The conservation of plant genetic resources for food and agriculture (PGRFA) is essential for global food and nutrition security, climate resilience and sustainable agriculture. This study presents a monitoring and evaluation (M&E) framework applied across 20 countries in the Global South to assess the state of ex situ PGRFA conservation. Using mixed methods, we collected quantitative and qualitative data on genebank performance, validated operational status, and identified specific needs of genebanks. Our findings demonstrate the practical use of this M&E framework for genebanks to assess efficiency and effectiveness in genebank management, while also guiding targeted capacity development and context-specific interventions to address persistent challenges. We recommend adopting the approaches described in this study, including genebank reviews and the review checklist, to support more systematic and evidence-based assessment of genebanks. Aligning national efforts with global conservation objectives will help generate actionable recommendations to strengthen genebanks and ensure the long-term conservation and availability of PGRFA.

The global ex situ conservation system reflects the collective efforts of individual genebanks, each influenced by its unique history, opportunities for acquisition and user demands. As a result, acquisition activities in most genebanks have been opportunistic rather than strategic to support representation of national crop diversity or driven by clearly identified conservation priorities.

The objective of this study was to characterize the composition of national collections in 20 countries within the global ex situ conservation system. It assessed the representation of crop genetic diversity that originated nationally within the global system and evaluated the composition balance, as it relates to duplication and gaps in conservation. Across these 20 countries, numerous genebanks are conserving large number of accessions for only a few genera, while a much smaller number of genebanks are conserving fewer than 10 accessions from a broader range of genera. The findings indicate that ex situ conservation, both nationally and globally, has significant potential duplications and gaps in the genetic resource coverage.

Individual institutes in the global system must carefully reconsider the composition of their collections, especially given the significant costs and limited financial and human resources available for long-term ex situ conservation. As national genebanks and the global system become increasingly important resources for the future, it will be essential for genebanks to ensure that the composition of their collections meets future demands.

This study examined data from 20 national genebanks in Latin America and the Caribbean, Africa, the Near East and Asia to identify similarities and differences in genebank operations and processes, funding and facilities, as well as opportunities to strengthen their contributions to the global system of crop conservation and use. Data on genebank performance metrics were collected and used to assess compliance with FAO Genebank Standards. This enabled the analysis of trends in ex situ conservation of major food crops, locally important crops and crop wild relatives across national genebanks and the identification of shared challenges and opportunities to improve genebank operations and address funding gaps. All genebanks in the study failed to meet quality management standards and performance goals for the effective management of crop collections. The most pressing challenge for all national genebanks was the management and safety duplication of highly diverse collections in both seed and field genebanks, often with limited information available to guide best conservation practices. A further critical constraint was the fluctuating and often insufficient funding to support the wide range of tasks needed to secure and use this valuable national crop germplasm.

Genebanks worldwide play a crucial role in the long-term conservation and accessibility of wide diversity of plant genetic resources (PGR). These resources are essential for addressing current and future challenges related to climate change and biodiversity loss, thereby contributing to agricultural production and sustainability. Enhancing the efficiency of genebanks requires implementing a quality management system that ensures data transparency, as well as the documentation and standardization of processes. This study proposes a set of 10 mandatory and 38 optional genebank metrics specifically designed for collections of orthodox seeds. These metrics define a set of basic parameters that are easy to calculate and collectively serve as a valuable tool for improving genebank management, enhancing performance, fostering transparency and promoting collaborations among institutions. Fourteen institutions engaged in the long-term conservation of PGR were consulted in the development of the proposed metrics. This work lays the foundation for the establishment of a widely accepted set of genebank metrics within the global genebank community. Further research and iterative development are necessary to formulate comprehensive metrics applicable to other ex situ conservation methods (such as cryopreservation, in vitro and field conservation) as well as in situ conservation strategies (including genetic reserves and on-farm conservation).

The Genebank Standards for Plant Genetic Resources recommend that genebanks periodically monitor the change in viability of their stored germplasm to ensure timely regeneration. The Australian Grains Genebank (AGG) has been recording germination data on orthodox seeds from tropical grain crops, stored under long-term storage (−20°C), for 40 years. Real-time viability data, collated from germination tests carried out on seedlots which had been in storage for a minimum of 20 years, from six agriculturally important grain crops (Sorghum bicolor, Phaseolus vulgaris, Glycine max, Vigna radiata, Cajanus cajan and Vigna angularis) was analysed by probit analysis. For each species independently, a common loss in viability was observed for all seedlots showing a consistent decline in viability during storage; with observed longevity estimates (σ; time for viability to fall by 1 NED/probit) of 17.4, 30.7, 33.2 49.6, 48.8, 63.5 and years for Vigna angularis, Cajanus cajan, Phaseolus vulgaris, Glycine max, Sorghum bicolor, and Vigna radiata, respectively. Common values of σ were subsequently used to determine species-specific viability monitoring intervals, based on the results of the last germination test. Dynamic monitoring intervals are a cost-efficient strategy that will avoid the over-use of seed through too frequent viability monitoring whilst still ensuring the timely regeneration of material. With funding shortfalls often reported as the main contributing factor to regeneration and viability testing backlogs, the ability for genebanks to maximise cost-efficiencies, where possible, is paramount to secure the genetic integrity of stored germplasm; particularly as collections continue to grow.

Plant viruses cause considerable damage in agriculture through high losses in yield or productivity, and quality of crops. Unfortunately, there are few direct control mechanisms in place for viruses and very few protocols for eliminating them due to the wide diversity of viruses. Some viruses of clonal crops are easily removed through meristem culture, but many others are more intricately associated with the meristematic cells or localized in the plant genome where they pose significant risks to plant health and productivity, thus require eradication. Viruses such as Yam Mosaic Virus (YMV) and Yam Mild Mosaic Virus (YMMV) damage the economic and food security benefits provided by the tropical clonal crops. This report presents an overview of the viral challenges of tropical clonal crops. It also provides information on the state of current knowledge of various sanitation options available against viruses affecting the clonal crops. This report provides a reference to genebanks for early accurate detection and elimination of viruses among in vitro-grown tropical plants which, is a major step towards the improvement of the quality of clonal germplasm stored or distributed by the concerned genebanks. The information brought to light in this report could lead to technical refinement of the existing procedures or creation of new protocols for virus-infected germplasm.

Since collecting and conservation efforts for plant genetic resources began in earnest a century ago, genebanks and stored accessions have proliferated, irrationally so. Many smaller national genebanks are falling considerably short of their mandates to conserve diversity and promote its use. Combined and functioning as intended, the International Treaty, the Crop Trust and the Svalbard Global Seed Vault create the context in which these genebanks could safely and positively transition to focus on facilitating experimentation and use by their farmers. There is precedent for a creative approach in the massive distribution of seeds to farmers in the U.S. in the 1800s that enabled the introduction, spread and adaptation of many crops. Progress will require resolution of the Treaty’s ‘access and benefit-sharing’ impasse, which could be advanced by an embrace of the Treaty’s provisions, more collaboration for conservation, and a facilitated shift in focus to the distribution and use of genetic resources, especially by smaller genebanks and for ‘opportunity crops’ of primary interest to developing countries.

National genebanks offer diverse collections of locally adapted crops which can support farmers’ climate resilience, nutritional security and economic innovations, yet are often overlooked in climate adaptation strategies. Across much of the world, national genebanks are unknown to farmers, or poorly connected for varietal exchange. This paper examines the impacts of establishing ‘Germplasm User Groups’ (GUGs) across five African countries to connect farmers with genebanks as rapid responders to local agricultural challenges. GUGs conducted farmer participatory research to evaluate genebank materials and establish pathways for the exchange of knowledge and crop diversity in farming communities. Drawing on surveys and interviews from over 1,600 smallholders, we found GUGs increase farmer understanding of genebanks, improve access to crop diversity and increase farmer exchanges with national genebanks. As well as material exchange, smallholders welcome the learning opportunities from GUGs to address local farming challenges. On average, GUG members share genebank seed with four other farmers, demonstrating the potential spillover effects of this model for sharing crop diversity. We close with recommendations to improve the working of GUGs and offer guidance for other countries looking to adopt the system as a rapid approach strategy to build local resilience in the face of climate change.

Crop and varietal diversification are essential for African smallholder farmers to adapt to the complex and unprecedented challenges posed by climate change. Although African genebanks maintain seed collections of numerous crops, with thousands of varieties collected from their countries’ farmers, the direct use of these collections by farmers is very limited. Five African national genebanks therefore explored ways to strengthen farmers’ access to and use of these collections through a longer-term collaborative process. The genebanks and their partners engaged with ‘Germplasm User Groups’ as a basis for facilitating sustained joint learning with farmers for use of conserved germplasm. The structure of these groups and the methods they used for identifying and testing germplasm accessions, although differing by country context, all enabled a diversity of farmers to learn about a wide range of germplasm under relevant field conditions. The large number of accessions that farmers selected, their diverse advantages and the requests by numerous farmer groups to continue exploring additional crops and varieties indicated the usefulness of these approaches. These experiences revealed the feasibility and unique roles and opportunities for national genebanks to facilitate farmers’ direct use of the diversity conserved in their crop collections. National genebanks thus have unique responsibilities for adapting their operating procedures and partnering with research and development practitioners to facilitate farmers’ discovery and use of their conserved crop diversity.

National genebanks hold vast crop diversity that could support more resilient agri-food systems, yet their collections remain underutilized due to weak linkages with users. To address this, Kenya’s national genebank piloted the Germplasm User Group (GUG) model to facilitate structured engagement with farmers, researchers and other stakeholders. The objective of this study is to gain understanding on the feasibility and utility of this approach in supporting farmers in accessing and uncovering new and promising crop diversity. In this activity, 5 participatory variety selection (PVS) field days were held where farmers evaluated 2,041 sorghum accessions, out of which 393 accessions were selected as having farmer-preferred traits. Results demonstrate that farmer selection was non-random, as it resulted in accessions with significantly different trait profiles. Useful traits such as tolerance to striga and early maturity were identified. Seeds of 51 accessions were shared with 514 households belonging to 26 GUGs for on-farm evaluation. Out of these, farmers selected 46 accessions for seed saving and showed significantly greater preference for genebank accessions compared to the check variety. Partnerships were developed with sorghum breeding and research teams, farmer groups, extension service and marketers. This study has highlighted the value of agro-morphological characterization and passport data in assembling germplasm subsets with useful traits. The study has further shown that PVS and farmer-managed evaluation provide a powerful pathway through which farmers can discover and access the otherwise ‘hidden’ diversity conserved in genebank seed collections.

There are increasing calls for greater availability of plant genetic resources (PGR) for use in plant breeding to help counter the adverse impacts of a changing climate and threats from pests and diseases, particularly in a context of reduced agricultural inputs, and the needs of the increasing human population. Managing and promoting ex situ and in situ conservation of PGR requires an effective data and informatics foundation. However, in situ data management is particularly undeveloped, especially when compared to ex situ documentation. The work presented here is a consolidated descriptor list to support in situ conservation of PGR. The consolidated PGR descriptor list is based on numerous partial lists collated from historic PGR activities, biodiversity conservation and protected area networking activities. New descriptors for in situ conservation activities were developed where gaps were identified. The draft consolidated PGR descriptor list was reviewed and revised through a process of consultation with experts in PGR documentation and in situ conservation. In total, 171 descriptors were identified, of which seven are defined as mandatory, 47 are defined as core descriptors and 29 are newly developed descriptors for in situ conservation of PGR. The descriptors cover all aspects of in situ conservation, from gathering passport data (for which there are already well-established descriptors), to monitoring and managing crop wild relative and landrace populations. Recommendations are made for the most effective use and future development of descriptors and how they could be used to further develop and support in situ PGR conservation implementation.