific pharmacogenomic relevance. CYP2D6alleles with ClinVar “undefined” significance classifications were cross-referenced with expert pharmacological resources (e.g., PharmVar) to identify candidates for reclassification as ”drug response” significance variants. Results/Anticipated Results: Among the 89CYP2D6 alleles with ClinVar “drug response” significance classification, 7 of 11 (64%) were more frequent (≥ 0.10) in the East Asian ancestry subgroup compared to the overall population. Among 6,262 CYP2D6 alleles with “undefined” significance classification, 60 showed elevated frequencies (≥ 0.10) in at least one subgroup: Middle Eastern (31/60; 52%), African (22/60; 37%), East Asian (21/60; 35%), and South Asian (4/60; 7%) ancestries. Of these 60 alleles, 16 (27%) map to 41–76, star allele haplotypes that contain at least one sub-allele with “definitive” allele evidence level in PharmVar. Discussion/Significance of Impact: Our findings highlight the differential distribution and potential pharmacological relevance of CYP2D6 alleles across ancestral subgroups. These insights support potential reclassification of certain ”undefined” alleles and highlight the importance of inclusive pharmacogenomic research.

Objectives/Goals: This study aimed to explore the feasibility of implementing sustainable depression screening services within an appointment-based pharmacy. Methods/Study Population: A qualitative observational study was conducted at a rural, appointment-based community pharmacy. Following staff consent, unstructured interviews and workflow observations were performed over a 1-week period to characterize existing processes and identify potential integration points for screening. Field notes from observations and interviews were supplemented with an anonymous Qualtrics survey assessing perceived barriers and facilitators to depression screening implementation. Results/Anticipated Results: The pharmacy filled approximately 200 prescriptions daily and employed one pharmacist and two to four bilingual technicians serving a predominantly Hispanic population. Both the pharmacist and staff were supportive of implementing depression screening and viewed it as compatible with their MedSync workflow. Reported barriers included the lack of a private counseling area and concerns regarding patient comfort discussing mental health due to stigma. Discussion/Significance of Impact: Appointment-based pharmacies are well suited to integrate depression screening into existing workflows. Future implementation efforts should address structural and stigma-related barriers to optimize feasibility and sustainability.